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The map label for this gene is cysI

Identifier: 170748201

GI number: 170748201

Start: 1900041

End: 1901891

Strand: Reverse

Name: cysI

Synonym: Mrad2831_1783

Alternate gene names: 170748201

Gene position: 1901891-1900041 (Counterclockwise)

Preceding gene: 170748202

Following gene: 170748200

Centisome position: 31.29

GC content: 70.39

Gene sequence:

>1851_bases
ATGGACGACCACAGCCCCCGCGACGCCGCCGAGACCCCGGCCCCCGGCCCTGCCGCCACACCGGCCAAGCGCGTCTACGA
GACCCCGCCGACCTCCCGCCCGATCACGGATGCCGAGGCCGCGCGCGCGGAGAAACTCGCCGCGAACGAGCACATCAAGA
TCGCCAGCGGCTACCTCCGCGGCACGCTGGCGGACGGGCTCCTGAAGCACGCCACCGGGGCGATCTCGGAGGATGACGGG
CAGCTCGTGAAGTTCCACGGGATGTACCTGCAGGACGACCGCGACCTGCGCCCCGAGCGGACCAAGAAGAAGCTCGACAA
GGCCTACAGCTTCATGATCCGCCTGCGCATCGCCGGCGGCGTCATCACCCCGAAGCAGTGGCTGATCCTCGACGACATCG
CCCGGACCTACGCGGGCGGGGCGCTGCGCGCGACCACGCGCCAGACCTTCCAGTACCACGGCGTGATCAAGTCGAACCTG
AAGCGCACGATGGCCGCCATCGACGGCGCGCTGCTCGACACGATCGCGGCCTGCGGCGACGTCAACCGCAACGTCATGGC
GGCGACGAACCCGGCCCAGACCGGCGCCCACAAGGCCGCCTACCAGCTCGCCAAGGACATCTCCGACTCGCTGCTGCCGA
AGACCAGCGCGTGGCGCGAGATCTGGCTCGACGGCGAGCGCGTGGTCGGCGGCGAGGACGAGGCCGAGGTCGAGCCGGTC
TACGGCCGGACCTACCTGCCGCGGAAGTTCAAGACCGTCGTGGCGGTGCCGCCCTCCAACGAGGTCGACATCTTCGCCCA
CGACCTCGGCTTCATCGCGATCCTCGACAAGAAGAACCAGGTCACCGGCTGGAACGTCACGGTGGGCGGCGGCATGGGCA
TGACCCACGGCGAGCCCGACACCTTCCCGCGCACCGCCGACGTGATGGGCTTCGTGAAGCCCGAGGACGCCCTGAAGGTC
GCGGAAGCCGTGATGACGGTCCAGCGCGACTGGGGCAACCGCAAGAACCGCAAGAACGCCCGGCTCAAGTACACGATCGA
GCGCTACGGCCTCGACGCCTTCCGGGCCGAGGTGGAGAAGCGGGTCGGCAAGAAGCTCGGCGCGCCCAAGCCCTTCACCT
TCACGGGCAACGGCGACCGCTACGGCTGGGTCGAGGGCGACGACGGCCGCCACCACCTGACGCTCTACGTGCCCTCGGGC
CGGATCAAGGACATCGACGGCGGCCCGCAATTCCTCTCGGGTCTGCGCCGCATCGCCGAGGTCCACCAGGGCGATTTCCG
CCTGACCGGCAACCAGAACGTGATCATCGCCAACGTCCCGGCCGAGAAGCGCGCCGAGATCGACGCCCTCGTCGATGAGT
ACGGCCTGACCCGGGGCGCGAGCGCGCTCCGGCGCAGCTCGCTCGCCTGCGTGGCCCTGCCGACCTGCGGCCTCGCGCTG
GCCGAGAGCGAGCGCTACCTGCCGGACCTGATGACCGAGCTGGAGGAGAGCCTCGCGTCCCACGGGCTCGCCGAGGAGGA
GATCACGATCCGCTCCACCGGCTGCCCGAACGGATGCGCCCGGCCGTTCATCTCCGAGATCGGCCTCGTGGGCCGCGGCC
CCGAGCGCTATCACCTCTATCTCGGCGCGGCCCACGACGGCTCGCGGCTGAGCAAGCTCTACAAGGAGGACGTGGCGGCC
TCCGAGATCCGCGACACCCTCGACCCGCTGTTCGCCGACTACGCGAGGGGCCGGCAGCCGGGCGAGCATTTCGGCGACTA
CCTGATCCGCGCCGGCCACGTAGCCCGCACCACCAACGGCCCGGACTTCCACGACCGGACCGGCGCCCTCAAGCCGGCCA
CGCTCGGCTGA

