| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is manR [H]
Identifier: 170754654
GI number: 170754654
Start: 2293152
End: 2295245
Strand: Reverse
Name: manR [H]
Synonym: CLD_2486
Alternate gene names: 170754654
Gene position: 2295245-2293152 (Counterclockwise)
Preceding gene: 170755440
Following gene: 170755077
Centisome position: 57.99
GC content: 28.41
Gene sequence:
>2094_bases GTGTTAAATAAAAGATGCTCAAATATCTTGCAGATGATAGTAAATAACGAAAGGCCTATAACTATAAAGGAAATTTCTAA AAAAGTCAATAAAAGTCCTAGAACTGTAAGATATGATTTAGATAAAATAGATGATTATTTAACTGAGATTGAATTTCCTA AACTTGAAAGAAAATCCAATTTAGGCATCAGCCTAGATTTAAAGGATGAAGAAATAAAAAAACTTTTTAAGATTATAGGC AAAATTAATAATTATGACTATGTTCTTTCTCAAAAAGAGAGAGTATTTTATATAATTTATGAGCTTTTAAATAAGAGTGA ATTTGTAACTATAAATATGCTTTCAGATAGGATGATGGTAAGCCGCAGTACTATAATAAATGATTTAATAGAGGTTAAAA AATGGCTATTAGAAAATAAAATAACCCTTGAAAGTTCAAAGGGACAGGGCATAAAAATATTGGGAAGAGAAAGGGATCTT AGACGGGCTGCAGTAAAGCTCTTTTTTCAGAGCATGGATTCTATAAACTTTTTCAATGTAACTACCCTTAAGCTTTTTAA TGATATAGATATAGACTTTATAAGAAATACTATTAAAATTGCAGAGGAGCAGATGGAAACTTCATTTTCAGATGATGCTT TTAATAATCTTGTTATTCATATAGCTATAGCCATTAAAAGAATAGAATTATCAAAGGATATAATAATGGATAGCGAAGAA CTTAAGAATCTAAGGAAAACTGCGGAATATGCCATTGCATCAGGTGTAGCTAAAATGCTTGAGGATAGATTTAAAATATT TATACCAGAGGACGAAATAGGCTATATAACAATTCATATTTTAGGTAGCAATACTTCTACCCTTGAAAATATTGTAAAGG ATGATTGGATTTATCTACATCTTATAGTATTTAAACTAATAGAAAATGTAGAAAACATAACAGGAATTAATTTTAGTAAG GATAATAAGCTTTTTGATAGTCTTGCTCAGCATATTCGTCCAGCTATATACAGGCTTAAACATGATATTAAAGTAAAAAA TCCATTGATAGAAGAAATAAAAGAAAAATATTCATATATATTTGAAAGTATAGAGGAAGGTGTAAAGTTCATAGAAGAGG ATATAGGTGATAGTGTAAATCAAGAGGAAATAGGATATCTTACACTACACTTTATGGCTTCCATAGAAAGAAGCAAAAAT AAAAAACATAGAAAACCAAATGTACTTATAGTATGCGCCACAGGCATAGGTACATCAAAGTTTATTTCTAATAAATTAAA ATCTATATTTGATATAAATATAATAGATACTATATCTAGCCATACTATGGAAAAGATCCTTAAATATAATAAAAACATAG ATTTAATTGTAACTACAATTCCATTAAAGGTTAAGGGGATAAAATGTATAGAAGTAAATACATTTTTAACAGAAAAAAAC ATCAGTGAATTAGGCTTATACTTTGCAAAATTTATTAGAGATAACAGTGAAGAATGCAATAGTTCCTGTAAATATGAAGA AAGAGATAAGGTGCAGGAGATTTTAAATATTGTAAAAGAAAATTGCACTATACATGATTATTATAAATTAAGAAATAAGC TAGCGCTATATTTGGATATAAAAGATCCTGCCCTTACTGAAAATCATAAACCTTCTTTAAAGGAACTACTAAAACCTGAT TTTATTAAATTAAATGAAGAAGCTAAAGACTGGGAAGATGCAGTAAGAAAAAGTGGGGAGATATTAATGAATAATGGATG TATAAAGGAATCCTATATAGATGCTATGGTAAACACAGTAAAGAATATGGGCCCTTATATTGTTATAGCTCCTGGAATAG CTATGCCCCATGCAGCACCAGAGGATGGAGTTCTAAAAACTGGTATAAGTATGCTAACCTTAAAAGATCCAATTTCCTTT GGAAATTCTGAACATGATCCAGTAAGTGTTATAATTTCAATATGTTCTATAGATAAAGTAAACCATATGAAGGCACTAAA AGAGTTAATGAGTATTATGGATCAGGAAGATTTCATCTCAAAGGTTAAAAGTATGAAAACTCCCTCTGAAATAGATCCCA TTTTATATAGTTAA
Upstream 100 bases:
>100_bases TATTTTATTGTGGATTATTACGCATTTTAAGTAATAGAGTAAATAAATTTTATTTAGCAATTATATTTTTATATTTAAAC ACTAAAGGGGATGATGCATC
Downstream 100 bases:
>100_bases ATTAAATAATAAAAACAAAAGCTCTCATAATCTGGGAGCTTTTGTTTTGTAATAATTTATAAGCTTATTTTTTATATTTA AGATAATAAGCTCTAATTGA
Product: PRD domain-containing protein
Products: NA
Alternate protein names: Putative phosphotransferase enzyme IIB component; Putative PTS system EIIB component; Putative phosphotransferase enzyme IIA component; Putative PTS system EIIA component [H]
Number of amino acids: Translated: 697; Mature: 697
Protein sequence:
>697_residues MLNKRCSNILQMIVNNERPITIKEISKKVNKSPRTVRYDLDKIDDYLTEIEFPKLERKSNLGISLDLKDEEIKKLFKIIG KINNYDYVLSQKERVFYIIYELLNKSEFVTINMLSDRMMVSRSTIINDLIEVKKWLLENKITLESSKGQGIKILGRERDL RRAAVKLFFQSMDSINFFNVTTLKLFNDIDIDFIRNTIKIAEEQMETSFSDDAFNNLVIHIAIAIKRIELSKDIIMDSEE LKNLRKTAEYAIASGVAKMLEDRFKIFIPEDEIGYITIHILGSNTSTLENIVKDDWIYLHLIVFKLIENVENITGINFSK DNKLFDSLAQHIRPAIYRLKHDIKVKNPLIEEIKEKYSYIFESIEEGVKFIEEDIGDSVNQEEIGYLTLHFMASIERSKN KKHRKPNVLIVCATGIGTSKFISNKLKSIFDINIIDTISSHTMEKILKYNKNIDLIVTTIPLKVKGIKCIEVNTFLTEKN ISELGLYFAKFIRDNSEECNSSCKYEERDKVQEILNIVKENCTIHDYYKLRNKLALYLDIKDPALTENHKPSLKELLKPD FIKLNEEAKDWEDAVRKSGEILMNNGCIKESYIDAMVNTVKNMGPYIVIAPGIAMPHAAPEDGVLKTGISMLTLKDPISF GNSEHDPVSVIISICSIDKVNHMKALKELMSIMDQEDFISKVKSMKTPSEIDPILYS
Sequences:
>Translated_697_residues MLNKRCSNILQMIVNNERPITIKEISKKVNKSPRTVRYDLDKIDDYLTEIEFPKLERKSNLGISLDLKDEEIKKLFKIIG KINNYDYVLSQKERVFYIIYELLNKSEFVTINMLSDRMMVSRSTIINDLIEVKKWLLENKITLESSKGQGIKILGRERDL RRAAVKLFFQSMDSINFFNVTTLKLFNDIDIDFIRNTIKIAEEQMETSFSDDAFNNLVIHIAIAIKRIELSKDIIMDSEE LKNLRKTAEYAIASGVAKMLEDRFKIFIPEDEIGYITIHILGSNTSTLENIVKDDWIYLHLIVFKLIENVENITGINFSK DNKLFDSLAQHIRPAIYRLKHDIKVKNPLIEEIKEKYSYIFESIEEGVKFIEEDIGDSVNQEEIGYLTLHFMASIERSKN KKHRKPNVLIVCATGIGTSKFISNKLKSIFDINIIDTISSHTMEKILKYNKNIDLIVTTIPLKVKGIKCIEVNTFLTEKN ISELGLYFAKFIRDNSEECNSSCKYEERDKVQEILNIVKENCTIHDYYKLRNKLALYLDIKDPALTENHKPSLKELLKPD FIKLNEEAKDWEDAVRKSGEILMNNGCIKESYIDAMVNTVKNMGPYIVIAPGIAMPHAAPEDGVLKTGISMLTLKDPISF GNSEHDPVSVIISICSIDKVNHMKALKELMSIMDQEDFISKVKSMKTPSEIDPILYS >Mature_697_residues MLNKRCSNILQMIVNNERPITIKEISKKVNKSPRTVRYDLDKIDDYLTEIEFPKLERKSNLGISLDLKDEEIKKLFKIIG KINNYDYVLSQKERVFYIIYELLNKSEFVTINMLSDRMMVSRSTIINDLIEVKKWLLENKITLESSKGQGIKILGRERDL RRAAVKLFFQSMDSINFFNVTTLKLFNDIDIDFIRNTIKIAEEQMETSFSDDAFNNLVIHIAIAIKRIELSKDIIMDSEE LKNLRKTAEYAIASGVAKMLEDRFKIFIPEDEIGYITIHILGSNTSTLENIVKDDWIYLHLIVFKLIENVENITGINFSK DNKLFDSLAQHIRPAIYRLKHDIKVKNPLIEEIKEKYSYIFESIEEGVKFIEEDIGDSVNQEEIGYLTLHFMASIERSKN KKHRKPNVLIVCATGIGTSKFISNKLKSIFDINIIDTISSHTMEKILKYNKNIDLIVTTIPLKVKGIKCIEVNTFLTEKN ISELGLYFAKFIRDNSEECNSSCKYEERDKVQEILNIVKENCTIHDYYKLRNKLALYLDIKDPALTENHKPSLKELLKPD FIKLNEEAKDWEDAVRKSGEILMNNGCIKESYIDAMVNTVKNMGPYIVIAPGIAMPHAAPEDGVLKTGISMLTLKDPISF GNSEHDPVSVIISICSIDKVNHMKALKELMSIMDQEDFISKVKSMKTPSEIDPILYS
Specific function: May regulate the expression of the mannose operon [H]
COG id: COG3711
COG function: function code K; Transcriptional antiterminator
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 PTS EIIB type-2 domain [H]
Homologues:
Organism=Escherichia coli, GI2367359, Length=131, Percent_Identity=34.3511450381679, Blast_Score=105, Evalue=8e-24, Organism=Escherichia coli, GI1790755, Length=127, Percent_Identity=31.496062992126, Blast_Score=92, Evalue=2e-19, Organism=Escherichia coli, GI1789302, Length=107, Percent_Identity=40.1869158878505, Blast_Score=86, Evalue=9e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013196 - InterPro: IPR007737 - InterPro: IPR011608 - InterPro: IPR016152 - InterPro: IPR002178 - InterPro: IPR013011 - InterPro: IPR011991 [H]
Pfam domain/function: PF08279 HTH_11; PF05043 Mga; PF00874 PRD; PF00359 PTS_EIIA_2 [H]
EC number: =2.7.1.69 [H]
Molecular weight: Translated: 80339; Mature: 80339
Theoretical pI: Translated: 6.59; Mature: 6.59
Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.9 %Met (Translated Protein) 4.0 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.9 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLNKRCSNILQMIVNNERPITIKEISKKVNKSPRTVRYDLDKIDDYLTEIEFPKLERKSN CCCHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCCCCC LGISLDLKDEEIKKLFKIIGKINNYDYVLSQKERVFYIIYELLNKSEFVTINMLSDRMMV CCEEEECCHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCEEEEEEHHHHHHH SRSTIINDLIEVKKWLLENKITLESSKGQGIKILGRERDLRRAAVKLFFQSMDSINFFNV HHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEE TTLKLFNDIDIDFIRNTIKIAEEQMETSFSDDAFNNLVIHIAIAIKRIELSKDIIMDSEE EEEEHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEHHHHHHHHHHHHHCCHHH LKNLRKTAEYAIASGVAKMLEDRFKIFIPEDEIGYITIHILGSNTSTLENIVKDDWIYLH HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHH LIVFKLIENVENITGINFSKDNKLFDSLAQHIRPAIYRLKHDIKVKNPLIEEIKEKYSYI HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH FESIEEGVKFIEEDIGDSVNQEEIGYLTLHFMASIERSKNKKHRKPNVLIVCATGIGTSK HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHH FISNKLKSIFDINIIDTISSHTMEKILKYNKNIDLIVTTIPLKVKGIKCIEVNTFLTEKN HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECEEECCEEEEEEEHHHHHCC ISELGLYFAKFIRDNSEECNSSCKYEERDKVQEILNIVKENCTIHDYYKLRNKLALYLDI HHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEEEE KDPALTENHKPSLKELLKPDFIKLNEEAKDWEDAVRKSGEILMNNGCIKESYIDAMVNTV CCCCCCCCCCCHHHHHCCCCCEEECCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH KNMGPYIVIAPGIAMPHAAPEDGVLKTGISMLTLKDPISFGNSEHDPVSVIISICSIDKV HHCCCEEEEECCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCHH NHMKALKELMSIMDQEDFISKVKSMKTPSEIDPILYS HHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC >Mature Secondary Structure MLNKRCSNILQMIVNNERPITIKEISKKVNKSPRTVRYDLDKIDDYLTEIEFPKLERKSN CCCHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCCCCC LGISLDLKDEEIKKLFKIIGKINNYDYVLSQKERVFYIIYELLNKSEFVTINMLSDRMMV CCEEEECCHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCEEEEEEHHHHHHH SRSTIINDLIEVKKWLLENKITLESSKGQGIKILGRERDLRRAAVKLFFQSMDSINFFNV HHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEE TTLKLFNDIDIDFIRNTIKIAEEQMETSFSDDAFNNLVIHIAIAIKRIELSKDIIMDSEE EEEEHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEHHHHHHHHHHHHHCCHHH LKNLRKTAEYAIASGVAKMLEDRFKIFIPEDEIGYITIHILGSNTSTLENIVKDDWIYLH HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHH LIVFKLIENVENITGINFSKDNKLFDSLAQHIRPAIYRLKHDIKVKNPLIEEIKEKYSYI HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH FESIEEGVKFIEEDIGDSVNQEEIGYLTLHFMASIERSKNKKHRKPNVLIVCATGIGTSK HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHH FISNKLKSIFDINIIDTISSHTMEKILKYNKNIDLIVTTIPLKVKGIKCIEVNTFLTEKN HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECEEECCEEEEEEEHHHHHCC ISELGLYFAKFIRDNSEECNSSCKYEERDKVQEILNIVKENCTIHDYYKLRNKLALYLDI HHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEEEEE KDPALTENHKPSLKELLKPDFIKLNEEAKDWEDAVRKSGEILMNNGCIKESYIDAMVNTV CCCCCCCCCCCHHHHHCCCCCEEECCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHH KNMGPYIVIAPGIAMPHAAPEDGVLKTGISMLTLKDPISFGNSEHDPVSVIISICSIDKV HHCCCEEEEECCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCHH NHMKALKELMSIMDQEDFISKVKSMKTPSEIDPILYS HHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9384377 [H]