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The map label for this gene is nadE [H]

Identifier: 170750148

GI number: 170750148

Start: 3992327

End: 3994426

Strand: Direct

Name: nadE [H]

Synonym: Mrad2831_3750

Alternate gene names: 170750148

Gene position: 3992327-3994426 (Clockwise)

Preceding gene: 170750145

Following gene: 170750149

Centisome position: 65.69

GC content: 71.67

Gene sequence:

>2100_bases
ATGACCCGCAGCCACCCCTCATCCGGACAGGTGCCCGTGTCTCCAGCCACCTCCGAATCGCCGGCGCGCTTCCGCTCCCT
CTATCGCCACGGGTTCGCCCGGGTCGCGGCCTGCACCGGCCGCAGCCATCCGGCCGAGCCCGAGCGCAACGCCGACGTCA
TCCTCGACCTCGCCCGGACCTGCCACGATTCGGGGGCGGCGCTGGCGGTCTTCACCGAGCTCGGCCTCTCGGCCTACGCG
ATCGAGGACCTGCTCTTGCAGCAGACGCTGCTGGACGCGGTCGAGGCCGCCGCAGCCCGGGTGATCGCCGAATCGGCCAA
TCTCCGGCCGCTGCTGCTCGTCGGCGCGCCCCTGCGCTGGCGGCACCGCGTCTACAACTGCGCGCTCGCCATCCAGGGCG
GCCGCCTGCTCGGCGCCGTCCCCAAGACATTCCTGCCGAACTACCGGGAATTCTACGAGAAGCGCCACTTCGCCTCGGGC
GCCGGGATCGCCGGCGAGACCGTCCGGGTCGCCGGTCGGGACGCGCCGTTCGGCACCGACCTGCTGTTCCCCGCCGAGGA
CCTGCCGGGCCTCGTGGTCGGGGTGGAGATCTGCGAGGATCTGTGGGTGCCGGAGCCCCCCGGCATGCGCGCGGCGCTCG
CCGGCGCCACCGTGCTGGCGAATCTCTCGGGCAGCCCGATCACCGTCGGCCGGGCCGAGTCGCGGGCGCTCCTCTCCCGG
GCGGCCTCGATGCGGGGCGCCTGCGCGTACGTCTACGCGGCGGCCGGCCAGGGCGAATCGACCACCGACCTGTCCTGGGA
CGGCCAGACCAGCATCGACGAGCTGGGCGTGCGCCTCGCGGAGGGCGAGCGCTTCCCCGAGAAGCCGGTGACGACGCTGG
CCGACATCGACCTCGACCTGATCGCCCAGGAGCGCCTGCAGGCGGGCAGCTTCGACGACGACGCGCGCGGCCACACCCTG
CCCTATCGCCGGGTGCCGTTCCGGGTCGGCCCGCCGGAGGCCGATCTCGGCCTGATCCGGCGGATCGAGCGCTTTCCGTT
CGTGCCGGCCGACCCGTCGCGCCTCGCGCAGGATTGCTACGAGGCCTACAACATCCAGGTCGCCGGCCTCGCCAAGCGCC
TGGAGGCCACCGGCACCCGCAAGGCCATCATCGGCGTCTCGGGCGGCCTCGATTCGACCCACGCGCTGATCGTGATCGCC
AAGGCGTTCGACCGCCTCGGCTACCCGCGCTCGGACATCCTGGCCTACACGCTGCCGGGCTTCGCCACCTCGAACGCGAC
CAAGACCAACGCCCACGCGCTCATGAAGGCGCTGGGCTGCACGGCGGCCGAGATCGACATCCGCCCGGCCGCCAAGCAGA
TGCTCGCCGACATGAACCACCCCTTCGCCAAGGGCGAGGCGGTCTACGACGTCACCTTCGAGAACGTGCAGGCGGGCCTG
CGCACCGACTACCTGTTCCGGCTCGCCAACCACGCGGGCGGCATCGTGGTCGGCACCGGCGACCTGTCGGAGCTGGCACT
CGGCTGGTGCACCTACGGCGTCGGCGACCAGATGAGCCACTACGCGGTCAATGCCGGCGTGCCCAAGACGCTGATCCAGC
ACCTGATCCGCTGGGTGATCGGCAACGGCGAGGTCGGCGCGGACGAGGCCCGCACCCTCCAGGCCGTCCTCGACACCGAG
ATCTCCCCCGAGCTCGTGCCGGTGGACCAGGACGACAGCCCGCAGAGCACGGAGGGCACGATCGGCCCCTACGCGCTGCA
GGATTTCAACCTGTTCTACACGCTGCGCTACGGGTTCCGGCCCTCGAAGATCGCCTTCCTGGCGCTCCACGCCTGGGGCG
ACCGGGAGCGCGGCACCTGGGCGCCGGACTTCCCGGAGACCAAGCGGGTCGCCTACGAACTCCCGGAGATCCGCCGCTGG
CTCGGGGTGTTCCTGACCCGCTACTTCGGCTTCAGCCAGTTCAAGCGCTCGGCGCTCCCGAACGGGCCGAAGGTCTCGGC
CGGCGGCTCCCTGTCGCCCCGGGGCGACTGGCGCGCGCCCTCCGACGGGTCGGCGCGGATCTGGATCGACGAGTTGAACC
GAAACGTCCCTGAAACTTGA

Upstream 100 bases:

>100_bases
TCCCCGTCCCGAGGCGCCGCGGCGCGGTCTCTCGGGATGACGTCGAGAGCGGGCTAGAGTGCGGGCAGGCTCCGCGCCCC
CGACAGTCCGCATCCCCTAT

Downstream 100 bases:

>100_bases
CCGTCACAAACTCGACCGATTGTGCCATTATGAGCACGGTCAAGCCGATTCTTCGCAATGCGAGATCTTGTATTGGGAAA
ATTGGCTTCGAGGGCGGCCA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MTRSHPSSGQVPVSPATSESPARFRSLYRHGFARVAACTGRSHPAEPERNADVILDLARTCHDSGAALAVFTELGLSAYA
IEDLLLQQTLLDAVEAAAARVIAESANLRPLLLVGAPLRWRHRVYNCALAIQGGRLLGAVPKTFLPNYREFYEKRHFASG
AGIAGETVRVAGRDAPFGTDLLFPAEDLPGLVVGVEICEDLWVPEPPGMRAALAGATVLANLSGSPITVGRAESRALLSR
AASMRGACAYVYAAAGQGESTTDLSWDGQTSIDELGVRLAEGERFPEKPVTTLADIDLDLIAQERLQAGSFDDDARGHTL
PYRRVPFRVGPPEADLGLIRRIERFPFVPADPSRLAQDCYEAYNIQVAGLAKRLEATGTRKAIIGVSGGLDSTHALIVIA
KAFDRLGYPRSDILAYTLPGFATSNATKTNAHALMKALGCTAAEIDIRPAAKQMLADMNHPFAKGEAVYDVTFENVQAGL
RTDYLFRLANHAGGIVVGTGDLSELALGWCTYGVGDQMSHYAVNAGVPKTLIQHLIRWVIGNGEVGADEARTLQAVLDTE
ISPELVPVDQDDSPQSTEGTIGPYALQDFNLFYTLRYGFRPSKIAFLALHAWGDRERGTWAPDFPETKRVAYELPEIRRW
LGVFLTRYFGFSQFKRSALPNGPKVSAGGSLSPRGDWRAPSDGSARIWIDELNRNVPET

Sequences:

>Translated_699_residues
MTRSHPSSGQVPVSPATSESPARFRSLYRHGFARVAACTGRSHPAEPERNADVILDLARTCHDSGAALAVFTELGLSAYA
IEDLLLQQTLLDAVEAAAARVIAESANLRPLLLVGAPLRWRHRVYNCALAIQGGRLLGAVPKTFLPNYREFYEKRHFASG
AGIAGETVRVAGRDAPFGTDLLFPAEDLPGLVVGVEICEDLWVPEPPGMRAALAGATVLANLSGSPITVGRAESRALLSR
AASMRGACAYVYAAAGQGESTTDLSWDGQTSIDELGVRLAEGERFPEKPVTTLADIDLDLIAQERLQAGSFDDDARGHTL
PYRRVPFRVGPPEADLGLIRRIERFPFVPADPSRLAQDCYEAYNIQVAGLAKRLEATGTRKAIIGVSGGLDSTHALIVIA
KAFDRLGYPRSDILAYTLPGFATSNATKTNAHALMKALGCTAAEIDIRPAAKQMLADMNHPFAKGEAVYDVTFENVQAGL
RTDYLFRLANHAGGIVVGTGDLSELALGWCTYGVGDQMSHYAVNAGVPKTLIQHLIRWVIGNGEVGADEARTLQAVLDTE
ISPELVPVDQDDSPQSTEGTIGPYALQDFNLFYTLRYGFRPSKIAFLALHAWGDRERGTWAPDFPETKRVAYELPEIRRW
LGVFLTRYFGFSQFKRSALPNGPKVSAGGSLSPRGDWRAPSDGSARIWIDELNRNVPET
>Mature_698_residues
TRSHPSSGQVPVSPATSESPARFRSLYRHGFARVAACTGRSHPAEPERNADVILDLARTCHDSGAALAVFTELGLSAYAI
EDLLLQQTLLDAVEAAAARVIAESANLRPLLLVGAPLRWRHRVYNCALAIQGGRLLGAVPKTFLPNYREFYEKRHFASGA
GIAGETVRVAGRDAPFGTDLLFPAEDLPGLVVGVEICEDLWVPEPPGMRAALAGATVLANLSGSPITVGRAESRALLSRA
ASMRGACAYVYAAAGQGESTTDLSWDGQTSIDELGVRLAEGERFPEKPVTTLADIDLDLIAQERLQAGSFDDDARGHTLP
YRRVPFRVGPPEADLGLIRRIERFPFVPADPSRLAQDCYEAYNIQVAGLAKRLEATGTRKAIIGVSGGLDSTHALIVIAK
AFDRLGYPRSDILAYTLPGFATSNATKTNAHALMKALGCTAAEIDIRPAAKQMLADMNHPFAKGEAVYDVTFENVQAGLR
TDYLFRLANHAGGIVVGTGDLSELALGWCTYGVGDQMSHYAVNAGVPKTLIQHLIRWVIGNGEVGADEARTLQAVLDTEI
SPELVPVDQDDSPQSTEGTIGPYALQDFNLFYTLRYGFRPSKIAFLALHAWGDRERGTWAPDFPETKRVAYELPEIRRWL
GVFLTRYFGFSQFKRSALPNGPKVSAGGSLSPRGDWRAPSDGSARIWIDELNRNVPET

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=692, Percent_Identity=22.6878612716763, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321865, Length=650, Percent_Identity=23.3846153846154, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24641841, Length=283, Percent_Identity=27.9151943462898, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24641843, Length=283, Percent_Identity=27.9151943462898, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 75634; Mature: 75503

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSHPSSGQVPVSPATSESPARFRSLYRHGFARVAACTGRSHPAEPERNADVILDLART
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
CHDSGAALAVFTELGLSAYAIEDLLLQQTLLDAVEAAAARVIAESANLRPLLLVGAPLRW
HCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
RHRVYNCALAIQGGRLLGAVPKTFLPNYREFYEKRHFASGAGIAGETVRVAGRDAPFGTD
HHHHEEEEEEEECCEEEECCCHHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
LLFPAEDLPGLVVGVEICEDLWVPEPPGMRAALAGATVLANLSGSPITVGRAESRALLSR
CCCCCCCCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCEEECCCHHHHHHHH
AASMRGACAYVYAAAGQGESTTDLSWDGQTSIDELGVRLAEGERFPEKPVTTLADIDLDL
HHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCEEECCCCCCCCCCCHHHHCCHHH
IAQERLQAGSFDDDARGHTLPYRRVPFRVGPPEADLGLIRRIERFPFVPADPSRLAQDCY
HHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
EAYNIQVAGLAKRLEATGTRKAIIGVSGGLDSTHALIVIAKAFDRLGYPRSDILAYTLPG
HHHCEEEHHHHHHHHHCCCCEEEEEECCCCCCCHHHEEHHHHHHHHCCCHHHEEEEECCC
FATSNATKTNAHALMKALGCTAAEIDIRPAAKQMLADMNHPFAKGEAVYDVTFENVQAGL
CCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHH
RTDYLFRLANHAGGIVVGTGDLSELALGWCTYGVGDQMSHYAVNAGVPKTLIQHLIRWVI
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCHHEEECCCCHHHHHHHHHHHHH
GNGEVGADEARTLQAVLDTEISPELVPVDQDDSPQSTEGTIGPYALQDFNLFYTLRYGFR
CCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCEEEEEEEECCC
PSKIAFLALHAWGDRERGTWAPDFPETKRVAYELPEIRRWLGVFLTRYFGFSQFKRSALP
CCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
NGPKVSAGGSLSPRGDWRAPSDGSARIWIDELNRNVPET
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCC
>Mature Secondary Structure 
TRSHPSSGQVPVSPATSESPARFRSLYRHGFARVAACTGRSHPAEPERNADVILDLART
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
CHDSGAALAVFTELGLSAYAIEDLLLQQTLLDAVEAAAARVIAESANLRPLLLVGAPLRW
HCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
RHRVYNCALAIQGGRLLGAVPKTFLPNYREFYEKRHFASGAGIAGETVRVAGRDAPFGTD
HHHHEEEEEEEECCEEEECCCHHHCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
LLFPAEDLPGLVVGVEICEDLWVPEPPGMRAALAGATVLANLSGSPITVGRAESRALLSR
CCCCCCCCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCEEECCCHHHHHHHH
AASMRGACAYVYAAAGQGESTTDLSWDGQTSIDELGVRLAEGERFPEKPVTTLADIDLDL
HHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCEEECCCCCCCCCCCHHHHCCHHH
IAQERLQAGSFDDDARGHTLPYRRVPFRVGPPEADLGLIRRIERFPFVPADPSRLAQDCY
HHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
EAYNIQVAGLAKRLEATGTRKAIIGVSGGLDSTHALIVIAKAFDRLGYPRSDILAYTLPG
HHHCEEEHHHHHHHHHCCCCEEEEEECCCCCCCHHHEEHHHHHHHHCCCHHHEEEEECCC
FATSNATKTNAHALMKALGCTAAEIDIRPAAKQMLADMNHPFAKGEAVYDVTFENVQAGL
CCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHH
RTDYLFRLANHAGGIVVGTGDLSELALGWCTYGVGDQMSHYAVNAGVPKTLIQHLIRWVI
HHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCHHEEECCCCHHHHHHHHHHHHH
GNGEVGADEARTLQAVLDTEISPELVPVDQDDSPQSTEGTIGPYALQDFNLFYTLRYGFR
CCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCEEEEEEEECCC
PSKIAFLALHAWGDRERGTWAPDFPETKRVAYELPEIRRWLGVFLTRYFGFSQFKRSALP
CCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
NGPKVSAGGSLSPRGDWRAPSDGSARIWIDELNRNVPET
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]