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The map label for this gene is ccmE

Identifier: 170751373

GI number: 170751373

Start: 5313144

End: 5313656

Strand: Direct

Name: ccmE

Synonym: Mrad2831_4992

Alternate gene names: 170751373

Gene position: 5313144-5313656 (Clockwise)

Preceding gene: 170751372

Following gene: 170751374

Centisome position: 87.42

GC content: 72.12

Gene sequence:

>513_bases
GTGACGCGTAAGAAACGCCGCCTGATCCTGATCGCCGCCTGCGGCAGCGTCCTGGCGCTCGCCGTGGGGCTGATCCTGTA
TGCCATGAGCGGCTCGATCGTGTTCTTCCGCTCGCCCACCGACATCGCCAAGCAGGCCATCGCCCCGGGCACGCGCCTGC
GGCTCGGCGGTCTGGTGAAGGACGGCTCGCTCAGGCGCGGTCCCGACCAGACGGTCGACTTCGCCGTCACCGACACGAAC
GAGACCGTCGAGGTCCACTACAAGGGGTTGCTGCCGGACCTGTTCCGCGAAGGGCAGGGCGTGGTCGCCGAGGGCGTGCT
GGAGCCGGGCGGGCAGTTCCGCGCCGACACCGTGCTCGCCAAGCACGACGAATCCTACATGCCCCGCGAGGTGGCCGACG
CGCTGAAGGCGCAGGGGCGCTGGCAGGAGGGCGGCCCGAACAGGGGCGGCCCGGCGCCGAAGCCCGCCACCGCGGCGGCG
GACAGCACCCTCGGTCCCCGCAGCGAGCGGTGA

Upstream 100 bases:

>100_bases
TTGACCCGCGGGCGCCTGGAGCCGACACCCTCAAGGCTAGAATGAGAACGGTTCCGGTCGGGGCGGGTCTCCGCGCGCCG
GACCGCGGGGTGGAAGTATC

Downstream 100 bases:

>100_bases
GGGAACCCGCATGATCGTCGAGACCGGCCACTTCGCCCTCGCGCTCGCGCTGGCGATCTCCCTCGTCCAGGTGGTGATGC
CGATCTGGGCCGCCCGCTCG

Product: CcmE/CycJ protein

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MTRKKRRLILIAACGSVLALAVGLILYAMSGSIVFFRSPTDIAKQAIAPGTRLRLGGLVKDGSLRRGPDQTVDFAVTDTN
ETVEVHYKGLLPDLFREGQGVVAEGVLEPGGQFRADTVLAKHDESYMPREVADALKAQGRWQEGGPNRGGPAPKPATAAA
DSTLGPRSER

Sequences:

>Translated_170_residues
MTRKKRRLILIAACGSVLALAVGLILYAMSGSIVFFRSPTDIAKQAIAPGTRLRLGGLVKDGSLRRGPDQTVDFAVTDTN
ETVEVHYKGLLPDLFREGQGVVAEGVLEPGGQFRADTVLAKHDESYMPREVADALKAQGRWQEGGPNRGGPAPKPATAAA
DSTLGPRSER
>Mature_169_residues
TRKKRRLILIAACGSVLALAVGLILYAMSGSIVFFRSPTDIAKQAIAPGTRLRLGGLVKDGSLRRGPDQTVDFAVTDTNE
TVEVHYKGLLPDLFREGQGVVAEGVLEPGGQFRADTVLAKHDESYMPREVADALKAQGRWQEGGPNRGGPAPKPATAAAD
STLGPRSER

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family

Homologues:

Organism=Escherichia coli, GI1788525, Length=145, Percent_Identity=46.2068965517241, Blast_Score=123, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCME_METRJ (B1LU06)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001757633.1
- ProteinModelPortal:   B1LU06
- SMR:   B1LU06
- GeneID:   6141060
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_4992
- HOGENOM:   HBG296631
- OMA:   HVEFAVH
- ProtClustDB:   CLSK2330648
- HAMAP:   MF_01959
- InterPro:   IPR004329
- InterPro:   IPR012340
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF03100 CcmE; SSF82093 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 18109; Mature: 17978

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: BINDING 122-122

Signals:

None

Transmembrane regions:

HASH(0x1da0cc48)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKKRRLILIAACGSVLALAVGLILYAMSGSIVFFRSPTDIAKQAIAPGTRLRLGGLVK
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEECCEEE
DGSLRRGPDQTVDFAVTDTNETVEVHYKGLLPDLFREGQGVVAEGVLEPGGQFRADTVLA
CCCCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHCCCCEEEECCCCCCCCEEHHHEEE
KHDESYMPREVADALKAQGRWQEGGPNRGGPAPKPATAAADSTLGPRSER
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure 
TRKKRRLILIAACGSVLALAVGLILYAMSGSIVFFRSPTDIAKQAIAPGTRLRLGGLVK
CCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEECCEEE
DGSLRRGPDQTVDFAVTDTNETVEVHYKGLLPDLFREGQGVVAEGVLEPGGQFRADTVLA
CCCCCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHCCCCEEEECCCCCCCCEEHHHEEE
KHDESYMPREVADALKAQGRWQEGGPNRGGPAPKPATAAADSTLGPRSER
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA