Click here to switch to the map view.

The map label for this gene is purC

Identifier: 170756658

GI number: 170756658

Start: 3115635

End: 3116339

Strand: Reverse

Name: purC

Synonym: CLD_1663

Alternate gene names: 170756658

Gene position: 3116339-3115635 (Counterclockwise)

Preceding gene: 170757832

Following gene: 170755843

Centisome position: 78.73

GC content: 29.22

Gene sequence:

>705_bases
ATGGAAAAGAAGGATATGTTATATGAAGGAAAGGCTAAAAAAATATTTGGAACGGATGATAAGGATACAGTTGTTGTGTA
TTATAAAGATGATGCCACAGCTTTTAATGGGGAGAAGAAAGGAACTATTGAAGATAAGGGAGTTATGAATAACTCTATAA
CAGCAATGTTATTTGAACTTTTAGAAAAAAAAGGTGTAAAAACACATTTCATAGAAAAGATAAATGAAAGAGAACAGCTT
TGCAAAAAAGTAGAAATAGTTCCACTAGAAGTTATAGTTAGAAATATAGCAGCAGGAAGTATGGCAAAAAGATTAGGACT
TTCAGAAGGAAGAAAATTAGATACTACTGTATTTGAAATAAGTTATAAAAATGATGATTTAAATGACCCTCTTATAAATG
ACTATCATGCAGTGGCTATAGGACTTACAACCTTTGCAGAACTAAAAGAAATGTATTCTATAGCAGAAAAAGTAAATAAT
ACATTAAAAGAATTCTTTGATGAGCAAGGAATAAATTTAGTTGATTTTAAAATAGAAATAGGAAGATTTAATGGTGAGCT
CCTTTTAGCAGATGAAATATCCCCAGATACTTGTAGATTATGGGATAAAAGCACAGGAGAAAAGTTAGATAAAGATAGAT
TTAGAAGAGACATGGGAAATGTAAAAGAAGCTTATATGGAAATATTGAAAAGAGTCAATAAATAA

Upstream 100 bases:

>100_bases
TATCTTTAAAGTATGATGAATTAGAAGAAAAACTTATAGAATATAGAAAAGATATGAAAGAAAAATTTATAAATGATAAC
AAAGAGGGAGTGGAGTTATA

Downstream 100 bases:

>100_bases
ATTGTTATAGTTTAAAATTATTTTTGCAGCAGCTTGGGATAATCTAAAAGAAGATTAAACTTTAAAAATAGAAAATAAGT
GGGGAGTAAAAATGAGTATG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MEKKDMLYEGKAKKIFGTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFELLEKKGVKTHFIEKINEREQL
CKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEISYKNDDLNDPLINDYHAVAIGLTTFAELKEMYSIAEKVNN
TLKEFFDEQGINLVDFKIEIGRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK

Sequences:

>Translated_234_residues
MEKKDMLYEGKAKKIFGTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFELLEKKGVKTHFIEKINEREQL
CKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEISYKNDDLNDPLINDYHAVAIGLTTFAELKEMYSIAEKVNN
TLKEFFDEQGINLVDFKIEIGRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK
>Mature_234_residues
MEKKDMLYEGKAKKIFGTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFELLEKKGVKTHFIEKINEREQL
CKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEISYKNDDLNDPLINDYHAVAIGLTTFAELKEMYSIAEKVNN
TLKEFFDEQGINLVDFKIEIGRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=242, Percent_Identity=33.4710743801653, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI5453539, Length=242, Percent_Identity=33.4710743801653, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI119220559, Length=249, Percent_Identity=32.1285140562249, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1788820, Length=232, Percent_Identity=40.0862068965517, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17531275, Length=232, Percent_Identity=34.4827586206897, Blast_Score=109, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24583917, Length=247, Percent_Identity=27.5303643724696, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI18860083, Length=235, Percent_Identity=25.1063829787234, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 26811; Mature: 26811

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01057 SAICAR_SYNTHETASE_1 ; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKDMLYEGKAKKIFGTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFEL
CCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHH
LEKKGVKTHFIEKINEREQLCKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEI
HHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
SYKNDDLNDPLINDYHAVAIGLTTFAELKEMYSIAEKVNNTLKEFFDEQGINLVDFKIEI
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
GRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK
EECCCEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKKDMLYEGKAKKIFGTDDKDTVVVYYKDDATAFNGEKKGTIEDKGVMNNSITAMLFEL
CCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHH
LEKKGVKTHFIEKINEREQLCKKVEIVPLEVIVRNIAAGSMAKRLGLSEGRKLDTTVFEI
HHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
SYKNDDLNDPLINDYHAVAIGLTTFAELKEMYSIAEKVNNTLKEFFDEQGINLVDFKIEI
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
GRFNGELLLADEISPDTCRLWDKSTGEKLDKDRFRRDMGNVKEAYMEILKRVNK
EECCCEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA