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The map label for this gene is glyS

Identifier: 170754288

GI number: 170754288

Start: 3830028

End: 3831419

Strand: Reverse

Name: glyS

Synonym: CLD_0973

Alternate gene names: 170754288

Gene position: 3831419-3830028 (Counterclockwise)

Preceding gene: 170757532

Following gene: 170755247

Centisome position: 96.8

GC content: 30.96

Gene sequence:

>1392_bases
ATGGCTTTAAAAAAAACTATGGATAAAGTGGTTGCTTTAAGTAAAAATAGAGGATTTGTATTTCCGGGATCTGATATATA
TGGAGGATTAGCAAATTCATGGGATTATGGTCCGCTAGGAGCAGAACTAAAAAATAATGTAAAAAAAGCTTGGTGGAAAA
AGTTTGTTCAAGAAAGTCCATATAATGTTGGATTAGATGCGGCTATTTTAATGAATAACCAAGTTTGGGTAGCATCTGGA
CATGTAGGAGGATTTTCTGATCCTTTAATGGATTGTAAAGAATGTAAAGCAAGGTTTAGAGCAGACAAACTAGTAGAAGA
GCATATGACAGAACAAGGCGTCGAAAAAGCTAGTGCAGATGGTTGGTCAAATGAAAAATTAAAACAATATATAGATGATA
ATGGTATAGAATGTCCAAAATGTAAAGCTAAAAACTTTACGGATATAAGAAAATTCAATTTAATGTTCAAAACATTCCAA
GGAGTAACAGAAGATGCTAAGTCAGAAATATATTTAAGACCAGAAACAGCTCAAGGTATATTTGTTAATTTTAAAAATGT
TCAAAGAACATCTAGAAAAAAAGTTCCATTTGGTATTGCGCAAATAGGAAAATCTTTTAGAAATGAAATAACACCAGGAA
ACTTTACATTTAGAACTAGAGAATTTGAACAAATGGAGTTAGAGTTTTTCTGTAAACCAGGAACAGATTTAGAATGGTTT
AATTATTGGAAAGATTATTGCTGGAATTTCTTGATAAATTTAGGTATAAACAAAGAAAATATAAGATTTAGAGATCATAG
TCAAGAAGAATTATCTCACTATAGTAATGCTACATCAGATATAGAATTTTTATTTCCATTTGGTTGGGGTGAGTTATGGG
GCGTTGCAGATAGAACAGATTTCGACTTGAAAAAGCATATGGAACATTCAGGAGAAGATCTAAACTATTTAGATCCAGCA
ACTAAAGAAAGATATGTGCCATATTGTATAGAACCATCTTTAGGAGCGGATAGGGTTGTTTTAGCATTTTTAGTAGATGC
TTATGATGAAGAAGAACTAGAAGGTGGAGATGTGAGAAATGTATTACATTTACACCCAGCTTTGGCTCCATTTAAAGCAG
CAGTATTGCCACTTAGTAAAAAACTTTCTGATAAATCACAAGAAGTTTACAGTATGTTAAGCAAAAAGTTTAATATAGAT
TATGATGAGGCAGGCAGCATAGGAAAAAGATATAGAAGAGAAGATGAAATAGGAACTCCTTATTGTATAACAGTAGATTT
TGATACATTAGAAGATAATACAGTTACTATAAGAGACAGAGATACTATGAATCAAATAAGAGTTAGTATAAATGAACTAG
AAAAATTTATAGAAGACAAATTAGAATTTTAA

Upstream 100 bases:

>100_bases
ATAGCCAAAAAGGGTGGAACCGCGATTAAGTCGTCCCTTTGTTGCTATAGCCTTTTTTATATTAAAAATTTTATGAAAAA
ATATTAGGGAGGTACTTTTT

Downstream 100 bases:

>100_bases
AATAAAACCTATTAATAATAGAATTTAATAACATATAAATTTTATATTTAAATTAAAAAGCTATGGATTATAGAGTAATA
TCCTATAATTCATAGTTTTA

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MALKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGAELKNNVKKAWWKKFVQESPYNVGLDAAILMNNQVWVASG
HVGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASADGWSNEKLKQYIDDNGIECPKCKAKNFTDIRKFNLMFKTFQ
GVTEDAKSEIYLRPETAQGIFVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWF
NYWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTDFDLKKHMEHSGEDLNYLDPA
TKERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRNVLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNID
YDEAGSIGKRYRREDEIGTPYCITVDFDTLEDNTVTIRDRDTMNQIRVSINELEKFIEDKLEF

Sequences:

>Translated_463_residues
MALKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGAELKNNVKKAWWKKFVQESPYNVGLDAAILMNNQVWVASG
HVGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASADGWSNEKLKQYIDDNGIECPKCKAKNFTDIRKFNLMFKTFQ
GVTEDAKSEIYLRPETAQGIFVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWF
NYWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTDFDLKKHMEHSGEDLNYLDPA
TKERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRNVLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNID
YDEAGSIGKRYRREDEIGTPYCITVDFDTLEDNTVTIRDRDTMNQIRVSINELEKFIEDKLEF
>Mature_462_residues
ALKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGAELKNNVKKAWWKKFVQESPYNVGLDAAILMNNQVWVASGH
VGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASADGWSNEKLKQYIDDNGIECPKCKAKNFTDIRKFNLMFKTFQG
VTEDAKSEIYLRPETAQGIFVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWFN
YWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTDFDLKKHMEHSGEDLNYLDPAT
KERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRNVLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNIDY
DEAGSIGKRYRREDEIGTPYCITVDFDTLEDNTVTIRDRDTMNQIRVSINELEKFIEDKLEF

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=382, Percent_Identity=32.1989528795811, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI70887790, Length=311, Percent_Identity=23.7942122186495, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI32564541, Length=353, Percent_Identity=33.7110481586402, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17554996, Length=353, Percent_Identity=33.7110481586402, Blast_Score=169, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6325338, Length=365, Percent_Identity=30.958904109589, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319597, Length=404, Percent_Identity=28.4653465346535, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21357965, Length=363, Percent_Identity=32.7823691460055, Blast_Score=178, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24664462, Length=363, Percent_Identity=32.7823691460055, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 53409; Mature: 53278

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGAELKNNVKKAWWKKFVQESP
CCHHHHHHHHHHHCCCCCEECCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
YNVGLDAAILMNNQVWVASGHVGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASAD
CCCCCCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GWSNEKLKQYIDDNGIECPKCKAKNFTDIRKFNLMFKTFQGVTEDAKSEIYLRPETAQGI
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEE
FVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWF
EEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHEEEEEEECCCCCCHHH
NYWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTD
HHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCC
FDLKKHMEHSGEDLNYLDPATKERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRN
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCHHHCCCCCHHH
VLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNIDYDEAGSIGKRYRREDEIGTP
HHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCCCC
YCITVDFDTLEDNTVTIRDRDTMNQIRVSINELEKFIEDKLEF
EEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALKKTMDKVVALSKNRGFVFPGSDIYGGLANSWDYGPLGAELKNNVKKAWWKKFVQESP
CHHHHHHHHHHHCCCCCEECCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
YNVGLDAAILMNNQVWVASGHVGGFSDPLMDCKECKARFRADKLVEEHMTEQGVEKASAD
CCCCCCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GWSNEKLKQYIDDNGIECPKCKAKNFTDIRKFNLMFKTFQGVTEDAKSEIYLRPETAQGI
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEE
FVNFKNVQRTSRKKVPFGIAQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGTDLEWF
EEEEHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHEEEEEEECCCCCCHHH
NYWKDYCWNFLINLGINKENIRFRDHSQEELSHYSNATSDIEFLFPFGWGELWGVADRTD
HHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHCCCCCCEEEEEECCCHHHCCCCCCCC
FDLKKHMEHSGEDLNYLDPATKERYVPYCIEPSLGADRVVLAFLVDAYDEEELEGGDVRN
HHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCHHHCCCCCHHH
VLHLHPALAPFKAAVLPLSKKLSDKSQEVYSMLSKKFNIDYDEAGSIGKRYRREDEIGTP
HHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCCCC
YCITVDFDTLEDNTVTIRDRDTMNQIRVSINELEKFIEDKLEF
EEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA