Click here to switch to the map view.

The map label for this gene is mtdA [H]

Identifier: 170750067

GI number: 170750067

Start: 3899498

End: 3900361

Strand: Reverse

Name: mtdA [H]

Synonym: Mrad2831_3668

Alternate gene names: 170750067

Gene position: 3900361-3899498 (Counterclockwise)

Preceding gene: 170750068

Following gene: 170750066

Centisome position: 64.17

GC content: 68.87

Gene sequence:

>864_bases
ATGACCAAGAAGCTGCTGTTCCTGTTCGACACCGATGCCGTGCCGAACGTGTTCGACACGGTCGTCGGCTACGACGGCGG
CGCGGACCACATCACCGGCTACGGCGGCGTGAATCCCGACAATGTCGGCGCGCTCGTGGACGGCACGATCTACACCCGCG
GCGGTTCCGAGAAGAAGTCGACGGCGATCTTCGTCGGCGGCGGCAGCATGGCGGCCGGCGAGGAGCTGTTCACCGCCGTC
AAAAAGCGCTTCTTCGGCCCGTTCCGGGTCTCGGTGATGCTCGATTCCAACGGCTCCAACACCACGGCCGCCGCGGGCGT
CGCGCTGGTGGCCAAGGCCGCCGGCTCGCTCCAGGGCAAGAAGGCCGTCGTGCTGGCGGGCACCGGCCCGGTCGGCATGC
GCTCGGCCGCTCTGCTCGCCGGCGAGGGCGCCGAGGTCACCATCGCGGGCCGCGCCCTCGACAAGACCCAGGCCGCCGCG
GACCAGATCAACAAGCGCTTCAAGGTCAACGTGAAGGCCGCCGCGACCCCCGACGAGGCCTCCCGCGTCGCCCTGGTGAA
GGGCACCAACCTCGTGTTCACGGCCGGCGCGATCGGCGTCGAGCTGCTGCCCGAATCCGCCTGGGCGCAGGAATCGTCCA
TCGAGTTCGTCGCTGACTACAACGCCCAGCCTCCGACGGGCATCGGCGGCATCGACGTGATGGACAAGGGCAAGGACCGC
AACGGCAAGCAGGCCTTCGGCGCGCTCGGCATCGGCGGGCTGAAGCTCAAGCTGCACCGGGCCTGCGTCGGCCAGCTCTT
CGAGAGCACCGACAAGGTGCTCGACGCCGAGGAGATCTACGCCCTCGCCAAGACGATGGCTTGA

Upstream 100 bases:

>100_bases
AACCACCTGAGCGTCTCGATTCCGGGTCCCGCCTCGCGGACCCGGAATGACGGGGTGGAGTGTTCACGTCGATGATGCCC
ACCCTGGGCCGAGGTAGAGA

Downstream 100 bases:

>100_bases
GGAGGCGCCGGTGACCGAGACGCATCGCCCCGAGGGCGACAGCATCGAGAAGTTCCTGACGGAGCTGGCGAGCGCCGCCC
CGACCCCCGGGGGCGGCGGC

Product: methylenetetrahydrofolate dehydrogenase (NADP(+))

Products: NA

Alternate protein names: NADP-dependent methylenetetrahydromethanopterin dehydrogenase; Methylenetetrahydrofolate dehydrogenase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTKKLLFLFDTDAVPNVFDTVVGYDGGADHITGYGGVNPDNVGALVDGTIYTRGGSEKKSTAIFVGGGSMAAGEELFTAV
KKRFFGPFRVSVMLDSNGSNTTAAAGVALVAKAAGSLQGKKAVVLAGTGPVGMRSAALLAGEGAEVTIAGRALDKTQAAA
DQINKRFKVNVKAAATPDEASRVALVKGTNLVFTAGAIGVELLPESAWAQESSIEFVADYNAQPPTGIGGIDVMDKGKDR
NGKQAFGALGIGGLKLKLHRACVGQLFESTDKVLDAEEIYALAKTMA

Sequences:

>Translated_287_residues
MTKKLLFLFDTDAVPNVFDTVVGYDGGADHITGYGGVNPDNVGALVDGTIYTRGGSEKKSTAIFVGGGSMAAGEELFTAV
KKRFFGPFRVSVMLDSNGSNTTAAAGVALVAKAAGSLQGKKAVVLAGTGPVGMRSAALLAGEGAEVTIAGRALDKTQAAA
DQINKRFKVNVKAAATPDEASRVALVKGTNLVFTAGAIGVELLPESAWAQESSIEFVADYNAQPPTGIGGIDVMDKGKDR
NGKQAFGALGIGGLKLKLHRACVGQLFESTDKVLDAEEIYALAKTMA
>Mature_286_residues
TKKLLFLFDTDAVPNVFDTVVGYDGGADHITGYGGVNPDNVGALVDGTIYTRGGSEKKSTAIFVGGGSMAAGEELFTAVK
KRFFGPFRVSVMLDSNGSNTTAAAGVALVAKAAGSLQGKKAVVLAGTGPVGMRSAALLAGEGAEVTIAGRALDKTQAAAD
QINKRFKVNVKAAATPDEASRVALVKGTNLVFTAGAIGVELLPESAWAQESSIEFVADYNAQPPTGIGGIDVMDKGKDRN
GKQAFGALGIGGLKLKLHRACVGQLFESTDKVLDAEEIYALAKTMA

Specific function: Catalyzes the dehydrogenation of methylene-H(4)MPT. Can also catalyze the reversible dehydrogenation of methylene-H(4)F with 20-fold lower catalytic efficiency [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015259
- InterPro:   IPR016040 [H]

Pfam domain/function: PF09176 Mpt_N [H]

EC number: =1.5.1.5 [H]

Molecular weight: Translated: 29447; Mature: 29316

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLLFLFDTDAVPNVFDTVVGYDGGADHITGYGGVNPDNVGALVDGTIYTRGGSEKKS
CCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECEEEECCCCCCCC
TAIFVGGGSMAAGEELFTAVKKRFFGPFRVSVMLDSNGSNTTAAAGVALVAKAAGSLQGK
EEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
KAVVLAGTGPVGMRSAALLAGEGAEVTIAGRALDKTQAAADQINKRFKVNVKAAATPDEA
EEEEEECCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCEEEEEEEEECCCCCC
SRVALVKGTNLVFTAGAIGVELLPESAWAQESSIEFVADYNAQPPTGIGGIDVMDKGKDR
CEEEEEECCCEEEEECCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCC
NGKQAFGALGIGGLKLKLHRACVGQLFESTDKVLDAEEIYALAKTMA
CCCHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
TKKLLFLFDTDAVPNVFDTVVGYDGGADHITGYGGVNPDNVGALVDGTIYTRGGSEKKS
CCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECEEEECCCCCCCC
TAIFVGGGSMAAGEELFTAVKKRFFGPFRVSVMLDSNGSNTTAAAGVALVAKAAGSLQGK
EEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
KAVVLAGTGPVGMRSAALLAGEGAEVTIAGRALDKTQAAADQINKRFKVNVKAAATPDEA
EEEEEECCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHCCCEEEEEEEEECCCCCC
SRVALVKGTNLVFTAGAIGVELLPESAWAQESSIEFVADYNAQPPTGIGGIDVMDKGKDR
CEEEEEECCCEEEEECCCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCC
NGKQAFGALGIGGLKLKLHRACVGQLFESTDKVLDAEEIYALAKTMA
CCCHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 9765566; 11297742 [H]