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The map label for this gene is gltR [H]

Identifier: 170747513

GI number: 170747513

Start: 1139267

End: 1140169

Strand: Reverse

Name: gltR [H]

Synonym: Mrad2831_1081

Alternate gene names: 170747513

Gene position: 1140169-1139267 (Counterclockwise)

Preceding gene: 170747519

Following gene: 170747512

Centisome position: 18.76

GC content: 76.52

Gene sequence:

>903_bases
ATGAACAGCACCGACCTGATGTTCTTCGCGGCGGTGGCCGAGACCGGCGGCATCGGGCGGGCGGCGGCACGGCTCAACAC
CGTCCAGTCCAACGTCACCGGCCGGATCCGCGCCCTGGAGCAGGCCCTGCGCGTGCCGCTGTTCCACCGCGGCAGCCGCG
GGGTGACGCTGACCCGGGCCGGGGAGCGGCTCCTGCCCTACGCCCTCCAGGTCGGGCAGCTGCTCGCGGAGGCGCGGCAC
GCCCTGCTGGCCGAGGGCGAACCCCGCGGACCGCTGCGGATCGGCGCCATGGAGACGACCGCGGCCCTGCGCCTGCCCGG
CCCGCTCGCCGACTTCGCCGCGCGCCACCCGGACGTGGACATCGAGCTGGAGACCGGCCCGACCGAGCGGCTGATCGCCC
GGGTCCTCGACCGGAGCCTGGAGGGGGCCTTCGTGGCCGGCCCGGTCGCGCAGGCCGACCTCGTGGGGATCCCGGTCTTC
ACGGAGGAGCTGGTGCTGGTGGCGCACCCGGCCGTCGGCGACCTCGCGGCGCTGCTCGACCGGCTGGCCCGGGCGCGCGA
CGCGAAGGTGCTCGTGTTCCGGGCCGGCTGCTCGTACCGGAGGCGCCTGGAGGATTTTCTGGCCCGCCAGGGCCTCGTGC
ACGTGCGGCGCATGGAGTTCGGCACCCTCGACGGCATCCTCGGCTGCGTGGCGGCCGGCCTGGGCGTGACCCTGGTTCCG
CGGGGCGTGGCGGACCCGGCGCACCGCGCCGGCCGCGTCTCGCTCCACCCCGTGCCCGACGGGGACGGGCACGCCCCGAC
CCTGCTGGTCCACCGCGCCGACGTCCGGCCGAGCGCGGCCTTCGCGCGGTTCGCCGAGCAGGTCCGGGTGGCCTTCCCGG
ATTCGCCGTCGGATTCGCCCTAG

Upstream 100 bases:

>100_bases
CGGTCGCGCGGTTGCGCGCGCCGCCCGTCCGGCCTTAACGATCCGTACGATGCGGAAAAAATGATTCCTTCAGAAGGAAG
GCATCGGAAAAGCAGATGGC

Downstream 100 bases:

>100_bases
GGCCTCGGTTCCGGCACGGACCGTGCCCTTGACAACCGCCGGTTGACAGCGGCCCGAGCCCGGGCGCTTCCTCCCGGCCG
CAGACGGTTCGGAGTTCCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNSTDLMFFAAVAETGGIGRAAARLNTVQSNVTGRIRALEQALRVPLFHRGSRGVTLTRAGERLLPYALQVGQLLAEARH
ALLAEGEPRGPLRIGAMETTAALRLPGPLADFAARHPDVDIELETGPTERLIARVLDRSLEGAFVAGPVAQADLVGIPVF
TEELVLVAHPAVGDLAALLDRLARARDAKVLVFRAGCSYRRRLEDFLARQGLVHVRRMEFGTLDGILGCVAAGLGVTLVP
RGVADPAHRAGRVSLHPVPDGDGHAPTLLVHRADVRPSAAFARFAEQVRVAFPDSPSDSP

Sequences:

>Translated_300_residues
MNSTDLMFFAAVAETGGIGRAAARLNTVQSNVTGRIRALEQALRVPLFHRGSRGVTLTRAGERLLPYALQVGQLLAEARH
ALLAEGEPRGPLRIGAMETTAALRLPGPLADFAARHPDVDIELETGPTERLIARVLDRSLEGAFVAGPVAQADLVGIPVF
TEELVLVAHPAVGDLAALLDRLARARDAKVLVFRAGCSYRRRLEDFLARQGLVHVRRMEFGTLDGILGCVAAGLGVTLVP
RGVADPAHRAGRVSLHPVPDGDGHAPTLLVHRADVRPSAAFARFAEQVRVAFPDSPSDSP
>Mature_300_residues
MNSTDLMFFAAVAETGGIGRAAARLNTVQSNVTGRIRALEQALRVPLFHRGSRGVTLTRAGERLLPYALQVGQLLAEARH
ALLAEGEPRGPLRIGAMETTAALRLPGPLADFAARHPDVDIELETGPTERLIARVLDRSLEGAFVAGPVAQADLVGIPVF
TEELVLVAHPAVGDLAALLDRLARARDAKVLVFRAGCSYRRRLEDFLARQGLVHVRRMEFGTLDGILGCVAAGLGVTLVP
RGVADPAHRAGRVSLHPVPDGDGHAPTLLVHRADVRPSAAFARFAEQVRVAFPDSPSDSP

Specific function: Positive regulator of glutamate biosynthesis (gltAB genes). Negatively regulates its own expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=299, Percent_Identity=36.7892976588629, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI157672245, Length=262, Percent_Identity=30.9160305343511, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=27.5510204081633, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788481, Length=284, Percent_Identity=29.2253521126761, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693105, Length=242, Percent_Identity=27.6859504132231, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790399, Length=293, Percent_Identity=26.6211604095563, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787879, Length=244, Percent_Identity=31.1475409836066, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790208, Length=252, Percent_Identity=29.3650793650794, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786401, Length=171, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787601, Length=295, Percent_Identity=25.7627118644068, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788887, Length=273, Percent_Identity=29.3040293040293, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787128, Length=163, Percent_Identity=28.2208588957055, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI87081904, Length=300, Percent_Identity=26.3333333333333, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788748, Length=251, Percent_Identity=28.6852589641434, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=35.7723577235772, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31952; Mature: 31952

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTDLMFFAAVAETGGIGRAAARLNTVQSNVTGRIRALEQALRVPLFHRGSRGVTLTRA
CCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECC
GERLLPYALQVGQLLAEARHALLAEGEPRGPLRIGAMETTAALRLPGPLADFAARHPDVD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCCCHHHHHHCCCCCE
IELETGPTERLIARVLDRSLEGAFVAGPVAQADLVGIPVFTEELVLVAHPAVGDLAALLD
EEECCCCHHHHHHHHHHHCCCCCEEECCCCCCCEEECCHHHHCEEEEECCCHHHHHHHHH
RLARARDAKVLVFRAGCSYRRRLEDFLARQGLVHVRRMEFGTLDGILGCVAAGLGVTLVP
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCEEEC
RGVADPAHRAGRVSLHPVPDGDGHAPTLLVHRADVRPSAAFARFAEQVRVAFPDSPSDSP
CCCCCHHHHCCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHEEECCCCCCCCC
>Mature Secondary Structure
MNSTDLMFFAAVAETGGIGRAAARLNTVQSNVTGRIRALEQALRVPLFHRGSRGVTLTRA
CCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECC
GERLLPYALQVGQLLAEARHALLAEGEPRGPLRIGAMETTAALRLPGPLADFAARHPDVD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCCCHHHHHHCCCCCE
IELETGPTERLIARVLDRSLEGAFVAGPVAQADLVGIPVFTEELVLVAHPAVGDLAALLD
EEECCCCHHHHHHHHHHHCCCCCEEECCCCCCCEEECCHHHHCEEEEECCCHHHHHHHHH
RLARARDAKVLVFRAGCSYRRRLEDFLARQGLVHVRRMEFGTLDGILGCVAAGLGVTLVP
HHHHCCCCEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCEEEC
RGVADPAHRAGRVSLHPVPDGDGHAPTLLVHRADVRPSAAFARFAEQVRVAFPDSPSDSP
CCCCCHHHHCCCEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]