| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is amiA [C]
Identifier: 170755605
GI number: 170755605
Start: 3361349
End: 3364252
Strand: Reverse
Name: amiA [C]
Synonym: CLD_1429
Alternate gene names: 170755605
Gene position: 3364252-3361349 (Counterclockwise)
Preceding gene: 170755028
Following gene: 170756098
Centisome position: 84.99
GC content: 28.13
Gene sequence:
>2904_bases ATGAAGGATAAAAAATATTATTTAGTACCTAAGATTATTTCAATATTTTTTATGTTATTTTTATTTTCTATAAAAGCACA AGCTGTATCGAATATAAGTATTAATTTAAAAACACCAACAGATCAAAACATAATAAATAGTAGTGAGTTAAATATTGATG GAGAAATAAAATCCATTTTAAAGTTAAAATCAGTATATTTATATATAGATAATGAAAATATAGGACAAGCTAATTTAGAT GAGTTAGAATTTAAAGAAAATACGTATAAAGCTAGATTAAAATATACAAAAAATATTTCTTCTTTAAAAAATGGAATACA TAATTTAAGAATTTTGGCTATAGATGAACAGAATAACAAAGAAGAAAAAGAAATTAGTATAAATATAAAAAGAGATCAAG AAAAATCAGAAACTAATAAAAATACTATACAGAATAATTTTGTAGATACTACTGTAAAGATGGGGAGTATAGAAAAATCA TTAACCACTGTATCGAATGAAGCTAAACTAGAAAAAGTAGAAGTTTCATACAATGGTAATGTGGTAACTAATGGAGAAAT GGGAGTTGGAAAAAGCTACGTTATAAAGGGATATGGAAATTCTGCAAATGGAGTATTGTATCAATTTTGGGTAAAGGATT TATCTACAAACAGTTGGACAATGATAAGGGATTATGGGGAAACAAATAGTTTTAATTACACACCAGCAAAGGCTGGAAAG TATCTGATAGGAATACATGTAAAAGATAAGTATAGTAAAGAAAATTTAGATGATTTTATATATGAAAATTATACAGTAAC TATAAGTAAAGCTAAACTAGAAAAAGTAGAAGTTTCATACAATGGTAATGTGGTAACTAATGGAGAAATGGGAGTTGGAA AAAGCTACCTTATAAAGGGATATGGAAATTCTGAAAATGGAGTACTGTATCAATTTTGGGTAAAGGATCTATCTACAAAC AGTTGGACAATGATAAGAGATTATGGAGAAACAAATAGTTTTAATTACACACCAACAAAGGCTGGAAAGTATCTTATAGG AATACATGTAAAAGATAAGTATAGTAAAGAAAATTTAGATGATTTTATATATGAGAATTATACAGTAACTGTAAGTAAAG CTAAATTAGAAAAAGTGGAAGTTTCATACGATGGTAATGTAATAACTAATGGAGAAATAGGAGTTGGAAAAAGCTACGTT ATAAAAGGATATGGAAATTCTGAAAATGGAGTACTGTATCAATTTTGGGTAAAAGATCTATCCACAAATAGTTGGACAAT GATAAGAGATTATGGAGAAACAAATAGTTTTAATTACACACCAACAAAGGCTGGAAAGTATCTTATAGGAATACATGTAA AAGATAAGTATAGTAAAGAAAATTTAGATGATTTTATATATGAAAATTATGATGTATCTATAAGCAAAGCTAAACTAGAA AAAGTAGAAGTTTCATACAATGGTAGCGTGATAACTAATGGAGAAATAGGAGTTGGAAAAAACTACGTTATAAAAGGATA TGGAAATTCTGCAAATGGAGCATTGTATCAATTTTGGGTAAAGGATTTATCTACAAACAGTTGGACAATGATAAGAGATT ATGGAGAAACAAATAGTTTTAATTATACACCAGCAAAGGATGGAAAGTATTTAATAGGAATACATGTAAAAGATAAGTAT AGTAAAGAGAATTTAGATGATTTTATATATGAGAATTATACAGTAACTATAAGCAAAGCTAAATTAGAAAAAGTAGAGGT TTCATATAACGGTAATGTGGTAACCAATGGAGAAATAGGAGTTGGAAAAAGCTACGTTATAAAAGGATATGGAAATTCTG CAAATGGAGTATTGTATCAATTTTGGGTAAAGGATCTATCTACAAACAGTTGGACAATGATAAAAGATTATGGAGAAGAG AATAGTATAAATTGGAAACCTACTGTTGGTGGAAAATATTTAATAGGGATACATGTAAAGGATAAATATAGTATCGATAC ATTAGATGATTTTATATATGAGAATTATACAATATTGAGTGATAATGCAAGGCTAGAAAAGCTAGAAGTTAAATTAGATG GAAACTTAATAAGTAATAATTTAAATGTAGGAAAGACATATACAATAGAAGGAAGTGCTACATCTAAAAATGGAGTGTTA TATCAATTTTGGGTAAAAGACTTGTCTATAAATAGCTGGGCAATGTTAAGGGATTATGGAGAATCAAATAATATAACATA TACACCAACAAAGAGTGGGCAATATTTAATAGGGGTACATGTAAAAGATAAGAATAGCAAGGAAAGTTTAGATGATTTTA AATACAATCAATATAGCATTATAGGTTATAATAAAACAGTAATTGTTGATGCAGGACATGGAGGATATGATAGTGGCGCT GTAGGACCAACCGGTATTAAAGAAAAGGATGTCACTTTAAAAGTAGCCTTAAAGTTAGGAAAAATTTTAGAAAATAGTGG GGTAAATGTTATTTATACTAGAACTAGTGATAATGTATCTTGGCCTTCAAATGAATCACAAGATTTAGCAGCTAGAGTGG CCATTGCAAATAACAATAATACAGATTTATATGTAAGTATACATGCTAATAGTTTTAATGGATCAGCGAATGGAACAGAA ACATATTACTATGATGGGAGTGCAAAAGGAAAAGAAACAGCAGAAAAAGTTCAAAAGGAACTTGTAAATTCTATTGGCTT ATATGATAGAGGCGTTAAAACAGCAGGATATTATGTTTTAAAAAATACTATAAGCCCATCTATATTGGTTGAATTAGGAT TCATAGATAATAGAAATGAAGAAATTCTTTTAAATTCAGATTGGTTCCAACAAAAGTGCGCAGAGGCTATTGCTAAAGGA ATATTAGGGACTAGCTTTATGTAA
Upstream 100 bases:
>100_bases AAATAATAAACATTATTATAATTTTATTTCAAGTATAGAATTGTTTATAGGGATCACGATAAGTAAATCTATAAAAATAA TTATTGAAAGGACTGAGGAG
Downstream 100 bases:
>100_bases ATTAATAAAGAAAAGGATAATTAATTATAAAAATTTTATTAACTTCTAGTTAAAGTGTTTTTAAAGTATAAAAAATCATC AATTAATTCCATGATTGGTG
Product: putative N-acetylmuramoyl-L-alanine amidase
Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]
Alternate protein names: NA
Number of amino acids: Translated: 967; Mature: 967
Protein sequence:
>967_residues MKDKKYYLVPKIISIFFMLFLFSIKAQAVSNISINLKTPTDQNIINSSELNIDGEIKSILKLKSVYLYIDNENIGQANLD ELEFKENTYKARLKYTKNISSLKNGIHNLRILAIDEQNNKEEKEISINIKRDQEKSETNKNTIQNNFVDTTVKMGSIEKS LTTVSNEAKLEKVEVSYNGNVVTNGEMGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGK YLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEMGVGKSYLIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGSVITNGEIGVGKNYVIKGYGNSANGALYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKDGKYLIGIHVKDKY SKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEIGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEE NSINWKPTVGGKYLIGIHVKDKYSIDTLDDFIYENYTILSDNARLEKLEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILGTSFM
Sequences:
>Translated_967_residues MKDKKYYLVPKIISIFFMLFLFSIKAQAVSNISINLKTPTDQNIINSSELNIDGEIKSILKLKSVYLYIDNENIGQANLD ELEFKENTYKARLKYTKNISSLKNGIHNLRILAIDEQNNKEEKEISINIKRDQEKSETNKNTIQNNFVDTTVKMGSIEKS LTTVSNEAKLEKVEVSYNGNVVTNGEMGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGK YLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEMGVGKSYLIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGSVITNGEIGVGKNYVIKGYGNSANGALYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKDGKYLIGIHVKDKY SKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEIGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEE NSINWKPTVGGKYLIGIHVKDKYSIDTLDDFIYENYTILSDNARLEKLEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILGTSFM >Mature_967_residues MKDKKYYLVPKIISIFFMLFLFSIKAQAVSNISINLKTPTDQNIINSSELNIDGEIKSILKLKSVYLYIDNENIGQANLD ELEFKENTYKARLKYTKNISSLKNGIHNLRILAIDEQNNKEEKEISINIKRDQEKSETNKNTIQNNFVDTTVKMGSIEKS LTTVSNEAKLEKVEVSYNGNVVTNGEMGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGK YLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEMGVGKSYLIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGSVITNGEIGVGKNYVIKGYGNSANGALYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKDGKYLIGIHVKDKY SKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEIGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEE NSINWKPTVGGKYLIGIHVKDKYSIDTLDDFIYENYTILSDNARLEKLEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILGTSFM
Specific function: Unknown
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Secreted, cell wall, S-layer (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 3 SLH (S-layer homology) domains [H]
Homologues:
Organism=Escherichia coli, GI1788776, Length=227, Percent_Identity=31.7180616740088, Blast_Score=98, Evalue=3e-21, Organism=Escherichia coli, GI87082163, Length=220, Percent_Identity=31.8181818181818, Blast_Score=98, Evalue=3e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002508 - InterPro: IPR001119 [H]
Pfam domain/function: PF01520 Amidase_3; PF00395 SLH [H]
EC number: 3.5.1.28 [C]
Molecular weight: Translated: 108771; Mature: 108771
Theoretical pI: Translated: 6.64; Mature: 6.64
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.3 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKDKKYYLVPKIISIFFMLFLFSIKAQAVSNISINLKTPTDQNIINSSELNIDGEIKSIL CCCCEEEEHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHH KLKSVYLYIDNENIGQANLDELEFKENTYKARLKYTKNISSLKNGIHNLRILAIDEQNNK EEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEEECCHHHHHCCCCEEEEEEEECCCCC EEKEISINIKRDQEKSETNKNTIQNNFVDTTVKMGSIEKSLTTVSNEAKLEKVEVSYNGN CCEEEEEEEECCCCHHHCCCHHHHCCCEEEEEEECCCHHHHHHCCCCCEEEEEEEEECCC VVTNGEMGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGK EEECCCCCCCCEEEEEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCCCCCCHHCCCC YLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEMGVGKSYLIKG EEEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEEEEEEEECCCEEECCCCCCCCEEEEEE YGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLD CCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCH DFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDL HHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECC STNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE CCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEE KVEVSYNGSVITNGEIGVGKNYVIKGYGNSANGALYQFWVKDLSTNSWTMIRDYGETNSF EEEEEECCCEEECCCCCCCCCEEEEECCCCCCCCEEEEEEHHCCCCCEEEEEECCCCCCC NYTPAKDGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEIG CCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEEEEEEEECCCEEECCCCC VGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEENSINWKPTVGGKYLIGIHVK CCCEEEEEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCCCEEEECCCCEEEEEEEEE DKYSIDTLDDFIYENYTILSDNARLEKLEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL CCCCCCHHHHHHHCCEEEEECCCEEEEEEEEECCEEEECCCCCCEEEEEECCCCCCCCEE YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSI EEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCEEE IGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVS EECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCEEEEEECCCCCC WPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKE CCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHH LVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG HHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHH ILGTSFM HCCCCCC >Mature Secondary Structure MKDKKYYLVPKIISIFFMLFLFSIKAQAVSNISINLKTPTDQNIINSSELNIDGEIKSIL CCCCEEEEHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHH KLKSVYLYIDNENIGQANLDELEFKENTYKARLKYTKNISSLKNGIHNLRILAIDEQNNK EEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEEECCHHHHHCCCCEEEEEEEECCCCC EEKEISINIKRDQEKSETNKNTIQNNFVDTTVKMGSIEKSLTTVSNEAKLEKVEVSYNGN CCEEEEEEEECCCCHHHCCCHHHHCCCEEEEEEECCCHHHHHHCCCCCEEEEEEEEECCC VVTNGEMGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGK EEECCCCCCCCEEEEEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCCCCCCHHCCCC YLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEMGVGKSYLIKG EEEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEEEEEEEECCCEEECCCCCCCCEEEEEE YGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLD CCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCH DFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDL HHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECC STNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE CCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEE KVEVSYNGSVITNGEIGVGKNYVIKGYGNSANGALYQFWVKDLSTNSWTMIRDYGETNSF EEEEEECCCEEECCCCCCCCCEEEEECCCCCCCCEEEEEEHHCCCCCEEEEEECCCCCCC NYTPAKDGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYNGNVVTNGEIG CCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEEEEEEEECCCEEECCCCC VGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEENSINWKPTVGGKYLIGIHVK CCCEEEEEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCCCEEEECCCCEEEEEEEEE DKYSIDTLDDFIYENYTILSDNARLEKLEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL CCCCCCHHHHHHHCCEEEEECCCEEEEEEEEECCEEEECCCCCCEEEEEECCCCCCCCEE YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSI EEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCEEE IGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVS EECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCEEEEEECCCCCC WPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKE CCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHH LVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG HHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHH ILGTSFM HCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]
Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]
General reaction: Carboxylic acid amide hydrolysis [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10475962; 12004073 [H]