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The map label for this gene is thiM2 [H]

Identifier: 169833318

GI number: 169833318

Start: 756967

End: 757770

Strand: Direct

Name: thiM2 [H]

Synonym: SPH_0811

Alternate gene names: 169833318

Gene position: 756967-757770 (Clockwise)

Preceding gene: 169832871

Following gene: 169833541

Centisome position: 33.71

GC content: 43.03

Gene sequence:

>804_bases
ATGCAGGAATTTACAAATCCCTTTCCTATAGGCTCTAGTTCCCTCATTCACTGTATGACCAATGAGATTTCTTGTGAGAT
GCTGGCAAATGGGATTTTGGCTCTGGGATGCAAACCTGTCATGGCAGATGATTCCCGTGAAGTTCTTGATTTTACTAAGC
AAAGTCAGGCTCTCTTCATCAATTTAGGTCATTTGTCAGCTGAGAAGGAGAAAGCAATCCGCATGGCAGCTTCGTATGCA
AACCAATCTTCTCTCCCAATGGTAGTAGATGCGGTTGGCGTAACGACTTCATCCATTCGTAAGAGCTTAGTTAAAGACCT
TTTAGACTATAGACCTACGGTCCTTAAAGGAAACATGTCAGAAATTCGAAGTCTTGTTGGATTAAAGCACCACGGCGTTG
GGGTCGATGCGAGTGCTAAAGATCAAGAAACGGAGGATTTGCTTCAAGTCTTGAAAGACTGGTGTCAGACCTATCCTGGT
ATGTCATTCTTAGTTACTGGCCCCAAGGACCTCATCGTTTCGAAAAATCAGGTCGCTGTACTGGAGAATGGCTGTACTGA
ATTAGACTGGATAACAGGGACAGGAGATTTGGTTGGAGCCTTAACAGCCGTTTTTCTCAGCCAAGGAAAGACTGGTTTTG
AAGCTTCTTGCTTAGCAGTCTCTTATCTCAATATCGCTGCTGAGAAAATAGTTGTTCAAGGAATGGGATTGGAAGAATTT
CGTTACCAAGTACTCAATCAGCTTTCGCTCCTAAGAAGAGATGAAAATTGGCTAGATACCATCAAAGGAGAGGTTTATGA
ATAG

Upstream 100 bases:

>100_bases
TGATTGGTACAGCGATTTCCTTTATTGCCTTTCGATTTTTAATCAAGCAGGAATTCTTTAAAAAAGTGCAGGGATATTTC
TTTAGTGAAAGGATAGACTG

Downstream 100 bases:

>100_bases
AGAAGCACTTAGACTATATCTGGTAACCAATCGCTATCAAGATTCCGTGGAAAGCTTTCTTGCAAAAGTTGAGACGGCCT
GCCGTTCAGGGGTTACCATA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase 2; TH kinase 2; Thz kinase 2 [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADDSREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYA
NQSSLPMVVDAVGVTTSSIRKSLVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCLAVSYLNIAAEKIVVQGMGLEEF
RYQVLNQLSLLRRDENWLDTIKGEVYE

Sequences:

>Translated_267_residues
MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADDSREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYA
NQSSLPMVVDAVGVTTSSIRKSLVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCLAVSYLNIAAEKIVVQGMGLEEF
RYQVLNQLSLLRRDENWLDTIKGEVYE
>Mature_267_residues
MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADDSREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYA
NQSSLPMVVDAVGVTTSSIRKSLVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
MSFLVTGPKDLIVSKNQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCLAVSYLNIAAEKIVVQGMGLEEF
RYQVLNQLSLLRRDENWLDTIKGEVYE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=242, Percent_Identity=28.9256198347107, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325042, Length=301, Percent_Identity=25.5813953488372, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 29186; Mature: 29186

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADDSREVLDFTKQSQALFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHCCCCEEEE
NLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKSLVKDLLDYRPTVLKGNMS
EECCCCHHHHHHHHHHHHHCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHCCCCEEECCHH
EIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPGMSFLVTGPKDLIVSKNQVAV
HHHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHEECCCCEEE
LENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCLAVSYLNIAAEKIVVQGMGLEEF
EECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
RYQVLNQLSLLRRDENWLDTIKGEVYE
HHHHHHHHHHHHCCCHHHHHHCCCCCC
>Mature Secondary Structure
MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADDSREVLDFTKQSQALFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHCCCCEEEE
NLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKSLVKDLLDYRPTVLKGNMS
EECCCCHHHHHHHHHHHHHCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHCCCCEEECCHH
EIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPGMSFLVTGPKDLIVSKNQVAV
HHHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHEECCCCEEE
LENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCLAVSYLNIAAEKIVVQGMGLEEF
EECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
RYQVLNQLSLLRRDENWLDTIKGEVYE
HHHHHHHHHHHHCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA