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The map label for this gene is rutA

Identifier: 170748002

GI number: 170748002

Start: 1672656

End: 1673801

Strand: Reverse

Name: rutA

Synonym: Mrad2831_1584

Alternate gene names: 170748002

Gene position: 1673801-1672656 (Counterclockwise)

Preceding gene: 170748005

Following gene: 170748001

Centisome position: 27.54

GC content: 69.72

Gene sequence:

>1146_bases
ATGATGCGAAGCCACGCGGACGACAGCGCCGAGGCCCGTCCGGCGCCGGCCATGAGCGTCGGGGTGTTCATCCCGATCGG
CAACAACGGCTGGCTGCTGTCGGAGAACGCCCCGCAGTACCGCCCGAGCTTCGCGCTCAACAAGGCGATCACCCTGAAGG
CGGAGGGCTACGGGCTCGACTTCGCCCTGTCGATGATCAAGCTCCGCGGCTTCGGCGGCAAGACCGAGTTCTGGGACCAC
AACCTCGAATCCTTCACCCTGATGGCGGGGCTCGCCGCCGTCACCACCCGGATCCGGCTGTTCGCCACCGCGGCGACCCT
GTGCCTGCCCCCCGCCATCGTGGCCCGCATGGCGGCGACCATCGACTCGATCTCCGACGGGCGCTTCGGCCTGAACCTCG
TCACCGGCTGGCAGAAGCCGGAATACGACCAGATGGGCCTGTGGCCGGGCGACGCGCATTTTGCCAATCGCTACGATTAC
CTGGCCGAGTACGCGCAGATCCTGCGCGACCTGTGGGAGACGGGCGCCAGCGACCGCAAGGGCGCGTATTTCCAGATGAA
CGATTGCCGGCTGAGCCCCCGGCCGCAGGCGCCGGTGAAGATCATCTGCGCCGGCCAGAGCGGCGCCGGGCTGGCCTTCA
CGGCCCAGTACGCCGACTACAATTTCTGCCTGGGCAAGGGGCTCAACACGCCGACCGCCTTCGCGCCCGTGGTCGAGAAG
CTCGCCGAGGCCAGCGCGCGGACCGGGCGGCGGGTGACCGCCTTCGCGCTGTTCATGATCATCGCCGACGAGACCGACGA
CGCCGCCATGGCGACCTGGGCGCATTACAGGGCGGGCGCGGATGCCGAGGCGATCGCGTGGCTCGGCGCGCAGGGCGCGG
CCGACACCAGGTCCGGCAGCGACACCAACGTGCGCCAGCTCGCCGACCCGGCCTCGGCGGTGAACCTCAACATGGGCACC
CTGGTGGGCAGCTACGCCAGCGTGGCCGCGATGCTCGACGCCGTCATGACCATCGACGGGGTGGAGGGCGTGCTGCTGGT
CTTCGACGATTTCCTCAAGGGCCTCGACGCCTTCGGGACCCGGATCCAGCCCCTGATGCGGTCGCGCCGGCACGTGACGG
CGGCAGCTCTGCCCGAGGTCGCCTGA

Upstream 100 bases:

>100_bases
CCCGTCGGGCGCCGCGGGTTTGACCCTCCGCCGCGGTTTTGCGAGCCTCCGTGGGCCGACTCGGTTCCGGCGGGGAACCG
ACCGGGCGGAGCGCGGGACG

Downstream 100 bases:

>100_bases
TGGACCGCCCGACGACCTATCCGATGGACCAGCCCGCCGGCTTCCGCGACGCCCAGGGTCGGCACGGCGGCGTCGTCCTG
CCGGCCCGCCCGGAGCCGAT

Product: luciferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MMRSHADDSAEARPAPAMSVGVFIPIGNNGWLLSENAPQYRPSFALNKAITLKAEGYGLDFALSMIKLRGFGGKTEFWDH
NLESFTLMAGLAAVTTRIRLFATAATLCLPPAIVARMAATIDSISDGRFGLNLVTGWQKPEYDQMGLWPGDAHFANRYDY
LAEYAQILRDLWETGASDRKGAYFQMNDCRLSPRPQAPVKIICAGQSGAGLAFTAQYADYNFCLGKGLNTPTAFAPVVEK
LAEASARTGRRVTAFALFMIIADETDDAAMATWAHYRAGADAEAIAWLGAQGAADTRSGSDTNVRQLADPASAVNLNMGT
LVGSYASVAAMLDAVMTIDGVEGVLLVFDDFLKGLDAFGTRIQPLMRSRRHVTAAALPEVA

Sequences:

>Translated_381_residues
MMRSHADDSAEARPAPAMSVGVFIPIGNNGWLLSENAPQYRPSFALNKAITLKAEGYGLDFALSMIKLRGFGGKTEFWDH
NLESFTLMAGLAAVTTRIRLFATAATLCLPPAIVARMAATIDSISDGRFGLNLVTGWQKPEYDQMGLWPGDAHFANRYDY
LAEYAQILRDLWETGASDRKGAYFQMNDCRLSPRPQAPVKIICAGQSGAGLAFTAQYADYNFCLGKGLNTPTAFAPVVEK
LAEASARTGRRVTAFALFMIIADETDDAAMATWAHYRAGADAEAIAWLGAQGAADTRSGSDTNVRQLADPASAVNLNMGT
LVGSYASVAAMLDAVMTIDGVEGVLLVFDDFLKGLDAFGTRIQPLMRSRRHVTAAALPEVA
>Mature_381_residues
MMRSHADDSAEARPAPAMSVGVFIPIGNNGWLLSENAPQYRPSFALNKAITLKAEGYGLDFALSMIKLRGFGGKTEFWDH
NLESFTLMAGLAAVTTRIRLFATAATLCLPPAIVARMAATIDSISDGRFGLNLVTGWQKPEYDQMGLWPGDAHFANRYDY
LAEYAQILRDLWETGASDRKGAYFQMNDCRLSPRPQAPVKIICAGQSGAGLAFTAQYADYNFCLGKGLNTPTAFAPVVEK
LAEASARTGRRVTAFALFMIIADETDDAAMATWAHYRAGADAEAIAWLGAQGAADTRSGSDTNVRQLADPASAVNLNMGT
LVGSYASVAAMLDAVMTIDGVEGVLLVFDDFLKGLDAFGTRIQPLMRSRRHVTAAALPEVA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily

Homologues:

Organism=Escherichia coli, GI1787247, Length=357, Percent_Identity=72.2689075630252, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1787166, Length=314, Percent_Identity=24.203821656051, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTA_METRJ (B1M6B9)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754262.1
- GeneID:   6137612
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_1584
- HOGENOM:   HBG350183
- OMA:   FIPIGNN
- ProtClustDB:   CLSK879925
- HAMAP:   MF_01699
- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048
- Gene3D:   G3DSA:3.20.20.30
- TIGRFAMs:   TIGR03612

Pfam domain/function: PF00296 Bac_luciferase; SSF51679 Luciferase_like

EC number: NA

Molecular weight: Translated: 40926; Mature: 40926

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: BINDING 132-132 BINDING 141-141 BINDING 207-207

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRSHADDSAEARPAPAMSVGVFIPIGNNGWLLSENAPQYRPSFALNKAITLKAEGYGLD
CCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCCCCCCCEECCCEEEEEECCCCHH
FALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTTRIRLFATAATLCLPPAIVARMAAT
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IDSISDGRFGLNLVTGWQKPEYDQMGLWPGDAHFANRYDYLAEYAQILRDLWETGASDRK
HHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAYFQMNDCRLSPRPQAPVKIICAGQSGAGLAFTAQYADYNFCLGKGLNTPTAFAPVVEK
CCEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHH
LAEASARTGRRVTAFALFMIIADETDDAAMATWAHYRAGADAEAIAWLGAQGAADTRSGS
HHHHHHHCCCHHHHEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHEEECCCCCCCCCCCC
DTNVRQLADPASAVNLNMGTLVGSYASVAAMLDAVMTIDGVEGVLLVFDDFLKGLDAFGT
CCCHHHHCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RIQPLMRSRRHVTAAALPEVA
HHHHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MMRSHADDSAEARPAPAMSVGVFIPIGNNGWLLSENAPQYRPSFALNKAITLKAEGYGLD
CCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEECCCCCCCCCCEECCCEEEEEECCCCHH
FALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTTRIRLFATAATLCLPPAIVARMAAT
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IDSISDGRFGLNLVTGWQKPEYDQMGLWPGDAHFANRYDYLAEYAQILRDLWETGASDRK
HHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GAYFQMNDCRLSPRPQAPVKIICAGQSGAGLAFTAQYADYNFCLGKGLNTPTAFAPVVEK
CCEEEECCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHH
LAEASARTGRRVTAFALFMIIADETDDAAMATWAHYRAGADAEAIAWLGAQGAADTRSGS
HHHHHHHCCCHHHHEEEEEEEECCCCCHHHHHHHHHHCCCCHHHHEEECCCCCCCCCCCC
DTNVRQLADPASAVNLNMGTLVGSYASVAAMLDAVMTIDGVEGVLLVFDDFLKGLDAFGT
CCCHHHHCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RIQPLMRSRRHVTAAALPEVA
HHHHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA