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The map label for this gene is fadD25 [H]

Identifier: 169630659

GI number: 169630659

Start: 3625447

End: 3627192

Strand: Reverse

Name: fadD25 [H]

Synonym: MAB_3579c

Alternate gene names: 169630659

Gene position: 3627192-3625447 (Counterclockwise)

Preceding gene: 169630660

Following gene: 169630658

Centisome position: 71.58

GC content: 65.41

Gene sequence:

>1746_bases
ATGCAGACGACGTACGATTTCACCGACTACTTCAGCACCGTCACCGAATGGGTTGCCCGACAACCGGATCAGATTGCCCT
GGGCTACCTGGAAGAAGGTGCGGGCGAACTGACGGAGCGCGCGTGGACCTACGCCGAACTCGTAAGTCATGCCACCGCCG
TGGCCGCACGTCTTCGTGAAGACCTGGAGCCCGGGGACCGTACCCTGCTGCTGTATCCGCCCGGGCTACCCTTTGTCGCG
GCGTTTCTTGGCTGCTTGGCTGCCGGAGTGGTTGCGGTTCCCGCTCCACTGCCCGATGGTCGTGACAAGGCGCAGCACCG
TCTTGCCGGCATCATCGCGGACGCGGGCACACGCGTTGTGCTGACGGAGTCGTCGGTTGCGCCACTTATCGCGGGCTGGG
CCGACGCGCTGCCCACGCCGATCGCGCTCATCGCCACCGATGCGATTCCCGTCGAGCTGGGGCGCGAATTCGTTCCGGTG
CGGTCGGCGCCTTCCGATACCGCCTTCCTGCAGTACACGTCGGGTTCGACCAATGCCCCGAAGGGTGTGATCGTCACCCA
CGGGAACCTCGTCCACAACGAGCAGGAACTCGGAGCCCGCATCGAGAATGACCCGTCGTTCTCGATGCTCAGCTGGCTGC
CGCAGTTTCATGACATGGGCCTGATCGGCATGCTGCTCTGGCCGTTATTCTACGGCGGCACTTCGCGTTTCCTGTCACCT
GTGACGTTTATCAAGCATCCCATCCAGTGGATCCGGGCCATGTCGAAGTACCGGGCGACCGTAGGAGTGGCACCGAACTT
CGCCTTCGACCTGGTGGCACGGCGCTGCACGCACGAGGCGCTCGAGGGTGTCGATCTGTCGTGCGTACGGTCGCTACTCA
ACGGATCGGAGCCCGTGCAGTGGGCTTCTATCGAGCGGTTCACCACGCTGCTGGCTCCGTTCGGACTCGCGGAGCATGCG
GTTACCCCGGTGTATGGGCTTGCCGAAGCCACGTTGTTCGTGACCGGCGATCCGCACGGCACGCCGGTCACCACCGCCGT
TGTCGACGGTGAGGCGCTTGCCGAAAATCGGTTGGTGGTCGCCAACGAAGGCGCGCCACGGTCCCGAGTGCTGGTTGCCA
GCGGCCGCATCGGTGGTATCGAAGTTTCGGTCGTCGGAGCCGACGGTGCACCTCTGCCCGACGGCGAAATCGGCGAGATC
TGGCTGCGCGGAACGAGTGTGGCCGCTGGGTATTGGAACCTTCCGGAGTTGTCCGCCGAGATATTCGGCGCGACACCGAT
CGGCGGCACGGGCGGCTGGTTGCGTACCGGGGACCTGGGCACCCTGGTCGATGGCAACCTCTTCATCACCGGTCGCACCA
AGGACGTCATCATTGTCAACGGCCGCAATCTCTACGCCCATGACATCGAGGCGGCCGCGCGTGCGGCCGCCCCGGCCCTG
GCACGCGGGGTGGGCGCGGCATTCGCGGTCGGCACCCCTGAGCGGCTCGTGCTGGTTCACGAGATCCGCCCGGAGAATCT
TGGTGATGCCTCCCTTGACGACGTGCTCGCGGCGGTACGCACCGCGATATTCGCCGAATTCGCCACCCCGCTGAGCGATT
TGGTGCTGGTGGCCAAGGGGGAGGTAGCCCGTACCACCAGTGGCAAGATCCGTAGAACCCATATGCGAGCCTCCTATGAG
GGGGGCCGGATCACGGCGTTCGCGGATCCGCGGCTGGCCTCCGCCGGCGGGGACGTCGTCCGGTGA

Upstream 100 bases:

>100_bases
TTCGCTACTTAGGCGTCGCTGAAGGGCGTCCGGCTGTGCAGCGGGTGCCGATTATTGCGAGCCGGGCCAAGTCAGAGGAA
ACGGAAGGGCAGAAAGCAAC

Downstream 100 bases:

>100_bases
CGACATCGATCACGCTCGTGGCACCGCGACGCTTCGACATTGTCGACACGGTCGAGGCCGCGCTGGGCGATGCCCGCGAC
CCGGACAACCCGGCTGGTTT

Product: putative acyl-CoA synthase/polyketide synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MQTTYDFTDYFSTVTEWVARQPDQIALGYLEEGAGELTERAWTYAELVSHATAVAARLREDLEPGDRTLLLYPPGLPFVA
AFLGCLAAGVVAVPAPLPDGRDKAQHRLAGIIADAGTRVVLTESSVAPLIAGWADALPTPIALIATDAIPVELGREFVPV
RSAPSDTAFLQYTSGSTNAPKGVIVTHGNLVHNEQELGARIENDPSFSMLSWLPQFHDMGLIGMLLWPLFYGGTSRFLSP
VTFIKHPIQWIRAMSKYRATVGVAPNFAFDLVARRCTHEALEGVDLSCVRSLLNGSEPVQWASIERFTTLLAPFGLAEHA
VTPVYGLAEATLFVTGDPHGTPVTTAVVDGEALAENRLVVANEGAPRSRVLVASGRIGGIEVSVVGADGAPLPDGEIGEI
WLRGTSVAAGYWNLPELSAEIFGATPIGGTGGWLRTGDLGTLVDGNLFITGRTKDVIIVNGRNLYAHDIEAAARAAAPAL
ARGVGAAFAVGTPERLVLVHEIRPENLGDASLDDVLAAVRTAIFAEFATPLSDLVLVAKGEVARTTSGKIRRTHMRASYE
GGRITAFADPRLASAGGDVVR

Sequences:

>Translated_581_residues
MQTTYDFTDYFSTVTEWVARQPDQIALGYLEEGAGELTERAWTYAELVSHATAVAARLREDLEPGDRTLLLYPPGLPFVA
AFLGCLAAGVVAVPAPLPDGRDKAQHRLAGIIADAGTRVVLTESSVAPLIAGWADALPTPIALIATDAIPVELGREFVPV
RSAPSDTAFLQYTSGSTNAPKGVIVTHGNLVHNEQELGARIENDPSFSMLSWLPQFHDMGLIGMLLWPLFYGGTSRFLSP
VTFIKHPIQWIRAMSKYRATVGVAPNFAFDLVARRCTHEALEGVDLSCVRSLLNGSEPVQWASIERFTTLLAPFGLAEHA
VTPVYGLAEATLFVTGDPHGTPVTTAVVDGEALAENRLVVANEGAPRSRVLVASGRIGGIEVSVVGADGAPLPDGEIGEI
WLRGTSVAAGYWNLPELSAEIFGATPIGGTGGWLRTGDLGTLVDGNLFITGRTKDVIIVNGRNLYAHDIEAAARAAAPAL
ARGVGAAFAVGTPERLVLVHEIRPENLGDASLDDVLAAVRTAIFAEFATPLSDLVLVAKGEVARTTSGKIRRTHMRASYE
GGRITAFADPRLASAGGDVVR
>Mature_581_residues
MQTTYDFTDYFSTVTEWVARQPDQIALGYLEEGAGELTERAWTYAELVSHATAVAARLREDLEPGDRTLLLYPPGLPFVA
AFLGCLAAGVVAVPAPLPDGRDKAQHRLAGIIADAGTRVVLTESSVAPLIAGWADALPTPIALIATDAIPVELGREFVPV
RSAPSDTAFLQYTSGSTNAPKGVIVTHGNLVHNEQELGARIENDPSFSMLSWLPQFHDMGLIGMLLWPLFYGGTSRFLSP
VTFIKHPIQWIRAMSKYRATVGVAPNFAFDLVARRCTHEALEGVDLSCVRSLLNGSEPVQWASIERFTTLLAPFGLAEHA
VTPVYGLAEATLFVTGDPHGTPVTTAVVDGEALAENRLVVANEGAPRSRVLVASGRIGGIEVSVVGADGAPLPDGEIGEI
WLRGTSVAAGYWNLPELSAEIFGATPIGGTGGWLRTGDLGTLVDGNLFITGRTKDVIIVNGRNLYAHDIEAAARAAAPAL
ARGVGAAFAVGTPERLVLVHEIRPENLGDASLDDVLAAVRTAIFAEFATPLSDLVLVAKGEVARTTSGKIRRTHMRASYE
GGRITAFADPRLASAGGDVVR

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI45827692, Length=586, Percent_Identity=26.7918088737201, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI225735629, Length=586, Percent_Identity=26.7918088737201, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI44888818, Length=501, Percent_Identity=26.5469061876248, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI55749758, Length=502, Percent_Identity=25.2988047808765, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI225735625, Length=551, Percent_Identity=25.4083484573503, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI45827694, Length=551, Percent_Identity=25.4083484573503, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI45827696, Length=512, Percent_Identity=24.4140625, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI225735627, Length=512, Percent_Identity=24.4140625, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI187761345, Length=544, Percent_Identity=26.2867647058824, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI187761343, Length=544, Percent_Identity=26.2867647058824, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI45827698, Length=473, Percent_Identity=23.8900634249471, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1788107, Length=517, Percent_Identity=24.5647969052224, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI193202679, Length=611, Percent_Identity=25.8592471358429, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17558820, Length=561, Percent_Identity=26.5597147950089, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=485, Percent_Identity=23.9175257731959, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=557, Percent_Identity=22.9802513464991, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI18859661, Length=554, Percent_Identity=24.3682310469314, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24654656, Length=456, Percent_Identity=25, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24581924, Length=560, Percent_Identity=25.7142857142857, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 61577; Mature: 61577

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTYDFTDYFSTVTEWVARQPDQIALGYLEEGAGELTERAWTYAELVSHATAVAARLRE
CCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLEPGDRTLLLYPPGLPFVAAFLGCLAAGVVAVPAPLPDGRDKAQHRLAGIIADAGTRVV
HCCCCCCEEEEECCCHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHEECCCCEEE
LTESSVAPLIAGWADALPTPIALIATDAIPVELGREFVPVRSAPSDTAFLQYTSGSTNAP
EECCCCCHHHHHHHHHCCCCEEEEEECCEEHHHCCCEEECCCCCCCCEEEEECCCCCCCC
KGVIVTHGNLVHNEQELGARIENDPSFSMLSWLPQFHDMGLIGMLLWPLFYGGTSRFLSP
CEEEEECCCEECCHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHH
VTFIKHPIQWIRAMSKYRATVGVAPNFAFDLVARRCTHEALEGVDLSCVRSLLNGSEPVQ
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
WASIERFTTLLAPFGLAEHAVTPVYGLAEATLFVTGDPHGTPVTTAVVDGEALAENRLVV
HHHHHHHHHHHHCCCCHHHHCCCHHCCCEEEEEEECCCCCCCEEEEEECCHHHHCCCEEE
ANEGAPRSRVLVASGRIGGIEVSVVGADGAPLPDGEIGEIWLRGTSVAAGYWNLPELSAE
ECCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEECCEEECCCCCCCHHHHH
IFGATPIGGTGGWLRTGDLGTLVDGNLFITGRTKDVIIVNGRNLYAHDIEAAARAAAPAL
HCCCCCCCCCCCCEEECCCCEEECCCEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHH
ARGVGAAFAVGTPERLVLVHEIRPENLGDASLDDVLAAVRTAIFAEFATPLSDLVLVAKG
HHHCCCEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHEEEEECC
EVARTTSGKIRRTHMRASYEGGRITAFADPRLASAGGDVVR
CEEECCCCCEEEEEEHEECCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQTTYDFTDYFSTVTEWVARQPDQIALGYLEEGAGELTERAWTYAELVSHATAVAARLRE
CCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLEPGDRTLLLYPPGLPFVAAFLGCLAAGVVAVPAPLPDGRDKAQHRLAGIIADAGTRVV
HCCCCCCEEEEECCCHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHEECCCCEEE
LTESSVAPLIAGWADALPTPIALIATDAIPVELGREFVPVRSAPSDTAFLQYTSGSTNAP
EECCCCCHHHHHHHHHCCCCEEEEEECCEEHHHCCCEEECCCCCCCCEEEEECCCCCCCC
KGVIVTHGNLVHNEQELGARIENDPSFSMLSWLPQFHDMGLIGMLLWPLFYGGTSRFLSP
CEEEEECCCEECCHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHH
VTFIKHPIQWIRAMSKYRATVGVAPNFAFDLVARRCTHEALEGVDLSCVRSLLNGSEPVQ
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
WASIERFTTLLAPFGLAEHAVTPVYGLAEATLFVTGDPHGTPVTTAVVDGEALAENRLVV
HHHHHHHHHHHHCCCCHHHHCCCHHCCCEEEEEEECCCCCCCEEEEEECCHHHHCCCEEE
ANEGAPRSRVLVASGRIGGIEVSVVGADGAPLPDGEIGEIWLRGTSVAAGYWNLPELSAE
ECCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEECCEEECCCCCCCHHHHH
IFGATPIGGTGGWLRTGDLGTLVDGNLFITGRTKDVIIVNGRNLYAHDIEAAARAAAPAL
HCCCCCCCCCCCCEEECCCCEEECCCEEEEECCCEEEEECCCEEEEHHHHHHHHHHHHHH
ARGVGAAFAVGTPERLVLVHEIRPENLGDASLDDVLAAVRTAIFAEFATPLSDLVLVAKG
HHHCCCEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHEEEEECC
EVARTTSGKIRRTHMRASYEGGRITAFADPRLASAGGDVVR
CEEECCCCCEEEEEEHEECCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]