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The map label for this gene is hypB [H]

Identifier: 170078354

GI number: 170078354

Start: 1833065

End: 1833760

Strand: Direct

Name: hypB [H]

Synonym: SYNPCC7002_A1747

Alternate gene names: 170078354

Gene position: 1833065-1833760 (Clockwise)

Preceding gene: 170078353

Following gene: 170078355

Centisome position: 60.94

GC content: 46.98

Gene sequence:

>696_bases
ATGCACCAGACCTTTGACGCTGCCCTGGGGGTCAATCTCCTCCATGCCAACCAAGACCTTGCTGACCACAACCGTGAACA
TTTTGATGCCTGGGGCCTGACCTGCCTCAATGTGATGAGTAGTCCCGGTGCTGGTAAAACTGTTTTACTCGAAAAAACCC
TGGCCGCCCTAAGAGATGACTTAAAAATGGCCGTCATTGAAGGGGATATGACCACAGAACTCGATGCCGATCGCCTCCGT
CAATACGGCGTGCCTGTGATCCCCATTAATACGGGGCGTTCCTGCCATTTAGATGCCAAGATGGTTTCCGGGGGATTGCA
CCGCCTCGAACAGGAATATAACCCAAATGATTTTGATCTATTGCTTGTGGAAAATGTAGGAAATCTCGTTTGTCCCGCCG
AATTTGAAGTGGGTGAACATGCAAAAATTGCGCTCCTTAGTGTGACGGAAGGAGAAGATAAACCCCTCAAATATCCCGTT
ATGTTCCAAGAAGCGGACTGTTTATTGGTTACAAAAATTGACCTGGTTCCCTATCTAAACATTGATGTCCAACGGATTAT
TGATCATGTCCGCCAAATGAATTCCCACGCGACGATTATCCCCGTTTCTGCCCAGACTGGGGAAGGTTTAGAAACTTGGT
ATGACTGGGTTAAAAAAAGTTGTCATCGGGAAGATGTAATGAAAGTTACAGCATAA

Upstream 100 bases:

>100_bases
CTGTCATCAGCCTATGGATGATATCCGCTCCGGTCGTGAGCTAAAAATCGACCGCATTGAATACGCTCCCTTAACTCTCC
ATTCCTAGGAACTAACCACC

Downstream 100 bases:

>100_bases
TTTGTCCGAAAACAGGATAAACAACAGGCAAGGTGCGATCGCCTTTTAATTGATCCCTTGCGTTTATTCTTGTCATTTTC
CTATGCTAAAACTGCCTAGT

Product: hydrogenase accessory protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MHQTFDAALGVNLLHANQDLADHNREHFDAWGLTCLNVMSSPGAGKTVLLEKTLAALRDDLKMAVIEGDMTTELDADRLR
QYGVPVIPINTGRSCHLDAKMVSGGLHRLEQEYNPNDFDLLLVENVGNLVCPAEFEVGEHAKIALLSVTEGEDKPLKYPV
MFQEADCLLVTKIDLVPYLNIDVQRIIDHVRQMNSHATIIPVSAQTGEGLETWYDWVKKSCHREDVMKVTA

Sequences:

>Translated_231_residues
MHQTFDAALGVNLLHANQDLADHNREHFDAWGLTCLNVMSSPGAGKTVLLEKTLAALRDDLKMAVIEGDMTTELDADRLR
QYGVPVIPINTGRSCHLDAKMVSGGLHRLEQEYNPNDFDLLLVENVGNLVCPAEFEVGEHAKIALLSVTEGEDKPLKYPV
MFQEADCLLVTKIDLVPYLNIDVQRIIDHVRQMNSHATIIPVSAQTGEGLETWYDWVKKSCHREDVMKVTA
>Mature_231_residues
MHQTFDAALGVNLLHANQDLADHNREHFDAWGLTCLNVMSSPGAGKTVLLEKTLAALRDDLKMAVIEGDMTTELDADRLR
QYGVPVIPINTGRSCHLDAKMVSGGLHRLEQEYNPNDFDLLLVENVGNLVCPAEFEVGEHAKIALLSVTEGEDKPLKYPV
MFQEADCLLVTKIDLVPYLNIDVQRIIDHVRQMNSHATIIPVSAQTGEGLETWYDWVKKSCHREDVMKVTA

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=208, Percent_Identity=50, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 25759; Mature: 25759

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQTFDAALGVNLLHANQDLADHNREHFDAWGLTCLNVMSSPGAGKTVLLEKTLAALRDD
CCCCHHHHHCEEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHC
LKMAVIEGDMTTELDADRLRQYGVPVIPINTGRSCHLDAKMVSGGLHRLEQEYNPNDFDL
CEEEEEECCCCCCCCHHHHHHCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCEEE
LLVENVGNLVCPAEFEVGEHAKIALLSVTEGEDKPLKYPVMFQEADCLLVTKIDLVPYLN
EEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCEECCEEECCCCEEEEEEECCCEECC
IDVQRIIDHVRQMNSHATIIPVSAQTGEGLETWYDWVKKSCHREDVMKVTA
CCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHEECC
>Mature Secondary Structure
MHQTFDAALGVNLLHANQDLADHNREHFDAWGLTCLNVMSSPGAGKTVLLEKTLAALRDD
CCCCHHHHHCEEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHC
LKMAVIEGDMTTELDADRLRQYGVPVIPINTGRSCHLDAKMVSGGLHRLEQEYNPNDFDL
CEEEEEECCCCCCCCHHHHHHCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCEEE
LLVENVGNLVCPAEFEVGEHAKIALLSVTEGEDKPLKYPVMFQEADCLLVTKIDLVPYLN
EEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCEECCEEECCCCEEEEEEECCCEECC
IDVQRIIDHVRQMNSHATIIPVSAQTGEGLETWYDWVKKSCHREDVMKVTA
CCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]