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The map label for this gene is mcl2 [H]

Identifier: 170750523

GI number: 170750523

Start: 4386181

End: 4387038

Strand: Direct

Name: mcl2 [H]

Synonym: Mrad2831_4130

Alternate gene names: 170750523

Gene position: 4386181-4387038 (Clockwise)

Preceding gene: 170750522

Following gene: 170750524

Centisome position: 72.17

GC content: 73.66

Gene sequence:

>858_bases
ATGCGCTCCAAGCTGTTCGTCCCGGCCTCCCGGCCCGAATTGTTCGCGAAGGCCGCCACCGGCCCCGCCGACGCCCTCTC
CTTCGACCTGGAGGACGCCGTGCCGGAAGCCCGCAAGGACGAGGCGCGGGACACGCTCGCGACCTATCTGCGGGAAGCGC
CGACCACCCTGCGCAAGGCGGTCGTGGTGCGGGTCAACGCCCCCGGCACCGGGCATTTCGCGCGCGACCTCGAGGCGGTG
TCCCTGCCGCGCACGGATCTCATCAACCTGCCGATGGTCGAGGATCCGGCCGCCATCATCGAGGCGGCCGCGCGCCTCGA
CCGCCTCGATCCGACGGGTCGCATTCGCCTCCTCGTCAACATCGAGACCCCGAGAAGCCTCCGCCGGGCGGCTGACCTCG
CCTGCGCGCATCCCCGCGTCGCCGGCCTGCAGATCGGCTACGCCGATCTGCTGGAGCCGTGCGGCATCGTCCGGCACGAC
GAAGCCGCCCTCGCTCAGATCCGCGTCGCCGTCCGGCTCGCCGCCGCCGAGGCCGGCCTGCCCGCCTATGACGGAGCCTT
CGCGGCGGTGAGGGACCCCGAGGCCTTCCGCGCCGAGTGCGAGGCGGCCCGCCGCCACGGCTTCGCGGGCAAGACCTGTA
TCCACCCGTCCCAGGTCGACATCGCCAACGCCGCCTTCCTGCCGAGCCCGGACGCGGTGGCGTGGTCGCGCCGCATCCTG
GCGGCGGCCGCCGAGGCCGAGGCGCGGGGTCTCGGCGCCATCCTCGTGGACGGGCGGATGATCGATCCGCCCTTCCTGGC
CCGCGCCCGCGCCGTCGTGGCGCTGTCCGATCTCCACACCGCGACGGAAGTCCCATGA

Upstream 100 bases:

>100_bases
CGCCCAGGTCGCGACGCGGATCGGCGGGGCCCTCACCGTCACCGTGGCCATCGTCGCGATGGCGCATTTCAAGATCGCCT
GACGGAGGCGCGCCGTGCTG

Downstream 100 bases:

>100_bases
CCGAGCGCGAACTGCACCGGCGCGCCTACCGACCCGAGGCCCGGGGTATCCTCGACGGGGTGCGCATCCTCGACCTCTCC
CGGCTCTTCGCCGGCAACGT

Product: citrate (pro-3S)-lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MRSKLFVPASRPELFAKAATGPADALSFDLEDAVPEARKDEARDTLATYLREAPTTLRKAVVVRVNAPGTGHFARDLEAV
SLPRTDLINLPMVEDPAAIIEAAARLDRLDPTGRIRLLVNIETPRSLRRAADLACAHPRVAGLQIGYADLLEPCGIVRHD
EAALAQIRVAVRLAAAEAGLPAYDGAFAAVRDPEAFRAECEAARRHGFAGKTCIHPSQVDIANAAFLPSPDAVAWSRRIL
AAAAEAEARGLGAILVDGRMIDPPFLARARAVVALSDLHTATEVP

Sequences:

>Translated_285_residues
MRSKLFVPASRPELFAKAATGPADALSFDLEDAVPEARKDEARDTLATYLREAPTTLRKAVVVRVNAPGTGHFARDLEAV
SLPRTDLINLPMVEDPAAIIEAAARLDRLDPTGRIRLLVNIETPRSLRRAADLACAHPRVAGLQIGYADLLEPCGIVRHD
EAALAQIRVAVRLAAAEAGLPAYDGAFAAVRDPEAFRAECEAARRHGFAGKTCIHPSQVDIANAAFLPSPDAVAWSRRIL
AAAAEAEARGLGAILVDGRMIDPPFLARARAVVALSDLHTATEVP
>Mature_285_residues
MRSKLFVPASRPELFAKAATGPADALSFDLEDAVPEARKDEARDTLATYLREAPTTLRKAVVVRVNAPGTGHFARDLEAV
SLPRTDLINLPMVEDPAAIIEAAARLDRLDPTGRIRLLVNIETPRSLRRAADLACAHPRVAGLQIGYADLLEPCGIVRHD
EAALAQIRVAVRLAAAEAGLPAYDGAFAAVRDPEAFRAECEAARRHGFAGKTCIHPSQVDIANAAFLPSPDAVAWSRRIL
AAAAEAEARGLGAILVDGRMIDPPFLARARAVVALSDLHTATEVP

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=295, Percent_Identity=27.7966101694915, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI87081764, Length=283, Percent_Identity=31.8021201413428, Blast_Score=124, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17557458, Length=298, Percent_Identity=28.8590604026846, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6; 4.1.3.34

Molecular weight: Translated: 30389; Mature: 30389

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKLFVPASRPELFAKAATGPADALSFDLEDAVPEARKDEARDTLATYLREAPTTLRKA
CCCCEECCCCCCHHHHHCCCCCHHHHEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEE
VVVRVNAPGTGHFARDLEAVSLPRTDLINLPMVEDPAAIIEAAARLDRLDPTGRIRLLVN
EEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
IETPRSLRRAADLACAHPRVAGLQIGYADLLEPCGIVRHDEAALAQIRVAVRLAAAEAGL
ECCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHCCC
PAYDGAFAAVRDPEAFRAECEAARRHGFAGKTCIHPSQVDIANAAFLPSPDAVAWSRRIL
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHH
AAAAEAEARGLGAILVDGRMIDPPFLARARAVVALSDLHTATEVP
HHHHHHHHCCCEEEEECCEECCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRSKLFVPASRPELFAKAATGPADALSFDLEDAVPEARKDEARDTLATYLREAPTTLRKA
CCCCEECCCCCCHHHHHCCCCCHHHHEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEE
VVVRVNAPGTGHFARDLEAVSLPRTDLINLPMVEDPAAIIEAAARLDRLDPTGRIRLLVN
EEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
IETPRSLRRAADLACAHPRVAGLQIGYADLLEPCGIVRHDEAALAQIRVAVRLAAAEAGL
ECCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHCCC
PAYDGAFAAVRDPEAFRAECEAARRHGFAGKTCIHPSQVDIANAAFLPSPDAVAWSRRIL
CCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHH
AAAAEAEARGLGAILVDGRMIDPPFLARARAVVALSDLHTATEVP
HHHHHHHHCCCEEEEECCEECCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA