| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is amiC [H]
Identifier: 170756098
GI number: 170756098
Start: 3358275
End: 3361169
Strand: Reverse
Name: amiC [H]
Synonym: CLD_1430
Alternate gene names: 170756098
Gene position: 3361169-3358275 (Counterclockwise)
Preceding gene: 170755605
Following gene: 170757747
Centisome position: 84.92
GC content: 29.46
Gene sequence:
>2895_bases ATGAATTCTGGGAAAATGTTTAGATTTAGAGAAAGGAGGGGAAATAAGAAAATTATGAAGATAAAAAAGATATTTATAAA CTTTGTGGTAATAATTTTTATTAGTACCATTATAAACGGTGTATATTCATCTAAAGTTGATGCTAGTAGTACTACCACTA TAATAGGACAAGCTGAATTAACTCAAGAACAAGCATTAAATTATTTTAGATCTAGAAATAGTGAAAAGAGTGACCAAGAC ATAAAAGATTTTATTTCTATAGTTTGGCAGGAAGCTAATTTAGAAGGAATAAGAGCTGATGTAGTTTTTATGCAAATAAT GAAAGAAACAAATTTTTTGAGATTTACAGGAGATGTAAAAGAATGTCAAAATAATTTTGCAGGTATTGGTGCTACAGGTG ATGGTGTAGCAGGTGCTTATTTTAAAGATACAAGAACAGGAGTAAGAGCCGTTGTTCAACATCTAAAAGCTTATTGTAGT ACTGAGGGATTAAAAAATCCTTGTGTAGATCCACGATTTACATATGTTCAAAGAGGAATAAGTCCCTATGTTGAGTGGCT AGGTATAGGGGAAAATCCTAATTATCCAGATAAAGGATGGGCTGCAGATAATAATTATGGTAAGAGTATAGTTGAAATGA TGAACAGTGCAAAGTATCTGGCAAATGAAGGTGATTCACAAGGAAATATAACTACTTCAAAGGCTACTATTAATAATTTA GAAGTAAGCTTAGATGGAAATAAGGTGACAACCAATGAATTAGAACCAGGAAAATCATATAACATAAAAGCTTATGGAAA TAGTTCTAATGGAGTATTATATGAATATTGGATAAAAGACTTATCAATTAATTCATGGATAAAGCTAAGAGATTATAGTA CAACCAATGAAGTTAAATGGACACCAAATAAATCAGGAAAATATCTAATAGGGGTACACGTTAAAGATAGATATAGTAAA GAAAGATTAGATAATTTTAAGTATGTAGAATATAATGTAGCATCCCTTAAAAAAGCTACTATTAGCAGCTTAGAAGTAAG CTTAGATGGAAATAATGTAGTGAATAATGAATTACAACTAGGTAAGACATATAATATAAAAGCTTATGGCAGTAGCTCTA ATGGCGTATTGTATGAATATTGGATAAAAGACTTATCAAAGAATTCATGGATGAAGTTAAGAGATTATAGTACAAGTACT CAAGTCACATGGACATCAAATAAATCAGGAAAGTATCTATTAGGAGTACATGTTAAAGATAGGTATAGTGAAGAAAGGTT AGATAACTTTAAGTATGTAGAATACAACGTAGCATCACCTAAAAAGGCTACTGTTAATAGCTTAGAAGTAATCTTAGATG GAAATAAGGTAGTAACTAATGAATTAGATCCAGGAAAATCATATAACATAAAAGCTTATGGAAATAGTTCTAATGGAGTA TTATATGAATATTGGATAAAAGACTTATCAATTAATTCATGGATAAAGTTAAAAGATTATAGTACAAGTACTCAAGTTAC ATGGACACCAAATAAGCCAGGGAAATATTTAATAGGGGTACACGTAAAGGATAAATATAGTAAAGAAAAATTAGATAATT TTAAATATGTAGATTATAATGTAACATCACCTAAAAAGGCTAATATCAATAGTTTAGAAGTAAGTCTAGATGGAGCTAAA GTAGTAAATAATGAACTACAAGCAGGTGAAATATATAATATAAAAGCTTATGGTAGTAGTTCTAATGGAGTATTGTATGA ATACTGGATAAAAGACTTATCAATTAATTCATGGATAAAGTTAAAAGATTATAGTACAAGTACTCAAGTTACATGGACAC CAAATAAATCAGGAAAATATTTAATAGGGGTACACATAAAAGATAAATATAGTACAGAAAAATTAGATAATTACAAATAT GTAGAATATAATGTGAAACTATCTAAAAAAGCAGTTATTAGTAACTTGGAAGTAAGCCTAAATGGAAAGATAATAACAAA CAATCAATTAGATGCAGGAAAAACATATAGTATAAAAGCTTGCGGGGAAAGTCTTAATGGAGTATTGTATGAATACTGGA TAAAAGATTTATCAATTAATTCATGGATAAAGTTAAAAGATTATAGTACAAGTACTCAAGTTACATGGACACCAAATAAA GCAGGAAAATATCTAATAGGAGTACATGCAAAGGATAAATATAGTAATGAAAGATTAGATAACTATAAATATGTAGAGTA TAGCGTTAAAGGTGGTTTAATAAAAACTATAGTTTTAGATGCAGGCCATGGAGGAAGAGACTCAGGTGCAGTGTCTTCTG CAGCAACTAGAAATATACACGAAGCAGATATAGTTCAGAAAATTACCATAAAGTTAGGAAACCTTCTTAAATCCAAAGGA TATAATGTTATCTATACTAGAGATAAGATAGATCTTTATAATTATCCTTCAATAACACAAAATTTAGAAGATAGAATAAA TGTAGCTAATAGTATAAAAGCTGATTTATTTATGAGCATACATGCTGACTCTGCTGATAGTTCATCAGCTAATGGATATG GTGCGCACTATAGCACATATAGACCTAATTTAGATAATTCAGGAGTATATAAAGAGGGTGATGTGTGGTATGATAGAACT CCTTGTGATGCAGCATTAAAAAGCAAAGTGTTATCACAACTTATAGTAAATGAGATGTCTTCTTTAGGAGGATCAAATAG GGGTATAGAGGATCACAATTTATATGTTACAAGAAATGCCTTAATGCCTTCTGTTCTTGTAGAAACTGGATTTGTTTCTA ATGATACAGAAGTTAGGAAGTTAAATAGTGACTCTTATCAAAATCAAATAGCTCAAAAACTTTACAATGCAGTAACAAAA TTATTTAGCATGTAG
Upstream 100 bases:
>100_bases CAATTAATTCCATGATTGGTGATTTTTTTTATTTTGTGTAATATATAAATAAAAATTTAAATTAACATATGATATAATAA TTTACAAAGGAGGTGAATTT
Downstream 100 bases:
>100_bases TTAAAAAACGTTGGCATGTCCTTTGCCAACGTTTTTTTGTTTTACTCATAAGCTTTAAATGATATTTTCTATTTATTGTA TATATGATAAAATAAAGATA
Product: putative N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 964; Mature: 964
Protein sequence:
>964_residues MNSGKMFRFRERRGNKKIMKIKKIFINFVVIIFISTIINGVYSSKVDASSTTTIIGQAELTQEQALNYFRSRNSEKSDQD IKDFISIVWQEANLEGIRADVVFMQIMKETNFLRFTGDVKECQNNFAGIGATGDGVAGAYFKDTRTGVRAVVQHLKAYCS TEGLKNPCVDPRFTYVQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMNSAKYLANEGDSQGNITTSKATINNL EVSLDGNKVTTNELEPGKSYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSK ERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLGKTYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTST QVTWTSNKSGKYLLGVHVKDRYSEERLDNFKYVEYNVASPKKATVNSLEVILDGNKVVTNELDPGKSYNIKAYGNSSNGV LYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKPGKYLIGVHVKDKYSKEKLDNFKYVDYNVTSPKKANINSLEVSLDGAK VVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHIKDKYSTEKLDNYKY VEYNVKLSKKAVISNLEVSLNGKIITNNQLDAGKTYSIKACGESLNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNK AGKYLIGVHAKDKYSNERLDNYKYVEYSVKGGLIKTIVLDAGHGGRDSGAVSSAATRNIHEADIVQKITIKLGNLLKSKG YNVIYTRDKIDLYNYPSITQNLEDRINVANSIKADLFMSIHADSADSSSANGYGAHYSTYRPNLDNSGVYKEGDVWYDRT PCDAALKSKVLSQLIVNEMSSLGGSNRGIEDHNLYVTRNALMPSVLVETGFVSNDTEVRKLNSDSYQNQIAQKLYNAVTK LFSM
Sequences:
>Translated_964_residues MNSGKMFRFRERRGNKKIMKIKKIFINFVVIIFISTIINGVYSSKVDASSTTTIIGQAELTQEQALNYFRSRNSEKSDQD IKDFISIVWQEANLEGIRADVVFMQIMKETNFLRFTGDVKECQNNFAGIGATGDGVAGAYFKDTRTGVRAVVQHLKAYCS TEGLKNPCVDPRFTYVQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMNSAKYLANEGDSQGNITTSKATINNL EVSLDGNKVTTNELEPGKSYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSK ERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLGKTYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTST QVTWTSNKSGKYLLGVHVKDRYSEERLDNFKYVEYNVASPKKATVNSLEVILDGNKVVTNELDPGKSYNIKAYGNSSNGV LYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKPGKYLIGVHVKDKYSKEKLDNFKYVDYNVTSPKKANINSLEVSLDGAK VVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHIKDKYSTEKLDNYKY VEYNVKLSKKAVISNLEVSLNGKIITNNQLDAGKTYSIKACGESLNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNK AGKYLIGVHAKDKYSNERLDNYKYVEYSVKGGLIKTIVLDAGHGGRDSGAVSSAATRNIHEADIVQKITIKLGNLLKSKG YNVIYTRDKIDLYNYPSITQNLEDRINVANSIKADLFMSIHADSADSSSANGYGAHYSTYRPNLDNSGVYKEGDVWYDRT PCDAALKSKVLSQLIVNEMSSLGGSNRGIEDHNLYVTRNALMPSVLVETGFVSNDTEVRKLNSDSYQNQIAQKLYNAVTK LFSM >Mature_964_residues MNSGKMFRFRERRGNKKIMKIKKIFINFVVIIFISTIINGVYSSKVDASSTTTIIGQAELTQEQALNYFRSRNSEKSDQD IKDFISIVWQEANLEGIRADVVFMQIMKETNFLRFTGDVKECQNNFAGIGATGDGVAGAYFKDTRTGVRAVVQHLKAYCS TEGLKNPCVDPRFTYVQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMNSAKYLANEGDSQGNITTSKATINNL EVSLDGNKVTTNELEPGKSYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSK ERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLGKTYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTST QVTWTSNKSGKYLLGVHVKDRYSEERLDNFKYVEYNVASPKKATVNSLEVILDGNKVVTNELDPGKSYNIKAYGNSSNGV LYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKPGKYLIGVHVKDKYSKEKLDNFKYVDYNVTSPKKANINSLEVSLDGAK VVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHIKDKYSTEKLDNYKY VEYNVKLSKKAVISNLEVSLNGKIITNNQLDAGKTYSIKACGESLNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNK AGKYLIGVHAKDKYSNERLDNYKYVEYSVKGGLIKTIVLDAGHGGRDSGAVSSAATRNIHEADIVQKITIKLGNLLKSKG YNVIYTRDKIDLYNYPSITQNLEDRINVANSIKADLFMSIHADSADSSSANGYGAHYSTYRPNLDNSGVYKEGDVWYDRT PCDAALKSKVLSQLIVNEMSSLGGSNRGIEDHNLYVTRNALMPSVLVETGFVSNDTEVRKLNSDSYQNQIAQKLYNAVTK LFSM
Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI87082163, Length=229, Percent_Identity=31.0043668122271, Blast_Score=93, Evalue=7e-20, Organism=Escherichia coli, GI1788776, Length=238, Percent_Identity=28.1512605042017, Blast_Score=77, Evalue=6e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR021731 - InterPro: IPR002508 [H]
Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 108688; Mature: 108688
Theoretical pI: Translated: 9.43; Mature: 9.43
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNSGKMFRFRERRGNKKIMKIKKIFINFVVIIFISTIINGVYSSKVDASSTTTIIGQAEL CCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHH TQEQALNYFRSRNSEKSDQDIKDFISIVWQEANLEGIRADVVFMQIMKETNFLRFTGDVK HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCHH ECQNNFAGIGATGDGVAGAYFKDTRTGVRAVVQHLKAYCSTEGLKNPCVDPRFTYVQRGI HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC SPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMNSAKYLANEGDSQGNITTSKATINNL CHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECEEEEEEE EVSLDGNKVTTNELEPGKSYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKW EEEECCCEEECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEE TPNKSGKYLIGVHVKDRYSKERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQL CCCCCCCEEEEEEECCHHCHHHHCCCEEEEEEHHHHHHHHHEEEEEEECCCCEECCCEEC GKTYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQVTWTSNKSGKYLLGVHVKD CCEEEEEEECCCCCCEEEEEEHHHCCCCCCEEEEECCCCEEEEEECCCCCCEEEEEEEHH RYSEERLDNFKYVEYNVASPKKATVNSLEVILDGNKVVTNELDPGKSYNIKAYGNSSNGV CCCHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEECCCCCCE LYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKPGKYLIGVHVKDKYSKEKLDNFKYVDYN EEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCEEEEEEECCCCCHHHHCCEEEEEEE VTSPKKANINSLEVSLDGAKVVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIK CCCCCCCCCCEEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEE LKDYSTSTQVTWTPNKSGKYLIGVHIKDKYSTEKLDNYKYVEYNVKLSKKAVISNLEVSL EEECCCCEEEEECCCCCCCEEEEEEEECCCCCHHCCCEEEEEEEEEECHHHHHEEEEEEE NGKIITNNQLDAGKTYSIKACGESLNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNK CCEEEECCCCCCCCEEEEEECCCCCCCCEEEEHHHHCCCCCEEEEEECCCCCEEEECCCC AGKYLIGVHAKDKYSNERLDNYKYVEYSVKGGLIKTIVLDAGHGGRDSGAVSSAATRNIH CCCEEEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCHHHHCCCH EADIVQKITIKLGNLLKSKGYNVIYTRDKIDLYNYPSITQNLEDRINVANSIKADLFMSI HHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCHHHCHHHHHHHHHCCCEEEEEEE HADSADSSSANGYGAHYSTYRPNLDNSGVYKEGDVWYDRTPCDAALKSKVLSQLIVNEMS ECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHHH SLGGSNRGIEDHNLYVTRNALMPSVLVETGFVSNDTEVRKLNSDSYQNQIAQKLYNAVTK HCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHH LFSM HHCC >Mature Secondary Structure MNSGKMFRFRERRGNKKIMKIKKIFINFVVIIFISTIINGVYSSKVDASSTTTIIGQAEL CCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHH TQEQALNYFRSRNSEKSDQDIKDFISIVWQEANLEGIRADVVFMQIMKETNFLRFTGDVK HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCHH ECQNNFAGIGATGDGVAGAYFKDTRTGVRAVVQHLKAYCSTEGLKNPCVDPRFTYVQRGI HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC SPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMNSAKYLANEGDSQGNITTSKATINNL CHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECEEEEEEE EVSLDGNKVTTNELEPGKSYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKW EEEECCCEEECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEE TPNKSGKYLIGVHVKDRYSKERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQL CCCCCCCEEEEEEECCHHCHHHHCCCEEEEEEHHHHHHHHHEEEEEEECCCCEECCCEEC GKTYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQVTWTSNKSGKYLLGVHVKD CCEEEEEEECCCCCCEEEEEEHHHCCCCCCEEEEECCCCEEEEEECCCCCCEEEEEEEHH RYSEERLDNFKYVEYNVASPKKATVNSLEVILDGNKVVTNELDPGKSYNIKAYGNSSNGV CCCHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEECCCCCCE LYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKPGKYLIGVHVKDKYSKEKLDNFKYVDYN EEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCEEEEEEECCCCCHHHHCCEEEEEEE VTSPKKANINSLEVSLDGAKVVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIK CCCCCCCCCCEEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEE LKDYSTSTQVTWTPNKSGKYLIGVHIKDKYSTEKLDNYKYVEYNVKLSKKAVISNLEVSL EEECCCCEEEEECCCCCCCEEEEEEEECCCCCHHCCCEEEEEEEEEECHHHHHEEEEEEE NGKIITNNQLDAGKTYSIKACGESLNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNK CCEEEECCCCCCCCEEEEEECCCCCCCCEEEEHHHHCCCCCEEEEEECCCCCEEEECCCC AGKYLIGVHAKDKYSNERLDNYKYVEYSVKGGLIKTIVLDAGHGGRDSGAVSSAATRNIH CCCEEEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCHHHHCCCH EADIVQKITIKLGNLLKSKGYNVIYTRDKIDLYNYPSITQNLEDRINVANSIKADLFMSI HHHHHHHHHHHHHHHHHHCCCEEEEEECCEEECCCCCHHHCHHHHHHHHHCCCEEEEEEE HADSADSSSANGYGAHYSTYRPNLDNSGVYKEGDVWYDRTPCDAALKSKVLSQLIVNEMS ECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHHHHHHH SLGGSNRGIEDHNLYVTRNALMPSVLVETGFVSNDTEVRKLNSDSYQNQIAQKLYNAVTK HCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHH LFSM HHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10710307 [H]