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The map label for this gene is purT [C]

Identifier: 170755835

GI number: 170755835

Start: 2885755

End: 2886900

Strand: Reverse

Name: purT [C]

Synonym: CLD_1856

Alternate gene names: 170755835

Gene position: 2886900-2885755 (Counterclockwise)

Preceding gene: 170756683

Following gene: 170755052

Centisome position: 72.93

GC content: 33.42

Gene sequence:

>1146_bases
ATGAAGAAATTAATGATTATAGGCTCAGGAGAGCTGCAGGTTCCATTAATCAAGATTTGCAAGAAAAATGGATATGAAGT
AATAGCTACAGATAGGGATGAAAATGCCAAAGGCTTTGCATATGCTGACAAATCCATAGTTGTTGATGGCACAGATAAAG
AGAAGATATTAGAGTATGCCATAAAGTGGAATATTGATGGAATTGTTACAACGGCTGAGATTACATTGTTTACAGTTGTA
TTTGTATGTGAAAAGCTAGGGTTGAAGGGGCCTTCAATGAAAGCGGCACAGATATCGAATGATAAATATTTGATGAGAAA
ATGCATGATTGAAAATGGCATTAAATCACCTAGGTTCTGGTTGGTTCATAACAATGATGAACTAAATGAGATTGAAAATG
AAATAAAATTTCCAGTGGTAATCAAACCGGTCGATGTTTCAGGCAGCATTGGCGTTATGAAGGCTGATAACATGGATGAG
ATAAAAGCAATATTTGACGATATATCGGTAAAAAGTAGAAGCAATAGGGTTATAATTGAAGAATTTATGGAGGGACCAGA
ATTTAGTGTTGAAACATTAAGTATGAATGGAATTCATAATATGATTGCGATAACAGAAAAAAAAGTTTCTGGATATCCCT
ACTTTGTAGAAGAAAGACATGTTATACCTGCTAATATCAACAATAAGCAAGAAAAAGAAATAAAGGACCTGGTTATCAGA
TTGTTGAATGCTTGTAAATTTGATAATTGCGCAGGGCATACAGAAGTAAAACTCACTGAAGACGGTCCCATGATTGTAGA
AACTGGTGCAAGAATAGGTGGAGACTATATTGCATCAGATTTAGTTCCGTTGGCAACGGGAATAAGCATGCATCAAAGCA
TAGCAAAAATAGCTTTGGGGGAAGAGATAAGCTTTGAAAAAAAGTTGAAAAAGTACGCGGGAATTCAGTATGTTGTTCCA
GACAATTTTCAATCAATAGTTGAAAAACAAGACAAAATTACTAAAACAAAAGAATTTGTAAAATATGAAATGAATGATAA
TATTAAAAATGTTAAATTGCAAAGTTCACATGATAGATTGGGATATTATATTTTTGCAGCAGATACAAGGGAAGAACTAA
TGCATCAGTTTAAATTTTACAAGTAA

Upstream 100 bases:

>100_bases
CAATACTTTCAATACCCATTGACTGGAGATATGATAAAAAAGATATGCACTATATTTTAGAGCAATTAATCAGTATTATC
TAATTTTAGGAGTAGAATTT

Downstream 100 bases:

>100_bases
ATAATAAGGATGGAAGTAGCAATAAACGTACTTTTTGAAAAATTCACAGATTTAAATCTTCCTTATTATTATGAAATAAG
ATGTGATATTAGCAATATCT

Product: putative carbamoyl-phosphate synthase large chain

Products: ADP; phosphate; L-glutamate; carbamoyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKKLMIIGSGELQVPLIKICKKNGYEVIATDRDENAKGFAYADKSIVVDGTDKEKILEYAIKWNIDGIVTTAEITLFTVV
FVCEKLGLKGPSMKAAQISNDKYLMRKCMIENGIKSPRFWLVHNNDELNEIENEIKFPVVIKPVDVSGSIGVMKADNMDE
IKAIFDDISVKSRSNRVIIEEFMEGPEFSVETLSMNGIHNMIAITEKKVSGYPYFVEERHVIPANINNKQEKEIKDLVIR
LLNACKFDNCAGHTEVKLTEDGPMIVETGARIGGDYIASDLVPLATGISMHQSIAKIALGEEISFEKKLKKYAGIQYVVP
DNFQSIVEKQDKITKTKEFVKYEMNDNIKNVKLQSSHDRLGYYIFAADTREELMHQFKFYK

Sequences:

>Translated_381_residues
MKKLMIIGSGELQVPLIKICKKNGYEVIATDRDENAKGFAYADKSIVVDGTDKEKILEYAIKWNIDGIVTTAEITLFTVV
FVCEKLGLKGPSMKAAQISNDKYLMRKCMIENGIKSPRFWLVHNNDELNEIENEIKFPVVIKPVDVSGSIGVMKADNMDE
IKAIFDDISVKSRSNRVIIEEFMEGPEFSVETLSMNGIHNMIAITEKKVSGYPYFVEERHVIPANINNKQEKEIKDLVIR
LLNACKFDNCAGHTEVKLTEDGPMIVETGARIGGDYIASDLVPLATGISMHQSIAKIALGEEISFEKKLKKYAGIQYVVP
DNFQSIVEKQDKITKTKEFVKYEMNDNIKNVKLQSSHDRLGYYIFAADTREELMHQFKFYK
>Mature_381_residues
MKKLMIIGSGELQVPLIKICKKNGYEVIATDRDENAKGFAYADKSIVVDGTDKEKILEYAIKWNIDGIVTTAEITLFTVV
FVCEKLGLKGPSMKAAQISNDKYLMRKCMIENGIKSPRFWLVHNNDELNEIENEIKFPVVIKPVDVSGSIGVMKADNMDE
IKAIFDDISVKSRSNRVIIEEFMEGPEFSVETLSMNGIHNMIAITEKKVSGYPYFVEERHVIPANINNKQEKEIKDLVIR
LLNACKFDNCAGHTEVKLTEDGPMIVETGARIGGDYIASDLVPLATGISMHQSIAKIALGEEISFEKKLKKYAGIQYVVP
DNFQSIVEKQDKITKTKEFVKYEMNDNIKNVKLQSSHDRLGYYIFAADTREELMHQFKFYK

Specific function: Catalyzes Two Reactions:The First One Is The Production Of Beta-Formyl Glycinamide Ribonucleotide (Gar) From Formate, ATP And Beta Gar; The Second, A Side Reaction, Is The Production Of Acetyl Phosphate And ADP From Acetate And ATP. [C]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479 [H]

Pfam domain/function: PF02786 CPSase_L_D2 [H]

EC number: 6.3.5.5

Molecular weight: Translated: 43144; Mature: 43144

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLMIIGSGELQVPLIKICKKNGYEVIATDRDENAKGFAYADKSIVVDGTDKEKILEYA
CCEEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEECCEEEEECCCHHHHHHHH
IKWNIDGIVTTAEITLFTVVFVCEKLGLKGPSMKAAQISNDKYLMRKCMIENGIKSPRFW
HEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEE
LVHNNDELNEIENEIKFPVVIKPVDVSGSIGVMKADNMDEIKAIFDDISVKSRSNRVIIE
EEECCCHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEHH
EFMEGPEFSVETLSMNGIHNMIAITEKKVSGYPYFVEERHVIPANINNKQEKEIKDLVIR
HHHCCCCCEEEEEEECCCCCEEEEEEHHCCCCCEEEECCEEEECCCCCHHHHHHHHHHHH
LLNACKFDNCAGHTEVKLTEDGPMIVETGARIGGDYIASDLVPLATGISMHQSIAKIALG
HHHHCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCC
EEISFEKKLKKYAGIQYVVPDNFQSIVEKQDKITKTKEFVKYEMNDNIKNVKLQSSHDRL
CCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCE
GYYIFAADTREELMHQFKFYK
EEEEEEECCHHHHHHHHCCCC
>Mature Secondary Structure
MKKLMIIGSGELQVPLIKICKKNGYEVIATDRDENAKGFAYADKSIVVDGTDKEKILEYA
CCEEEEEECCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEECCEEEEECCCHHHHHHHH
IKWNIDGIVTTAEITLFTVVFVCEKLGLKGPSMKAAQISNDKYLMRKCMIENGIKSPRFW
HEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEE
LVHNNDELNEIENEIKFPVVIKPVDVSGSIGVMKADNMDEIKAIFDDISVKSRSNRVIIE
EEECCCHHHHHHHHCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEHH
EFMEGPEFSVETLSMNGIHNMIAITEKKVSGYPYFVEERHVIPANINNKQEKEIKDLVIR
HHHCCCCCEEEEEEECCCCCEEEEEEHHCCCCCEEEECCEEEECCCCCHHHHHHHHHHHH
LLNACKFDNCAGHTEVKLTEDGPMIVETGARIGGDYIASDLVPLATGISMHQSIAKIALG
HHHHCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCC
EEISFEKKLKKYAGIQYVVPDNFQSIVEKQDKITKTKEFVKYEMNDNIKNVKLQSSHDRL
CCCCHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCE
GYYIFAADTREELMHQFKFYK
EEEEEEECCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine; CO2; H2O

Specific reaction: 2 ATP + L-glutamine + CO2 + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]