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The map label for this gene is epsC [H]

Identifier: 170757747

GI number: 170757747

Start: 3356232

End: 3358097

Strand: Reverse

Name: epsC [H]

Synonym: CLD_1431

Alternate gene names: 170757747

Gene position: 3358097-3356232 (Counterclockwise)

Preceding gene: 170756098

Following gene: 170754485

Centisome position: 84.84

GC content: 26.96

Gene sequence:

>1866_bases
ATGAGAAAAGATAGGGAATTGATTCTTTATGATATATTATTAATAATTTTATCATTATATTTAGCAATATTATTAAGATT
CGATTTTGGTATGGCTTCTATGGCTTCGGAATTTAAAAAATATATGATATTTTTTAAGTTATCAGTAATTCCAGTTATAA
TAATAATCTTATTTTTTAATAAAATATTTAATTTATATAGTAGTATATGGAAATATGCTTCAGTAGAAGAACTTATGTCT
ATAATTTATTCAGTTTCTATATCTAATATTATATTCATAATTTATAGTTATTTTATTAATTATAAAATTCTAGAAAGTAA
TTATTATAGATTCCCATTTACTGTACATATAATATTTTGGATTTTATCCATAGTATCTCTAGGAGGTACAAGATTTATAT
ATAGAGTTATAGAAGATAAGGAATGTAATAAGAACAAATGTAACTGTGATTCTAAAGAAAAGAAGTTACTCATAATAGGG
GCAGGAGATGCAGCAGCCCTTATTATAAAAGAAATAAAAAGAAATAGGGAATTAAATTATAGTATAATAGGTCTTATAGA
TGATGACATAGAGAAGTTAGGAAAGAGAATAAATGGTATACCTGTTTTAGGCGGAAGAGAAAGTATTATAAAGATATCTA
AAAATAAAAAAATAGATGAAATAATATTAGCTATACCTTCAGCATCATCTTCAACAAAAAGTGAAATAGTATCTATATGT
AAAGAAACTAGTTGTAAATTAAAAACTTTACCAAGCATGGATAAGGTAATACAGGGTAAATTTAATATGAGTAAGTTAAG
AGATGTAAGTATTGAAGATTTGCTTGGGAGAGAGGAAGTTAAGCTAGATGATAGTGGAATAAATAAATATATAAAGGATA
AAGTAGTTTTAGTTACTGGTGGAGGGGGATCTATTGGGTCAGAACTTTGCAGACAAATAGTTAAATTTTCACCTAAGAAA
CTTTTAATATTAGATATATATGAAAACAATGCCTATGATGTTCAAATGGAATTAAATTATAAATATCCAGAGCTAGATAA
AGCTGTTATAATAGCTTCCATAAGGGATGAAAAGAGATTAAAAGATATATTTTCATCATATAAGCCAGATGTAGTTTTTC
ATGCAGCAGCACATAAACATGTTCCACTAATGGAAGAGAATCCAGCAGAATCTATTAAAAATAATGTAATAGGAACCTAT
AATCTAATAAAATGTGCCCACGAATTTGGAACTGAGCGTTTTGTACAAATAAGTACAGACAAAGCTGTTAATCCAACTAA
TATAATGGGAGCTACAAAAAGATTTTGTGAAATAATGATTCAAGCTTTTGATAAAATAAGTAAGACTGAGTTTGTAGCAG
TTAGATTTGGGAATGTTTTAGGAAGTAATGGATCTGTTATTCCATTATTTAAAAAACAAATAGCTCATGGAGGTCCTGTT
ACTGTAACCCATCCAGAAATAAATAGATATTTTATGACCATACCAGAAGCAGCTCAATTAGTTATACAAGCAGGAGCTAT
GGCAAAAGGTGGAGAAATATTTGTTTTGGATATGGGAAGGCCAGTTAAAATTGTAGATTTAGCTAAAGACTTAATAACCT
TATCTGGTTATAAGCCAGGGGAGGATATTAAAATTGAGTATACAGGATTAAGACCTGGAGAAAAATTATATGAAGAATTA
TTGATGGATGAAATAGCTTTAACATCAACAGGACATAATAAAATATTTGTAGAAAAACCTATGGAAAATAATATAGACTT
TGTAGAAAAATCCATAGAAGAATTTAAGAAAGTTGTTTATAATGATAAAGAAGAAATTTTTGCATTAATACAAAAGAAAG
TTCCAACATATATAAGAAAAAAATAA

Upstream 100 bases:

>100_bases
GTATATATGATAAAATAAAGATATTGCAAAAAATTGTTGAATAATTTTATCAATGATGTAAAATAAATAAGTAGAGTTTA
GAGTTTAAGGAAGTGATTAT

Downstream 100 bases:

>100_bases
TGCTTAATAAATAATAGATAAGCTTTATGTACAAAATTTAAATATAAAATAATAGTAGCTATAAAATAGTGACAAAAAAT
CAAAAATATATAAATTAAGC

Product: exopolysaccharide biosynthesis protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNRELNYSIIGLIDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSASSSTKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINKYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRLKDIFSSYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGTERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQIAHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGRPVKIVDLAKDLITLSGYKPGEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVYNDKEEIFALIQKKVPTYIRKK

Sequences:

>Translated_621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNRELNYSIIGLIDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSASSSTKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINKYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRLKDIFSSYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGTERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQIAHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGRPVKIVDLAKDLITLSGYKPGEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVYNDKEEIFALIQKKVPTYIRKK
>Mature_621_residues
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFNKIFNLYSSIWKYASVEELMS
IIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFWILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIG
AGDAAALIIKEIKRNRELNYSIIGLIDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSASSSTKSEIVSIC
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINKYIKDKVVLVTGGGGSIGSELCRQIVKFSPKK
LLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRLKDIFSSYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTY
NLIKCAHEFGTERFVQISTDKAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQIAHGGPV
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGRPVKIVDLAKDLITLSGYKPGEDIKIEYTGLRPGEKLYEEL
LMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVYNDKEEIFALIQKKVPTYIRKK

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=265, Percent_Identity=29.0566037735849, Blast_Score=85, Evalue=3e-16,
Organism=Escherichia coli, GI48994969, Length=140, Percent_Identity=32.1428571428571, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788353, Length=140, Percent_Identity=32.1428571428571, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 70547; Mature: 70547

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIFNLYSSIWKYASVEELMSIIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFW
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEECCHHHHHHHH
ILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIGAGDAAALIIKEIKRNRELNY
HHHHHHCCCHHEEHEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCE
SIIGLIDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSASSSTKSEIVSIC
EEEEEECHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHH
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINKYIKDKVVLVTG
HHCCCCEECCCCHHHHHCCCCCHHHHCCCCHHHHCCCCCEEECHHHHHHHHCCCEEEEEC
GGGSIGSELCRQIVKFSPKKLLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRL
CCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCHHHH
KDIFSSYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTYNLIKCAHEFGTERFVQISTD
HHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECC
KAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQIAHGGPV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCEEHHHHHHHCCCCCE
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGRPVKIVDLAKDLITLSGYKPG
EEECCCCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCC
EDIKIEYTGLRPGEKLYEELLMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVY
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
NDKEEIFALIQKKVPTYIRKK
CCHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MRKDRELILYDILLIILSLYLAILLRFDFGMASMASEFKKYMIFFKLSVIPVIIIILFFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIFNLYSSIWKYASVEELMSIIYSVSISNIIFIIYSYFINYKILESNYYRFPFTVHIIFW
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCEECCHHHHHHHH
ILSIVSLGGTRFIYRVIEDKECNKNKCNCDSKEKKLLIIGAGDAAALIIKEIKRNRELNY
HHHHHHCCCHHEEHEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCE
SIIGLIDDDIEKLGKRINGIPVLGGRESIIKISKNKKIDEIILAIPSASSSTKSEIVSIC
EEEEEECHHHHHHHHHHCCCEEECCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHH
KETSCKLKTLPSMDKVIQGKFNMSKLRDVSIEDLLGREEVKLDDSGINKYIKDKVVLVTG
HHCCCCEECCCCHHHHHCCCCCHHHHCCCCHHHHCCCCCEEECHHHHHHHHCCCEEEEEC
GGGSIGSELCRQIVKFSPKKLLILDIYENNAYDVQMELNYKYPELDKAVIIASIRDEKRL
CCCCHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCEEEEEECCCHHHH
KDIFSSYKPDVVFHAAAHKHVPLMEENPAESIKNNVIGTYNLIKCAHEFGTERFVQISTD
HHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECC
KAVNPTNIMGATKRFCEIMIQAFDKISKTEFVAVRFGNVLGSNGSVIPLFKKQIAHGGPV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCEEHHHHHHHCCCCCE
TVTHPEINRYFMTIPEAAQLVIQAGAMAKGGEIFVLDMGRPVKIVDLAKDLITLSGYKPG
EEECCCCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCC
EDIKIEYTGLRPGEKLYEELLMDEIALTSTGHNKIFVEKPMENNIDFVEKSIEEFKKVVY
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
NDKEEIFALIQKKVPTYIRKK
CCHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]