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The map label for this gene is cysJ [H]

Identifier: 170748202

GI number: 170748202

Start: 1902128

End: 1903897

Strand: Reverse

Name: cysJ [H]

Synonym: Mrad2831_1784

Alternate gene names: 170748202

Gene position: 1903897-1902128 (Counterclockwise)

Preceding gene: 170748203

Following gene: 170748201

Centisome position: 31.33

GC content: 72.99

Gene sequence:

>1770_bases
ATGTCCCGCCCCAATCTCCTCCCGCGCACCGCCCCGTTCGGGGACGGCGAGCGCGCCAGCCTCGACGCGGTGCTCGGCAC
CGCGAGCCCCACGCAGCGCGCCTGGCTCGCCGGCTTCCTGGCCGGGCTCGACGCCGCGGGCGGCCAGCCGGCCGCGGTGC
CGGCCGCCCCGCCCAAGGCCGCCGCGCCGCTCACCGTGATCTACGCCAGCGAATCGGGGAACTCCGAGGCGCTCGCCGGC
AACGTCGCCAAGCTCGCGCGCAAGCAGGGCTTCAAGCCGAAGGTCGTGGACTTCTCCGACCTCGACGTCGCGACCCTGCC
CAAGGCCGGCAAGCTGATCGCCATCGCGGCGACCTGGGGCGAGGGCGAGCCGCCGGCCCGGGCCGTGCGGGCCTACGGCG
AGTTGATGGGGGAGGGCGCCCCGCGCCTCGACGGCGTCGAGTTCGCGGTCCTGTCGCTCGGCGACACCTCCTACGCGGAG
TTCTGCGCCATCGGTAAGGCGCTCGACGCGCGCTTCGAGGCCCTGGGGGCTAAGCGCGCCGCCGAGCGCGCCGACCTCGA
CCTCGACTTCGAGAAGCCCGCCGCCGACTGGATCAAGGGCGCGCTGAAGGCGCTCGCCCCGGCGGAGCAGCCCGACAACG
TCGTGGCGGTGGACTTCGCCCGCGCCGGCGCCGGCGAGGACGACGAGGCCGAGCCGAGCCGCGAGCCCCTGGTGGTCGAG
GTCGTCGAGCACGTGAACCTCAACTCGTCGCGCTCCGACAAGGAGACGATCCACCTCGCGCTGGCCTTCGAGGACGGCGC
GCCGGCCTACGAGCCGGGCGACTCGCTGGAGATCTTCCCCGAGAACGACCCGCAGCTGGTGGACGAGATCCTGAACGCCG
CCGGTCTCGCGGGCGACGAGGCCCTGCGCCGGGCCCTGCTCGCCGAGCGGGACATCACCACGCTCTCGGCCGCCACGATC
GAGCGGTTCGTGAAGGCCACCGGCCACGCGGACGCGCAGACGCTCATCGACAGCGGCGAGGCCAAGGCCTGGGTCGAGGG
CCGGCACCTGATCGACTTGCTGGAGACCTACCCGGCGAAGCTCACGGCCGAGCATATCGGCACGATCACCCGGCCGCTGC
CGCCGCGGGCCTACTCGATCGCCTCCTCCCGCAAGGAGGTGGGCGACGAGGTCCACCTCGCCATCGCGGCGGTGCGCTAC
GAGACCCACGGCCGGGCCCGCTCGGGCGTCGCCTCGGTCCACGTCGCCGACCGGATCCGCGACGGCGCCAAGCTGCGGGT
GCGGCTGAAGCCGAACCGCCACTTCCGCCTGCCGCAGGATCCGGCCACCGACATCATCATGGTCGGCCCCGGCACCGGCG
TCGCGCCGTTCCGCGCCTTCGTGCAGGAGCGCCGGGCCGTGGAAGCCCCCGGGCGGTCGTGGCTGTTCTTCGGCGACCGG
CACTTCACCCACGACTTCCTGTACCAGCTCGAGTGGCAGGACGCCCTGGAGGACGGGTCGCTCACCAAGATCGACGTCGC
CTTCTCGCGGGACCAGCCGGAGAAGGTCTACGTGCAGGACCGGATCACCCAGCACGCGCAGGAGCTGGTCTCCTGGCTGG
ACGGGGGCGCGCATCTCTACGTCTGCGGCGACGCGAAGAACATGGCCAAGGACGTGCGCGCCGCGGTCGTGAAGGCCTAC
GAGACGGCCAAGAGCCTGAGCGCCGCCGACGCCGAGGCGCAGGTCGCGGCTCTGGAGCGCAGCCACCGCTACCAGCAGGA
CGTTTACTGA

Upstream 100 bases:

>100_bases
TTCGCAGCGCAGCGAATGTGATGTCCTATACGCCGCCCGTTCTGCAAAACGAACGTTTCTCGATTGACACAGGCCCTACC
GGCCCCGACATGGTCCCGTG

Downstream 100 bases:

>100_bases
GGACACACCGCTCCCCGCACCGCGGGGGGCCTTCGACGAGATGCGGCAGAGACAGGCGTGACGCAGGTCCCCTCTCCCGG
GCGGGAGAGGGGCAGGGTGA

Product: flavodoxin/nitric oxide synthase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MSRPNLLPRTAPFGDGERASLDAVLGTASPTQRAWLAGFLAGLDAAGGQPAAVPAAPPKAAAPLTVIYASESGNSEALAG
NVAKLARKQGFKPKVVDFSDLDVATLPKAGKLIAIAATWGEGEPPARAVRAYGELMGEGAPRLDGVEFAVLSLGDTSYAE
FCAIGKALDARFEALGAKRAAERADLDLDFEKPAADWIKGALKALAPAEQPDNVVAVDFARAGAGEDDEAEPSREPLVVE
VVEHVNLNSSRSDKETIHLALAFEDGAPAYEPGDSLEIFPENDPQLVDEILNAAGLAGDEALRRALLAERDITTLSAATI
ERFVKATGHADAQTLIDSGEAKAWVEGRHLIDLLETYPAKLTAEHIGTITRPLPPRAYSIASSRKEVGDEVHLAIAAVRY
ETHGRARSGVASVHVADRIRDGAKLRVRLKPNRHFRLPQDPATDIIMVGPGTGVAPFRAFVQERRAVEAPGRSWLFFGDR
HFTHDFLYQLEWQDALEDGSLTKIDVAFSRDQPEKVYVQDRITQHAQELVSWLDGGAHLYVCGDAKNMAKDVRAAVVKAY
ETAKSLSAADAEAQVAALERSHRYQQDVY

Sequences:

>Translated_589_residues
MSRPNLLPRTAPFGDGERASLDAVLGTASPTQRAWLAGFLAGLDAAGGQPAAVPAAPPKAAAPLTVIYASESGNSEALAG
NVAKLARKQGFKPKVVDFSDLDVATLPKAGKLIAIAATWGEGEPPARAVRAYGELMGEGAPRLDGVEFAVLSLGDTSYAE
FCAIGKALDARFEALGAKRAAERADLDLDFEKPAADWIKGALKALAPAEQPDNVVAVDFARAGAGEDDEAEPSREPLVVE
VVEHVNLNSSRSDKETIHLALAFEDGAPAYEPGDSLEIFPENDPQLVDEILNAAGLAGDEALRRALLAERDITTLSAATI
ERFVKATGHADAQTLIDSGEAKAWVEGRHLIDLLETYPAKLTAEHIGTITRPLPPRAYSIASSRKEVGDEVHLAIAAVRY
ETHGRARSGVASVHVADRIRDGAKLRVRLKPNRHFRLPQDPATDIIMVGPGTGVAPFRAFVQERRAVEAPGRSWLFFGDR
HFTHDFLYQLEWQDALEDGSLTKIDVAFSRDQPEKVYVQDRITQHAQELVSWLDGGAHLYVCGDAKNMAKDVRAAVVKAY
ETAKSLSAADAEAQVAALERSHRYQQDVY
>Mature_588_residues
SRPNLLPRTAPFGDGERASLDAVLGTASPTQRAWLAGFLAGLDAAGGQPAAVPAAPPKAAAPLTVIYASESGNSEALAGN
VAKLARKQGFKPKVVDFSDLDVATLPKAGKLIAIAATWGEGEPPARAVRAYGELMGEGAPRLDGVEFAVLSLGDTSYAEF
CAIGKALDARFEALGAKRAAERADLDLDFEKPAADWIKGALKALAPAEQPDNVVAVDFARAGAGEDDEAEPSREPLVVEV
VEHVNLNSSRSDKETIHLALAFEDGAPAYEPGDSLEIFPENDPQLVDEILNAAGLAGDEALRRALLAERDITTLSAATIE
RFVKATGHADAQTLIDSGEAKAWVEGRHLIDLLETYPAKLTAEHIGTITRPLPPRAYSIASSRKEVGDEVHLAIAAVRYE
THGRARSGVASVHVADRIRDGAKLRVRLKPNRHFRLPQDPATDIIMVGPGTGVAPFRAFVQERRAVEAPGRSWLFFGDRH
FTHDFLYQLEWQDALEDGSLTKIDVAFSRDQPEKVYVQDRITQHAQELVSWLDGGAHLYVCGDAKNMAKDVRAAVVKAYE
TAKSLSAADAEAQVAALERSHRYQQDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=606, Percent_Identity=31.023102310231, Blast_Score=232, Evalue=7e-61,
Organism=Homo sapiens, GI10835173, Length=644, Percent_Identity=27.1739130434783, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI24041029, Length=605, Percent_Identity=27.603305785124, Blast_Score=173, Evalue=5e-43,
Organism=Homo sapiens, GI40254422, Length=653, Percent_Identity=26.7993874425727, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI169790956, Length=265, Percent_Identity=35.0943396226415, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI169790958, Length=265, Percent_Identity=35.0943396226415, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI7657393, Length=249, Percent_Identity=35.7429718875502, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI221316705, Length=258, Percent_Identity=34.8837209302326, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI221316709, Length=249, Percent_Identity=35.7429718875502, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI221316707, Length=521, Percent_Identity=26.295585412668, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1789123, Length=593, Percent_Identity=38.2799325463744, Blast_Score=408, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17554134, Length=592, Percent_Identity=29.5608108108108, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17566446, Length=575, Percent_Identity=24.3478260869565, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17531441, Length=260, Percent_Identity=26.5384615384615, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321832, Length=637, Percent_Identity=26.2166405023548, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321143, Length=392, Percent_Identity=29.8469387755102, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325305, Length=633, Percent_Identity=22.2748815165877, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137192, Length=603, Percent_Identity=29.8507462686567, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24582192, Length=516, Percent_Identity=29.6511627906977, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24583543, Length=528, Percent_Identity=30.4924242424242, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI78706876, Length=528, Percent_Identity=30.4924242424242, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI78706872, Length=528, Percent_Identity=30.4924242424242, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24660907, Length=602, Percent_Identity=25.2491694352159, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24660903, Length=602, Percent_Identity=25.2491694352159, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24647438, Length=414, Percent_Identity=22.2222222222222, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 63259; Mature: 63127

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPNLLPRTAPFGDGERASLDAVLGTASPTQRAWLAGFLAGLDAAGGQPAAVPAAPPKA
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AAPLTVIYASESGNSEALAGNVAKLARKQGFKPKVVDFSDLDVATLPKAGKLIAIAATWG
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCHHCCCCCCCEEEEEEECC
EGEPPARAVRAYGELMGEGAPRLDGVEFAVLSLGDTSYAEFCAIGKALDARFEALGAKRA
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AERADLDLDFEKPAADWIKGALKALAPAEQPDNVVAVDFARAGAGEDDEAEPSREPLVVE
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHH
VVEHVNLNSSRSDKETIHLALAFEDGAPAYEPGDSLEIFPENDPQLVDEILNAAGLAGDE
HHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCHH
ALRRALLAERDITTLSAATIERFVKATGHADAQTLIDSGEAKAWVEGRHLIDLLETYPAK
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEECCHHHHHHHHHHCCCH
LTAEHIGTITRPLPPRAYSIASSRKEVGDEVHLAIAAVRYETHGRARSGVASVHVADRIR
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHEEEEEEEEECCCCCCCCCCEEEEHHHHHC
DGAKLRVRLKPNRHFRLPQDPATDIIMVGPGTGVAPFRAFVQERRAVEAPGRSWLFFGDR
CCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
HFTHDFLYQLEWQDALEDGSLTKIDVAFSRDQPEKVYVQDRITQHAQELVSWLDGGAHLY
CCCHHHHEEEEHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEE
VCGDAKNMAKDVRAAVVKAYETAKSLSAADAEAQVAALERSHRYQQDVY
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRPNLLPRTAPFGDGERASLDAVLGTASPTQRAWLAGFLAGLDAAGGQPAAVPAAPPKA
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AAPLTVIYASESGNSEALAGNVAKLARKQGFKPKVVDFSDLDVATLPKAGKLIAIAATWG
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCHHCCCCCCCEEEEEEECC
EGEPPARAVRAYGELMGEGAPRLDGVEFAVLSLGDTSYAEFCAIGKALDARFEALGAKRA
CCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AERADLDLDFEKPAADWIKGALKALAPAEQPDNVVAVDFARAGAGEDDEAEPSREPLVVE
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHH
VVEHVNLNSSRSDKETIHLALAFEDGAPAYEPGDSLEIFPENDPQLVDEILNAAGLAGDE
HHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCHH
ALRRALLAERDITTLSAATIERFVKATGHADAQTLIDSGEAKAWVEGRHLIDLLETYPAK
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEECCHHHHHHHHHHCCCH
LTAEHIGTITRPLPPRAYSIASSRKEVGDEVHLAIAAVRYETHGRARSGVASVHVADRIR
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHEEEEEEEEECCCCCCCCCCEEEEHHHHHC
DGAKLRVRLKPNRHFRLPQDPATDIIMVGPGTGVAPFRAFVQERRAVEAPGRSWLFFGDR
CCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
HFTHDFLYQLEWQDALEDGSLTKIDVAFSRDQPEKVYVQDRITQHAQELVSWLDGGAHLY
CCCHHHHEEEEHHHHCCCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEE
VCGDAKNMAKDVRAAVVKAYETAKSLSAADAEAQVAALERSHRYQQDVY
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]