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The map label for this gene is queD [H]

Identifier: 170755668

GI number: 170755668

Start: 938745

End: 939098

Strand: Direct

Name: queD [H]

Synonym: CLD_3745

Alternate gene names: 170755668

Gene position: 938745-939098 (Clockwise)

Preceding gene: 170754838

Following gene: 170756530

Centisome position: 23.72

GC content: 30.23

Gene sequence:

>354_bases
ATGATACTTATTAAAAAATTCAAATTTGATGCAGCACATAATCTAATACATTACCATGGAAAATGTGAAAGACTTCATGG
CCATACTTATGGACTAGTAATAAAGATTTCAGGTGAAAGGGATAAGGAAGATATGGTAATAGATTTTACAGAGCTTAAAG
CCATAGTAAAAGAGAATGTATTAGATATATTAGATCATGCTTATATAAATGACATAATAGAGCAACCAACAGCAGAAAAT
ATAGCAGTGTGGATATGGGACAAACTTTATACAAAATTAGAAAGAGATAATTGTAGTTTATATGAAATAGAAGTTTGGGA
AACAGAAACTAGTGGCGTGGTTTACAACGGATAA

Upstream 100 bases:

>100_bases
ACAAATATAATTATATATAATGTTGTTTTATTAATATAATGTACTTTTGATATACTAATATATATCGAATGTATTTATAT
AGAGGAAAGGGAGTTATACT

Downstream 100 bases:

>100_bases
TAATAAAGAATTTATGAGAAATAATTAAAGTAAATTTCAGTTTGAGATAGTTCAATGTTTATATAGCTTAACAGAATGGT
TATAAAGAAAAGGGGCGTTA

Product: putative 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MILIKKFKFDAAHNLIHYHGKCERLHGHTYGLVIKISGERDKEDMVIDFTELKAIVKENVLDILDHAYINDIIEQPTAEN
IAVWIWDKLYTKLERDNCSLYEIEVWETETSGVVYNG

Sequences:

>Translated_117_residues
MILIKKFKFDAAHNLIHYHGKCERLHGHTYGLVIKISGERDKEDMVIDFTELKAIVKENVLDILDHAYINDIIEQPTAEN
IAVWIWDKLYTKLERDNCSLYEIEVWETETSGVVYNG
>Mature_117_residues
MILIKKFKFDAAHNLIHYHGKCERLHGHTYGLVIKISGERDKEDMVIDFTELKAIVKENVLDILDHAYINDIIEQPTAEN
IAVWIWDKLYTKLERDNCSLYEIEVWETETSGVVYNG

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Homo sapiens, GI4506331, Length=133, Percent_Identity=33.0827067669173, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789124, Length=120, Percent_Identity=43.3333333333333, Blast_Score=84, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19550074, Length=134, Percent_Identity=30.5970149253731, Blast_Score=62, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24585378, Length=134, Percent_Identity=30.5970149253731, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 13680; Mature: 13680

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIKKFKFDAAHNLIHYHGKCERLHGHTYGLVIKISGERDKEDMVIDFTELKAIVKENV
CEEEEEECCCCCCCEEEECCEEEEECCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHH
LDILDHAYINDIIEQPTAENIAVWIWDKLYTKLERDNCSLYEIEVWETETSGVVYNG
HHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEEEEEECCCCEEECC
>Mature Secondary Structure
MILIKKFKFDAAHNLIHYHGKCERLHGHTYGLVIKISGERDKEDMVIDFTELKAIVKENV
CEEEEEECCCCCCCEEEECCEEEEECCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHH
LDILDHAYINDIIEQPTAENIAVWIWDKLYTKLERDNCSLYEIEVWETETSGVVYNG
HHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHCCCCCCEEEEEEEEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]