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The map label for this gene is yibK [C]

Identifier: 169833898

GI number: 169833898

Start: 547687

End: 548190

Strand: Direct

Name: yibK [C]

Synonym: SPH_0598

Alternate gene names: 169833898

Gene position: 547687-548190 (Clockwise)

Preceding gene: 169833477

Following gene: 169834355

Centisome position: 24.39

GC content: 42.46

Gene sequence:

>504_bases
ATGACAAATCACATTGTATTATTTGAACCTCAAATTCCACAAAATACGGGCAATATCGCGCGTACTTGCGCTGCGACCAA
TTCTCCCCTCCACATCATCAAGCCTATGGGCTTTCCTATTGATGACCGCAAGATGAAGCGGGCTGGATTGGATTACTGGG
ATAAGCTAGAGATTTATTTTTACGAGAGTTTGGAAGATTTCATGTCTCAGATGAAGGGAAAACTTTATCTGATTTCGAAA
TTCGCGGAAAAAGTCTATTCTGAGGTGGATTTATCGACTGACGAGGACCATTATTTTCTCTTTGGACGTGAAGACAAGGG
CTTACCTGAAGATTTTATGCGAGAACATCCTGAGAAGGCTCTCCGTATTCCCATGAATGATGAACATGTCCGCAGTCTCA
ATGTGTCTAATACCGTCTGCATGATTGTCTATGAGGCTCTTCGCCAGCAGAACTTTGCAGGTCTTGAGCTCGTCCATACC
TATGAAGTGGATAAATTGAAATAA

Upstream 100 bases:

>100_bases
CTGTTGTGAGGTAAGTTAACGATGCCTTAACCACGCTGTTTGCTGAGCTTGACTCCGGGCAGTGTGGCTATTTTTTTGCA
ATGGTGAAAGGAAGCAAGTC

Downstream 100 bases:

>100_bases
AAAATGAAAATGAACAAAATGCTTGCGCTTGCAAGCGTTTTTTGTTATGATAAAAGAGTCTTCAGGGCAGGGTGTGATTC
CCGACCGGCGGTGACTTTAA

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRKMKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISK
FAEKVYSEVDLSTDEDHYFLFGREDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
YEVDKLK

Sequences:

>Translated_167_residues
MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRKMKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISK
FAEKVYSEVDLSTDEDHYFLFGREDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
YEVDKLK
>Mature_166_residues
TNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRKMKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKF
AEKVYSEVDLSTDEDHYFLFGREDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHTY
EVDKLK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=41.025641025641, Blast_Score=117, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19556; Mature: 19424

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRKMKRAGLDYWDKLEIYF
CCCCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCCCCCHHEEEEE
YESLEDFMSQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFGREDKGLPEDFMREHPEKA
HHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHE
LRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHTYEVDKLK
EECCCCCHHHEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
TNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRKMKRAGLDYWDKLEIYF
CCCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHHHHCCCCCCHHEEEEE
YESLEDFMSQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFGREDKGLPEDFMREHPEKA
HHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHCCHHE
LRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHTYEVDKLK
EECCCCCHHHEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA