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The map label for this gene is ilvB1 [H]

Identifier: 169630404

GI number: 169630404

Start: 3368087

End: 3369931

Strand: Reverse

Name: ilvB1 [H]

Synonym: MAB_3323c

Alternate gene names: 169630404

Gene position: 3369931-3368087 (Counterclockwise)

Preceding gene: 169630406

Following gene: 169630403

Centisome position: 66.51

GC content: 66.4

Gene sequence:

>1845_bases
ATGAGCGCTCCCGCAAAGCCCAAGCCGGCTTCTGCTTCTGGGCGGGCCCCAGGAACAGCCCCGGAACCCAACACCGTCGC
ACCCCAGCGCGTCACCGGCGCGCAGTCGGTAGTGCGGTCGCTTGAGGAGCTCGGCGTCGAAGTGATCTTCGGCATTCCCG
GTGGTGCGGTCCTGCCCGTCTACGACCCGCTCTACGACTCCACCAAGGTGCGCCACGTGCTGGTGCGTCATGAGCAGGGT
GCCGGGCATGCCGCCAGCGGTTACGCGCATGCCAACGGCAAGGTCGGCGTCATGATGGCCACCTCCGGCCCCGGTGCCAC
CAACCTGGTCACCCCGCTGGCCGACGCGCAGATGGACTCGATCCCGGTTGTGGCGATCACCGGCCAGGTGGGTCGCGGCC
TCATCGGTACGGATGCTTTCCAGGAAGCCGACATCTCCGGCATCACGATGCCCATCACCAAGCACAACTTCCTGGTGAGC
AACGCCGAGGACATCCCGAAGGTGATCGCCGAGGCCTTCTACATCGCGCAGAGCGGCCGTCCCGGTGCGGTGCTCGTGGA
CATCCCCAAGGACATCCTGCAGGCACAGACCACGTTCAGCTGGCCGCCGCAGATCTACCTGCCCGGCTACAAGCCGGTCA
CCAAGCCGCACGGCAAGCAGGTTCGTGAGGCGGTCCGGCTCATCGCCGCCGCCAAGCGCCCGGTGCTGTACGTCGGCGGC
GGTGTCATCCGTGCGGACGCCTCCCCGGAACTCAAGGAATTGGCCGAACTGACCGGCATCCCCGTCGTCACCACCCTTAT
GGCGCGCGGTGCATTCCCCGACAGCCACGAGCTGCACATGGGTATGCCCGGGATGCACGGCACGGTCGGTGCGGTGGCCG
CACTGCAGCGCAGCGATCTGCTGATCACCCTGGGTGCCCGATTCGATGACCGGGTTACCGGGCAGCTGGACTCGTTTGCG
CCGGAGGCCAAGGTGATTCACGCCGACATCGACCCGGCCGAGATCGGTAAGAACCGGCATGCCGATGTGCCGATCGTCGG
CGACGTGAAGAACGTCATCATCGAGCTGATCGAGGCGATCCGGGCGGACCGCGCTGCCGGCACGGTGGCCGATCCTCTCT
CCGAATGGTGGGAGTACCTGTCGGATGTCCGCAAGACGTATCCGCTGGGATACGACGCGCAGCACGACGGCAGCCTGGGC
CCGGAGTTCGTCATCGAAACGCTGGGCAAGATCGCCGGACCCGACGCGGTGTACGTGGCGGGTGTGGGTCAGCACCAGAT
GTGGGCCGCGCAGTTCATCAAATACGAGAACCCGCGCACCTGGCTCAACTCCGGGGGCCTGGGCACCATGGGCTTCGCGG
TGCCCGCCGCCATGGGCGCCAAGATGGCGCGGCCCGAGGCCGAGGTGTGGGCCATCGACGGTGACGGCTGCTTCCAGATG
ACCAACCAGGAGCTGGCGACCTGCGCCATCGAGGGTGTGCCCATCAAGGTCGCGCTCATCAACAACGGCAATCTGGGCAT
GGTGCGCCAGTGGCAGACCCTGTTCTACGAGGAGCGCTACTCGCAGACGGATCTGGCGACACACTCGCGCCGCATCCCCG
ATTTCGTGAAGCTGGCCGAGGCGCTGGGTTGTGTCGGATTGCGTTGCGAGCGTGAAGAAGACGTGGCCAAGGTGATCGAA
GAGGCGCGCGCCATCAATGACCGCCCGGTGGTCATCGACTTCATCGTCGGTGCCGACGCTCAGGTGTGGCCCATGGTCGC
CGCCGGCACCAGCAATGACGAGATCCAGGCGGCCCGGGGCATCCGGCCGCTGTTCGACGACGACGAATTGGTGGCAGACG
AATGA

Upstream 100 bases:

>100_bases
GCGCACACTCGACGCGCAACCCTCGTACAGCAGACCGCTGGCGGGGGTTTTTTGTTGCCATAGGTTTTCAGTACGGCCAC
AACAAGAGGACAGACCACCA

Downstream 100 bases:

>100_bases
CAAGTACCCACACCCTGAGCGTTCTGGTCGAGGACCGTCCCGGTGTGCTCGCCCGCGTCGCCGCACTGTTTTCCCGCCGT
GGCTTCAACATCGCCTCGCT

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MSAPAKPKPASASGRAPGTAPEPNTVAPQRVTGAQSVVRSLEELGVEVIFGIPGGAVLPVYDPLYDSTKVRHVLVRHEQG
AGHAASGYAHANGKVGVMMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLVS
NAEDIPKVIAEAFYIAQSGRPGAVLVDIPKDILQAQTTFSWPPQIYLPGYKPVTKPHGKQVREAVRLIAAAKRPVLYVGG
GVIRADASPELKELAELTGIPVVTTLMARGAFPDSHELHMGMPGMHGTVGAVAALQRSDLLITLGARFDDRVTGQLDSFA
PEAKVIHADIDPAEIGKNRHADVPIVGDVKNVIIELIEAIRADRAAGTVADPLSEWWEYLSDVRKTYPLGYDAQHDGSLG
PEFVIETLGKIAGPDAVYVAGVGQHQMWAAQFIKYENPRTWLNSGGLGTMGFAVPAAMGAKMARPEAEVWAIDGDGCFQM
TNQELATCAIEGVPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSRRIPDFVKLAEALGCVGLRCEREEDVAKVIE
EARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDDELVADE

Sequences:

>Translated_614_residues
MSAPAKPKPASASGRAPGTAPEPNTVAPQRVTGAQSVVRSLEELGVEVIFGIPGGAVLPVYDPLYDSTKVRHVLVRHEQG
AGHAASGYAHANGKVGVMMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLVS
NAEDIPKVIAEAFYIAQSGRPGAVLVDIPKDILQAQTTFSWPPQIYLPGYKPVTKPHGKQVREAVRLIAAAKRPVLYVGG
GVIRADASPELKELAELTGIPVVTTLMARGAFPDSHELHMGMPGMHGTVGAVAALQRSDLLITLGARFDDRVTGQLDSFA
PEAKVIHADIDPAEIGKNRHADVPIVGDVKNVIIELIEAIRADRAAGTVADPLSEWWEYLSDVRKTYPLGYDAQHDGSLG
PEFVIETLGKIAGPDAVYVAGVGQHQMWAAQFIKYENPRTWLNSGGLGTMGFAVPAAMGAKMARPEAEVWAIDGDGCFQM
TNQELATCAIEGVPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSRRIPDFVKLAEALGCVGLRCEREEDVAKVIE
EARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDDELVADE
>Mature_613_residues
SAPAKPKPASASGRAPGTAPEPNTVAPQRVTGAQSVVRSLEELGVEVIFGIPGGAVLPVYDPLYDSTKVRHVLVRHEQGA
GHAASGYAHANGKVGVMMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLVSN
AEDIPKVIAEAFYIAQSGRPGAVLVDIPKDILQAQTTFSWPPQIYLPGYKPVTKPHGKQVREAVRLIAAAKRPVLYVGGG
VIRADASPELKELAELTGIPVVTTLMARGAFPDSHELHMGMPGMHGTVGAVAALQRSDLLITLGARFDDRVTGQLDSFAP
EAKVIHADIDPAEIGKNRHADVPIVGDVKNVIIELIEAIRADRAAGTVADPLSEWWEYLSDVRKTYPLGYDAQHDGSLGP
EFVIETLGKIAGPDAVYVAGVGQHQMWAAQFIKYENPRTWLNSGGLGTMGFAVPAAMGAKMARPEAEVWAIDGDGCFQMT
NQELATCAIEGVPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSRRIPDFVKLAEALGCVGLRCEREEDVAKVIEE
ARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDDELVADE

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=596, Percent_Identity=27.5167785234899, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI93004078, Length=578, Percent_Identity=26.8166089965398, Blast_Score=176, Evalue=6e-44,
Organism=Escherichia coli, GI87081685, Length=580, Percent_Identity=45.6896551724138, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI1790104, Length=574, Percent_Identity=44.7735191637631, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=37.7737226277372, Blast_Score=348, Evalue=5e-97,
Organism=Escherichia coli, GI1787096, Length=565, Percent_Identity=28.6725663716814, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=26.0714285714286, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=566, Percent_Identity=25.6183745583039, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=566, Percent_Identity=25.6183745583039, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=593, Percent_Identity=26.3069139966273, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6323755, Length=629, Percent_Identity=42.2893481717011, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=23.5655737704918, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6323163, Length=500, Percent_Identity=24, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=499, Percent_Identity=24.4488977955912, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=499, Percent_Identity=21.6432865731463, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=560, Percent_Identity=26.7857142857143, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65491; Mature: 65360

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPAKPKPASASGRAPGTAPEPNTVAPQRVTGAQSVVRSLEELGVEVIFGIPGGAVLPV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCEEEEEECCCCCEEEC
YDPLYDSTKVRHVLVRHEQGAGHAASGYAHANGKVGVMMATSGPGATNLVTPLADAQMDS
CCCCCCCHHHEEEEEEECCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
IPVVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLVSNAEDIPKVIAEAFYIAQSGR
CCEEEEECCCCCCCCCCCCHHHCCCCCEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCC
PGAVLVDIPKDILQAQTTFSWPPQIYLPGYKPVTKPHGKQVREAVRLIAAAKRPVLYVGG
CCEEEEECCHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
GVIRADASPELKELAELTGIPVVTTLMARGAFPDSHELHMGMPGMHGTVGAVAALQRSDL
EEEEECCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHCCCE
LITLGARFDDRVTGQLDSFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKNVIIELIEAI
EEEECCCCCCCCCCCHHHCCCCCEEEEECCCHHHCCCCCCCCCCEECCHHHHHHHHHHHH
RADRAAGTVADPLSEWWEYLSDVRKTYPLGYDAQHDGSLGPEFVIETLGKIAGPDAVYVA
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
GVGQHQMWAAQFIKYENPRTWLNSGGLGTMGFAVPAAMGAKMARPEAEVWAIDGDGCFQM
ECCCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
TNQELATCAIEGVPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSRRIPDFVKLAE
CCHHHHHEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHH
ALGCVGLRCEREEDVAKVIEEARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARG
HHCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHC
IRPLFDDDELVADE
CCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPAKPKPASASGRAPGTAPEPNTVAPQRVTGAQSVVRSLEELGVEVIFGIPGGAVLPV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHCCEEEEEECCCCCEEEC
YDPLYDSTKVRHVLVRHEQGAGHAASGYAHANGKVGVMMATSGPGATNLVTPLADAQMDS
CCCCCCCHHHEEEEEEECCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
IPVVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLVSNAEDIPKVIAEAFYIAQSGR
CCEEEEECCCCCCCCCCCCHHHCCCCCEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCC
PGAVLVDIPKDILQAQTTFSWPPQIYLPGYKPVTKPHGKQVREAVRLIAAAKRPVLYVGG
CCEEEEECCHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECC
GVIRADASPELKELAELTGIPVVTTLMARGAFPDSHELHMGMPGMHGTVGAVAALQRSDL
EEEEECCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCCCCHHHHHHHHHCCCE
LITLGARFDDRVTGQLDSFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKNVIIELIEAI
EEEECCCCCCCCCCCHHHCCCCCEEEEECCCHHHCCCCCCCCCCEECCHHHHHHHHHHHH
RADRAAGTVADPLSEWWEYLSDVRKTYPLGYDAQHDGSLGPEFVIETLGKIAGPDAVYVA
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEE
GVGQHQMWAAQFIKYENPRTWLNSGGLGTMGFAVPAAMGAKMARPEAEVWAIDGDGCFQM
ECCCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEE
TNQELATCAIEGVPIKVALINNGNLGMVRQWQTLFYEERYSQTDLATHSRRIPDFVKLAE
CCHHHHHEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHH
ALGCVGLRCEREEDVAKVIEEARAINDRPVVIDFIVGADAQVWPMVAAGTSNDEIQAARG
HHCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHC
IRPLFDDDELVADE
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]