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The map label for this gene is thlA [H]

Identifier: 170754683

GI number: 170754683

Start: 3477239

End: 3478417

Strand: Reverse

Name: thlA [H]

Synonym: CLD_1331

Alternate gene names: 170754683

Gene position: 3478417-3477239 (Counterclockwise)

Preceding gene: 170755442

Following gene: 170755246

Centisome position: 87.88

GC content: 34.52

Gene sequence:

>1179_bases
ATGAAAAATGTTGTTATTGCTAGTGCTGTAAGAACACCTATAGGAAAATTTGGAGGAACATTAAAAAGTGTTTCAGCTAT
AGAATTAGGAAGTATTGTTATTAAAGAAGCTTTAAAAAGAGCTAATGTAAAACCAGAAGATGTTGATGAGGTTATAATGG
GTAATGTACTTCAAGCAGGTCTTGGTCAAAACTCAACAAGACAATCTGCAGTAGCGGCAGGTATACCAGTAGAAGTACCA
AGCTTTACAATAAATAAGGTTTGTGGTTCAGGACTTAGAGCAGTAAGTTTAGCAACTCAATTAATAAAACTCGGAGATGA
CGATATAGTAGTAGCAGGTGGTACAGAAAATATGTCTGCAGCTCCATATCTTTTAGAAAAAGCAAGATGGGGACAGAGAA
TGGGCGATGGAAAAATTGTAGACGAGATGATAAGAGATGGACTATGGGAATCTTTTAATGACTATCATATGGGAATTACG
GCTGAAAATATAGCAGAGCAATGGGGAATAACAAGAGAAGAGCAAGACGAATTTTCAGCTAAATCACAACAAAAAGCAGA
GAAAGCTGTAAAAGAAGGAAGATTTAAAGAGGAAATAGTACCAGTAGTTATTAAAAGTAGAAAAGGCGAAGTAGTATTTG
ATACTGATGAATTTCCTAGATTTGGTGCTACTAAAGAGAGCCTTTCTAAATTAAAACCAGCTTTCAAAAAAGATGGAACA
GTTACAGCTGGTAATGCTTCAGGTATAAATGATGGAGCAGCTGCTTTAGTTATTATGAGTGAAGAAAAGGCAAATGAATT
AGGAATTAAACCTTTAGCTAAAATAGTTGCTTATGGTACAAGTGGAGTAGATCCAAAGATAATGGGGTATGGACCATTTT
ATGCAACTAAAAAAACTTTAGAAAAAGCCAACCTAAAAATAGCAGATATGGATTTAATAGAAGCAAATGAAGCTTTTGCG
GCTCAAAGTTTAGCAGTAGCTAAAGATCTAGGTTTTGATATGGATAAAGTAAATGTAAATGGTGGAGCTATAGCTTTAGG
ACATCCAATTGGATGCTCAGGGGCTAGAATATTAACTACTTTATTATATGAAATGGAAAAAAGAAATTCAAAACATGGCT
TAGCTACCTTATGTATAGGTGGTGGAATGGGAACAGCTATTATTGTTGAAAGATATTAA

Upstream 100 bases:

>100_bases
TCCATTATTAATAAAAATGGTTAGAGGAAATTTATTAGGAAGAAAAACAGGAAAAGGATTTTATGAATATAAATAATAAT
TAAATTTGGGGGTATGGAAA

Downstream 100 bases:

>100_bases
TATATTTTTATTAAAGATATATAATTACTAGCTTACTAACATAATACAATTTATAAGATAACATTAACATTGCATCATTA
AAATAGATTTATTTTAATGA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKNVVIASAVRTPIGKFGGTLKSVSAIELGSIVIKEALKRANVKPEDVDEVIMGNVLQAGLGQNSTRQSAVAAGIPVEVP
SFTINKVCGSGLRAVSLATQLIKLGDDDIVVAGGTENMSAAPYLLEKARWGQRMGDGKIVDEMIRDGLWESFNDYHMGIT
AENIAEQWGITREEQDEFSAKSQQKAEKAVKEGRFKEEIVPVVIKSRKGEVVFDTDEFPRFGATKESLSKLKPAFKKDGT
VTAGNASGINDGAAALVIMSEEKANELGIKPLAKIVAYGTSGVDPKIMGYGPFYATKKTLEKANLKIADMDLIEANEAFA
AQSLAVAKDLGFDMDKVNVNGGAIALGHPIGCSGARILTTLLYEMEKRNSKHGLATLCIGGGMGTAIIVERY

Sequences:

>Translated_392_residues
MKNVVIASAVRTPIGKFGGTLKSVSAIELGSIVIKEALKRANVKPEDVDEVIMGNVLQAGLGQNSTRQSAVAAGIPVEVP
SFTINKVCGSGLRAVSLATQLIKLGDDDIVVAGGTENMSAAPYLLEKARWGQRMGDGKIVDEMIRDGLWESFNDYHMGIT
AENIAEQWGITREEQDEFSAKSQQKAEKAVKEGRFKEEIVPVVIKSRKGEVVFDTDEFPRFGATKESLSKLKPAFKKDGT
VTAGNASGINDGAAALVIMSEEKANELGIKPLAKIVAYGTSGVDPKIMGYGPFYATKKTLEKANLKIADMDLIEANEAFA
AQSLAVAKDLGFDMDKVNVNGGAIALGHPIGCSGARILTTLLYEMEKRNSKHGLATLCIGGGMGTAIIVERY
>Mature_392_residues
MKNVVIASAVRTPIGKFGGTLKSVSAIELGSIVIKEALKRANVKPEDVDEVIMGNVLQAGLGQNSTRQSAVAAGIPVEVP
SFTINKVCGSGLRAVSLATQLIKLGDDDIVVAGGTENMSAAPYLLEKARWGQRMGDGKIVDEMIRDGLWESFNDYHMGIT
AENIAEQWGITREEQDEFSAKSQQKAEKAVKEGRFKEEIVPVVIKSRKGEVVFDTDEFPRFGATKESLSKLKPAFKKDGT
VTAGNASGINDGAAALVIMSEEKANELGIKPLAKIVAYGTSGVDPKIMGYGPFYATKKTLEKANLKIADMDLIEANEAFA
AQSLAVAKDLGFDMDKVNVNGGAIALGHPIGCSGARILTTLLYEMEKRNSKHGLATLCIGGGMGTAIIVERY

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=54.3589743589744, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=45.8227848101266, Blast_Score=355, Evalue=3e-98,
Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=43.6548223350254, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=42.5, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI4504327, Length=430, Percent_Identity=34.4186046511628, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=32.3076923076923, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=61.4795918367347, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=56.7774936061381, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=47, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI48994986, Length=401, Percent_Identity=42.643391521197, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1788683, Length=412, Percent_Identity=32.2815533980583, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=49.616368286445, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI25147385, Length=391, Percent_Identity=41.4322250639386, Blast_Score=313, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=40.9669211195929, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=33.587786259542, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17551802, Length=427, Percent_Identity=32.0843091334895, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=45.25, Blast_Score=323, Evalue=4e-89,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=42.1717171717172, Blast_Score=269, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=55.2429667519182, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI17648125, Length=391, Percent_Identity=44.7570332480818, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24640423, Length=396, Percent_Identity=44.1919191919192, Blast_Score=314, Evalue=5e-86,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=32.3943661971831, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41770; Mature: 41770

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVVIASAVRTPIGKFGGTLKSVSAIELGSIVIKEALKRANVKPEDVDEVIMGNVLQAG
CCCEEEHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
LGQNSTRQSAVAAGIPVEVPSFTINKVCGSGLRAVSLATQLIKLGDDDIVVAGGTENMSA
CCCCCCHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
APYLLEKARWGQRMGDGKIVDEMIRDGLWESFNDYHMGITAENIAEQWGITREEQDEFSA
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHCCCCCHHHHHHH
KSQQKAEKAVKEGRFKEEIVPVVIKSRKGEVVFDTDEFPRFGATKESLSKLKPAFKKDGT
HHHHHHHHHHHCCCCHHHHHHHEEECCCCCEEECCCCCCCCCCCHHHHHHHCHHHHCCCC
VTAGNASGINDGAAALVIMSEEKANELGIKPLAKIVAYGTSGVDPKIMGYGPFYATKKTL
EEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHH
EKANLKIADMDLIEANEAFAAQSLAVAKDLGFDMDKVNVNGGAIALGHPIGCSGARILTT
HHCCCEEEECHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHHHHHH
LLYEMEKRNSKHGLATLCIGGGMGTAIIVERY
HHHHHHHCCCCCCEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MKNVVIASAVRTPIGKFGGTLKSVSAIELGSIVIKEALKRANVKPEDVDEVIMGNVLQAG
CCCEEEHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
LGQNSTRQSAVAAGIPVEVPSFTINKVCGSGLRAVSLATQLIKLGDDDIVVAGGTENMSA
CCCCCCHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
APYLLEKARWGQRMGDGKIVDEMIRDGLWESFNDYHMGITAENIAEQWGITREEQDEFSA
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHCCCCCHHHHHHH
KSQQKAEKAVKEGRFKEEIVPVVIKSRKGEVVFDTDEFPRFGATKESLSKLKPAFKKDGT
HHHHHHHHHHHCCCCHHHHHHHEEECCCCCEEECCCCCCCCCCCHHHHHHHCHHHHCCCC
VTAGNASGINDGAAALVIMSEEKANELGIKPLAKIVAYGTSGVDPKIMGYGPFYATKKTL
EEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHH
EKANLKIADMDLIEANEAFAAQSLAVAKDLGFDMDKVNVNGGAIALGHPIGCSGARILTT
HHCCCEEEECHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCHHHHHHH
LLYEMEKRNSKHGLATLCIGGGMGTAIIVERY
HHHHHHHCCCCCCEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]