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The map label for this gene is wecB [C]

Identifier: 170754425

GI number: 170754425

Start: 3345598

End: 3346656

Strand: Reverse

Name: wecB [C]

Synonym: CLD_1440

Alternate gene names: 170754425

Gene position: 3346656-3345598 (Counterclockwise)

Preceding gene: 170757348

Following gene: 170755005

Centisome position: 84.55

GC content: 28.9

Gene sequence:

>1059_bases
ATGAAGATATTAACAGTAGTTGGTGCACGTCCACAATTTATAAAAGCAGCAGCAGTTTCAAATATAATAAGAAAAGAGCA
TGAAGAAATATTAGTTCATACTGGTCAACATTATGATGAAAATATGTCTAAAATATTTTTTGAAGAGTTAAGAATACCTA
AGCCAGATTATAATTTAGAAATAGGTTCAGGTAATCATGGTGAACAGACAGGTAAAATGCTTATAGAATTAGAAAAAATA
TATTTAAAAGAAAAGCCAGATTTAGTATTAGTCTATGGGGATACAAATTCTACCTTAGCAGGAGCTTTGTGTGCAAGTAA
ACTGTTAATACCTGTAGCTCATGTAGAAGCTGGTCTTAGAAGCTTTAATATGAATATGCCAGAAGAACAAAATAGAATTC
TTACAGATCATATATCAAAGTTATTATTTGTACCTACAGTTACAGCAGAAAAAAATCTTCATACTGAGGGAGTTAATAAC
GGTGTTCATAATGTAGGAGATGTAATGTTTGATGCTGTATTACATTTCAAGAAATTAGCAGAAGAGAAGGAAAGTATACT
AGATAAAATCTCTGTAAAATCTGGAGAATATATTTTAACAACAATACATAGAGCAGAAAACACTAATGATATAAATAGAT
TGAAAAATATAATAGAGGCATTAAATGAAAGTGGAAAGTCAATAGTATTACCACTTCATCCAAGAACTAAAAAATATATG
GAAGATTATAATTTACAATTTAATGATAATATAAAATTAATAGAGCCAGTAGGATATCTTGATATGATTACTTTAGAAAT
GAATTCACAAAAGATAGTTACTGATAGTGGTGGAGTTCAAAAAGAAGCTTTCTTTATGGAAAAACCTTGTGTTACTATGA
GAGATGAAACAGAGTGGGTAGAGACCGTAGAAAATGGATGGAATGTAATAGTAGGTACAAATAAAGAAAAAATATTAAAT
GCTATAATAAATTTTAAGCCTGAAAAAGAACAGCAAGAAATATTCGGAAATGGTCATGCAGCGGAAAAAATATTAGATAT
AATAAACGAAATGAAGTAA

Upstream 100 bases:

>100_bases
AGACAGGAAGAAAGTTTGTGGAAGATAATTTTAATATAGAAGATAATTTTAATAATGTAGATATAATATACAAATCTATA
ATAGATGGAGGAGATAATTA

Downstream 100 bases:

>100_bases
TTATTTTGAGGTGATAATATGAAAATCCTATTTTTGACTCAATACTGTCCACCTGAAGTTGGAGCACCTCAAAACAGAAT
TTTTGAGTTTGCAAAGCAAC

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHEEILVHTGQHYDENMSKIFFEELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNN
GVHNVGDVMFDAVLHFKKLAEEKESILDKISVKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYM
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNVIVGTNKEKILN
AIINFKPEKEQQEIFGNGHAAEKILDIINEMK

Sequences:

>Translated_352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHEEILVHTGQHYDENMSKIFFEELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNN
GVHNVGDVMFDAVLHFKKLAEEKESILDKISVKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYM
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNVIVGTNKEKILN
AIINFKPEKEQQEIFGNGHAAEKILDIINEMK
>Mature_352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHEEILVHTGQHYDENMSKIFFEELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNN
GVHNVGDVMFDAVLHFKKLAEEKESILDKISVKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYM
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNVIVGTNKEKILN
AIINFKPEKEQQEIFGNGHAAEKILDIINEMK

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=377, Percent_Identity=33.9522546419098, Blast_Score=147, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 39865; Mature: 39865

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILTVVGARPQFIKAAAVSNIIRKEHEEILVHTGQHYDENMSKIFFEELRIPKPDYNLE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCCEEE
IGSGNHGEQTGKMLIELEKIYLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLR
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNNGVHNVGDVMFDAVLHFKKLA
HCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EEKESILDKISVKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYM
HHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWV
HHCCCEECCCEEEECCCCCEEEEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCHHHH
ETVENGWNVIVGTNKEKILNAIINFKPEKEQQEIFGNGHAAEKILDIINEMK
HHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILTVVGARPQFIKAAAVSNIIRKEHEEILVHTGQHYDENMSKIFFEELRIPKPDYNLE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCCEEE
IGSGNHGEQTGKMLIELEKIYLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLR
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVNNGVHNVGDVMFDAVLHFKKLA
HCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EEKESILDKISVKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYM
HHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
EDYNLQFNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWV
HHCCCEECCCEEEECCCCCEEEEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCHHHH
ETVENGWNVIVGTNKEKILNAIINFKPEKEQQEIFGNGHAAEKILDIINEMK
HHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA