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The map label for this gene is ispE

Identifier: 170079011

GI number: 170079011

Start: 2503213

End: 2504163

Strand: Direct

Name: ispE

Synonym: SYNPCC7002_A2416

Alternate gene names: 170079011

Gene position: 2503213-2504163 (Clockwise)

Preceding gene: 170079006

Following gene: 170079012

Centisome position: 83.22

GC content: 54.47

Gene sequence:

>951_bases
ATGTCCGCCTATCTTCTGACTGCCCCCGCAAAAATTAATCTCTACCTCGAAATTTTGGGCGATCGCCCCGACGGGTTCCA
CGAGTTGGTGATGGTGATGCAGACCATTGGCTTATATGACCGGATTGAGGTGCGACAAAACCGTAGCGGCGACATCAATC
TCTATTGCCAGCATCCCGAAGTCCCCCTCACCGCTAGCAATATTGCCTACCGAGCCGCCCAGTTAATGATGACGCGCTTT
GCAGATATCTACGCCAGGGTGGGTGGTGGAGTTGACATTACCATCGAAAAAAATATCCCCGTGGCGGCGGGACTGGCTGG
GGGGTCAACGGATGGCGCAGCGGTATTGGTGGGGTTGAATTTGCTCTGGGATTTAGGGTTAACCCAACCGGAATTACAAA
CCTTAGCAGCAGAACTGGGTTCGGATGTGCCCTTTTGCATTGGTGGGGGGACGGTGATCGCCACCGGACGGGGAGAAATT
TTAGACCCATTGCCCGATCTCGATGGCATCCCGATTGTCCTCGCAAAGTACAGCAACATTGCTGTTTCTACCCCCTGGGC
CTACCAAACCTACCGCGCCCAATTTGGGGAGCATTATCTCCAAGATGCGGCGAGTTGGCGTACCCGCATGACACAAATCC
ATGCTGGCGGCCTGATCAAAGCCATTCAGCAAAAAAATATCCAGGCGATCGCCCATGAACTCCATAACGATCTCGAAAAA
GTCGTTTTACCCGAATTTCCCCAGGTGCAACAACTGCGTGACTGCTTTACCAAACACGGGGCGATCGGCACGATGATGTC
GGGCTCCGGCCCCAGTGTGTTTGCCCTCTGCGAGACCCTCGCCCAAGCCCAAGCCCTCAAAGTCCAAGTTGCCCAAGAAT
TAAACGACCCAGCCCTCGATCTTTGGGTGACGCACATTGCCAGCCCCGGCATCCAAGCGGACTTCCGTTAA

Upstream 100 bases:

>100_bases
CAAGAAATATTAAGAGTCAAGCTTGGCTCATATTGGGAAGGATTTCCAAAAAACAAGGGAAACTAGATAATGGTCTCGAT
TTACTGCTTGGTACTGTGTG

Downstream 100 bases:

>100_bases
CTTTCCATGACTTAAAATAGTCACTTTTGAGTAAGTCCCTAAAATAACTAAGAGGCAATAAAGCAAAAAGCAATCTACCT
GAAAGCAGTTAGATTGCCCC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSAYLLTAPAKINLYLEILGDRPDGFHELVMVMQTIGLYDRIEVRQNRSGDINLYCQHPEVPLTASNIAYRAAQLMMTRF
ADIYARVGGGVDITIEKNIPVAAGLAGGSTDGAAVLVGLNLLWDLGLTQPELQTLAAELGSDVPFCIGGGTVIATGRGEI
LDPLPDLDGIPIVLAKYSNIAVSTPWAYQTYRAQFGEHYLQDAASWRTRMTQIHAGGLIKAIQQKNIQAIAHELHNDLEK
VVLPEFPQVQQLRDCFTKHGAIGTMMSGSGPSVFALCETLAQAQALKVQVAQELNDPALDLWVTHIASPGIQADFR

Sequences:

>Translated_316_residues
MSAYLLTAPAKINLYLEILGDRPDGFHELVMVMQTIGLYDRIEVRQNRSGDINLYCQHPEVPLTASNIAYRAAQLMMTRF
ADIYARVGGGVDITIEKNIPVAAGLAGGSTDGAAVLVGLNLLWDLGLTQPELQTLAAELGSDVPFCIGGGTVIATGRGEI
LDPLPDLDGIPIVLAKYSNIAVSTPWAYQTYRAQFGEHYLQDAASWRTRMTQIHAGGLIKAIQQKNIQAIAHELHNDLEK
VVLPEFPQVQQLRDCFTKHGAIGTMMSGSGPSVFALCETLAQAQALKVQVAQELNDPALDLWVTHIASPGIQADFR
>Mature_315_residues
SAYLLTAPAKINLYLEILGDRPDGFHELVMVMQTIGLYDRIEVRQNRSGDINLYCQHPEVPLTASNIAYRAAQLMMTRFA
DIYARVGGGVDITIEKNIPVAAGLAGGSTDGAAVLVGLNLLWDLGLTQPELQTLAAELGSDVPFCIGGGTVIATGRGEIL
DPLPDLDGIPIVLAKYSNIAVSTPWAYQTYRAQFGEHYLQDAASWRTRMTQIHAGGLIKAIQQKNIQAIAHELHNDLEKV
VLPEFPQVQQLRDCFTKHGAIGTMMSGSGPSVFALCETLAQAQALKVQVAQELNDPALDLWVTHIASPGIQADFR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=290, Percent_Identity=29.6551724137931, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_SYNP2 (B1XK39)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001735649.1
- ProteinModelPortal:   B1XK39
- SMR:   B1XK39
- GeneID:   6056602
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A2416
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK02534
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 34246; Mature: 34115

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYLLTAPAKINLYLEILGDRPDGFHELVMVMQTIGLYDRIEVRQNRSGDINLYCQHPE
CCCEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCC
VPLTASNIAYRAAQLMMTRFADIYARVGGGVDITIEKNIPVAAGLAGGSTDGAAVLVGLN
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCCCEEEHHHH
LLWDLGLTQPELQTLAAELGSDVPFCIGGGTVIATGRGEILDPLPDLDGIPIVLAKYSNI
HHHHCCCCCHHHHHHHHHHCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCEEEEECCCE
AVSTPWAYQTYRAQFGEHYLQDAASWRTRMTQIHAGGLIKAIQQKNIQAIAHELHNDLEK
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VVLPEFPQVQQLRDCFTKHGAIGTMMSGSGPSVFALCETLAQAQALKVQVAQELNDPALD
HHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LWVTHIASPGIQADFR
HHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAYLLTAPAKINLYLEILGDRPDGFHELVMVMQTIGLYDRIEVRQNRSGDINLYCQHPE
CCEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEECCC
VPLTASNIAYRAAQLMMTRFADIYARVGGGVDITIEKNIPVAAGLAGGSTDGAAVLVGLN
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCCCEEEHHHH
LLWDLGLTQPELQTLAAELGSDVPFCIGGGTVIATGRGEILDPLPDLDGIPIVLAKYSNI
HHHHCCCCCHHHHHHHHHHCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCEEEEECCCE
AVSTPWAYQTYRAQFGEHYLQDAASWRTRMTQIHAGGLIKAIQQKNIQAIAHELHNDLEK
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
VVLPEFPQVQQLRDCFTKHGAIGTMMSGSGPSVFALCETLAQAQALKVQVAQELNDPALD
HHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LWVTHIASPGIQADFR
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA