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The map label for this gene is yjbJ [H]

Identifier: 170747245

GI number: 170747245

Start: 855976

End: 856932

Strand: Reverse

Name: yjbJ [H]

Synonym: Mrad2831_0812

Alternate gene names: 170747245

Gene position: 856932-855976 (Counterclockwise)

Preceding gene: 170747247

Following gene: 170747244

Centisome position: 14.1

GC content: 74.82

Gene sequence:

>957_bases
ATGCGCGGCGTGACCGCCCCGCCCCTGCGCCTCGGCGCGCTGCTCCTCGCCCTCGCCTGCGCGCATCCGGCCGCGGCCGC
CCCGTCCGAGGGAGCGACCGAGACGGTCGAGCAGGCGCTGTGCCGCCTCATCGACGACTCCGCCAAGGCCCGCGGCCTGC
CGGTGCCGTTCCTGACACGCCTGATCTGGCGCGAGAGCAGCTTCCGCGTGGGCGTCGTCAGCCCGGCCGGGGCGCAGGGC
GTCGCGCAGTTCATGCCCGGGACGGCCCGCGAGCGCGGCCTCCTCGACCCGTTCGACCCCGAGCAGGCGATCCCGCACGC
GGCCCATCTGCTCGCCGACCTGAGGACCCAGTTCGGCAATCTGGGTCTCGCCGCCGCGGCCTATAACGGCGGCGCCGCGC
GGGTCAGCAAGTGGCTCGCCGGCGACGGTGGCCTGCCCGCCGAGACCCGGGCCTACGTGGCCGCCATCACGGGCCGGCCG
GTGGACGACTGGCGGACCGGCCCGGCCAAGGGGATCGAGTTTCCGGAAGGCGGCGCGCCCGCCAAGCCCGAGAGCAAGCC
CGAGGGCAAGGACGCGAAGGACCAGAAGGACGCCAAGGTGGAGGTGAAGCCGGATGCGCCGCAGACCTGCCTGCAGGTCA
CCGCCAGCCTGCGCGTCCCGTCGCGCGGCGACCGCTTCGCCCTCGGCCTCAACGAGGGGCCGGCCGCCCCCTGGGGCATC
CAGCTCGCCGGCAACTTCTCCAAGGCTCTGGCGCTGCAGAGCTTCAACCGCGCGCGGAGCGCCTACGCCGGGGTGATCGG
CGAGGTGCGGCCGATGATCATCGGCACGCGCCTGCGCAACCGCGGGACCCGCGCCTTCTACCGGATCCGCATCCCGGCGC
AGAGCCGGCAGGCGGCCGACGACCTGTGCGGCCGCATCCGTAAGGTCGGCGGTGCCTGCTTCGTGCTGCGGACCTGA

Upstream 100 bases:

>100_bases
GTCGCGCCAGTGGGCAAAGGTGCAACGCTCGGGCACGCGGGCCGGATGTTCCCCCAGGAAGGTTGGCCGCGCGGATTGAT
CCGCCCCGGCGCGTCGCTAC

Downstream 100 bases:

>100_bases
TTTTTCGTGATCCGCCCCGAGACCGTCATCAATCCGCCCCGAGGGCGGTGCGGTATGGCCCGGTCGGGACAACCGGATGG
GATATGACACGTCTCCTCAC

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRGVTAPPLRLGALLLALACAHPAAAAPSEGATETVEQALCRLIDDSAKARGLPVPFLTRLIWRESSFRVGVVSPAGAQG
VAQFMPGTARERGLLDPFDPEQAIPHAAHLLADLRTQFGNLGLAAAAYNGGAARVSKWLAGDGGLPAETRAYVAAITGRP
VDDWRTGPAKGIEFPEGGAPAKPESKPEGKDAKDQKDAKVEVKPDAPQTCLQVTASLRVPSRGDRFALGLNEGPAAPWGI
QLAGNFSKALALQSFNRARSAYAGVIGEVRPMIIGTRLRNRGTRAFYRIRIPAQSRQAADDLCGRIRKVGGACFVLRT

Sequences:

>Translated_318_residues
MRGVTAPPLRLGALLLALACAHPAAAAPSEGATETVEQALCRLIDDSAKARGLPVPFLTRLIWRESSFRVGVVSPAGAQG
VAQFMPGTARERGLLDPFDPEQAIPHAAHLLADLRTQFGNLGLAAAAYNGGAARVSKWLAGDGGLPAETRAYVAAITGRP
VDDWRTGPAKGIEFPEGGAPAKPESKPEGKDAKDQKDAKVEVKPDAPQTCLQVTASLRVPSRGDRFALGLNEGPAAPWGI
QLAGNFSKALALQSFNRARSAYAGVIGEVRPMIIGTRLRNRGTRAFYRIRIPAQSRQAADDLCGRIRKVGGACFVLRT
>Mature_318_residues
MRGVTAPPLRLGALLLALACAHPAAAAPSEGATETVEQALCRLIDDSAKARGLPVPFLTRLIWRESSFRVGVVSPAGAQG
VAQFMPGTARERGLLDPFDPEQAIPHAAHLLADLRTQFGNLGLAAAAYNGGAARVSKWLAGDGGLPAETRAYVAAITGRP
VDDWRTGPAKGIEFPEGGAPAKPESKPEGKDAKDQKDAKVEVKPDAPQTCLQVTASLRVPSRGDRFALGLNEGPAAPWGI
QLAGNFSKALALQSFNRARSAYAGVIGEVRPMIIGTRLRNRGTRAFYRIRIPAQSRQAADDLCGRIRKVGGACFVLRT

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 33555; Mature: 33555

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGVTAPPLRLGALLLALACAHPAAAAPSEGATETVEQALCRLIDDSAKARGLPVPFLTR
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LIWRESSFRVGVVSPAGAQGVAQFMPGTARERGLLDPFDPEQAIPHAAHLLADLRTQFGN
HHHCCCCCEEEEECCCCHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
LGLAAAAYNGGAARVSKWLAGDGGLPAETRAYVAAITGRPVDDWRTGPAKGIEFPEGGAP
CCEEEEECCCCHHHHHHHHCCCCCCCCHHHEEEEEECCCCCHHCCCCCCCCCCCCCCCCC
AKPESKPEGKDAKDQKDAKVEVKPDAPQTCLQVTASLRVPSRGDRFALGLNEGPAAPWGI
CCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCE
QLAGNFSKALALQSFNRARSAYAGVIGEVRPMIIGTRLRNRGTRAFYRIRIPAQSRQAAD
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
DLCGRIRKVGGACFVLRT
HHHHHHHHCCCEEEEEEC
>Mature Secondary Structure
MRGVTAPPLRLGALLLALACAHPAAAAPSEGATETVEQALCRLIDDSAKARGLPVPFLTR
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LIWRESSFRVGVVSPAGAQGVAQFMPGTARERGLLDPFDPEQAIPHAAHLLADLRTQFGN
HHHCCCCCEEEEECCCCHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
LGLAAAAYNGGAARVSKWLAGDGGLPAETRAYVAAITGRPVDDWRTGPAKGIEFPEGGAP
CCEEEEECCCCHHHHHHHHCCCCCCCCHHHEEEEEECCCCCHHCCCCCCCCCCCCCCCCC
AKPESKPEGKDAKDQKDAKVEVKPDAPQTCLQVTASLRVPSRGDRFALGLNEGPAAPWGI
CCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCE
QLAGNFSKALALQSFNRARSAYAGVIGEVRPMIIGTRLRNRGTRAFYRIRIPAQSRQAAD
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
DLCGRIRKVGGACFVLRT
HHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]