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The map label for this gene is ytcB [H]

Identifier: 170078425

GI number: 170078425

Start: 1906348

End: 1907295

Strand: Direct

Name: ytcB [H]

Synonym: SYNPCC7002_A1819

Alternate gene names: 170078425

Gene position: 1906348-1907295 (Clockwise)

Preceding gene: 170078421

Following gene: 170078426

Centisome position: 63.37

GC content: 51.16

Gene sequence:

>948_bases
ATGTCGAAGGTCATTGTCACAGGCGCTGCGGGATTCATTGGCTCTAGCCTCGCAGAAACTCTCCTCCAGCAGGGGATTAC
AGTGGTGGGTGTTGATCAGGTGAATGACTATTACGACATAACTCTCAAGCGTAAAAATCTAGCCGCCCTCGCCCAGTACC
AAAATTTCCAACTCATCGAAGCAGATATACAGCACCTCGATTGGGAAGATCTGCTGAAAGGTGTGAGTGTGGTTTACCAT
CAGGCAGCCCAAGCCGGGGTACGGGCCAGTTGGGGCATCGGCTTTCGGGACTATACCGAACGAAATATTAATTCCACCCA
GGTAATGCTCGAAGCGGCGAAAAAGGTCGGTACTTTAGAACGTTTTGTCTATGCGGGTACATCCTCGGTTTATGGCGATG
CGGAAACCATGCCCACCCCAGAAACCATTTGTCCCCAGCCCGTTTCTCCCTATGGCATTACAAAATTAGCGGCGGAACGG
CTCTGCTGGCTTTATTTAAAAAATTACGCTGTCCCGGTCACATCCCTGCGTTACTTCACGGTGTATGGCCCCCGACAACG
GCCCGATATGGCTTTCCATAAGTTTTTCCGGGCGGCAATCCTGGGTGAAACCATTGGCATTTACGGCGATGGAAAACAAA
CCCGTGATTTTACCTATATCAGCGACGCAGTACAGGCGAACCTCTTAGCCGCCCAGGAACCAAAGGCGATTGGCGAAATT
TTCAATATCGGCGGTGGCAGTCGGGTGATTTTAAATGATGTGCTCGATGAAATTGACCAGATCGTTGGCAAACCGATTCC
ACGAAACTATGGCGATCGCGCCCGGGGAGATGCCAGACATACCAGCGCCGATGTGACCAAGGCGAAAACGATTTTGGGTT
ATGACCCCCAGGTGAAACTCAGTGAGGGCCTGCAGCGAGAATGGGACTGGATTCAAAGTCTGTATTAG

Upstream 100 bases:

>100_bases
CAAAATTGGGGGAGTGCTAATGGCTGAAAAAAAACTTTGCGATCGCCTAACCCCATCCTCTATTTTGGAAACATCAAATT
TTTCTCCCCCGAACATCATC

Downstream 100 bases:

>100_bases
TTTGTATGAAAACAATCCCTGAGGCGAATTGATTATGGCGCAAAATTTACCCGCTCCCCCCCAACCTTTGCCCGATAAAC
TTTGGGGTGAAAATTGGCGT

Product: NAD-dependent epimerase/dehydratase family protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSKVIVTGAAGFIGSSLAETLLQQGITVVGVDQVNDYYDITLKRKNLAALAQYQNFQLIEADIQHLDWEDLLKGVSVVYH
QAAQAGVRASWGIGFRDYTERNINSTQVMLEAAKKVGTLERFVYAGTSSVYGDAETMPTPETICPQPVSPYGITKLAAER
LCWLYLKNYAVPVTSLRYFTVYGPRQRPDMAFHKFFRAAILGETIGIYGDGKQTRDFTYISDAVQANLLAAQEPKAIGEI
FNIGGGSRVILNDVLDEIDQIVGKPIPRNYGDRARGDARHTSADVTKAKTILGYDPQVKLSEGLQREWDWIQSLY

Sequences:

>Translated_315_residues
MSKVIVTGAAGFIGSSLAETLLQQGITVVGVDQVNDYYDITLKRKNLAALAQYQNFQLIEADIQHLDWEDLLKGVSVVYH
QAAQAGVRASWGIGFRDYTERNINSTQVMLEAAKKVGTLERFVYAGTSSVYGDAETMPTPETICPQPVSPYGITKLAAER
LCWLYLKNYAVPVTSLRYFTVYGPRQRPDMAFHKFFRAAILGETIGIYGDGKQTRDFTYISDAVQANLLAAQEPKAIGEI
FNIGGGSRVILNDVLDEIDQIVGKPIPRNYGDRARGDARHTSADVTKAKTILGYDPQVKLSEGLQREWDWIQSLY
>Mature_314_residues
SKVIVTGAAGFIGSSLAETLLQQGITVVGVDQVNDYYDITLKRKNLAALAQYQNFQLIEADIQHLDWEDLLKGVSVVYHQ
AAQAGVRASWGIGFRDYTERNINSTQVMLEAAKKVGTLERFVYAGTSSVYGDAETMPTPETICPQPVSPYGITKLAAERL
CWLYLKNYAVPVTSLRYFTVYGPRQRPDMAFHKFFRAAILGETIGIYGDGKQTRDFTYISDAVQANLLAAQEPKAIGEIF
NIGGGSRVILNDVLDEIDQIVGKPIPRNYGDRARGDARHTSADVTKAKTILGYDPQVKLSEGLQREWDWIQSLY

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=323, Percent_Identity=30.6501547987616, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI42516563, Length=311, Percent_Identity=31.8327974276527, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=28.3185840707965, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788353, Length=347, Percent_Identity=29.6829971181556, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786974, Length=339, Percent_Identity=26.5486725663717, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=324, Percent_Identity=27.4691358024691, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=28.4023668639053, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=343, Percent_Identity=26.530612244898, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=341, Percent_Identity=26.9794721407625, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=323, Percent_Identity=25.3869969040248, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319493, Length=343, Percent_Identity=27.4052478134111, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356223, Length=320, Percent_Identity=30.3125, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19923002, Length=338, Percent_Identity=27.5147928994083, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34957; Mature: 34826

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVIVTGAAGFIGSSLAETLLQQGITVVGVDQVNDYYDITLKRKNLAALAQYQNFQLIE
CCCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCHHHHHHHHCCCEEEH
ADIQHLDWEDLLKGVSVVYHQAAQAGVRASWGIGFRDYTERNINSTQVMLEAAKKVGTLE
HHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
RFVYAGTSSVYGDAETMPTPETICPQPVSPYGITKLAAERLCWLYLKNYAVPVTSLRYFT
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
VYGPRQRPDMAFHKFFRAAILGETIGIYGDGKQTRDFTYISDAVQANLLAAQEPKAIGEI
EECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
FNIGGGSRVILNDVLDEIDQIVGKPIPRNYGDRARGDARHTSADVTKAKTILGYDPQVKL
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
SEGLQREWDWIQSLY
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVIVTGAAGFIGSSLAETLLQQGITVVGVDQVNDYYDITLKRKNLAALAQYQNFQLIE
CCEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEECCHHHHHHHHCCCEEEH
ADIQHLDWEDLLKGVSVVYHQAAQAGVRASWGIGFRDYTERNINSTQVMLEAAKKVGTLE
HHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
RFVYAGTSSVYGDAETMPTPETICPQPVSPYGITKLAAERLCWLYLKNYAVPVTSLRYFT
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
VYGPRQRPDMAFHKFFRAAILGETIGIYGDGKQTRDFTYISDAVQANLLAAQEPKAIGEI
EECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
FNIGGGSRVILNDVLDEIDQIVGKPIPRNYGDRARGDARHTSADVTKAKTILGYDPQVKL
HHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
SEGLQREWDWIQSLY
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]