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The map label for this gene is dctA2 [H]

Identifier: 170749419

GI number: 170749419

Start: 3166971

End: 3168281

Strand: Reverse

Name: dctA2 [H]

Synonym: Mrad2831_3012

Alternate gene names: 170749419

Gene position: 3168281-3166971 (Counterclockwise)

Preceding gene: 170749422

Following gene: 170749418

Centisome position: 52.13

GC content: 67.05

Gene sequence:

>1311_bases
TTGAAGCGCTTGTTCGGGTCGCTGTTCGGGCAGGTTGTCGCCGCCCTGATGATCGGCATCCTCGTCGGCCGGCTCTGGCC
CGACTTTGCCGTCACCCTGAAGCCGCTGGGCGACGTCTTCATCAAGCTCATCAAGATGGTTGTCGCGCCGCTGGTCTTCG
GCGTCGTCGTGCACGGCATCGTCGGTGCGGGCGACCTTCGGAAAGTCGGCCGCGTCGGCCTCAAATCCCTGATCTATTTC
GAGGTGGTGACGACCATCGCGCTCACGCTGGGCCTCGTCCTCGCCTACGTCGTCGCGCCCGGTCACGGGATGAACGTCGA
TCTGGCGACGCTGGATGCCAAGTCGCTCGCCAGCTACACCGACCGGGTCGGTCAGGTGACGGGCACCGTCGACTTCCTGA
TGAAGCTGGTGCCGACGACCTTCGTCGACGCCTTCGCCAAGGGCGACATCCTCCAGGTCCTCCTCCTCGCGCTCCTGTTC
GGCTCCGGCCTCTCGCTCCTCGGCGAGCGCGGCAAGCCCCTGGCCGACAGCTTGGAGCGCATCACCGAGGTCCTGTTCCG
GATCATCGGCTTCATCGTGCGGCTGGCGCCGCTCGGCGTGCTCGGCGCGGTCGCCTTCACGGTCGGCAAATACGGCGTCG
GCTCCCTCAAGCAGCTCAGCATGCTGGTGGTGCTGTTCTACGCGGGGGTGGCGCTCTTCGTGGTCCTGGTGCTCGGCGGC
ATCATGCGGCTCGCCGGCTTCAGCCTGTTCAAGCTGCTCGTCTACCTGCGCGAGGAACTGACCGTCGTGGCCGGAACCGC
CTCCTCCGACTGCGTGCTCCCGCAGGTCATGCGCAAGATGGAGCGGCTCGGCATCAAGGAATCGACCGTCGGCCTCGTGA
TTCCGACCGGCTACTCTTTCAACCTCGACGCGTTCTCCCTCTACCTGACCCTCGCCACGGTGTTCATCGCCCAGGCGACC
AACACCGACCTGTCGTTCGGCCACCTGATGCTGATCCTCGGCATCGCGCTCCTGACCTCGAAGGGCGCCCACGGCGTGCC
AGGCTCGGCGATCGTCGTCCTCGCCGCGACGCTCTCGGCGGTTCCCGAGATCCCGGTGATCGGGCTGGTCCTCGTCCTGT
CGGTCGACTGGTTCGTCGGCATCGCCCGCGCGCTCGGGAACCTGATCGGCAATTGCGTGGCGACCGTCGTCATCGCCGCC
TGGGAAGGCGACATCGACCGGGCGCAGGCCAACCGCGTCCTGAACGGCGAGGACACCACCGACACGGCGGAGATCCTCGA
CGCACCGGTCGCGGAGCCGCACACCGCGTGA

Upstream 100 bases:

>100_bases
CCTCAGGCCGCCGCGCGCCGCGATCGCGTCGATCGAACCCGCCCACGAGAGGCGGGGCGTTCGCGGCCGGGTCCGGGCTA
GCCGTTCCAGGGAGGTTGCG

Downstream 100 bases:

>100_bases
CCGATCCCCTGCCCGACGTTTACAGCTTGAAGTCGAATCGCGGAGACCGACCATGAGCGCTGGAGCCTACAATCCCCCCT
GCGGAGGCCTGCCCGGCCAG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKRLFGSLFGQVVAALMIGILVGRLWPDFAVTLKPLGDVFIKLIKMVVAPLVFGVVVHGIVGAGDLRKVGRVGLKSLIYF
EVVTTIALTLGLVLAYVVAPGHGMNVDLATLDAKSLASYTDRVGQVTGTVDFLMKLVPTTFVDAFAKGDILQVLLLALLF
GSGLSLLGERGKPLADSLERITEVLFRIIGFIVRLAPLGVLGAVAFTVGKYGVGSLKQLSMLVVLFYAGVALFVVLVLGG
IMRLAGFSLFKLLVYLREELTVVAGTASSDCVLPQVMRKMERLGIKESTVGLVIPTGYSFNLDAFSLYLTLATVFIAQAT
NTDLSFGHLMLILGIALLTSKGAHGVPGSAIVVLAATLSAVPEIPVIGLVLVLSVDWFVGIARALGNLIGNCVATVVIAA
WEGDIDRAQANRVLNGEDTTDTAEILDAPVAEPHTA

Sequences:

>Translated_436_residues
MKRLFGSLFGQVVAALMIGILVGRLWPDFAVTLKPLGDVFIKLIKMVVAPLVFGVVVHGIVGAGDLRKVGRVGLKSLIYF
EVVTTIALTLGLVLAYVVAPGHGMNVDLATLDAKSLASYTDRVGQVTGTVDFLMKLVPTTFVDAFAKGDILQVLLLALLF
GSGLSLLGERGKPLADSLERITEVLFRIIGFIVRLAPLGVLGAVAFTVGKYGVGSLKQLSMLVVLFYAGVALFVVLVLGG
IMRLAGFSLFKLLVYLREELTVVAGTASSDCVLPQVMRKMERLGIKESTVGLVIPTGYSFNLDAFSLYLTLATVFIAQAT
NTDLSFGHLMLILGIALLTSKGAHGVPGSAIVVLAATLSAVPEIPVIGLVLVLSVDWFVGIARALGNLIGNCVATVVIAA
WEGDIDRAQANRVLNGEDTTDTAEILDAPVAEPHTA
>Mature_436_residues
MKRLFGSLFGQVVAALMIGILVGRLWPDFAVTLKPLGDVFIKLIKMVVAPLVFGVVVHGIVGAGDLRKVGRVGLKSLIYF
EVVTTIALTLGLVLAYVVAPGHGMNVDLATLDAKSLASYTDRVGQVTGTVDFLMKLVPTTFVDAFAKGDILQVLLLALLF
GSGLSLLGERGKPLADSLERITEVLFRIIGFIVRLAPLGVLGAVAFTVGKYGVGSLKQLSMLVVLFYAGVALFVVLVLGG
IMRLAGFSLFKLLVYLREELTVVAGTASSDCVLPQVMRKMERLGIKESTVGLVIPTGYSFNLDAFSLYLTLATVFIAQAT
NTDLSFGHLMLILGIALLTSKGAHGVPGSAIVVLAATLSAVPEIPVIGLVLVLSVDWFVGIARALGNLIGNCVATVVIAA
WEGDIDRAQANRVLNGEDTTDTAEILDAPVAEPHTA

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI40254478, Length=484, Percent_Identity=24.3801652892562, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI4827012, Length=294, Percent_Identity=27.891156462585, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI66773030, Length=439, Percent_Identity=24.8291571753986, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI169790839, Length=440, Percent_Identity=24.7727272727273, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI21314632, Length=399, Percent_Identity=25.8145363408521, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI223468566, Length=267, Percent_Identity=29.2134831460674, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI223468564, Length=267, Percent_Identity=29.2134831460674, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI5032093, Length=266, Percent_Identity=29.3233082706767, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI194239697, Length=263, Percent_Identity=27.3764258555133, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI262359914, Length=381, Percent_Identity=23.6220472440945, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI301601644, Length=167, Percent_Identity=31.1377245508982, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=53.6231884057971, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1790514, Length=435, Percent_Identity=40.9195402298851, Blast_Score=293, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17537407, Length=414, Percent_Identity=25.3623188405797, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71996953, Length=437, Percent_Identity=24.0274599542334, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193206654, Length=443, Percent_Identity=23.2505643340858, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71983099, Length=453, Percent_Identity=22.7373068432671, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=453, Percent_Identity=22.7373068432671, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI193206505, Length=421, Percent_Identity=24.2280285035629, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17541374, Length=405, Percent_Identity=24.6913580246914, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583025, Length=427, Percent_Identity=23.4192037470726, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17137668, Length=427, Percent_Identity=23.4192037470726, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24583023, Length=427, Percent_Identity=23.4192037470726, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI281360483, Length=421, Percent_Identity=25.41567695962, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137666, Length=421, Percent_Identity=25.41567695962, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281360481, Length=421, Percent_Identity=25.41567695962, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45943; Mature: 45943

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]