Upstream 100 bases:

>100_bases
CCCGAGGTCGCGGATCCGGTTCCAGTCCTGCACCGGCTTCGCTCCGCTCGCCCCCTCCCCACCGGGGAGGAAGACGCGTT
CAGACAGACGGATTCACCCC

Downstream 100 bases:

>100_bases
CGGCAGCCCCCGGGCGCGTCCGGGACGGGGTTCCCGGGCGCGCCGAGGCCGCCGCGGCAGGGCCGCGGATCGCGTGCTCC
GGCCCCGCCTCGCCCTGACC

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MDDHSPRDAAETPAPGPAATPAKRVYETPPTSRPITDAEAARAEKLAANEHIKIASGYLRGTLADGLLKHATGAISEDDG
QLVKFHGMYLQDDRDLRPERTKKKLDKAYSFMIRLRIAGGVITPKQWLILDDIARTYAGGALRATTRQTFQYHGVIKSNL
KRTMAAIDGALLDTIAACGDVNRNVMAATNPAQTGAHKAAYQLAKDISDSLLPKTSAWREIWLDGERVVGGEDEAEVEPV
YGRTYLPRKFKTVVAVPPSNEVDIFAHDLGFIAILDKKNQVTGWNVTVGGGMGMTHGEPDTFPRTADVMGFVKPEDALKV
AEAVMTVQRDWGNRKNRKNARLKYTIERYGLDAFRAEVEKRVGKKLGAPKPFTFTGNGDRYGWVEGDDGRHHLTLYVPSG
RIKDIDGGPQFLSGLRRIAEVHQGDFRLTGNQNVIIANVPAEKRAEIDALVDEYGLTRGASALRRSSLACVALPTCGLAL
AESERYLPDLMTELEESLASHGLAEEEITIRSTGCPNGCARPFISEIGLVGRGPERYHLYLGAAHDGSRLSKLYKEDVAA
SEIRDTLDPLFADYARGRQPGEHFGDYLIRAGHVARTTNGPDFHDRTGALKPATLG

Sequences:

>Translated_616_residues
MDDHSPRDAAETPAPGPAATPAKRVYETPPTSRPITDAEAARAEKLAANEHIKIASGYLRGTLADGLLKHATGAISEDDG
QLVKFHGMYLQDDRDLRPERTKKKLDKAYSFMIRLRIAGGVITPKQWLILDDIARTYAGGALRATTRQTFQYHGVIKSNL
KRTMAAIDGALLDTIAACGDVNRNVMAATNPAQTGAHKAAYQLAKDISDSLLPKTSAWREIWLDGERVVGGEDEAEVEPV
YGRTYLPRKFKTVVAVPPSNEVDIFAHDLGFIAILDKKNQVTGWNVTVGGGMGMTHGEPDTFPRTADVMGFVKPEDALKV
AEAVMTVQRDWGNRKNRKNARLKYTIERYGLDAFRAEVEKRVGKKLGAPKPFTFTGNGDRYGWVEGDDGRHHLTLYVPSG
RIKDIDGGPQFLSGLRRIAEVHQGDFRLTGNQNVIIANVPAEKRAEIDALVDEYGLTRGASALRRSSLACVALPTCGLAL
AESERYLPDLMTELEESLASHGLAEEEITIRSTGCPNGCARPFISEIGLVGRGPERYHLYLGAAHDGSRLSKLYKEDVAA
SEIRDTLDPLFADYARGRQPGEHFGDYLIRAGHVARTTNGPDFHDRTGALKPATLG
>Mature_616_residues
MDDHSPRDAAETPAPGPAATPAKRVYETPPTSRPITDAEAARAEKLAANEHIKIASGYLRGTLADGLLKHATGAISEDDG
QLVKFHGMYLQDDRDLRPERTKKKLDKAYSFMIRLRIAGGVITPKQWLILDDIARTYAGGALRATTRQTFQYHGVIKSNL
KRTMAAIDGALLDTIAACGDVNRNVMAATNPAQTGAHKAAYQLAKDISDSLLPKTSAWREIWLDGERVVGGEDEAEVEPV
YGRTYLPRKFKTVVAVPPSNEVDIFAHDLGFIAILDKKNQVTGWNVTVGGGMGMTHGEPDTFPRTADVMGFVKPEDALKV
AEAVMTVQRDWGNRKNRKNARLKYTIERYGLDAFRAEVEKRVGKKLGAPKPFTFTGNGDRYGWVEGDDGRHHLTLYVPSG
RIKDIDGGPQFLSGLRRIAEVHQGDFRLTGNQNVIIANVPAEKRAEIDALVDEYGLTRGASALRRSSLACVALPTCGLAL
AESERYLPDLMTELEESLASHGLAEEEITIRSTGCPNGCARPFISEIGLVGRGPERYHLYLGAAHDGSRLSKLYKEDVAA
SEIRDTLDPLFADYARGRQPGEHFGDYLIRAGHVARTTNGPDFHDRTGALKPATLG

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=551, Percent_Identity=46.8239564428312, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6322597, Length=591, Percent_Identity=45.3468697123519, Blast_Score=550, Evalue=1e-157,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_METRJ (B1LS97)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754461.1
- ProteinModelPortal:   B1LS97
- SMR:   B1LS97
- GeneID:   6137812
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_1783
- HOGENOM:   HBG626671
- OMA:   TRQAFQM
- ProtClustDB:   PRK13504
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 67333; Mature: 67333

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDHSPRDAAETPAPGPAATPAKRVYETPPTSRPITDAEAARAEKLAANEHIKIASGYLR
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHH
GTLADGLLKHATGAISEDDGQLVKFHGMYLQDDRDLRPERTKKKLDKAYSFMIRLRIAGG
HHHHHHHHHHHCCCCCCCCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCC
VITPKQWLILDDIARTYAGGALRATTRQTFQYHGVIKSNLKRTMAAIDGALLDTIAACGD
CCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VNRNVMAATNPAQTGAHKAAYQLAKDISDSLLPKTSAWREIWLDGERVVGGEDEAEVEPV
CCCCEEEECCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHEECCCEEECCCCCCCCCCC
YGRTYLPRKFKTVVAVPPSNEVDIFAHDLGFIAILDKKNQVTGWNVTVGGGMGMTHGEPD
CCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCCCC
TFPRTADVMGFVKPEDALKVAEAVMTVQRDWGNRKNRKNARLKYTIERYGLDAFRAEVEK
CCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCHHHHHHHHHH
RVGKKLGAPKPFTFTGNGDRYGWVEGDDGRHHLTLYVPSGRIKDIDGGPQFLSGLRRIAE
HHHHHCCCCCCEEECCCCCEECCCCCCCCCEEEEEEECCCCEECCCCCHHHHHHHHHHHH
VHQGDFRLTGNQNVIIANVPAEKRAEIDALVDEYGLTRGASALRRSSLACVALPTCGLAL
HHCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCHH
AESERYLPDLMTELEESLASHGLAEEEITIRSTGCPNGCARPFISEIGLVGRGPERYHLY
HCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEE
LGAAHDGSRLSKLYKEDVAASEIRDTLDPLFADYARGRQPGEHFGDYLIRAGHVARTTNG
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCC
PDFHDRTGALKPATLG
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDDHSPRDAAETPAPGPAATPAKRVYETPPTSRPITDAEAARAEKLAANEHIKIASGYLR
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHH
GTLADGLLKHATGAISEDDGQLVKFHGMYLQDDRDLRPERTKKKLDKAYSFMIRLRIAGG
HHHHHHHHHHHCCCCCCCCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHEEEEECCC
VITPKQWLILDDIARTYAGGALRATTRQTFQYHGVIKSNLKRTMAAIDGALLDTIAACGD
CCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VNRNVMAATNPAQTGAHKAAYQLAKDISDSLLPKTSAWREIWLDGERVVGGEDEAEVEPV
CCCCEEEECCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHEECCCEEECCCCCCCCCCC
YGRTYLPRKFKTVVAVPPSNEVDIFAHDLGFIAILDKKNQVTGWNVTVGGGMGMTHGEPD
CCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCCCC
TFPRTADVMGFVKPEDALKVAEAVMTVQRDWGNRKNRKNARLKYTIERYGLDAFRAEVEK
CCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCHHHHHHHHHH
RVGKKLGAPKPFTFTGNGDRYGWVEGDDGRHHLTLYVPSGRIKDIDGGPQFLSGLRRIAE
HHHHHCCCCCCEEECCCCCEECCCCCCCCCEEEEEEECCCCEECCCCCHHHHHHHHHHHH
VHQGDFRLTGNQNVIIANVPAEKRAEIDALVDEYGLTRGASALRRSSLACVALPTCGLAL
HHCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCHH
AESERYLPDLMTELEESLASHGLAEEEITIRSTGCPNGCARPFISEIGLVGRGPERYHLY
HCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEE
LGAAHDGSRLSKLYKEDVAASEIRDTLDPLFADYARGRQPGEHFGDYLIRAGHVARTTNG
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCC
PDFHDRTGALKPATLG
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA