Click here to switch to the map view.

The map label for this gene is appB [H]

Identifier: 170751147

GI number: 170751147

Start: 5076182

End: 5077165

Strand: Reverse

Name: appB [H]

Synonym: Mrad2831_4763

Alternate gene names: 170751147

Gene position: 5077165-5076182 (Counterclockwise)

Preceding gene: 170751148

Following gene: 170751146

Centisome position: 83.54

GC content: 71.65

Gene sequence:

>984_bases
ATGACGGGCCTGCGCAGGCTCGGCGCGACGGCGGCGAACGCCGCCGGCCTGCTGCTCGCCGTGGTGGTGCTGAACTTCCT
GCTGATCCAGGCGGCGCCGGGCGATCCGGCGCAGGTCATCGCCGGGGAGATGGGCGGCGCCTCGCCGGAGATCATGGCCG
AGATCCGCACCCGCTACGGCCTCGACCGCGGCCTCCCCGAGCAGCTCGCGACCTATGTCGGCAAGGTGGTGCGGGGGGAT
CTCGGCTACTCGTTCTACTTCAACGAGCCGGTCTCGCGCCTGATCCTGGGTCGTCTGCCGGCCACCGCGCTGCTGGTTCT
GAGTTCCCTCGCCCTCGCGGTGCTGGCCGGGGCCGGCCTCGGGATCCTGGGTGCCCGCAGGCCGAACGGCCTCGTCAGTC
ACGCCGTCTCCCTGGTGGCGATCGCCGGCAACGCCGCTCCGGTGTTCTGGACCGGCCTGATGCTGCTGGTCGTGTTCGCC
TCCCTGTGGCCGGTGCTCCCGGTCGCCGGGATGGAGGACGTGGCCACCCCACGCCACGGCCTCGCCTACGCCCTGGACGT
CGCCCGCCACCTGGTCCTGCCGGCGCTGACCCTCGGCATCGTCTACGTCGCGCAGTACAGCCGCCTGATGCGCGCCAGCA
TGATCGACGCGCTCAACGCCGACTACGTCCGCACCGCCCGGGCCAAGGGCCTGCCGGAGCGGACCGTGATCGGCAAGCAC
GCGCTGCGCAACGCGCTGATCCCCCTCGTCACCATGGTCGGGCTCCAGTTCGGGCAGCTCTTCGCGGGGGCGATCCTGGT
CGAGACCGTGTTCGCGTGGCCGGGTCTCGGCCGGCTGACTTTCGACTCGATCCTGCGGCGCGACTACCCGACGCTGCTCG
GCATCCTGTTCTGCTCGGCGCTGCTGGTCATCGCCGCGAACCTCGTCACCGACCTCCTCTACCGCCTGATCGACCCGCGG
ATCCGCGCCGGGAGGCCGGCATGA

Upstream 100 bases:

>100_bases
TCTACGACAAGCGCCTGACGAACCTGCCCCTGTCGATCTGGGGGCCGCTCTCGCCCCTCGACGAGGTCGCCTGGGCGCAG
GGCGCCGCCGGAGCCGAGCG

Downstream 100 bases:

>100_bases
GCGCCCTCGCCGCGGCCGAACCGGCGGTGCGGCCCCAGCCGGTGCGATCCGAGACCGCGCCACCGATGCCGCGCCCGGCG
GCGGATCCCGGCCTCGCCGC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTGLRRLGATAANAAGLLLAVVVLNFLLIQAAPGDPAQVIAGEMGGASPEIMAEIRTRYGLDRGLPEQLATYVGKVVRGD
LGYSFYFNEPVSRLILGRLPATALLVLSSLALAVLAGAGLGILGARRPNGLVSHAVSLVAIAGNAAPVFWTGLMLLVVFA
SLWPVLPVAGMEDVATPRHGLAYALDVARHLVLPALTLGIVYVAQYSRLMRASMIDALNADYVRTARAKGLPERTVIGKH
ALRNALIPLVTMVGLQFGQLFAGAILVETVFAWPGLGRLTFDSILRRDYPTLLGILFCSALLVIAANLVTDLLYRLIDPR
IRAGRPA

