Click here to switch to the map view.

The map label for this gene is cloSI

Identifier: 170754979

GI number: 170754979

Start: 2049229

End: 2050803

Strand: Reverse

Name: cloSI

Synonym: CLD_2707

Alternate gene names: 170754979

Gene position: 2050803-2049229 (Counterclockwise)

Preceding gene: 170755766

Following gene: 170756568

Centisome position: 51.81

GC content: 29.84

Gene sequence:

>1575_bases
ATGTTAAAAAACAAGTTATCTATTCTTTTAACTTCTACTTTGGTGGCTGCTTCTCTTTTTAGTTTTAAACCTGTATATGC
GGATTCAGTGAATAATTCTAATAAAACTAATCTAAATAATGTTAGGCAGGAAATACAAAAAGCAGAAAATAATAATCAAA
AGGTAACAATTATGTATTATTGTGATGCTGATAATAATTTAGAATCATCTTTATTAAGTGACATTGAAGAAATGAAGAAA
GGATATGTTAACAATCCTAATCTAAATTTGGTGACACTTATAGATAGATCATCAAGATATACTAGTGACAAAACAGTTTT
TGGAGAAGATTTTGAAGATGCCCGCTTATATAAGATTGAACATAATAAAACTAAAAGATTAGATGGTGGAAAGGAATTTC
CTGAAATAACTCTTAATAGTAATTATGAAGCAAACATGGGAGATGCAGATACTCTTAAAAAATTCATTAATTATTGTAAA
GCTAATTATAAAGCTGATAAATATGTTTTAATAATGGCTAACCATGGTGGCGGAGCAAAAGAAAAGCTTAAAAATAATCA
AGATGTAAACAGAGCAATTTGTTGGGATGATAGTCATTATGATGGAAATAGTCCAGATTGTCTTTATATGGGAGAAATTT
CAGATACTTTAACACAGGAGCAATCTGTTGATGTACTTGCTTTTGATGCATGCTTAATGGGAACAGCAGAGGTTGCATAT
CAATTTAGACCAGGTAATGGAGGATTTTCAGCAGATGGAATAGTTGCTTCAAGTCCTGTAGTTTGGGGGCCAGGATTCCA
ATATGATAATATTTTAAGTAGATTAAAATCTGGTGGAGGAAGTAGCAATGAAGATGATTTAACTTTAGGTGGTAAAGAGA
AAAACTTTGATCCTGCAACTATTACTAATGAACAATTAGGAGCATTATTTGTAGAAGAACAAAGAGATTCTACTCATGCT
AGAGGAAGCTATGATCAACATTTGAGCTTTTATGATGCTACTAAAGTAGAAGATGTAAAAAGATCAATAGATAATTTAGC
TATTAATCTAAGCAATGAAAATAAAAAAGATGAAATTGAAAAATTAAGAGGAAGTAGAAATAATACAAATTTAATGCACT
ATTTTGATGAATATAATGAACAAGAATGGATTGAATATCCTTATTTTGATATATATGATTTATGCGAAAAAATAAATGAA
AGTAATAATTTTAGCAATGAAACTAGAACATTGGCTTCAATCTGCATGGATAAAATAGATGAAATGGTAGTCTATTCTTT
TGGTGGCCCTAGCAAAGAATTTAAAGAAGGTAAAAATGGATTAAGTATATTCCTACCAGATGGAGATAAAAAATATTCAA
GTTATTATAATTCAATTCCTCATTGGACTATTCAAAGTTGGTATAATTCAATAGATACAGTTCAAAATGGATTACCTCCT
TATGGAAAATTAACTTGGTGCCAAGATGGACAAGATCCAAGAGTAAATAAAGTTGGAAATTGGTTCGAACTTTTAGATTC
TTGGTTTGACAAAACTAATGGAGCAGATGGAGGGGTTAACCATTATCAATGGTAA

Upstream 100 bases:

>100_bases
GTACATATCGTGCACATACATGGGGTGTTTCTATTAATATTAAATACATTATAATTCTGCATAATAAATCAAAAATATAA
AATTTGGGGGTATGTATATT

Downstream 100 bases:

>100_bases
AACACATTTTTAGCTTAATTTATTATTAAAAATACTGTCTAAAAATAGAAGTTATGCTTTCTATTTTTAGGCAGTATTTT
AATTATATTATTTTTCATTC

Product: clostripain

Products: NA

Alternate protein names: Clostridiopeptidase B [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MLKNKLSILLTSTLVAASLFSFKPVYADSVNNSNKTNLNNVRQEIQKAENNNQKVTIMYYCDADNNLESSLLSDIEEMKK
GYVNNPNLNLVTLIDRSSRYTSDKTVFGEDFEDARLYKIEHNKTKRLDGGKEFPEITLNSNYEANMGDADTLKKFINYCK
ANYKADKYVLIMANHGGGAKEKLKNNQDVNRAICWDDSHYDGNSPDCLYMGEISDTLTQEQSVDVLAFDACLMGTAEVAY
QFRPGNGGFSADGIVASSPVVWGPGFQYDNILSRLKSGGGSSNEDDLTLGGKEKNFDPATITNEQLGALFVEEQRDSTHA
RGSYDQHLSFYDATKVEDVKRSIDNLAINLSNENKKDEIEKLRGSRNNTNLMHYFDEYNEQEWIEYPYFDIYDLCEKINE
SNNFSNETRTLASICMDKIDEMVVYSFGGPSKEFKEGKNGLSIFLPDGDKKYSSYYNSIPHWTIQSWYNSIDTVQNGLPP
YGKLTWCQDGQDPRVNKVGNWFELLDSWFDKTNGADGGVNHYQW

Sequences:

>Translated_524_residues
MLKNKLSILLTSTLVAASLFSFKPVYADSVNNSNKTNLNNVRQEIQKAENNNQKVTIMYYCDADNNLESSLLSDIEEMKK
GYVNNPNLNLVTLIDRSSRYTSDKTVFGEDFEDARLYKIEHNKTKRLDGGKEFPEITLNSNYEANMGDADTLKKFINYCK
ANYKADKYVLIMANHGGGAKEKLKNNQDVNRAICWDDSHYDGNSPDCLYMGEISDTLTQEQSVDVLAFDACLMGTAEVAY
QFRPGNGGFSADGIVASSPVVWGPGFQYDNILSRLKSGGGSSNEDDLTLGGKEKNFDPATITNEQLGALFVEEQRDSTHA
RGSYDQHLSFYDATKVEDVKRSIDNLAINLSNENKKDEIEKLRGSRNNTNLMHYFDEYNEQEWIEYPYFDIYDLCEKINE
SNNFSNETRTLASICMDKIDEMVVYSFGGPSKEFKEGKNGLSIFLPDGDKKYSSYYNSIPHWTIQSWYNSIDTVQNGLPP
YGKLTWCQDGQDPRVNKVGNWFELLDSWFDKTNGADGGVNHYQW
>Mature_524_residues
MLKNKLSILLTSTLVAASLFSFKPVYADSVNNSNKTNLNNVRQEIQKAENNNQKVTIMYYCDADNNLESSLLSDIEEMKK
GYVNNPNLNLVTLIDRSSRYTSDKTVFGEDFEDARLYKIEHNKTKRLDGGKEFPEITLNSNYEANMGDADTLKKFINYCK
ANYKADKYVLIMANHGGGAKEKLKNNQDVNRAICWDDSHYDGNSPDCLYMGEISDTLTQEQSVDVLAFDACLMGTAEVAY
QFRPGNGGFSADGIVASSPVVWGPGFQYDNILSRLKSGGGSSNEDDLTLGGKEKNFDPATITNEQLGALFVEEQRDSTHA
RGSYDQHLSFYDATKVEDVKRSIDNLAINLSNENKKDEIEKLRGSRNNTNLMHYFDEYNEQEWIEYPYFDIYDLCEKINE
SNNFSNETRTLASICMDKIDEMVVYSFGGPSKEFKEGKNGLSIFLPDGDKKYSSYYNSIPHWTIQSWYNSIDTVQNGLPP
YGKLTWCQDGQDPRVNKVGNWFELLDSWFDKTNGADGGVNHYQW

Specific function: Cysteine endopeptidase with strict specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014173
- InterPro:   IPR005077 [H]

Pfam domain/function: PF03415 Peptidase_C11 [H]

EC number: =3.4.22.8 [H]

Molecular weight: Translated: 59398; Mature: 59398

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNKLSILLTSTLVAASLFSFKPVYADSVNNSNKTNLNNVRQEIQKAENNNQKVTIMYY
CCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
CDADNNLESSLLSDIEEMKKGYVNNPNLNLVTLIDRSSRYTSDKTVFGEDFEDARLYKIE
ECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEE
HNKTKRLDGGKEFPEITLNSNYEANMGDADTLKKFINYCKANYKADKYVLIMANHGGGAK
CCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
EKLKNNQDVNRAICWDDSHYDGNSPDCLYMGEISDTLTQEQSVDVLAFDACLMGTAEVAY
HHHCCCCCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHCCCCEEEEHHHHHCCCHHEE
QFRPGNGGFSADGIVASSPVVWGPGFQYDNILSRLKSGGGSSNEDDLTLGGKEKNFDPAT
EEECCCCCCCCCCEEECCCEEECCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCC
ITNEQLGALFVEEQRDSTHARGSYDQHLSFYDATKVEDVKRSIDNLAINLSNENKKDEIE
CCCHHHCEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHH
KLRGSRNNTNLMHYFDEYNEQEWIEYPYFDIYDLCEKINESNNFSNETRTLASICMDKID
HHHCCCCCCCEEEHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
EMVVYSFGGPSKEFKEGKNGLSIFLPDGDKKYSSYYNSIPHWTIQSWYNSIDTVQNGLPP
HEEEEECCCCCHHHHCCCCCCEEEECCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCC
YGKLTWCQDGQDPRVNKVGNWFELLDSWFDKTNGADGGVNHYQW
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLKNKLSILLTSTLVAASLFSFKPVYADSVNNSNKTNLNNVRQEIQKAENNNQKVTIMYY
CCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
CDADNNLESSLLSDIEEMKKGYVNNPNLNLVTLIDRSSRYTSDKTVFGEDFEDARLYKIE
ECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEE
HNKTKRLDGGKEFPEITLNSNYEANMGDADTLKKFINYCKANYKADKYVLIMANHGGGAK
CCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
EKLKNNQDVNRAICWDDSHYDGNSPDCLYMGEISDTLTQEQSVDVLAFDACLMGTAEVAY
HHHCCCCCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHCCCCEEEEHHHHHCCCHHEE
QFRPGNGGFSADGIVASSPVVWGPGFQYDNILSRLKSGGGSSNEDDLTLGGKEKNFDPAT
EEECCCCCCCCCCEEECCCEEECCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCC
ITNEQLGALFVEEQRDSTHARGSYDQHLSFYDATKVEDVKRSIDNLAINLSNENKKDEIE
CCCHHHCEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHH
KLRGSRNNTNLMHYFDEYNEQEWIEYPYFDIYDLCEKINESNNFSNETRTLASICMDKID
HHHCCCCCCCEEEHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
EMVVYSFGGPSKEFKEGKNGLSIFLPDGDKKYSSYYNSIPHWTIQSWYNSIDTVQNGLPP
HEEEEECCCCCHHHHCCCCCCEEEECCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCC
YGKLTWCQDGQDPRVNKVGNWFELLDSWFDKTNGADGGVNHYQW
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8341259; 6391922; 6337850 [H]