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The map label for this gene is prfB [H]

Identifier: 170076819

GI number: 170076819

Start: 196729

End: 197808

Strand: Direct

Name: prfB [H]

Synonym: SYNPCC7002_A0188

Alternate gene names: 170076819

Gene position: 196729-197808 (Clockwise)

Preceding gene: 170076813

Following gene: 170076820

Centisome position: 6.54

GC content: 52.87

Gene sequence:

>1080_bases
CTGTCCAAGCGCACCTGGGGAAAACCCAGGACTATCTTTGACCTAAAAACCTTAGATGCGAAGATTTTTGACCTCGAACA
ACAGGCGGCCCAGCCAGAATTTTGGGAGGATCAACAAGGGGCCCAGGACATTCTCCAGGAGCTAACGGAGCTAAAGGGCA
CCGTGGCTCAGTATCAACAGTGGCAGGGTCAGTTTGAAGATGCGGCGGCGATCGCCGAATTATTGACCGAAGCAGAAGAC
CCGGCTCTCCTCACGGAAGCGATGGAAAACCTTGATGATCTCCAGAAGGAACTAAACCGCTGGGAACTGCAACAGATGCT
CTCCGGGGAATATGACAACCTCGGTGCGGTGCTGACGATTAATGCCGGGGCTGGGGGCACCGATGCCCAGGACTGGGCGG
AGATGCTGTTGCGAATGTACACCCGCTGGAGTGAAAAACAAGGCTATAAGGTACGGCTGGCAGAAATTTCCGAAGGGGAC
GAAGCGGGCTTAAAATCGGCCACCCTCGAAGTGACCGGGCGTTACGCCTTTGGTTATCTCAAGCATGAAAAAGGCACCCA
TCGTTTGGTGCGAATCTCGCCCTTTAATGCCAATGGCAAACGCCAAACCAGTTTTGCTGGGGTGGAAGTGATGCCCATTT
TGGGAGACAATGCCGTTAAAGTCGATATTCCCGAAAAAGATTTAGAGATCACCACCTCCCGTGCAGGCGGTAAGGGGGGA
CAAAACGTCAATAAAGTCGAAACGGCGGTACGGATTGTCCATTTACCTACGGGGGTGGCTGTGCGCTGTACCCAAGAGCG
ATCGCAGTTACAAAACAAAGAAAAAGCCCTGGCGATCCTCAAGGCAAAATTATTAATCATTGCCCAGGAACAACGGGCCC
AGGAAATCGCTGAAATTCGGGGAGATATGGTGGAGGCCGCCTGGGGAAATCAGATCCGGAACTATGTTTTTCACCCTTAT
CAAATGGTCAAGGATCTGCGCACCAACCACGAAACGACCGATGTGGATGGGGTCATGGATGGCGACCTCGATGCTTTTAT
TGAAGCTTCCCTGCGCCAGGACACCTGGGTGGGAGACTAG

Upstream 100 bases:

>100_bases
ATCTGCCGATATTGCCGGCTGTCTCTGTACTTTTTGCGTATTTGCCACAACTTCCAAAGATCTATGGTGGAATTAACCGA
TTTAAAGCGTTTCCTCGAAA

Downstream 100 bases:

>100_bases
CACCGGGGAATGGGGAACGGTGAGCTAAAATGCAAGGTGGCAAAATTTCGTGGCTTCTTCAGTTTTGGATATTTTTCGCC
TCAAACGCTTCGATCAAGTC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSKRTWGKPRTIFDLKTLDAKIFDLEQQAAQPEFWEDQQGAQDILQELTELKGTVAQYQQWQGQFEDAAAIAELLTEAED
PALLTEAMENLDDLQKELNRWELQQMLSGEYDNLGAVLTINAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVRLAEISEGD
EAGLKSATLEVTGRYAFGYLKHEKGTHRLVRISPFNANGKRQTSFAGVEVMPILGDNAVKVDIPEKDLEITTSRAGGKGG
QNVNKVETAVRIVHLPTGVAVRCTQERSQLQNKEKALAILKAKLLIIAQEQRAQEIAEIRGDMVEAAWGNQIRNYVFHPY
QMVKDLRTNHETTDVDGVMDGDLDAFIEASLRQDTWVGD

Sequences:

>Translated_359_residues
MSKRTWGKPRTIFDLKTLDAKIFDLEQQAAQPEFWEDQQGAQDILQELTELKGTVAQYQQWQGQFEDAAAIAELLTEAED
PALLTEAMENLDDLQKELNRWELQQMLSGEYDNLGAVLTINAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVRLAEISEGD
EAGLKSATLEVTGRYAFGYLKHEKGTHRLVRISPFNANGKRQTSFAGVEVMPILGDNAVKVDIPEKDLEITTSRAGGKGG
QNVNKVETAVRIVHLPTGVAVRCTQERSQLQNKEKALAILKAKLLIIAQEQRAQEIAEIRGDMVEAAWGNQIRNYVFHPY
QMVKDLRTNHETTDVDGVMDGDLDAFIEASLRQDTWVGD
>Mature_358_residues
SKRTWGKPRTIFDLKTLDAKIFDLEQQAAQPEFWEDQQGAQDILQELTELKGTVAQYQQWQGQFEDAAAIAELLTEAEDP
ALLTEAMENLDDLQKELNRWELQQMLSGEYDNLGAVLTINAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVRLAEISEGDE
AGLKSATLEVTGRYAFGYLKHEKGTHRLVRISPFNANGKRQTSFAGVEVMPILGDNAVKVDIPEKDLEITTSRAGGKGGQ
NVNKVETAVRIVHLPTGVAVRCTQERSQLQNKEKALAILKAKLLIIAQEQRAQEIAEIRGDMVEAAWGNQIRNYVFHPYQ
MVKDLRTNHETTDVDGVMDGDLDAFIEASLRQDTWVGD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=300, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI34577120, Length=316, Percent_Identity=31.6455696202532, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI166795305, Length=167, Percent_Identity=33.5329341317365, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=43.3234421364985, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787462, Length=346, Percent_Identity=32.3699421965318, Blast_Score=160, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17542784, Length=276, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321295, Length=322, Percent_Identity=29.8136645962733, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI19921226, Length=270, Percent_Identity=36.2962962962963, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40256; Mature: 40125

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRTWGKPRTIFDLKTLDAKIFDLEQQAAQPEFWEDQQGAQDILQELTELKGTVAQYQQ
CCCCCCCCCCEEEEHHHHCHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
WQGQFEDAAAIAELLTEAEDPALLTEAMENLDDLQKELNRWELQQMLSGEYDNLGAVLTI
HCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
NAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVRLAEISEGDEAGLKSATLEVTGRYAFGYL
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCEEEEEE
KHEKGTHRLVRISPFNANGKRQTSFAGVEVMPILGDNAVKVDIPEKDLEITTSRAGGKGG
ECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCEEEECCCCCCCC
QNVNKVETAVRIVHLPTGVAVRCTQERSQLQNKEKALAILKAKLLIIAQEQRAQEIAEIR
CCHHHHHHHEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHH
GDMVEAAWGNQIRNYVFHPYQMVKDLRTNHETTDVDGVMDGDLDAFIEASLRQDTWVGD
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKRTWGKPRTIFDLKTLDAKIFDLEQQAAQPEFWEDQQGAQDILQELTELKGTVAQYQQ
CCCCCCCCCEEEEHHHHCHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
WQGQFEDAAAIAELLTEAEDPALLTEAMENLDDLQKELNRWELQQMLSGEYDNLGAVLTI
HCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
NAGAGGTDAQDWAEMLLRMYTRWSEKQGYKVRLAEISEGDEAGLKSATLEVTGRYAFGYL
ECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCEEEEEE
KHEKGTHRLVRISPFNANGKRQTSFAGVEVMPILGDNAVKVDIPEKDLEITTSRAGGKGG
ECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCEEEECCCCCCCC
QNVNKVETAVRIVHLPTGVAVRCTQERSQLQNKEKALAILKAKLLIIAQEQRAQEIAEIR
CCHHHHHHHEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHH
GDMVEAAWGNQIRNYVFHPYQMVKDLRTNHETTDVDGVMDGDLDAFIEASLRQDTWVGD
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]