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The map label for this gene is hisZ

Identifier: 170077245

GI number: 170077245

Start: 651313

End: 652527

Strand: Reverse

Name: hisZ

Synonym: SYNPCC7002_A0621

Alternate gene names: 170077245

Gene position: 652527-651313 (Counterclockwise)

Preceding gene: 170077246

Following gene: 170077244

Centisome position: 21.69

GC content: 52.02

Gene sequence:

>1215_bases
ATGATTCATCAACCCCCCGCAGGTGCCAGGGATTTATTACCCCTCGAAGTTGCTCAAAAGGCCTGGATTAATGACAATCT
CCAACGGGTTTTTCAACAGTGGGGCTACCAGCGGATCGTCACCTCTACTTTGGAATGGTTGGAGACGCTCACGGCTGGCG
GGGCGGTGGATCGCACAAAGGTCATTCAACTACAAACGGCAGAAAGTCAAGCTTTGGGTTTGCGTCCAGAGTTAACGGCC
TCGATCGCCAGGGCGGCTGTCACCAGAATGGCCGAAAATACCTTTCCCCAACGGCTTTGTTACCGGGCCAATGTGTTTCG
TCATCCCCCCCGGGGCAGCCACGGCAAACAAATGGAGTTTTACCAAGCGGGGGTAGAGTTACTCTTTGCGGCAGGAATTG
TGGCGGATGCAGAAATTTTATTGCTTCTAGCAGATTCCCTCGATGCCCTGGGTTTAGAAGATTGGCAGTTAATTTTAGGA
GAGGCGGCCCTGGGGCGATCGCTCTTGGATCCGTTTCCTGAACCAGTAAGGGAAACAGTTCGTCATTGTGTGGCCAATTT
AGACCGGGTTGGGTTGCAGGAATTGCCCCTGGATGAGGATCTCAAGGCCTATGCCCTGGACATTTTTGATCTGCGTGGCG
AGCCGGAAACCATATTGGCGCGGGTGAGTCAGTTTGATCTGGGGCCAGAGGCGCAGGAAATCGTCGCGAATCTTAAGGCT
CTCTTTGCGTTATTGGCTGGGAGTACCCAGAAGCAACTGCCAATTATCTTGGATTTAACCCTAATTCAGACCTTTGATTA
CTACACGGGCATTGTGTTTGAGGTGGTTAATTTTGCCAATCATCAGTCTTATATCTTGGGTCAAGGTGGCCGCTATGATC
AGCTGCTGGGTCTGTATCATCCCCAACGGGAAAATCACCCCGGTATTGGCTTTTGCTTGAATATTGAAGAACTACACACC
TGTCTGTTGACAAGTCCCCAATTGCCCAAACAACTCGCTGGTAGTGCTTGGCTGGTGATTGCAACGGAACCCAATGCCCA
GCAGCAGGTATTTCACTATGCCCAAACCCTGCGTCAAGGGGATGAAATGGTGCGCGTCGAAGTGGAACTGGGAGGGCGAT
CGCCTGCCGAAATTTATGCCTATGCCCGCAGCAGCCACATTACCCACCTCGCCTGGATTGACCCCAGTGGCGAACCCAAA
CTCGAAACCCTCTAA

Upstream 100 bases:

>100_bases
AAGAAAGGGAGCGCCGATAAACAAGGGGGTTTATTTGGCGGTTTGTTTGGCGGGAAAAAGAAGTAGAATAGGCGACGGCA
AAGCTCAACATACGGATTTC

Downstream 100 bases:

>100_bases
CTTAAATTCTGTTTCCTTAAGATTTTTGAATCACTGCTAACGAAAAAATGCTTACCATTGCCATTCCCAAAGGTGGCCTC
CTCCCAGAGGCGATCGCCCT

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MIHQPPAGARDLLPLEVAQKAWINDNLQRVFQQWGYQRIVTSTLEWLETLTAGGAVDRTKVIQLQTAESQALGLRPELTA
SIARAAVTRMAENTFPQRLCYRANVFRHPPRGSHGKQMEFYQAGVELLFAAGIVADAEILLLLADSLDALGLEDWQLILG
EAALGRSLLDPFPEPVRETVRHCVANLDRVGLQELPLDEDLKAYALDIFDLRGEPETILARVSQFDLGPEAQEIVANLKA
LFALLAGSTQKQLPIILDLTLIQTFDYYTGIVFEVVNFANHQSYILGQGGRYDQLLGLYHPQRENHPGIGFCLNIEELHT
CLLTSPQLPKQLAGSAWLVIATEPNAQQQVFHYAQTLRQGDEMVRVEVELGGRSPAEIYAYARSSHITHLAWIDPSGEPK
LETL

Sequences:

>Translated_404_residues
MIHQPPAGARDLLPLEVAQKAWINDNLQRVFQQWGYQRIVTSTLEWLETLTAGGAVDRTKVIQLQTAESQALGLRPELTA
SIARAAVTRMAENTFPQRLCYRANVFRHPPRGSHGKQMEFYQAGVELLFAAGIVADAEILLLLADSLDALGLEDWQLILG
EAALGRSLLDPFPEPVRETVRHCVANLDRVGLQELPLDEDLKAYALDIFDLRGEPETILARVSQFDLGPEAQEIVANLKA
LFALLAGSTQKQLPIILDLTLIQTFDYYTGIVFEVVNFANHQSYILGQGGRYDQLLGLYHPQRENHPGIGFCLNIEELHT
CLLTSPQLPKQLAGSAWLVIATEPNAQQQVFHYAQTLRQGDEMVRVEVELGGRSPAEIYAYARSSHITHLAWIDPSGEPK
LETL
>Mature_404_residues
MIHQPPAGARDLLPLEVAQKAWINDNLQRVFQQWGYQRIVTSTLEWLETLTAGGAVDRTKVIQLQTAESQALGLRPELTA
SIARAAVTRMAENTFPQRLCYRANVFRHPPRGSHGKQMEFYQAGVELLFAAGIVADAEILLLLADSLDALGLEDWQLILG
EAALGRSLLDPFPEPVRETVRHCVANLDRVGLQELPLDEDLKAYALDIFDLRGEPETILARVSQFDLGPEAQEIVANLKA
LFALLAGSTQKQLPIILDLTLIQTFDYYTGIVFEVVNFANHQSYILGQGGRYDQLLGLYHPQRENHPGIGFCLNIEELHT
CLLTSPQLPKQLAGSAWLVIATEPNAQQQVFHYAQTLRQGDEMVRVEVELGGRSPAEIYAYARSSHITHLAWIDPSGEPK
LETL

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=352, Percent_Identity=26.4204545454545, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI15029520, Length=346, Percent_Identity=25.7225433526012, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24643061, Length=364, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_SYNP2 (B1XPZ9)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001733883.1
- ProteinModelPortal:   B1XPZ9
- SMR:   B1XPZ9
- GeneID:   6057357
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A0621
- HOGENOM:   HBG616575
- OMA:   YREHVKS
- ProtClustDB:   PRK12292
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 44972; Mature: 44972

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHQPPAGARDLLPLEVAQKAWINDNLQRVFQQWGYQRIVTSTLEWLETLTAGGAVDRTK
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEE
VIQLQTAESQALGLRPELTASIARAAVTRMAENTFPQRLCYRANVFRHPPRGSHGKQMEF
EEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
YQAGVELLFAAGIVADAEILLLLADSLDALGLEDWQLILGEAALGRSLLDPFPEPVRETV
HHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
RHCVANLDRVGLQELPLDEDLKAYALDIFDLRGEPETILARVSQFDLGPEAQEIVANLKA
HHHHHHHHHCCHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
LFALLAGSTQKQLPIILDLTLIQTFDYYTGIVFEVVNFANHQSYILGQGGRYDQLLGLYH
HHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCC
PQRENHPGIGFCLNIEELHTCLLTSPQLPKQLAGSAWLVIATEPNAQQQVFHYAQTLRQG
CCCCCCCCCEEEEEHHHHHHHHHCCCCCHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCC
DEMVRVEVELGGRSPAEIYAYARSSHITHLAWIDPSGEPKLETL
CCEEEEEEEECCCCHHHHHEEHHCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MIHQPPAGARDLLPLEVAQKAWINDNLQRVFQQWGYQRIVTSTLEWLETLTAGGAVDRTK
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEE
VIQLQTAESQALGLRPELTASIARAAVTRMAENTFPQRLCYRANVFRHPPRGSHGKQMEF
EEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
YQAGVELLFAAGIVADAEILLLLADSLDALGLEDWQLILGEAALGRSLLDPFPEPVRETV
HHHHHHHHHHHHHHHHHHEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
RHCVANLDRVGLQELPLDEDLKAYALDIFDLRGEPETILARVSQFDLGPEAQEIVANLKA
HHHHHHHHHCCHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
LFALLAGSTQKQLPIILDLTLIQTFDYYTGIVFEVVNFANHQSYILGQGGRYDQLLGLYH
HHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCC
PQRENHPGIGFCLNIEELHTCLLTSPQLPKQLAGSAWLVIATEPNAQQQVFHYAQTLRQG
CCCCCCCCCEEEEEHHHHHHHHHCCCCCHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCC
DEMVRVEVELGGRSPAEIYAYARSSHITHLAWIDPSGEPKLETL
CCEEEEEEEECCCCHHHHHEEHHCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA