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The map label for this gene is miaA

Identifier: 169824442

GI number: 169824442

Start: 810919

End: 811863

Strand: Direct

Name: miaA

Synonym: FMG_0745

Alternate gene names: 169824442

Gene position: 810919-811863 (Clockwise)

Preceding gene: 169824441

Following gene: 169824443

Centisome position: 45.11

GC content: 28.25

Gene sequence:

>945_bases
ATGAGGAAAAAATGTATTGTAATAGTTGGACCTACTGGAGTGGGGAAGACCAGGTTGAGTATTTTTTTAGCAAAAAGATT
AAGTAGTGAGATTATTTCTGCAGATTCAATGCAAATATATAAGTATATGGATATTGGAACTGCAAAAGTAGAACCAAAAT
ATCAGAGAGTAATTAAACATCATTTAATAGATATAGTTGAACCATATGAAAATTTTAATGTTGAACAATTTAAAAATCTA
TGCATAGAAAAAATTGAAGAAATTTCATCAAAAAACAAAATCCCAATTATTGTTGGAGGCACAGGCTTATATATAAACTC
GATAACTCATAAGTTAGAGTTTAATGCTGTTAAATCTGATGACAAATTAAGAGAAGAATTGGAAAATATTTCTTTACAAT
ATGGAAATGAGAAACTTCATGAAATATTAGAAGATATAGATCCAAAGTCCGCTGATAAGATTCACATGAACAACGTGAGA
CGTGTTATTAGAGCAATCGAAGTATGTAAACTAACTGGGCATAAGTTTAGTGAAATTAACGATAAATTTGACCATTATAA
CGATGATTATGATTTTTATATAATTGGATTAAATGATGACAGACAGGTCCTTTACGAAAGGATTAACAAAAGAGTTGATG
AGATGATTGATGAAGGTTTTATGGCTGAATGCAAATACATTTATGAAATGACGGATGAAAACTCTCAATCAATTCAAGCT
ATAGGCTATAGAGAAGCTTTTATGTATTTAAATAATAAGATATCTTTTAAAGATATGATTAGTCTAATGAAAAAAAATAG
TAGAAAATATGCTAAGAGACAGTTAACGTGGTTTAGACAAGATAAAAGAATTCATTGGATGAATTTGAAAGACTTTAGAG
AATTTGAAGACATCGAACAAATTTGTTTGAAAAATGTGAAAGAATGGTTATATGATAAGAGATAA

Upstream 100 bases:

>100_bases
ATTTGTTTAAATGTGAATATCCATTAACATGTCCTCATGGAAGGCCAACTTTTATTGAAATGAATGAAAAAGATCTAGAA
AAAATGTTTATGAGGATAAA

Downstream 100 bases:

>100_bases
AATAAAAGAATGGTACGGTATAGATGATTATAGTTTTGATACAGTTATGGAAAGTGATTATCTACTATCCGAAAAATTTA
AAGAATTAGAAAACTTGGAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRKKCIVIVGPTGVGKTRLSIFLAKRLSSEIISADSMQIYKYMDIGTAKVEPKYQRVIKHHLIDIVEPYENFNVEQFKNL
CIEKIEEISSKNKIPIIVGGTGLYINSITHKLEFNAVKSDDKLREELENISLQYGNEKLHEILEDIDPKSADKIHMNNVR
RVIRAIEVCKLTGHKFSEINDKFDHYNDDYDFYIIGLNDDRQVLYERINKRVDEMIDEGFMAECKYIYEMTDENSQSIQA
IGYREAFMYLNNKISFKDMISLMKKNSRKYAKRQLTWFRQDKRIHWMNLKDFREFEDIEQICLKNVKEWLYDKR

Sequences:

>Translated_314_residues
MRKKCIVIVGPTGVGKTRLSIFLAKRLSSEIISADSMQIYKYMDIGTAKVEPKYQRVIKHHLIDIVEPYENFNVEQFKNL
CIEKIEEISSKNKIPIIVGGTGLYINSITHKLEFNAVKSDDKLREELENISLQYGNEKLHEILEDIDPKSADKIHMNNVR
RVIRAIEVCKLTGHKFSEINDKFDHYNDDYDFYIIGLNDDRQVLYERINKRVDEMIDEGFMAECKYIYEMTDENSQSIQA
IGYREAFMYLNNKISFKDMISLMKKNSRKYAKRQLTWFRQDKRIHWMNLKDFREFEDIEQICLKNVKEWLYDKR
>Mature_314_residues
MRKKCIVIVGPTGVGKTRLSIFLAKRLSSEIISADSMQIYKYMDIGTAKVEPKYQRVIKHHLIDIVEPYENFNVEQFKNL
CIEKIEEISSKNKIPIIVGGTGLYINSITHKLEFNAVKSDDKLREELENISLQYGNEKLHEILEDIDPKSADKIHMNNVR
RVIRAIEVCKLTGHKFSEINDKFDHYNDDYDFYIIGLNDDRQVLYERINKRVDEMIDEGFMAECKYIYEMTDENSQSIQA
IGYREAFMYLNNKISFKDMISLMKKNSRKYAKRQLTWFRQDKRIHWMNLKDFREFEDIEQICLKNVKEWLYDKR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=34.0836012861736, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI1790613, Length=289, Percent_Identity=33.9100346020761, Blast_Score=187, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI25144712, Length=285, Percent_Identity=32.280701754386, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=34.1296928327645, Blast_Score=159, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24649830, Length=357, Percent_Identity=31.3725490196078, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_FINM2 (B0S1C3)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001692053.1
- ProteinModelPortal:   B0S1C3
- SMR:   B0S1C3
- GeneID:   6020092
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0745
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- BioCyc:   FMAG334413:FMG_0745-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 37373; Mature: 37373

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKCIVIVGPTGVGKTRLSIFLAKRLSSEIISADSMQIYKYMDIGTAKVEPKYQRVIKH
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHHHHH
HLIDIVEPYENFNVEQFKNLCIEKIEEISSKNKIPIIVGGTGLYINSITHKLEFNAVKSD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHEEECCCCCH
DKLREELENISLQYGNEKLHEILEDIDPKSADKIHMNNVRRVIRAIEVCKLTGHKFSEIN
HHHHHHHHHCHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
DKFDHYNDDYDFYIIGLNDDRQVLYERINKRVDEMIDEGFMAECKYIYEMTDENSQSIQA
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
IGYREAFMYLNNKISFKDMISLMKKNSRKYAKRQLTWFRQDKRIHWMNLKDFREFEDIEQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
ICLKNVKEWLYDKR
HHHHHHHHHHCCCC
>Mature Secondary Structure
MRKKCIVIVGPTGVGKTRLSIFLAKRLSSEIISADSMQIYKYMDIGTAKVEPKYQRVIKH
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHHHHH
HLIDIVEPYENFNVEQFKNLCIEKIEEISSKNKIPIIVGGTGLYINSITHKLEFNAVKSD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHEEECCCCCH
DKLREELENISLQYGNEKLHEILEDIDPKSADKIHMNNVRRVIRAIEVCKLTGHKFSEIN
HHHHHHHHHCHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
DKFDHYNDDYDFYIIGLNDDRQVLYERINKRVDEMIDEGFMAECKYIYEMTDENSQSIQA
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHH
IGYREAFMYLNNKISFKDMISLMKKNSRKYAKRQLTWFRQDKRIHWMNLKDFREFEDIEQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
ICLKNVKEWLYDKR
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA