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The map label for this gene is hprA [H]

Identifier: 170750068

GI number: 170750068

Start: 3900541

End: 3901485

Strand: Reverse

Name: hprA [H]

Synonym: Mrad2831_3669

Alternate gene names: 170750068

Gene position: 3901485-3900541 (Counterclockwise)

Preceding gene: 170750069

Following gene: 170750067

Centisome position: 64.19

GC content: 66.46

Gene sequence:

>945_bases
ATGACCAAGAAGATCGTGTTCCTGGATCGTGAGTCCCTCGATGCCAGGGTCCGACCGTTCAACTTCCCCCACGAATACGT
CGAGCACGAGTCGACCTGGACGCCGGAAGAGATCGTCTCGCGGCTCCAGGGGGCGGAGATCGCGCTGATCAACAAGGTCC
CGATGCGGGCCGAGACGCTGAAGCAGCTCCCCGACCTGAAGCTGATCGCGGTCGCCGCCACCGGCACCGACGTGGTCGAC
AAGGCCCAGGCCAAGGCCCAGGGGGTCACGGTCGTCAACATCCGCAACTACGCGTTCAACACCGTGCCGGAGCACGTGGT
CGGGCTGATGTTCGCCCTGCGGCGGGCGATCGTGCCCTACGCCAACTCGGTGCGGCGCGGCGACTGGGCGAAGTCCAAGC
AGTTCTGCTACTTCGATTACCCGATCTACGACATCGCCGGCTCGACCCTCGGCATCGTCGGCTACGGGGCGCTGGGCAAG
TCGATCGCCAAGCGCGCCGAGGCGCTCGGCATGAAGGTCATCGCCTACGACGTGTTCCCGCAGGACGGGCTCGTGGATTT
CGAGACGATCCTCACCCAGTCCGACGTGATCACGCTGCACGTGCCGCTGACCCCGGACACCAAGGGGATGATCGGCGCCG
CCGAGCTCAAGAAGATGAAGAAGAGCGCCATCCTCATCAACACGGCCCGGGGCGGGCTGGTGGACGAGGCGGCCCTGCTC
GAAGCGCTCAGGGACGGCACCATCGCGGGCGCCGGCTTCGACGTGGTGGCGCAGGAGCCGCCCAAGGACGGCAACATCCT
GTGCGAGGCCGACCTGCCGAACCTGATCGTCACGCCGCACGTCGCCTGGGCGAGCAAGGAGGCGATGCAGATCCTGGCCG
ACCAGCTCGTCGACAACGTCGAGGCGTTCGTGGCGGGCAAGCCGCAGAACGTGGTCGAGAGCTGA

Upstream 100 bases:

>100_bases
GCGCACGCGATCGGGGCGGGACCGGGTTGCCGGGCCCGCCCCGACGCGCAAGTAAGAACAAGACTATCGAGGAAACCGCC
CGTCGTTCGAGAGGGGTCCT

Downstream 100 bases:

>100_bases
GGGCGGCGCGCAGCGCGCCATCATTCCGGGGCGCCGCAGGCGGGCTCGGAACCCAGTGTCGCCGACGGTGTCGGCAGAGA
ACCACCTGAGCGTCTCGATT

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTKKIVFLDRESLDARVRPFNFPHEYVEHESTWTPEEIVSRLQGAEIALINKVPMRAETLKQLPDLKLIAVAATGTDVVD
KAQAKAQGVTVVNIRNYAFNTVPEHVVGLMFALRRAIVPYANSVRRGDWAKSKQFCYFDYPIYDIAGSTLGIVGYGALGK
SIAKRAEALGMKVIAYDVFPQDGLVDFETILTQSDVITLHVPLTPDTKGMIGAAELKKMKKSAILINTARGGLVDEAALL
EALRDGTIAGAGFDVVAQEPPKDGNILCEADLPNLIVTPHVAWASKEAMQILADQLVDNVEAFVAGKPQNVVES

Sequences:

>Translated_314_residues
MTKKIVFLDRESLDARVRPFNFPHEYVEHESTWTPEEIVSRLQGAEIALINKVPMRAETLKQLPDLKLIAVAATGTDVVD
KAQAKAQGVTVVNIRNYAFNTVPEHVVGLMFALRRAIVPYANSVRRGDWAKSKQFCYFDYPIYDIAGSTLGIVGYGALGK
SIAKRAEALGMKVIAYDVFPQDGLVDFETILTQSDVITLHVPLTPDTKGMIGAAELKKMKKSAILINTARGGLVDEAALL
EALRDGTIAGAGFDVVAQEPPKDGNILCEADLPNLIVTPHVAWASKEAMQILADQLVDNVEAFVAGKPQNVVES
>Mature_313_residues
TKKIVFLDRESLDARVRPFNFPHEYVEHESTWTPEEIVSRLQGAEIALINKVPMRAETLKQLPDLKLIAVAATGTDVVDK
AQAKAQGVTVVNIRNYAFNTVPEHVVGLMFALRRAIVPYANSVRRGDWAKSKQFCYFDYPIYDIAGSTLGIVGYGALGKS
IAKRAEALGMKVIAYDVFPQDGLVDFETILTQSDVITLHVPLTPDTKGMIGAAELKKMKKSAILINTARGGLVDEAALLE
ALRDGTIAGAGFDVVAQEPPKDGNILCEADLPNLIVTPHVAWASKEAMQILADQLVDNVEAFVAGKPQNVVES

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=283, Percent_Identity=32.1554770318021, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI145580578, Length=284, Percent_Identity=31.6901408450704, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4557499, Length=284, Percent_Identity=31.6901408450704, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI145580575, Length=284, Percent_Identity=31.6901408450704, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI4557497, Length=271, Percent_Identity=30.9963099630996, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI61743967, Length=271, Percent_Identity=30.9963099630996, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI6912396, Length=254, Percent_Identity=28.740157480315, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789279, Length=291, Percent_Identity=32.9896907216495, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082289, Length=298, Percent_Identity=31.2080536912752, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787645, Length=260, Percent_Identity=28.0769230769231, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI87081824, Length=150, Percent_Identity=32, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788660, Length=290, Percent_Identity=26.8965517241379, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=249, Percent_Identity=34.9397590361446, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25147481, Length=283, Percent_Identity=27.208480565371, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322116, Length=293, Percent_Identity=29.3515358361775, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320925, Length=277, Percent_Identity=30.6859205776173, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324055, Length=251, Percent_Identity=31.0756972111554, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=288, Percent_Identity=26.0416666666667, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324980, Length=200, Percent_Identity=26.5, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921140, Length=249, Percent_Identity=32.9317269076305, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24646446, Length=269, Percent_Identity=33.0855018587361, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646448, Length=269, Percent_Identity=33.0855018587361, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646452, Length=269, Percent_Identity=33.0855018587361, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646450, Length=269, Percent_Identity=33.0855018587361, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI62472511, Length=269, Percent_Identity=33.0855018587361, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28574286, Length=286, Percent_Identity=30.7692307692308, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45552429, Length=287, Percent_Identity=29.9651567944251, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI28574284, Length=287, Percent_Identity=30.3135888501742, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24585514, Length=287, Percent_Identity=30.3135888501742, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28574282, Length=287, Percent_Identity=30.3135888501742, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI45551003, Length=287, Percent_Identity=30.3135888501742, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24585516, Length=315, Percent_Identity=26.3492063492064, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI28571528, Length=253, Percent_Identity=32.0158102766798, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34172; Mature: 34041

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIVFLDRESLDARVRPFNFPHEYVEHESTWTPEEIVSRLQGAEIALINKVPMRAETL
CCCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHH
KQLPDLKLIAVAATGTDVVDKAQAKAQGVTVVNIRNYAFNTVPEHVVGLMFALRRAIVPY
HHCCCCEEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
ANSVRRGDWAKSKQFCYFDYPIYDIAGSTLGIVGYGALGKSIAKRAEALGMKVIAYDVFP
HHHHCCCCCCCCCCEEEECCCHHHHCCCEEEEEEEHHHHHHHHHHHHHHCEEEEEEEECC
QDGLVDFETILTQSDVITLHVPLTPDTKGMIGAAELKKMKKSAILINTARGGLVDEAALL
CCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCHHHHHH
EALRDGTIAGAGFDVVAQEPPKDGNILCEADLPNLIVTPHVAWASKEAMQILADQLVDNV
HHHHCCCEECCCCEEEECCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
EAFVAGKPQNVVES
HHHHCCCCHHHHCC
>Mature Secondary Structure 
TKKIVFLDRESLDARVRPFNFPHEYVEHESTWTPEEIVSRLQGAEIALINKVPMRAETL
CCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHH
KQLPDLKLIAVAATGTDVVDKAQAKAQGVTVVNIRNYAFNTVPEHVVGLMFALRRAIVPY
HHCCCCEEEEEEECCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
ANSVRRGDWAKSKQFCYFDYPIYDIAGSTLGIVGYGALGKSIAKRAEALGMKVIAYDVFP
HHHHCCCCCCCCCCEEEECCCHHHHCCCEEEEEEEHHHHHHHHHHHHHHCEEEEEEEECC
QDGLVDFETILTQSDVITLHVPLTPDTKGMIGAAELKKMKKSAILINTARGGLVDEAALL
CCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCHHHHHH
EALRDGTIAGAGFDVVAQEPPKDGNILCEADLPNLIVTPHVAWASKEAMQILADQLVDNV
HHHHCCCEECCCCEEEECCCCCCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
EAFVAGKPQNVVES
HHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]