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The map label for this gene is fixJ [H]

Identifier: 170747692

GI number: 170747692

Start: 1328297

End: 1328668

Strand: Reverse

Name: fixJ [H]

Synonym: Mrad2831_1267

Alternate gene names: 170747692

Gene position: 1328668-1328297 (Counterclockwise)

Preceding gene: 170747693

Following gene: 170747691

Centisome position: 21.86

GC content: 70.16

Gene sequence:

>372_bases
GTGCCGGTCGGGAAGAAAGCGGTCGCGGTCGTCGACGACGACGAGGCGGTTCTGGACGCGACGTCCAGCTTCCTCGAAGC
GTTGGGCTACGACGCGCGGGCCTTCAACTCGGGCGAGGCCTTCCTGGCCTCCATGGCCGGCAGCGACGTCTCGTGCCTGC
TGACGGACGTGAACATGCCGGGGATCAGCGGCATCGAGTTGCAGGACCGCGTCCGGCAGCAGCGCCCTGCCCTGCCGGTC
ATCATGATGACGGCGCTGACCGACGACGCGCTCCGGCGTCGGGCCCTCGCCGGCGGCGCCCGCGATCTCCTGCGCAAGCC
CCTCGTGGCCGACGAACTGATCCGCTGCCTGGAAGAGACGATCGGGGCCTGA

Upstream 100 bases:

>100_bases
TCCGTCGCGCGCATCAAACCATCGTATAATATCGTCGGCGCGGCCGCTGGCCCACACAGGTGGGATCGACGCGCCTCCGA
GAGGCTCCGGAGGGAGATTC

Downstream 100 bases:

>100_bases
GGATCGGCCGCGTGAACGTCGGCCAGGCTGCGCGCGGCCGTCACGCGCCTGCGCGCCCGGACGATCCGATTAGGACATCA
AACCTCCGTATGATTCCGGC

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MPVGKKAVAVVDDDEAVLDATSSFLEALGYDARAFNSGEAFLASMAGSDVSCLLTDVNMPGISGIELQDRVRQQRPALPV
IMMTALTDDALRRRALAGGARDLLRKPLVADELIRCLEETIGA

Sequences:

>Translated_123_residues
MPVGKKAVAVVDDDEAVLDATSSFLEALGYDARAFNSGEAFLASMAGSDVSCLLTDVNMPGISGIELQDRVRQQRPALPV
IMMTALTDDALRRRALAGGARDLLRKPLVADELIRCLEETIGA
>Mature_122_residues
PVGKKAVAVVDDDEAVLDATSSFLEALGYDARAFNSGEAFLASMAGSDVSCLLTDVNMPGISGIELQDRVRQQRPALPVI
MMTALTDDALRRRALAGGARDLLRKPLVADELIRCLEETIGA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=114, Percent_Identity=35.9649122807018, Blast_Score=69, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13090; Mature: 12959

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVGKKAVAVVDDDEAVLDATSSFLEALGYDARAFNSGEAFLASMAGSDVSCLLTDVNMP
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCC
GISGIELQDRVRQQRPALPVIMMTALTDDALRRRALAGGARDLLRKPLVADELIRCLEET
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHH
IGA
HCC
>Mature Secondary Structure 
PVGKKAVAVVDDDEAVLDATSSFLEALGYDARAFNSGEAFLASMAGSDVSCLLTDVNMP
CCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCC
GISGIELQDRVRQQRPALPVIMMTALTDDALRRRALAGGARDLLRKPLVADELIRCLEET
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHH
IGA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]