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The map label for this gene is ilvI [H]

Identifier: 170751693

GI number: 170751693

Start: 5632545

End: 5634320

Strand: Direct

Name: ilvI [H]

Synonym: Mrad2831_5323

Alternate gene names: 170751693

Gene position: 5632545-5634320 (Clockwise)

Preceding gene: 170751692

Following gene: 170751694

Centisome position: 92.67

GC content: 67.0

Gene sequence:

>1776_bases
ATGGGCGGCGAGGTCATGACCGGGGCCGAGATGGTCATCCGGGCCTTCCAGGATCAGGGTGTCGACACGCTGTTCGGGTA
CCCGGGCGGCGCCGTACTTCCGATCTACGACGCGCTCTTCCACCAGAACGCCGTCAAGCACATCCTCGTCCGCCACGAGC
AGGGCGCCGTCCACGCCGCGGAGGGCTATGCCCGCTCCTCCGGCAAGGTCGGCTGCGTCCTCGTGACGTCGGGCCCCGGC
GCGACCAACATCGTCACCGGACTCACCGACGCGATGCTGGACTCGATCCCGCTGGTCTGCATCACCGGACAGGTCCCGAC
GCACCTGATCGGCACCGACGCGTTCCAGGAATGCGACACGGTCGGCATCACGCGCCACTGCACGAAGCACAATTACCTCG
TCAAATCGATCGACGACCTGCCGCGCATCCTGCACGAGGCGTTCTACGTCGCGGCGAACGGCCGTCCCGGTCCGGTCGTG
GTCGACCTGCCCAAGGACATCCAGTTCGCCTCCGGCCTCTACGAGCGCCCCGAGATCGCGAGCCACAAGACCTACCGCCC
GGCCGTCAAGGGCGACGCCGACCGGATCCGCGCGGCCGTCGAGCTGATGGCGACCGCGCGCCGGCCGGTCTTCTACACCG
GCGGCGGCGTCATCAACTCGGGTCCTGAGGCCTCGCGCCTGCTGCGCGAGCTCGTCCGCGAGACCGGCTTCCCGATCACC
TCGACCCTGATGGGGCTCGGCGCCTATCCGGGCACGGACGAGAAGTTCCTGGGCATGCTCGGCATGCACGGGACCTACGA
GGCCAACCTCGCGATGCACGAGTGCGACGTGATGATCTGCGTCGGCGCCCGGTTCGACGACCGGATCACCGGCCGGCTCG
ACGCGTTCTCGCCGTTCTCGAAGAAGATCCACATCGACGTCGACGCCTCCTCGATCAACAAGGTCGTCAAGGTCGATGTC
GGGATCGTCGGCGACTGCGCGTCGGTGCTCGCCGACATGCTGGAGGCGTGGCGCGCCTTGCCGTCGCGGCCCGACACGTC
GAACCTCGATCCGTGGTTCCAGAAGATCAACGCCTGGAAGGCGCGGGACTGCCTCGCCTACTGGCCGTCGGGCACGATCA
TCAAGCCGCAATACGCCGTGCAGCGCCTCTACGAGGCCTGCAAGGACCGCGAGACCTACATCACCACCGAGGTCGGCCAG
CACCAGATGTGGGCGGCCCAGTACTTCAAGTACGACGAGCCGAACCGGTGGATGACGTCGGGCGGCCTCGGCACGATGGG
CTACGGCCTGCCGGCGGCGATCGGCACGCAGCTCGCGCACCCGAACGGGCTCGTCATCGACATCGCCGGCGAGGCCTCGA
TCCTGATGAACATGCAGGAGATGTCGACGGCGGTGCAGTACCGCCTGCCGATCAAGGTGTTCATCCTGAACAACGAGTAC
ATGGGCATGGTCCGCCAGTGGCAGGAGCTGCTGCACGGCTCGCGCTACTCGCAGAGCTACTCGGAGAGCCTGCCGGACTT
CGTGAAGCTCGCGGAGGCCTACGGCGCCAAGGGCATGCGCTGCGAGAAGCCGGGCGACCTCGACGGCGCCATCGCGGAGA
TGCTCGCCCACGACGGCCCGGTGATCTTCGACTGCATCGTCGACAAGACCGAGAACTGCTTCCCGATGATCCCGTCGGGC
AAGGCGCACAACGAGATGCTCCTGTCCGATTACCTCGGCGAGACCGGCGTCGAACTCGGCGACGTGATCTCCGAGGAAGG
CAAGATGCTGGTGTGA

Upstream 100 bases:

>100_bases
GGCGGAGACCGCCCCGCGTGACGGGGGTGCCGGGAGAGAGAACGGGGCAGGGCGGCGATCACGGGATCGCGGTGGCTCCG
GAGAGCGCGAGGAGACGAGC

Downstream 100 bases:

>100_bases
GGGAGGGCGGCCCGGCTCCGTGCCGGGCCGCGATCCGCCGCTGCGGCGGCGTTGGGTCGAACGACGAGGACTTGCCCCGC
GATGAACGCGATGAACACCC

Product: acetolactate synthase, large subunit type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MGGEVMTGAEMVIRAFQDQGVDTLFGYPGGAVLPIYDALFHQNAVKHILVRHEQGAVHAAEGYARSSGKVGCVLVTSGPG
ATNIVTGLTDAMLDSIPLVCITGQVPTHLIGTDAFQECDTVGITRHCTKHNYLVKSIDDLPRILHEAFYVAANGRPGPVV
VDLPKDIQFASGLYERPEIASHKTYRPAVKGDADRIRAAVELMATARRPVFYTGGGVINSGPEASRLLRELVRETGFPIT
STLMGLGAYPGTDEKFLGMLGMHGTYEANLAMHECDVMICVGARFDDRITGRLDAFSPFSKKIHIDVDASSINKVVKVDV
GIVGDCASVLADMLEAWRALPSRPDTSNLDPWFQKINAWKARDCLAYWPSGTIIKPQYAVQRLYEACKDRETYITTEVGQ
HQMWAAQYFKYDEPNRWMTSGGLGTMGYGLPAAIGTQLAHPNGLVIDIAGEASILMNMQEMSTAVQYRLPIKVFILNNEY
MGMVRQWQELLHGSRYSQSYSESLPDFVKLAEAYGAKGMRCEKPGDLDGAIAEMLAHDGPVIFDCIVDKTENCFPMIPSG
KAHNEMLLSDYLGETGVELGDVISEEGKMLV

Sequences:

>Translated_591_residues
MGGEVMTGAEMVIRAFQDQGVDTLFGYPGGAVLPIYDALFHQNAVKHILVRHEQGAVHAAEGYARSSGKVGCVLVTSGPG
ATNIVTGLTDAMLDSIPLVCITGQVPTHLIGTDAFQECDTVGITRHCTKHNYLVKSIDDLPRILHEAFYVAANGRPGPVV
VDLPKDIQFASGLYERPEIASHKTYRPAVKGDADRIRAAVELMATARRPVFYTGGGVINSGPEASRLLRELVRETGFPIT
STLMGLGAYPGTDEKFLGMLGMHGTYEANLAMHECDVMICVGARFDDRITGRLDAFSPFSKKIHIDVDASSINKVVKVDV
GIVGDCASVLADMLEAWRALPSRPDTSNLDPWFQKINAWKARDCLAYWPSGTIIKPQYAVQRLYEACKDRETYITTEVGQ
HQMWAAQYFKYDEPNRWMTSGGLGTMGYGLPAAIGTQLAHPNGLVIDIAGEASILMNMQEMSTAVQYRLPIKVFILNNEY
MGMVRQWQELLHGSRYSQSYSESLPDFVKLAEAYGAKGMRCEKPGDLDGAIAEMLAHDGPVIFDCIVDKTENCFPMIPSG
KAHNEMLLSDYLGETGVELGDVISEEGKMLV
>Mature_590_residues
GGEVMTGAEMVIRAFQDQGVDTLFGYPGGAVLPIYDALFHQNAVKHILVRHEQGAVHAAEGYARSSGKVGCVLVTSGPGA
TNIVTGLTDAMLDSIPLVCITGQVPTHLIGTDAFQECDTVGITRHCTKHNYLVKSIDDLPRILHEAFYVAANGRPGPVVV
DLPKDIQFASGLYERPEIASHKTYRPAVKGDADRIRAAVELMATARRPVFYTGGGVINSGPEASRLLRELVRETGFPITS
TLMGLGAYPGTDEKFLGMLGMHGTYEANLAMHECDVMICVGARFDDRITGRLDAFSPFSKKIHIDVDASSINKVVKVDVG
IVGDCASVLADMLEAWRALPSRPDTSNLDPWFQKINAWKARDCLAYWPSGTIIKPQYAVQRLYEACKDRETYITTEVGQH
QMWAAQYFKYDEPNRWMTSGGLGTMGYGLPAAIGTQLAHPNGLVIDIAGEASILMNMQEMSTAVQYRLPIKVFILNNEYM
GMVRQWQELLHGSRYSQSYSESLPDFVKLAEAYGAKGMRCEKPGDLDGAIAEMLAHDGPVIFDCIVDKTENCFPMIPSGK
AHNEMLLSDYLGETGVELGDVISEEGKMLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=549, Percent_Identity=24.5901639344262, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI21361361, Length=601, Percent_Identity=23.4608985024958, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=50.6129597197898, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=41.563055062167, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=31.8505338078292, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1787096, Length=554, Percent_Identity=28.8808664259928, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788716, Length=576, Percent_Identity=23.7847222222222, Blast_Score=132, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=25.9325044404973, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=25.9325044404973, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=593, Percent_Identity=24.6205733558179, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=46.3247863247863, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=24.435318275154, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=530, Percent_Identity=21.5094339622642, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=25.3075571177504, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64588; Mature: 64457

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGEVMTGAEMVIRAFQDQGVDTLFGYPGGAVLPIYDALFHQNAVKHILVRHEQGAVHAA
CCCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
EGYARSSGKVGCVLVTSGPGATNIVTGLTDAMLDSIPLVCITGQVPTHLIGTDAFQECDT
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHHCC
VGITRHCTKHNYLVKSIDDLPRILHEAFYVAANGRPGPVVVDLPKDIQFASGLYERPEIA
CCHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCHHHHCCCCCCCCCC
SHKTYRPAVKGDADRIRAAVELMATARRPVFYTGGGVINSGPEASRLLRELVRETGFPIT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHH
STLMGLGAYPGTDEKFLGMLGMHGTYEANLAMHECDVMICVGARFDDRITGRLDAFSPFS
HHHHHCCCCCCCCHHHHHHHCCCCCEECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCC
KKIHIDVDASSINKVVKVDVGIVGDCASVLADMLEAWRALPSRPDTSNLDPWFQKINAWK
CEEEEEECHHHHCCEEEEECCEECHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ARDCLAYWPSGTIIKPQYAVQRLYEACKDRETYITTEVGQHQMWAAQYFKYDEPNRWMTS
HCCCEEECCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEC
GGLGTMGYGLPAAIGTQLAHPNGLVIDIAGEASILMNMQEMSTAVQYRLPIKVFILNNEY
CCCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHEEECCEEEEEECCHH
MGMVRQWQELLHGSRYSQSYSESLPDFVKLAEAYGAKGMRCEKPGDLDGAIAEMLAHDGP
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCC
VIFDCIVDKTENCFPMIPSGKAHNEMLLSDYLGETGVELGDVISEEGKMLV
EEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
GGEVMTGAEMVIRAFQDQGVDTLFGYPGGAVLPIYDALFHQNAVKHILVRHEQGAVHAA
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
EGYARSSGKVGCVLVTSGPGATNIVTGLTDAMLDSIPLVCITGQVPTHLIGTDAFQECDT
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHHCC
VGITRHCTKHNYLVKSIDDLPRILHEAFYVAANGRPGPVVVDLPKDIQFASGLYERPEIA
CCHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCHHHHCCCCCCCCCC
SHKTYRPAVKGDADRIRAAVELMATARRPVFYTGGGVINSGPEASRLLRELVRETGFPIT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHH
STLMGLGAYPGTDEKFLGMLGMHGTYEANLAMHECDVMICVGARFDDRITGRLDAFSPFS
HHHHHCCCCCCCCHHHHHHHCCCCCEECCEEEEECCEEEEECCCCCCCCCCCCCCCCCCC
KKIHIDVDASSINKVVKVDVGIVGDCASVLADMLEAWRALPSRPDTSNLDPWFQKINAWK
CEEEEEECHHHHCCEEEEECCEECHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ARDCLAYWPSGTIIKPQYAVQRLYEACKDRETYITTEVGQHQMWAAQYFKYDEPNRWMTS
HCCCEEECCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEC
GGLGTMGYGLPAAIGTQLAHPNGLVIDIAGEASILMNMQEMSTAVQYRLPIKVFILNNEY
CCCCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHEEECCEEEEEECCHH
MGMVRQWQELLHGSRYSQSYSESLPDFVKLAEAYGAKGMRCEKPGDLDGAIAEMLAHDGP
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCC
VIFDCIVDKTENCFPMIPSGKAHNEMLLSDYLGETGVELGDVISEEGKMLV
EEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]