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The map label for this gene is rutB

Identifier: 170748001

GI number: 170748001

Start: 1671880

End: 1672656

Strand: Reverse

Name: rutB

Synonym: Mrad2831_1583

Alternate gene names: 170748001

Gene position: 1672656-1671880 (Counterclockwise)

Preceding gene: 170748002

Following gene: 170748000

Centisome position: 27.52

GC content: 71.81

Gene sequence:

>777_bases
ATGGACCGCCCGACGACCTATCCGATGGACCAGCCCGCCGGCTTCCGCGACGCCCAGGGTCGGCACGGCGGCGTCGTCCT
GCCGGCCCGCCCGGAGCCGATCGCCCTCGATCCCGCGACCACGTTCCTCATCGTCGTGGACGTCCAGAACGCCTACGCGA
GCCCGGGCGGCTACCTCGACCGGGCCGGCTTCGACGTCTCGGGCACCGGCCCGGTGATCGCCCGGATCGCCCGGGCGGTG
GCGGCGGCGCGCGCCGCCGGCATCCCGGTCCTGTGGTTCCAGAACGGCTGGGACCCCGCCTATGTCGAGGCCGGCGGCCC
GGGCTCGCCGAACTGGCACAAATCCAACGCCCTCAAGACCATGCGTCGGAATCCTGAAGCGAACACGCAGCTCCTCGCCA
AGGGATCCTGGGACTACGCGCTGGTGGATGCCCTGACGCCGGAGCCCGGCGACATCGTGATGGGCAAGCCCCGCTACAGC
GGCTTCTACAACACGCCCCTCGACAGCACCCTGCGGGCCCGGGGCGTGACCACGCTGGTCTTCACCGGCATCGCCACCAA
CGTCTGCGTCGAGTCGACCCTGCGGGACGGGTTCCACCGGGAGTATTTCGGGATCGTGCTGGCCGACGCCACGCACCAGG
CCGGACCGGAGAGCCTGCACCGCGCGGCGCTCGCCAATATCGAGACCTTCTTCGGCTGGGTCTCGGACGTCGCCGCCTTC
GAGTCCGCCCTCGGGGTGCCCTCGCGCGCCCCGGCCGGCGCCGATCTGGCCGGATAA

Upstream 100 bases:

>100_bases
ACGATTTCCTCAAGGGCCTCGACGCCTTCGGGACCCGGATCCAGCCCCTGATGCGGTCGCGCCGGCACGTGACGGCGGCA
GCTCTGCCCGAGGTCGCCTG

Downstream 100 bases:

>100_bases
GTGCATCGCCCGCCCGGCCGGTGACCGGCAGGGTGCGCGAGGACCGCCGCGACGCGGCCCTCCGCGCGGCCTGCCCGGCC
CGGCCGACAGGTCGAGCGCG

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MDRPTTYPMDQPAGFRDAQGRHGGVVLPARPEPIALDPATTFLIVVDVQNAYASPGGYLDRAGFDVSGTGPVIARIARAV
AAARAAGIPVLWFQNGWDPAYVEAGGPGSPNWHKSNALKTMRRNPEANTQLLAKGSWDYALVDALTPEPGDIVMGKPRYS
GFYNTPLDSTLRARGVTTLVFTGIATNVCVESTLRDGFHREYFGIVLADATHQAGPESLHRAALANIETFFGWVSDVAAF
ESALGVPSRAPAGADLAG

Sequences:

>Translated_258_residues
MDRPTTYPMDQPAGFRDAQGRHGGVVLPARPEPIALDPATTFLIVVDVQNAYASPGGYLDRAGFDVSGTGPVIARIARAV
AAARAAGIPVLWFQNGWDPAYVEAGGPGSPNWHKSNALKTMRRNPEANTQLLAKGSWDYALVDALTPEPGDIVMGKPRYS
GFYNTPLDSTLRARGVTTLVFTGIATNVCVESTLRDGFHREYFGIVLADATHQAGPESLHRAALANIETFFGWVSDVAAF
ESALGVPSRAPAGADLAG
>Mature_258_residues
MDRPTTYPMDQPAGFRDAQGRHGGVVLPARPEPIALDPATTFLIVVDVQNAYASPGGYLDRAGFDVSGTGPVIARIARAV
AAARAAGIPVLWFQNGWDPAYVEAGGPGSPNWHKSNALKTMRRNPEANTQLLAKGSWDYALVDALTPEPGDIVMGKPRYS
GFYNTPLDSTLRARGVTTLVFTGIATNVCVESTLRDGFHREYFGIVLADATHQAGPESLHRAALANIETFFGWVSDVAAF
ESALGVPSRAPAGADLAG

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily

Homologues:

Organism=Escherichia coli, GI87081820, Length=224, Percent_Identity=72.7678571428571, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI87081992, Length=216, Percent_Identity=30.0925925925926, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTB_METRJ (B1M6B8)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754261.1
- ProteinModelPortal:   B1M6B8
- SMR:   B1M6B8
- GeneID:   6137611
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_1583
- HOGENOM:   HBG752434
- OMA:   MQNAYAS
- ProtClustDB:   CLSK879924
- HAMAP:   MF_00830
- InterPro:   IPR019916
- InterPro:   IPR000868
- Gene3D:   G3DSA:3.40.50.850
- TIGRFAMs:   TIGR03614

Pfam domain/function: PF00857 Isochorismatase; SSF52499 Iscrsm_hydrolase

EC number: NA

Molecular weight: Translated: 27350; Mature: 27350

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: ACT_SITE 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPTTYPMDQPAGFRDAQGRHGGVVLPARPEPIALDPATTFLIVVDVQNAYASPGGYLD
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCEEEEEEEECCCCCCCCCCCC
RAGFDVSGTGPVIARIARAVAAARAAGIPVLWFQNGWDPAYVEAGGPGSPNWHKSNALKT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHH
MRRNPEANTQLLAKGSWDYALVDALTPEPGDIVMGKPRYSGFYNTPLDSTLRARGVTTLV
HHCCCCCCCEEEECCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCHHHHHCCCEEEE
FTGIATNVCVESTLRDGFHREYFGIVLADATHQAGPESLHRAALANIETFFGWVSDVAAF
EHHHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ESALGVPSRAPAGADLAG
HHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDRPTTYPMDQPAGFRDAQGRHGGVVLPARPEPIALDPATTFLIVVDVQNAYASPGGYLD
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEECCCCEEEEEEEECCCCCCCCCCCC
RAGFDVSGTGPVIARIARAVAAARAAGIPVLWFQNGWDPAYVEAGGPGSPNWHKSNALKT
CCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHH
MRRNPEANTQLLAKGSWDYALVDALTPEPGDIVMGKPRYSGFYNTPLDSTLRARGVTTLV
HHCCCCCCCEEEECCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCCCHHHHHCCCEEEE
FTGIATNVCVESTLRDGFHREYFGIVLADATHQAGPESLHRAALANIETFFGWVSDVAAF
EHHHHHHHHHHHHHHHCHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ESALGVPSRAPAGADLAG
HHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA