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The map label for this gene is thiE [H]

Identifier: 169833541

GI number: 169833541

Start: 757763

End: 758395

Strand: Direct

Name: thiE [H]

Synonym: SPH_0812

Alternate gene names: 169833541

Gene position: 757763-758395 (Clockwise)

Preceding gene: 169833318

Following gene: 169834494

Centisome position: 33.74

GC content: 43.76

Gene sequence:

>633_bases
ATGAATAGAGAAGCACTTAGACTATATCTGGTAACCAATCGCTATCAAGATTCCGTGGAAAGCTTTCTTGCAAAAGTTGA
GACGGCCTGCCGTTCAGGGGTTACCATAGTCCAATTGCGAGAAAAAAATCTCACAACCAATCAATATTATCAACTGGCAA
AACAAGTCAAGGAAATAACAGATGCTTATCAGGTACCCTTGATAATCGATGATCGGTTGGATGTTTGTCTTGCGGTTGAT
GCTGCTGGTCTGCATATTGGAGATGACGAACTCCCAGTTTCGGTTGCCCGACAAGTCTTGGGTCCTGAAAAAATCCTCGG
TGTCACCGCTAAAACGGTTAAAAGAGCTCTGGAAGCGGAGAAATCAGGTGCAGATTACTTGGGGACAGGAGCCATTTTTC
CGACAACCACCAAGGAAAATGCACCCATCACCCTGATTTCAACCTTGAAAACAATTTGCCAAACGGTTGCCATTCCAATA
GTTGCTATTGGCGGCTTGACTTCGGAGAATATTGATCAGCTTATGGGCACTGGCATAGCTGGTGTAGCTGTTGTACGTGA
TTTGATGCAAGCAGAAGATATTGAGGCAAAAACGCAAGCCTTTTTAACAAAGTTGCATGACATTCTTTCCTAA

Upstream 100 bases:

>100_bases
CAAGGAATGGGATTGGAAGAATTTCGTTACCAAGTACTCAATCAGCTTTCGCTCCTAAGAAGAGATGAAAATTGGCTAGA
TACCATCAAAGGAGAGGTTT

Downstream 100 bases:

>100_bases
TTAATACTCAATGAAAATCAAAGAGTAAACTAGAAAGCTAGTCGCAGGTTGCTCAAAGTACTGCCTTGAGGTTTTAGATA
AGACTGACGAAGTCAGCTCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase 2; TMP-PPase 2; Thiamine-phosphate synthase 2 [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVD
AAGLHIGDDELPVSVARQVLGPEKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS

Sequences:

>Translated_210_residues
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVD
AAGLHIGDDELPVSVARQVLGPEKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS
>Mature_210_residues
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVD
AAGLHIGDDELPVSVARQVLGPEKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=217, Percent_Identity=35.9447004608295, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22759; Mature: 22759

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEIT
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGPEKILGVTAKTVKRALEAE
HHHHCCEEECCCCCEEEEEECCCCEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPIVAIGGLTSENIDQLMGTGIA
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHH
GVAVVRDLMQAEDIEAKTQAFLTKLHDILS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEIT
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGPEKILGVTAKTVKRALEAE
HHHHCCEEECCCCCEEEEEECCCCEECCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHH
KSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPIVAIGGLTSENIDQLMGTGIA
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHH
GVAVVRDLMQAEDIEAKTQAFLTKLHDILS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]