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The map label for this gene is proA [C]

Identifier: 170077208

GI number: 170077208

Start: 609083

End: 610357

Strand: Direct

Name: proA [C]

Synonym: SYNPCC7002_A0583

Alternate gene names: 170077208

Gene position: 609083-610357 (Clockwise)

Preceding gene: 170077206

Following gene: 170077209

Centisome position: 20.25

GC content: 51.37

Gene sequence:

>1275_bases
ATGGCATCTACTCCCCCGTTAGAGAGCAGTGATCCGATTACGGCAGCCTTGCAGCGGGCCCATCAGGCTTCCCTAGAACT
AGAGTTACTCAAAGGTAGTGAACGTCATCTGGGTATTGAGGCGATCGCCACCGCCCTAGAGGCCAATATTTCCGTCATCC
TCGAAGCGAATACCAAGGATTTAGAGGCCAGCCGCGAAATGGCCGTGCCGAAGCTGATTCTGGATTGGTTAAAGTTGACC
CCCGAACGGCTGCAAAAAACAGTGCGTCTGCTGCGTCAACTGCCAAAACTACCCGATCCGCTGCAACGGGTGAAAAATGC
GACCTATCAAATTAGCGGTTACCAGACCTATTCCCAACGGATGCCCCTGGGGGTGATTTGTCTGATCCATGAAGCCTTGC
CCGAATTAGGGGCGATCGCCGCTGGACTTTGTTTAAAGACAGCCAATAGCCTCGTGATTCGCGGTTGCGGTGATGCCAGC
CATACCAATCATCTGATCGCTGAGATTATGCAAACGGCCCTTGCTGACCAGGGTTTACCCGCAGGCTGTTTAGAATCTCT
CTCTTCTGAGGAAGGTTGTACCGTACAACAGCTAGTGGCCCAGGATCGCTACTTGAACCTGATCATTCCCTATGGCCGTC
CCAACATGATTCAACAGGTGGTGCAACATGCCACCACCCCTGTCCTGAGAACCACCGTAGGCAATTGCTACCTCTACCTC
GCCCCCAGCGGTGATCTGGAATTGGCCAGTTGGGTGATCCAAGACAGCCATAATTACGAACCCGATGCCGTCAATGCCAT
CGAAAAAGTCCTGGTGTGTCCTGAGCAAAAAACATCCCATTTAATTAGTCTGTTTAAATCCCTCCAAGAACAAGACTTTG
AGCTACGGGGAGATGCGTCCCTCGTCGAAGAATTTCCTGAACACCTCGCCCCCATCGGTACCGAAGAAGAATGGCAGCAG
GCTTATTTGGAAAAAATTGTGGCGTTTAAACGGGTGCCGGATCTGGCCGCAGCCATCACTGAAATTAATTGCAACTCCGG
TGGCCATGCTGATTGTATTGTCACGGAGTCCTACCATGAAAGTCGGCAATTTTCCATGGGACTCGATAGTGCCTTGATCT
ATATCAATGCCTCCCCTTGTTTTTCGCGTCATCCCTTGGGGAGCGATTCGGTATTTCTTGGGGTCTCGAACCAAAAGGGA
AATCGCCGGGGTTTAATTGGCCTCGAAAGTTTTACGACCATTAAACAGGTGGTTCAGGGGGATAGTACCCTCTAG

Upstream 100 bases:

>100_bases
CTAGCTGGCAGGTTCTTGCGATCCCCTAGGGCAATCAGTATGATCTGGTGAGGCTTTACGCAAACTTTCGCGAGTACATT
GGGACTTCATGGGTAATCAA

Downstream 100 bases:

>100_bases
GTTTCAACGCCTCAAAGAACGCTGTCTCGATGGAGATGGCCTTTGTAAGCTTAAAAAGTTAAGGACAAGATTTATGTTTA
AAGCGATCGCCCTCAGTTCT

Product: aldehyde dehydrogenase (NAD) family protein

Products: L-glutamyl 5-phosphate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MASTPPLESSDPITAALQRAHQASLELELLKGSERHLGIEAIATALEANISVILEANTKDLEASREMAVPKLILDWLKLT
PERLQKTVRLLRQLPKLPDPLQRVKNATYQISGYQTYSQRMPLGVICLIHEALPELGAIAAGLCLKTANSLVIRGCGDAS
HTNHLIAEIMQTALADQGLPAGCLESLSSEEGCTVQQLVAQDRYLNLIIPYGRPNMIQQVVQHATTPVLRTTVGNCYLYL
APSGDLELASWVIQDSHNYEPDAVNAIEKVLVCPEQKTSHLISLFKSLQEQDFELRGDASLVEEFPEHLAPIGTEEEWQQ
AYLEKIVAFKRVPDLAAAITEINCNSGGHADCIVTESYHESRQFSMGLDSALIYINASPCFSRHPLGSDSVFLGVSNQKG
NRRGLIGLESFTTIKQVVQGDSTL

Sequences:

>Translated_424_residues
MASTPPLESSDPITAALQRAHQASLELELLKGSERHLGIEAIATALEANISVILEANTKDLEASREMAVPKLILDWLKLT
PERLQKTVRLLRQLPKLPDPLQRVKNATYQISGYQTYSQRMPLGVICLIHEALPELGAIAAGLCLKTANSLVIRGCGDAS
HTNHLIAEIMQTALADQGLPAGCLESLSSEEGCTVQQLVAQDRYLNLIIPYGRPNMIQQVVQHATTPVLRTTVGNCYLYL
APSGDLELASWVIQDSHNYEPDAVNAIEKVLVCPEQKTSHLISLFKSLQEQDFELRGDASLVEEFPEHLAPIGTEEEWQQ
AYLEKIVAFKRVPDLAAAITEINCNSGGHADCIVTESYHESRQFSMGLDSALIYINASPCFSRHPLGSDSVFLGVSNQKG
NRRGLIGLESFTTIKQVVQGDSTL
>Mature_423_residues
ASTPPLESSDPITAALQRAHQASLELELLKGSERHLGIEAIATALEANISVILEANTKDLEASREMAVPKLILDWLKLTP
ERLQKTVRLLRQLPKLPDPLQRVKNATYQISGYQTYSQRMPLGVICLIHEALPELGAIAAGLCLKTANSLVIRGCGDASH
TNHLIAEIMQTALADQGLPAGCLESLSSEEGCTVQQLVAQDRYLNLIIPYGRPNMIQQVVQHATTPVLRTTVGNCYLYLA
PSGDLELASWVIQDSHNYEPDAVNAIEKVLVCPEQKTSHLISLFKSLQEQDFELRGDASLVEEFPEHLAPIGTEEEWQQA
YLEKIVAFKRVPDLAAAITEINCNSGGHADCIVTESYHESRQFSMGLDSALIYINASPCFSRHPLGSDSVFLGVSNQKGN
RRGLIGLESFTTIKQVVQGDSTL

Specific function: Catalyzes The Nadph Dependent Reduction Of L-Gamma- Glutamyl 5-Phosphate Into L-Glutamate 5-Semialdehyde And Phosphate. The Product Spontaneously Undergoes Cyclization To Form 1-Pyrroline-5-Carboxylate. [C]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=378, Percent_Identity=27.5132275132275, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI21361368, Length=378, Percent_Identity=27.5132275132275, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1786438, Length=411, Percent_Identity=32.360097323601, Blast_Score=205, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17569967, Length=426, Percent_Identity=25.3521126760563, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17569969, Length=426, Percent_Identity=25.3521126760563, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324899, Length=427, Percent_Identity=26.6978922716628, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21357643, Length=415, Percent_Identity=26.5060240963855, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: 1.2.1.41

Molecular weight: Translated: 46427; Mature: 46295

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTPPLESSDPITAALQRAHQASLELELLKGSERHLGIEAIATALEANISVILEANTKD
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCH
LEASREMAVPKLILDWLKLTPERLQKTVRLLRQLPKLPDPLQRVKNATYQISGYQTYSQR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCHHHHC
MPLGVICLIHEALPELGAIAAGLCLKTANSLVIRGCGDASHTNHLIAEIMQTALADQGLP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCC
AGCLESLSSEEGCTVQQLVAQDRYLNLIIPYGRPNMIQQVVQHATTPVLRTTVGNCYLYL
HHHHHHCCCCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCHHHHEECCCEEEEE
APSGDLELASWVIQDSHNYEPDAVNAIEKVLVCPEQKTSHLISLFKSLQEQDFELRGDAS
ECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHH
LVEEFPEHLAPIGTEEEWQQAYLEKIVAFKRVPDLAAAITEINCNSGGHADCIVTESYHE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCEEEEEECCHHH
SRQFSMGLDSALIYINASPCFSRHPLGSDSVFLGVSNQKGNRRGLIGLESFTTIKQVVQG
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHCC
DSTL
CCCC
>Mature Secondary Structure 
ASTPPLESSDPITAALQRAHQASLELELLKGSERHLGIEAIATALEANISVILEANTKD
CCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCH
LEASREMAVPKLILDWLKLTPERLQKTVRLLRQLPKLPDPLQRVKNATYQISGYQTYSQR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCHHHHC
MPLGVICLIHEALPELGAIAAGLCLKTANSLVIRGCGDASHTNHLIAEIMQTALADQGLP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCC
AGCLESLSSEEGCTVQQLVAQDRYLNLIIPYGRPNMIQQVVQHATTPVLRTTVGNCYLYL
HHHHHHCCCCCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCHHHHEECCCEEEEE
APSGDLELASWVIQDSHNYEPDAVNAIEKVLVCPEQKTSHLISLFKSLQEQDFELRGDAS
ECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHH
LVEEFPEHLAPIGTEEEWQQAYLEKIVAFKRVPDLAAAITEINCNSGGHADCIVTESYHE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCCCEEEEEECCHHH
SRQFSMGLDSALIYINASPCFSRHPLGSDSVFLGVSNQKGNRRGLIGLESFTTIKQVVQG
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHCC
DSTL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 11 {phosphate}} 2.5 {DL-pyrroline-5-carboxylic} 0.18 {NADP+}} 0.05 {NADP+}} 2.2 {glutamic} [C]

Substrates: L-glutamate 5-semialdehyde; phosphate; NADP+

Specific reaction: L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: 3-(Phosphonoacetyl amido)-L-alanine; Ba2+; Co2+; Cu2+; Iodoacetamide; Iodoacetate; NADPH; Ni2+; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]