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The map label for this gene is 170751692

Identifier: 170751692

GI number: 170751692

Start: 5630675

End: 5632120

Strand: Direct

Name: 170751692

Synonym: Mrad2831_5322

Alternate gene names: NA

Gene position: 5630675-5632120 (Clockwise)

Preceding gene: 170751691

Following gene: 170751693

Centisome position: 92.64

GC content: 73.31

Gene sequence:

>1446_bases
ATGCAGGACGCGCGACAGGACGGCATCACCGCCATGGGCGCCGTGGACCTCGCCGCCGCCATCCGCGGCCGGGTCGTCTC
CTGCGTCGAGGTGATGCGCGCCTATCTGGAGCGGATCCATCGGATCAACCCGCAGGTGAACGCCCTCGTGGGCCTGGCCG
ACGACGCCGCGCTCCTGCGGGAGGCCGAGGCCGCCGACGCCGTCCTGGCGCGCGGTGAGCCCGTCGGCCCGCTGCACGGC
TTCCCGCTGGCGGTGAAGGATCTCGACCCGGTCCGCGGCCTGCGGTTCACGCAGGGCTCGCCGATCTTCGCCGACCGGGT
CGCCGAGACGGACTCGATCATGGTCACCCGCCTGCGCGCCGCCGGGGCGATCTTCATCGGCAAGACCAACATCCCCGAGT
TCGGCCTCGGCTCGCACAGCTTCAACCCGGTCTACGGCGCCACCGGCAACGCCTACGACCCGGCGATGACCGGGGGCGGG
TCGAGCGGCGGCGCCGCCGTGGCGGTGGCGCTGCGGATGCAGCCGGTGGCCGACGGCAGCGACCATGCCGGCTCCCTGCG
GAATCCGCCCGCCTTCAACAACTGCTACGGCCTGCGCCCGAGCTGGGGCCGGATCCCGGCCGAGGCCAAGGACGTGTTCA
GCCCGAATCTCGGCGTGCTCGGGGCGATCGGGCGCACGCCGGGCGATATCGGCCTGATGCTCTCGGTGCTGGCGGGCTAC
GATCCGCGCTGCCCGAACGCCGTCCGGCAGGACCCGTCCGCCTTCGCGCGCCCGCTCGCCCGCGACTTCCGCGGGACGCG
GATCGCGTGGCTCGGCGATTGCGGCGGCGAGATCCCGTTCGATCCCGGCGTGCTCGATGTCGGGCGCGCCGCCCTGGCGA
CCTTCGCGGAGATCGGCTGCACCGTCGAGGAGGCGATGCCGCGCTACGACATGGAGCGGCTCTGGCAGGACTGGCTGGTG
CTGCGGGCCCTCACCGTCGCGGCGAACCTCCGCCCGCTCTACGACGAGCCCCGCCACCGGGCCCTGCTCAAGCCGGAGGC
GGTCTGGGAGGTCGAGCGGGGACTGGCGCTCGGCGCCGACCGGATCATCGCGGCGCTGGAGGGGCGCACCCGCTGGTACG
AGACCCTGCGCCGCTTCATGGAGGCCTACGACTACGTGGTCATGCCCAGCGCCCAGGTCTTCCCGTTCGACAAGAGCCTG
CGCTGGCCCGAGACCGTGGGCGGCCACGCGATGGACACCTACCACCGCTGGATGCAGATCACGATTCCCGCGACGATGTC
GGGCCTGCCGGCGCTGGCCGTGCCGGCCGGGTTCGGGGAGGCCGGGCTGCCCGCGGGCATCCAGATCGTCGGTCCCAACC
ACGGCGAGCTCGCCTGCCTGCAGCTCGGTGCCGCCTACGACGCGGCGAGCAACTGGGTCCGCCGCTACCCGCCGCCGCTG
AACTGA

Upstream 100 bases:

>100_bases
ACCCAGACGGATACCGGCCAGCCATCTAATCGGCCGAAGCCATCTTTGCCCGGATCCAACCTCCGGTTACCGATCGTCCG
TCGATCCGGAGGAGGGTAAG

Downstream 100 bases:

>100_bases
GGGAGCGGCTCGTCGACCAAGCCGGCGCAAACAAGCGCCGGACGCGTTGACCCGCCCCGCGCTCTCCGCTAAGAGGCCCG
ACACTTCGCGTGCCGACCAT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQDARQDGITAMGAVDLAAAIRGRVVSCVEVMRAYLERIHRINPQVNALVGLADDAALLREAEAADAVLARGEPVGPLHG
FPLAVKDLDPVRGLRFTQGSPIFADRVAETDSIMVTRLRAAGAIFIGKTNIPEFGLGSHSFNPVYGATGNAYDPAMTGGG
SSGGAAVAVALRMQPVADGSDHAGSLRNPPAFNNCYGLRPSWGRIPAEAKDVFSPNLGVLGAIGRTPGDIGLMLSVLAGY
DPRCPNAVRQDPSAFARPLARDFRGTRIAWLGDCGGEIPFDPGVLDVGRAALATFAEIGCTVEEAMPRYDMERLWQDWLV
LRALTVAANLRPLYDEPRHRALLKPEAVWEVERGLALGADRIIAALEGRTRWYETLRRFMEAYDYVVMPSAQVFPFDKSL
RWPETVGGHAMDTYHRWMQITIPATMSGLPALAVPAGFGEAGLPAGIQIVGPNHGELACLQLGAAYDAASNWVRRYPPPL
N

Sequences:

>Translated_481_residues
MQDARQDGITAMGAVDLAAAIRGRVVSCVEVMRAYLERIHRINPQVNALVGLADDAALLREAEAADAVLARGEPVGPLHG
FPLAVKDLDPVRGLRFTQGSPIFADRVAETDSIMVTRLRAAGAIFIGKTNIPEFGLGSHSFNPVYGATGNAYDPAMTGGG
SSGGAAVAVALRMQPVADGSDHAGSLRNPPAFNNCYGLRPSWGRIPAEAKDVFSPNLGVLGAIGRTPGDIGLMLSVLAGY
DPRCPNAVRQDPSAFARPLARDFRGTRIAWLGDCGGEIPFDPGVLDVGRAALATFAEIGCTVEEAMPRYDMERLWQDWLV
LRALTVAANLRPLYDEPRHRALLKPEAVWEVERGLALGADRIIAALEGRTRWYETLRRFMEAYDYVVMPSAQVFPFDKSL
RWPETVGGHAMDTYHRWMQITIPATMSGLPALAVPAGFGEAGLPAGIQIVGPNHGELACLQLGAAYDAASNWVRRYPPPL
N
>Mature_481_residues
MQDARQDGITAMGAVDLAAAIRGRVVSCVEVMRAYLERIHRINPQVNALVGLADDAALLREAEAADAVLARGEPVGPLHG
FPLAVKDLDPVRGLRFTQGSPIFADRVAETDSIMVTRLRAAGAIFIGKTNIPEFGLGSHSFNPVYGATGNAYDPAMTGGG
SSGGAAVAVALRMQPVADGSDHAGSLRNPPAFNNCYGLRPSWGRIPAEAKDVFSPNLGVLGAIGRTPGDIGLMLSVLAGY
DPRCPNAVRQDPSAFARPLARDFRGTRIAWLGDCGGEIPFDPGVLDVGRAALATFAEIGCTVEEAMPRYDMERLWQDWLV
LRALTVAANLRPLYDEPRHRALLKPEAVWEVERGLALGADRIIAALEGRTRWYETLRRFMEAYDYVVMPSAQVFPFDKSL
RWPETVGGHAMDTYHRWMQITIPATMSGLPALAVPAGFGEAGLPAGIQIVGPNHGELACLQLGAAYDAASNWVRRYPPPL
N

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=27.8688524590164, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=24.5934959349593, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=453, Percent_Identity=25.60706401766, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=256, Percent_Identity=29.296875, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=206, Percent_Identity=32.5242718446602, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=28.9473684210526, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17538254, Length=200, Percent_Identity=30, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=299, Percent_Identity=28.0936454849498, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=299, Percent_Identity=28.0936454849498, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=304, Percent_Identity=25.9868421052632, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=438, Percent_Identity=27.6255707762557, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=205, Percent_Identity=29.2682926829268, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648435, Length=493, Percent_Identity=26.369168356998, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648437, Length=493, Percent_Identity=26.369168356998, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI45550774, Length=493, Percent_Identity=26.369168356998, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24652985, Length=246, Percent_Identity=35.3658536585366, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922090, Length=246, Percent_Identity=35.3658536585366, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652981, Length=246, Percent_Identity=35.3658536585366, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24652983, Length=246, Percent_Identity=35.3658536585366, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=24.7058823529412, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24644968, Length=241, Percent_Identity=30.2904564315353, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648113, Length=451, Percent_Identity=24.8337028824834, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356731, Length=210, Percent_Identity=33.8095238095238, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648441, Length=427, Percent_Identity=23.4192037470726, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=427, Percent_Identity=23.4192037470726, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 51671; Mature: 51671

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDARQDGITAMGAVDLAAAIRGRVVSCVEVMRAYLERIHRINPQVNALVGLADDAALLR
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCHHHHHH
EAEAADAVLARGEPVGPLHGFPLAVKDLDPVRGLRFTQGSPIFADRVAETDSIMVTRLRA
HHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCEECCCCCCHHHHHCCCCHHHHHHHHH
AGAIFIGKTNIPEFGLGSHSFNPVYGATGNAYDPAMTGGGSSGGAAVAVALRMQPVADGS
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCC
DHAGSLRNPPAFNNCYGLRPSWGRIPAEAKDVFSPNLGVLGAIGRTPGDIGLMLSVLAGY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEECCCCCCCHHHHHHHHHHCC
DPRCPNAVRQDPSAFARPLARDFRGTRIAWLGDCGGEIPFDPGVLDVGRAALATFAEIGC
CCCCCHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
TVEEAMPRYDMERLWQDWLVLRALTVAANLRPLYDEPRHRALLKPEAVWEVERGLALGAD
CHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHCHHHCHH
RIIAALEGRTRWYETLRRFMEAYDYVVMPSAQVFPFDKSLRWPETVGGHAMDTYHRWMQI
HHHHHHCCCHHHHHHHHHHHHHCCEEECCCCEECCCCCCCCCCHHCCCCHHHHHHCEEEE
TIPATMSGLPALAVPAGFGEAGLPAGIQIVGPNHGELACLQLGAAYDAASNWVRRYPPPL
EECHHCCCCCCEEECCCCCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCCC
N
C
>Mature Secondary Structure
MQDARQDGITAMGAVDLAAAIRGRVVSCVEVMRAYLERIHRINPQVNALVGLADDAALLR
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCHHHHHH
EAEAADAVLARGEPVGPLHGFPLAVKDLDPVRGLRFTQGSPIFADRVAETDSIMVTRLRA
HHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCEECCCCCCHHHHHCCCCHHHHHHHHH
AGAIFIGKTNIPEFGLGSHSFNPVYGATGNAYDPAMTGGGSSGGAAVAVALRMQPVADGS
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCC
DHAGSLRNPPAFNNCYGLRPSWGRIPAEAKDVFSPNLGVLGAIGRTPGDIGLMLSVLAGY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEECCCCCCCHHHHHHHHHHCC
DPRCPNAVRQDPSAFARPLARDFRGTRIAWLGDCGGEIPFDPGVLDVGRAALATFAEIGC
CCCCCHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
TVEEAMPRYDMERLWQDWLVLRALTVAANLRPLYDEPRHRALLKPEAVWEVERGLALGAD
CHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHCHHHCHH
RIIAALEGRTRWYETLRRFMEAYDYVVMPSAQVFPFDKSLRWPETVGGHAMDTYHRWMQI
HHHHHHCCCHHHHHHHHHHHHHCCEEECCCCEECCCCCCCCCCHHCCCCHHHHHHCEEEE
TIPATMSGLPALAVPAGFGEAGLPAGIQIVGPNHGELACLQLGAAYDAASNWVRRYPPPL
EECHHCCCCCCEEECCCCCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]