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The map label for this gene is nodW [H]

Identifier: 170747693

GI number: 170747693

Start: 1328996

End: 1329658

Strand: Reverse

Name: nodW [H]

Synonym: Mrad2831_1268

Alternate gene names: 170747693

Gene position: 1329658-1328996 (Counterclockwise)

Preceding gene: 170747695

Following gene: 170747692

Centisome position: 21.88

GC content: 70.14

Gene sequence:

>663_bases
ATGTCAGGCAACGTCCGGGGTGGCGCGGCGAAAGCCGGCGCCGACACGGCCTCACCGCTGGTTGTCATCGTCGAGGACGA
CGAGGGCGTCCGGGAGGGGCTCCAGGATCTCCTGCGCTCCGTCGGCCTCGACACCCTCGCCTACGGCGCGACGAGCGAGC
TCCTCGCGGCGACGCTGCCGGACCGCCCGGGCTGCCTGATCCTCGATGTGCGGCTGCCGGGATCCAGCGGTCTCGACCTG
CAGGCGAAGCTGGCCGCGATGGGCAACCGCATGCCGATCATCTTCATGACCGGCCACGGCGACATCCCCATGACCGTCCA
GGCCATGAAGGCGGGCGCGCTCGACTTCCTGACCAAGCCGTTCCGCGACCAGGACATGCTCGACGCCATCGCGGTCGCGA
TCGAGCGGGACAGGACCCGGCGCGCCTCGAGCGCGGGGGTCGCCGAACTCGAGGCGCTGGCCGCGACCCTGACGGCGCGG
GAGGCCGAGGTGATGGCGCACGTCGTCAAGGGGCTGCTCAACAAGCAGATCGCCTACGCCCTCGGCATCAGCGAGATCAC
AGTCAAGATCCACCGCGGCAACGTCATGCGGAAGATGGAGGCCGGCTCCGTGGCCGACCTCGTGCGCAAGACCGAGCTGC
TCAAGGCCCGGGCGACCGTCTGA

Upstream 100 bases:

>100_bases
GCCGTCCGAAGACGGTCGGCTTGATCGGGCGCGGATTCAACCTGTAAGCACTGTCCTCGCGGACAGCCTGGCAGGGGCGC
GCGGGGGCTCGTCGATCACG

Downstream 100 bases:

>100_bases
GCCCTAAGCCCCCCGCAGTCGCCGGCCCGGGCCGGGCCGATCGGCGCGGAACGATGCCGGGCATCACCGCGTTGGCACCC
GCACGGGACAGATTTCGTCG

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSGNVRGGAAKAGADTASPLVVIVEDDEGVREGLQDLLRSVGLDTLAYGATSELLAATLPDRPGCLILDVRLPGSSGLDL
QAKLAAMGNRMPIIFMTGHGDIPMTVQAMKAGALDFLTKPFRDQDMLDAIAVAIERDRTRRASSAGVAELEALAATLTAR
EAEVMAHVVKGLLNKQIAYALGISEITVKIHRGNVMRKMEAGSVADLVRKTELLKARATV

Sequences:

>Translated_220_residues
MSGNVRGGAAKAGADTASPLVVIVEDDEGVREGLQDLLRSVGLDTLAYGATSELLAATLPDRPGCLILDVRLPGSSGLDL
QAKLAAMGNRMPIIFMTGHGDIPMTVQAMKAGALDFLTKPFRDQDMLDAIAVAIERDRTRRASSAGVAELEALAATLTAR
EAEVMAHVVKGLLNKQIAYALGISEITVKIHRGNVMRKMEAGSVADLVRKTELLKARATV
>Mature_219_residues
SGNVRGGAAKAGADTASPLVVIVEDDEGVREGLQDLLRSVGLDTLAYGATSELLAATLPDRPGCLILDVRLPGSSGLDLQ
AKLAAMGNRMPIIFMTGHGDIPMTVQAMKAGALDFLTKPFRDQDMLDAIAVAIERDRTRRASSAGVAELEALAATLTARE
AEVMAHVVKGLLNKQIAYALGISEITVKIHRGNVMRKMEAGSVADLVRKTELLKARATV

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=35.042735042735, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786747, Length=207, Percent_Identity=23.1884057971014, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=29.0816326530612, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=30.7692307692308, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23202; Mature: 23071

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNVRGGAAKAGADTASPLVVIVEDDEGVREGLQDLLRSVGLDTLAYGATSELLAATLP
CCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCC
DRPGCLILDVRLPGSSGLDLQAKLAAMGNRMPIIFMTGHGDIPMTVQAMKAGALDFLTKP
CCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHCCHHHHHHCC
FRDQDMLDAIAVAIERDRTRRASSAGVAELEALAATLTAREAEVMAHVVKGLLNKQIAYA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGISEITVKIHRGNVMRKMEAGSVADLVRKTELLKARATV
CCHHHEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGNVRGGAAKAGADTASPLVVIVEDDEGVREGLQDLLRSVGLDTLAYGATSELLAATLP
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCC
DRPGCLILDVRLPGSSGLDLQAKLAAMGNRMPIIFMTGHGDIPMTVQAMKAGALDFLTKP
CCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHCCHHHHHHCC
FRDQDMLDAIAVAIERDRTRRASSAGVAELEALAATLTAREAEVMAHVVKGLLNKQIAYA
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGISEITVKIHRGNVMRKMEAGSVADLVRKTELLKARATV
CCHHHEEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]