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The map label for this gene is cynR [H]

Identifier: 170754217

GI number: 170754217

Start: 773361

End: 774236

Strand: Direct

Name: cynR [H]

Synonym: CLD_0109

Alternate gene names: 170754217

Gene position: 773361-774236 (Clockwise)

Preceding gene: 170756216

Following gene: 170755753

Centisome position: 19.54

GC content: 29.91

Gene sequence:

>876_bases
ATGATAAGCAAATTAGATTTATATAGAGTATTTGGCTGTGTAGGAGAGTGCAAAAGCTTTTCAAAGGCAGCTAAGGAGTT
ATACATGACACAACCGGCTGTAAGTCAATCAATAATGCAATTAGAAAAAGAATTAGATATAAGACTTTTTACACGAACTC
CTAAAGGGGTTATTTTAACTAATGAAGGTGAAATTTTATTTGAATATGTAACTTCTGCTATAAGTTTAATAGAAGCAGGA
GAAAAAAAGATGCTAGATTCTAAAAATCTTATGATTGGAGATCTAAAAATAGGGGTAGGGGATACTACTTCTAAGTATTT
TTTACTATCTTATTTAGAAGAATTTCATATAAAGTATCCAAATGTAAAATTAAAAATATTAAACGGAACTACTGTAGAGT
TGTGTAACTTGGTTAAAACAGGGGATATTGATTTGGCTATCTGTAACTTACCAATAGAAGACTCTGCTCTAGAATTTAAA
AAGTGTATGGAAATACAAGATATATTTGTTTGTGGGGAAAGGCTTAATAAAACTATATCTGAGCCTTTAAGTTTTGTAGA
AATCTCAAAGATGCCCCTTATATTATTAGATGGCAAATCTAACTCAAGACAATATGTAGAAAATTATTTATTGTCAAAGG
GGGTCAATATCAGCCCAGAAATAGAGCTTGGTGCTCATGATTTATTATTACAATTTGCAAAAATAAATCTAGGAATAGCC
TGTGTTGTAAAGGAATTTTCATTAGAATATTTAGAACAAGAAATTTTGTTTGAAGTAAAAACTAATGAACCTATACCTAA
GCGCAATATAGGATTTTGTTATTTAAAAAGTGTATCACTGTCCCCAGCTGCAAAAACATTTTTAGAAACCCTATAA

Upstream 100 bases:

>100_bases
AAATTTTGAATTAAAATTACTTCCTATACTTGTAATATACAACATTTTTATGGTATAAGTAAAATAAATAGTATATATAG
TGGGTATAAGGAGGGCTTAT

Downstream 100 bases:

>100_bases
AACTCTGCATTTTATTCAAATGTTATTAAATATAGGGTATACTATAAATAAAATAAACAAAATTAAATACTAAGAAATAG
ATTGGTAAAGAATAGATGAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MISKLDLYRVFGCVGECKSFSKAAKELYMTQPAVSQSIMQLEKELDIRLFTRTPKGVILTNEGEILFEYVTSAISLIEAG
EKKMLDSKNLMIGDLKIGVGDTTSKYFLLSYLEEFHIKYPNVKLKILNGTTVELCNLVKTGDIDLAICNLPIEDSALEFK
KCMEIQDIFVCGERLNKTISEPLSFVEISKMPLILLDGKSNSRQYVENYLLSKGVNISPEIELGAHDLLLQFAKINLGIA
CVVKEFSLEYLEQEILFEVKTNEPIPKRNIGFCYLKSVSLSPAAKTFLETL

Sequences:

>Translated_291_residues
MISKLDLYRVFGCVGECKSFSKAAKELYMTQPAVSQSIMQLEKELDIRLFTRTPKGVILTNEGEILFEYVTSAISLIEAG
EKKMLDSKNLMIGDLKIGVGDTTSKYFLLSYLEEFHIKYPNVKLKILNGTTVELCNLVKTGDIDLAICNLPIEDSALEFK
KCMEIQDIFVCGERLNKTISEPLSFVEISKMPLILLDGKSNSRQYVENYLLSKGVNISPEIELGAHDLLLQFAKINLGIA
CVVKEFSLEYLEQEILFEVKTNEPIPKRNIGFCYLKSVSLSPAAKTFLETL
>Mature_291_residues
MISKLDLYRVFGCVGECKSFSKAAKELYMTQPAVSQSIMQLEKELDIRLFTRTPKGVILTNEGEILFEYVTSAISLIEAG
EKKMLDSKNLMIGDLKIGVGDTTSKYFLLSYLEEFHIKYPNVKLKILNGTTVELCNLVKTGDIDLAICNLPIEDSALEFK
KCMEIQDIFVCGERLNKTISEPLSFVEISKMPLILLDGKSNSRQYVENYLLSKGVNISPEIELGAHDLLLQFAKINLGIA
CVVKEFSLEYLEQEILFEVKTNEPIPKRNIGFCYLKSVSLSPAAKTFLETL

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=211, Percent_Identity=26.5402843601896, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787601, Length=171, Percent_Identity=30.4093567251462, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788748, Length=283, Percent_Identity=22.6148409893993, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693105, Length=291, Percent_Identity=24.3986254295533, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786401, Length=151, Percent_Identity=31.7880794701987, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788481, Length=284, Percent_Identity=22.887323943662, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=294, Percent_Identity=23.469387755102, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787128, Length=134, Percent_Identity=34.3283582089552, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788706, Length=145, Percent_Identity=33.7931034482759, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789639, Length=173, Percent_Identity=25.4335260115607, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=23.2876712328767, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788296, Length=230, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786448, Length=128, Percent_Identity=29.6875, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=23.1707317073171, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=236, Percent_Identity=23.3050847457627, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789173, Length=144, Percent_Identity=31.9444444444444, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787589, Length=117, Percent_Identity=29.9145299145299, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790399, Length=144, Percent_Identity=25.6944444444444, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082132, Length=291, Percent_Identity=23.0240549828179, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32723; Mature: 32723

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKLDLYRVFGCVGECKSFSKAAKELYMTQPAVSQSIMQLEKELDIRLFTRTPKGVILT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCCEEEE
NEGEILFEYVTSAISLIEAGEKKMLDSKNLMIGDLKIGVGDTTSKYFLLSYLEEFHIKYP
CCCCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCEECC
NVKLKILNGTTVELCNLVKTGDIDLAICNLPIEDSALEFKKCMEIQDIFVCGERLNKTIS
CEEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EPLSFVEISKMPLILLDGKSNSRQYVENYLLSKGVNISPEIELGAHDLLLQFAKINLGIA
CCHHHEECCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCE
CVVKEFSLEYLEQEILFEVKTNEPIPKRNIGFCYLKSVSLSPAAKTFLETL
EHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHC
>Mature Secondary Structure
MISKLDLYRVFGCVGECKSFSKAAKELYMTQPAVSQSIMQLEKELDIRLFTRTPKGVILT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCCEEEE
NEGEILFEYVTSAISLIEAGEKKMLDSKNLMIGDLKIGVGDTTSKYFLLSYLEEFHIKYP
CCCCHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCEECC
NVKLKILNGTTVELCNLVKTGDIDLAICNLPIEDSALEFKKCMEIQDIFVCGERLNKTIS
CEEEEEECCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EPLSFVEISKMPLILLDGKSNSRQYVENYLLSKGVNISPEIELGAHDLLLQFAKINLGIA
CCHHHEECCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCE
CVVKEFSLEYLEQEILFEVKTNEPIPKRNIGFCYLKSVSLSPAAKTFLETL
EHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]