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The map label for this gene is purF

Identifier: 170755843

GI number: 170755843

Start: 3114095

End: 3115543

Strand: Reverse

Name: purF

Synonym: CLD_1664

Alternate gene names: 170755843

Gene position: 3115543-3114095 (Counterclockwise)

Preceding gene: 170756658

Following gene: 170756492

Centisome position: 78.71

GC content: 32.37

Gene sequence:

>1449_bases
ATGAGTATGTGCTATATGTTAGATGATTTAAATGAAAATATGCCCTTTGATTTAGAAGGAGATAAGTTTAAAGAAGAATG
TGGGGTATTTGGAGTATTTTCTAAAGATAATGAATCAAAGTCAGCAGAAATAACTTATTACGGATTATATGCTCTTCAAC
ATAGGGGACAAGAAAGTGCAGGAATAGTGGTATCTGATGGAGAAAAGTTTAAATATCATAAGGGGATGGGTCTTGTATCA
GATGTTTTTAGTAAGGAAACCATAGAAGGATTAAGAGGAAATTCTGCTATAGGGCATGTTAGATATTCCACTACAGGAGC
TAGTAAATCAGATAATGCACAACCTATAGTAGGTACTTATAAATTAGGCTCTATTGCCATAGCCCATAATGGTAATTTAG
TTAATGCAGCAGTTATAAGAGAACTTTTAGAAGATGGTGGATGTATTTTTCAAACTTCTATAGATACAGAGGTATTATTA
AATTTAATAGCAAGAAGCGCTAAAAAAGGTATAGATAAAGCAGTAGTAGATGCAATACAAGCAATTAAAGGTTCCTATGC
TATTGTTATACTTACAGAAGATAAGTTAATAGGAGCACGTGATCCTCATGGTATAAGACCTATATGCCTGGGGAAAATTG
GGGATGATTATTTACTAAGCTCAGAAAGTTGTGCTTTTGATTGTGTAGGTGGAGAGTTTATAAGAGATATAGAACCTGGA
GAAATAGTTATTATAGATGAAAGTGGAATAAATTCAATTAAGTTTGCAGAAAAAACAAAATGTCATACCTGTGCTTTTGA
ATATATATATTTTGCAAGACCAGATAGCGCTATGGATGGTATAAATGTTTATGAATCAAGGGTTAGAGCGGGAAGAAAAC
TCTATGAAGAATATCCAGTAGAGGCAGATATAGTTATAGGAGTTCCAGATTCAGGTATACCAGCTGCAGTAGGATATGCT
GAAGCTTCAGGAATACCTTATGGTATAGGATTTATAAAAAATAAATATGTAGGAAGAACTTTTATAGCACCTTCTCAAGA
ATTAAGAGAAAAAGCTGTATCTGTAAAACTAAATCCACTTAAAATAAATGTAGAAGGAAAAAGAGTAGTAATAATAGATG
ATTCCATAGTAAGAGGAACTACTAGTAAAAGATTAGTACAAATATTAAGAAAAGCTGGAGCTAAGGAAGTTCATTTTAGA
GTATCATCTCCAGTGGTTAAATATCCTTGTTATTTTGGAATAGATACTCCTTATAGAAAAGATTTAATAGGTGCTCACTC
AGAAGTAGAAGAAATAAGAGAAAAAATAGGAGCAGATAGTCTAGCTTATATAAGTATGGAAGGCTTAGTAGAAACACTTA
ATAAAGATAAAGGCTTTTGCTTGGGATGTTTTAATGGTATATATCCTATATCTGCACCTATAGAAATGCCTAAAGATAGT
TTGGAATAG

Upstream 100 bases:

>100_bases
AATAAATAAATTGTTATAGTTTAAAATTATTTTTGCAGCAGCTTGGGATAATCTAAAAGAAGATTAAACTTTAAAAATAG
AAAATAAGTGGGGAGTAAAA

Downstream 100 bases:

>100_bases
AGGATTTAAGTTGTTCTAGCTTTAGGTAGAAAAAATTTATTTCTTTGTTTGACACTAAATATACGATTCATATTTAATCG
ATTATTTTTTCATGATCCTA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSMCYMLDDLNENMPFDLEGDKFKEECGVFGVFSKDNESKSAEITYYGLYALQHRGQESAGIVVSDGEKFKYHKGMGLVS
DVFSKETIEGLRGNSAIGHVRYSTTGASKSDNAQPIVGTYKLGSIAIAHNGNLVNAAVIRELLEDGGCIFQTSIDTEVLL
NLIARSAKKGIDKAVVDAIQAIKGSYAIVILTEDKLIGARDPHGIRPICLGKIGDDYLLSSESCAFDCVGGEFIRDIEPG
EIVIIDESGINSIKFAEKTKCHTCAFEYIYFARPDSAMDGINVYESRVRAGRKLYEEYPVEADIVIGVPDSGIPAAVGYA
EASGIPYGIGFIKNKYVGRTFIAPSQELREKAVSVKLNPLKINVEGKRVVIIDDSIVRGTTSKRLVQILRKAGAKEVHFR
VSSPVVKYPCYFGIDTPYRKDLIGAHSEVEEIREKIGADSLAYISMEGLVETLNKDKGFCLGCFNGIYPISAPIEMPKDS
LE

Sequences:

>Translated_482_residues
MSMCYMLDDLNENMPFDLEGDKFKEECGVFGVFSKDNESKSAEITYYGLYALQHRGQESAGIVVSDGEKFKYHKGMGLVS
DVFSKETIEGLRGNSAIGHVRYSTTGASKSDNAQPIVGTYKLGSIAIAHNGNLVNAAVIRELLEDGGCIFQTSIDTEVLL
NLIARSAKKGIDKAVVDAIQAIKGSYAIVILTEDKLIGARDPHGIRPICLGKIGDDYLLSSESCAFDCVGGEFIRDIEPG
EIVIIDESGINSIKFAEKTKCHTCAFEYIYFARPDSAMDGINVYESRVRAGRKLYEEYPVEADIVIGVPDSGIPAAVGYA
EASGIPYGIGFIKNKYVGRTFIAPSQELREKAVSVKLNPLKINVEGKRVVIIDDSIVRGTTSKRLVQILRKAGAKEVHFR
VSSPVVKYPCYFGIDTPYRKDLIGAHSEVEEIREKIGADSLAYISMEGLVETLNKDKGFCLGCFNGIYPISAPIEMPKDS
LE
>Mature_481_residues
SMCYMLDDLNENMPFDLEGDKFKEECGVFGVFSKDNESKSAEITYYGLYALQHRGQESAGIVVSDGEKFKYHKGMGLVSD
VFSKETIEGLRGNSAIGHVRYSTTGASKSDNAQPIVGTYKLGSIAIAHNGNLVNAAVIRELLEDGGCIFQTSIDTEVLLN
LIARSAKKGIDKAVVDAIQAIKGSYAIVILTEDKLIGARDPHGIRPICLGKIGDDYLLSSESCAFDCVGGEFIRDIEPGE
IVIIDESGINSIKFAEKTKCHTCAFEYIYFARPDSAMDGINVYESRVRAGRKLYEEYPVEADIVIGVPDSGIPAAVGYAE
ASGIPYGIGFIKNKYVGRTFIAPSQELREKAVSVKLNPLKINVEGKRVVIIDDSIVRGTTSKRLVQILRKAGAKEVHFRV
SSPVVKYPCYFGIDTPYRKDLIGAHSEVEEIREKIGADSLAYISMEGLVETLNKDKGFCLGCFNGIYPISAPIEMPKDSL
E

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=514, Percent_Identity=38.9105058365759, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=40.5117270788913, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1790167, Length=231, Percent_Identity=30.7359307359307, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17554892, Length=477, Percent_Identity=40.6708595387841, Blast_Score=343, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6323958, Length=470, Percent_Identity=38.936170212766, Blast_Score=292, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24659598, Length=489, Percent_Identity=41.3087934560327, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI28573187, Length=510, Percent_Identity=40.7843137254902, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24659604, Length=464, Percent_Identity=42.2413793103448, Blast_Score=376, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52648; Mature: 52517

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMCYMLDDLNENMPFDLEGDKFKEECGVFGVFSKDNESKSAEITYYGLYALQHRGQESA
CCEEEEECCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCEEEEEEEEEEEHHCCCCCC
GIVVSDGEKFKYHKGMGLVSDVFSKETIEGLRGNSAIGHVRYSTTGASKSDNAQPIVGTY
CEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEE
KLGSIAIAHNGNLVNAAVIRELLEDGGCIFQTSIDTEVLLNLIARSAKKGIDKAVVDAIQ
EECEEEEEECCCEEHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHH
AIKGSYAIVILTEDKLIGARDPHGIRPICLGKIGDDYLLSSESCAFDCVGGEFIRDIEPG
HHCCCEEEEEEECCCEECCCCCCCCCCEEEECCCCCCEECCCCCEEEECCCHHHHCCCCC
EIVIIDESGINSIKFAEKTKCHTCAFEYIYFARPDSAMDGINVYESRVRAGRKLYEEYPV
EEEEEECCCCCCEEECCCCCHHEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
EADIVIGVPDSGIPAAVGYAEASGIPYGIGFIKNKYVGRTFIAPSQELREKAVSVKLNPL
CEEEEEECCCCCCCCEECCHHCCCCCEEHHHHHCCCCCEEECCCCHHHHHHHHEEEEEEE
KINVEGKRVVIIDDSIVRGTTSKRLVQILRKAGAKEVHFRVSSPVVKYPCYFGIDTPYRK
EEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCEEECCCCCHHH
DLIGAHSEVEEIREKIGADSLAYISMEGLVETLNKDKGFCLGCFNGIYPISAPIEMPKDS
HHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
LE
CC
>Mature Secondary Structure 
SMCYMLDDLNENMPFDLEGDKFKEECGVFGVFSKDNESKSAEITYYGLYALQHRGQESA
CEEEEECCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCEEEEEEEEEEEHHCCCCCC
GIVVSDGEKFKYHKGMGLVSDVFSKETIEGLRGNSAIGHVRYSTTGASKSDNAQPIVGTY
CEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEE
KLGSIAIAHNGNLVNAAVIRELLEDGGCIFQTSIDTEVLLNLIARSAKKGIDKAVVDAIQ
EECEEEEEECCCEEHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCHHHHHHHHHH
AIKGSYAIVILTEDKLIGARDPHGIRPICLGKIGDDYLLSSESCAFDCVGGEFIRDIEPG
HHCCCEEEEEEECCCEECCCCCCCCCCEEEECCCCCCEECCCCCEEEECCCHHHHCCCCC
EIVIIDESGINSIKFAEKTKCHTCAFEYIYFARPDSAMDGINVYESRVRAGRKLYEEYPV
EEEEEECCCCCCEEECCCCCHHEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
EADIVIGVPDSGIPAAVGYAEASGIPYGIGFIKNKYVGRTFIAPSQELREKAVSVKLNPL
CEEEEEECCCCCCCCEECCHHCCCCCEEHHHHHCCCCCEEECCCCHHHHHHHHEEEEEEE
KINVEGKRVVIIDDSIVRGTTSKRLVQILRKAGAKEVHFRVSSPVVKYPCYFGIDTPYRK
EEEECCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCEEECCCCCHHH
DLIGAHSEVEEIREKIGADSLAYISMEGLVETLNKDKGFCLGCFNGIYPISAPIEMPKDS
HHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
LE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]