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The map label for this gene is yafC [H]

Identifier: 170747519

GI number: 170747519

Start: 1143856

End: 1144806

Strand: Reverse

Name: yafC [H]

Synonym: Mrad2831_1087

Alternate gene names: 170747519

Gene position: 1144806-1143856 (Counterclockwise)

Preceding gene: 170747521

Following gene: 170747513

Centisome position: 18.84

GC content: 75.18

Gene sequence:

>951_bases
ATGGATAGATCCGACGTCACCCTGGAGCGCATGCGCAGCTTCGTCCGGGTGGCCGAGCGCGGCAGCCTGTCGGCGGTGGC
GCGCGAGTTCGGGGTCGGGCAATCGACGATCACCCGGCACCTGCGCGAACTCGAGGACGCCCTCGGCGTCGCCCTGATCA
GCCGGACGACGCGGCGCCTCGCCCTGACGGCCGAGGGCTTGGCCTATTACGAGAACAGCCTGCGCATCCTGCACCTCGTC
GAGCAGGCGGGCGCCGAGGCGCGGGACCGGCGGCACGCCCCGGCGGGCACCGTCCGCCTGTCGTGCACGGCGGCGCTGGG
CGTCCTCCACGTCGGCGCCATCGTCTTCGCGTTCCAGGATCGCTACCCCGACATCTCGGTGGATCTGAGCCTCACCGACG
AGCGCGTGGATCTCGTCCGCGAGGGCATGGATCTGGCGATCCGCCTGGGTCCCCTGGCCGACAGCGCGCTGAGACTGCGG
GCCGTCGGGCGGTCGGAGCGTCGGCTCGTCGCCGCCCCCGCCTACCTGGCGGCGCGCGGCCGGCCCCTGACGCCGGAGGA
TCTCGTCGGCCACGAGGGGATCCGCATGACGAACGTGGCCGGGAGCGGTACCCTGGTCCTCGAAGGACCGGAGGCCGGGC
GTCGCGCGGTGCCCTTCGGCGGCCGCCTCCGGGTCGATCACGGGCTGGCCGCGCGGGCGGCTGTCCTGGCCGGGCGGGGC
ATCGCGCCCGCGCACCGCTGGCTCGTCGCGGATTGCCTCGCCGACGGCCGGCTCGAGGCGGTCCTGCCGGACTACGCCCT
GCCGTCGGTGCCGCTCAGCCTGCTGATCGTGCCCGAGCGGGCCGCGATCGCGCGGGTGCGGCTGCTGGCGGATTTCCTCG
CCGCGGAGCTCCGCGCGGTCCCGGGGATCGTGCCGGAGCCCGCCGCGCGGGCGGCGTCGGCTCAGCGATAG

Upstream 100 bases:

>100_bases
GGTGTTCATGGTGCGGGCTCCGTCGTGGCGCGGGACCCCCTGCTAGCCCAGGGCCGATCGTTCCGGTACGTGAGGCGTCC
GGATAGGATTATGCCGCGGA

Downstream 100 bases:

>100_bases
CCGTAGCGACTCTTGGCGGCGGCGAGCAGGTGCAGGGCCTCGCCCGACTTGCCGGCCGAGCACTCGGCGGCGGCGTGGGC
GAGGTCGGCGTTGAAGCGGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MDRSDVTLERMRSFVRVAERGSLSAVAREFGVGQSTITRHLRELEDALGVALISRTTRRLALTAEGLAYYENSLRILHLV
EQAGAEARDRRHAPAGTVRLSCTAALGVLHVGAIVFAFQDRYPDISVDLSLTDERVDLVREGMDLAIRLGPLADSALRLR
AVGRSERRLVAAPAYLAARGRPLTPEDLVGHEGIRMTNVAGSGTLVLEGPEAGRRAVPFGGRLRVDHGLAARAAVLAGRG
IAPAHRWLVADCLADGRLEAVLPDYALPSVPLSLLIVPERAAIARVRLLADFLAAELRAVPGIVPEPAARAASAQR

Sequences:

>Translated_316_residues
MDRSDVTLERMRSFVRVAERGSLSAVAREFGVGQSTITRHLRELEDALGVALISRTTRRLALTAEGLAYYENSLRILHLV
EQAGAEARDRRHAPAGTVRLSCTAALGVLHVGAIVFAFQDRYPDISVDLSLTDERVDLVREGMDLAIRLGPLADSALRLR
AVGRSERRLVAAPAYLAARGRPLTPEDLVGHEGIRMTNVAGSGTLVLEGPEAGRRAVPFGGRLRVDHGLAARAAVLAGRG
IAPAHRWLVADCLADGRLEAVLPDYALPSVPLSLLIVPERAAIARVRLLADFLAAELRAVPGIVPEPAARAASAQR
>Mature_316_residues
MDRSDVTLERMRSFVRVAERGSLSAVAREFGVGQSTITRHLRELEDALGVALISRTTRRLALTAEGLAYYENSLRILHLV
EQAGAEARDRRHAPAGTVRLSCTAALGVLHVGAIVFAFQDRYPDISVDLSLTDERVDLVREGMDLAIRLGPLADSALRLR
AVGRSERRLVAAPAYLAARGRPLTPEDLVGHEGIRMTNVAGSGTLVLEGPEAGRRAVPFGGRLRVDHGLAARAAVLAGRG
IAPAHRWLVADCLADGRLEAVLPDYALPSVPLSLLIVPERAAIARVRLLADFLAAELRAVPGIVPEPAARAASAQR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=31.6498316498317, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=33.7979094076655, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789639, Length=259, Percent_Identity=32.4324324324324, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=31.9587628865979, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=26.6666666666667, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787128, Length=262, Percent_Identity=29.0076335877863, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=26.0714285714286, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693105, Length=279, Percent_Identity=24.0143369175627, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI157672245, Length=221, Percent_Identity=23.9819004524887, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788481, Length=266, Percent_Identity=26.6917293233083, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=184, Percent_Identity=27.1739130434783, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787601, Length=137, Percent_Identity=24.8175182481752, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33873; Mature: 33873

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSDVTLERMRSFVRVAERGSLSAVAREFGVGQSTITRHLRELEDALGVALISRTTRRL
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHE
ALTAEGLAYYENSLRILHLVEQAGAEARDRRHAPAGTVRLSCTAALGVLHVGAIVFAFQD
EEEHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
RYPDISVDLSLTDERVDLVREGMDLAIRLGPLADSALRLRAVGRSERRLVAAPAYLAARG
CCCCEEEEEEECHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCEEEECCHHHHHCC
RPLTPEDLVGHEGIRMTNVAGSGTLVLEGPEAGRRAVPFGGRLRVDHGLAARAAVLAGRG
CCCCHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHCCC
IAPAHRWLVADCLADGRLEAVLPDYALPSVPLSLLIVPERAAIARVRLLADFLAAELRAV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHC
PGIVPEPAARAASAQR
CCCCCCCHHHHCCCCC
>Mature Secondary Structure
MDRSDVTLERMRSFVRVAERGSLSAVAREFGVGQSTITRHLRELEDALGVALISRTTRRL
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHE
ALTAEGLAYYENSLRILHLVEQAGAEARDRRHAPAGTVRLSCTAALGVLHVGAIVFAFQD
EEEHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHC
RYPDISVDLSLTDERVDLVREGMDLAIRLGPLADSALRLRAVGRSERRLVAAPAYLAARG
CCCCEEEEEEECHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCEEEECCHHHHHCC
RPLTPEDLVGHEGIRMTNVAGSGTLVLEGPEAGRRAVPFGGRLRVDHGLAARAAVLAGRG
CCCCHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHCCC
IAPAHRWLVADCLADGRLEAVLPDYALPSVPLSLLIVPERAAIARVRLLADFLAAELRAV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHC
PGIVPEPAARAASAQR
CCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]