Sequences:

>Translated_327_residues
MTGLRRLGATAANAAGLLLAVVVLNFLLIQAAPGDPAQVIAGEMGGASPEIMAEIRTRYGLDRGLPEQLATYVGKVVRGD
LGYSFYFNEPVSRLILGRLPATALLVLSSLALAVLAGAGLGILGARRPNGLVSHAVSLVAIAGNAAPVFWTGLMLLVVFA
SLWPVLPVAGMEDVATPRHGLAYALDVARHLVLPALTLGIVYVAQYSRLMRASMIDALNADYVRTARAKGLPERTVIGKH
ALRNALIPLVTMVGLQFGQLFAGAILVETVFAWPGLGRLTFDSILRRDYPTLLGILFCSALLVIAANLVTDLLYRLIDPR
IRAGRPA
>Mature_326_residues
TGLRRLGATAANAAGLLLAVVVLNFLLIQAAPGDPAQVIAGEMGGASPEIMAEIRTRYGLDRGLPEQLATYVGKVVRGDL
GYSFYFNEPVSRLILGRLPATALLVLSSLALAVLAGAGLGILGARRPNGLVSHAVSLVAIAGNAAPVFWTGLMLLVVFAS
LWPVLPVAGMEDVATPRHGLAYALDVARHLVLPALTLGIVYVAQYSRLMRASMIDALNADYVRTARAKGLPERTVIGKHA
LRNALIPLVTMVGLQFGQLFAGAILVETVFAWPGLGRLTFDSILRRDYPTLLGILFCSALLVIAANLVTDLLYRLIDPRI
RAGRPA

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=339, Percent_Identity=38.6430678466077, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1787053, Length=305, Percent_Identity=41.3114754098361, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1787761, Length=320, Percent_Identity=40, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789888, Length=307, Percent_Identity=34.5276872964169, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787497, Length=306, Percent_Identity=32.6797385620915, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1788504, Length=346, Percent_Identity=30.9248554913295, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787550, Length=269, Percent_Identity=30.1115241635688, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34646; Mature: 34514

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLRRLGATAANAAGLLLAVVVLNFLLIQAAPGDPAQVIAGEMGGASPEIMAEIRTRYG
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHC
LDRGLPEQLATYVGKVVRGDLGYSFYFNEPVSRLILGRLPATALLVLSSLALAVLAGAGL
CCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
GILGARRPNGLVSHAVSLVAIAGNAAPVFWTGLMLLVVFASLWPVLPVAGMEDVATPRHG
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHH
LAYALDVARHLVLPALTLGIVYVAQYSRLMRASMIDALNADYVRTARAKGLPERTVIGKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHH
ALRNALIPLVTMVGLQFGQLFAGAILVETVFAWPGLGRLTFDSILRRDYPTLLGILFCSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLVIAANLVTDLLYRLIDPRIRAGRPA
HHHHHHHHHHHHHHHHHCCHHCCCCCC
>Mature Secondary Structure 
TGLRRLGATAANAAGLLLAVVVLNFLLIQAAPGDPAQVIAGEMGGASPEIMAEIRTRYG
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHC
LDRGLPEQLATYVGKVVRGDLGYSFYFNEPVSRLILGRLPATALLVLSSLALAVLAGAGL
CCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
GILGARRPNGLVSHAVSLVAIAGNAAPVFWTGLMLLVVFASLWPVLPVAGMEDVATPRHG
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHH
LAYALDVARHLVLPALTLGIVYVAQYSRLMRASMIDALNADYVRTARAKGLPERTVIGKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHH
ALRNALIPLVTMVGLQFGQLFAGAILVETVFAWPGLGRLTFDSILRRDYPTLLGILFCSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLVIAANLVTDLLYRLIDPRIRAGRPA
HHHHHHHHHHHHHHHHHCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]