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The map label for this gene is cysA [H]

Identifier: 169630368

GI number: 169630368

Start: 3325280

End: 3326392

Strand: Reverse

Name: cysA [H]

Synonym: MAB_3287c

Alternate gene names: 169630368

Gene position: 3326392-3325280 (Counterclockwise)

Preceding gene: 169630369

Following gene: 169630367

Centisome position: 65.65

GC content: 67.12

Gene sequence:

>1113_bases
ATGCCAGAGCCAGGGATATCGACCCGGGCCGTAGTGGCCGCCACTGCCGAACCCGTTGCCGGACAACCCTTTCTCGCGGG
ACCGGTGTTCGCCTCCGCCTACCACCTGCCGGTGCCCGAGGATGACTCCCTGGATACCTACGGGCGTGCCTCCAATCCCA
GTTGGAGGCAATGGGAATCGGGTCTGGCGGCGCTGGAGCAGGCCGATGCGGCACTGGTGTTCGGTTCGGGTATGGGCGCG
ATCGCCTCGACCTTGCGCGCGCTAGTCCGTCCCGGGCAGACCCTGTTGGTACCGGAGGACGGCTACTACCAAACGCGGCG
CTATGCCTCGGAAAACCTTGCCGCATTGGGAATCAACGTGATCACGGCGACGTCGGATCAGATAGTGGCCGCGGCCGCAC
AGGCCGATGTGGTGGTGGCGGAGACGCCGTCCAACCCACGGCTTGACGTCGTCGACCTGGCACTGCTGGCCCGTGAGACA
CACGCGCGGGGCGGAGTGCTGGTTGTCGACAACACCACGGCCACCCCGATGGGACAGCGGCCCCTGGGTTTGGGTGCCGA
CCTGGTGGTCGCCAGCGCGACAAAGGCGCTGTCCGGACACAGCGATCTGATCGCCGGATACGTCGCAGGTTCCCGCGCGG
AATTGGTTGCCGCCGTCGCCCAGGAACGGTCGCGGGCCGGTGCGATCCTGGGTCCGTTCGAGGCCTGGCTCGGTGCCCGC
AGCCTCGCGACTGCCGGACTGCGATTCGAGCGCCAATGCCAGAACGCGCTCGCGGTGGCGACCATGCTGCGTGAACATCC
CGCCGTGACGTCGGTGCGTTATCCGGGCCTGCCCAGCGACCCGTCGTATTCCGTGGCTTCCGCCCAGATGAGCCGGTTCG
GCTCGCTGGTGTCGATTGAACTGTCCAGTGCCGAGGCGGTACACGCGCTGACACGGCGTAGCGAGTTGTTGGCGTCGTCG
ACGAGTTTTGGCGGTGTGCATACCTCGGTCGACCGCCGGGCGCGGTGGGGAGACGCAGTTCCGGACGGTTTCGCACGCAT
CTCGCTGGGCATCGAGGACACCGACGATCTGCTGGCCGACATTGAGGCTGCGCTCGCAGCCGGTACGGTCTGA

Upstream 100 bases:

>100_bases
CCGCTCACCGAGGCTGTTCATGGCGTTTGTCATCGCGGACTGAGCGTCACCGATGCCGTCGCCCAGCTGTTGGGGCGCAG
TACCAAACCGGAGTAAGCAT

Downstream 100 bases:

>100_bases
CACTGTGAATGACTCGGTGGGCCGCTCGCGCGCAGACCAACAGCGGATCAAGGTCGCGCTCGTCTTTGGCGGGCGCAGCA
GTGAGCACGCGATCTCGTGC

Product: cystathionine gamma-lyase

Products: NA

Alternate protein names: Gamma-cystathionase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MPEPGISTRAVVAATAEPVAGQPFLAGPVFASAYHLPVPEDDSLDTYGRASNPSWRQWESGLAALEQADAALVFGSGMGA
IASTLRALVRPGQTLLVPEDGYYQTRRYASENLAALGINVITATSDQIVAAAAQADVVVAETPSNPRLDVVDLALLARET
HARGGVLVVDNTTATPMGQRPLGLGADLVVASATKALSGHSDLIAGYVAGSRAELVAAVAQERSRAGAILGPFEAWLGAR
SLATAGLRFERQCQNALAVATMLREHPAVTSVRYPGLPSDPSYSVASAQMSRFGSLVSIELSSAEAVHALTRRSELLASS
TSFGGVHTSVDRRARWGDAVPDGFARISLGIEDTDDLLADIEAALAAGTV

Sequences:

>Translated_370_residues
MPEPGISTRAVVAATAEPVAGQPFLAGPVFASAYHLPVPEDDSLDTYGRASNPSWRQWESGLAALEQADAALVFGSGMGA
IASTLRALVRPGQTLLVPEDGYYQTRRYASENLAALGINVITATSDQIVAAAAQADVVVAETPSNPRLDVVDLALLARET
HARGGVLVVDNTTATPMGQRPLGLGADLVVASATKALSGHSDLIAGYVAGSRAELVAAVAQERSRAGAILGPFEAWLGAR
SLATAGLRFERQCQNALAVATMLREHPAVTSVRYPGLPSDPSYSVASAQMSRFGSLVSIELSSAEAVHALTRRSELLASS
TSFGGVHTSVDRRARWGDAVPDGFARISLGIEDTDDLLADIEAALAAGTV
>Mature_369_residues
PEPGISTRAVVAATAEPVAGQPFLAGPVFASAYHLPVPEDDSLDTYGRASNPSWRQWESGLAALEQADAALVFGSGMGAI
ASTLRALVRPGQTLLVPEDGYYQTRRYASENLAALGINVITATSDQIVAAAAQADVVVAETPSNPRLDVVDLALLARETH
ARGGVLVVDNTTATPMGQRPLGLGADLVVASATKALSGHSDLIAGYVAGSRAELVAAVAQERSRAGAILGPFEAWLGARS
LATAGLRFERQCQNALAVATMLREHPAVTSVRYPGLPSDPSYSVASAQMSRFGSLVSIELSSAEAVHALTRRSELLASST
SFGGVHTSVDRRARWGDAVPDGFARISLGIEDTDDLLADIEAALAAGTV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=321, Percent_Identity=31.1526479750779, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI299473759, Length=265, Percent_Identity=30.9433962264151, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI25453487, Length=316, Percent_Identity=26.5822784810127, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790375, Length=358, Percent_Identity=32.9608938547486, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789383, Length=341, Percent_Identity=25.2199413489736, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17538055, Length=343, Percent_Identity=32.6530612244898, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71980708, Length=382, Percent_Identity=27.2251308900524, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17560056, Length=326, Percent_Identity=33.1288343558282, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319307, Length=348, Percent_Identity=35.0574712643678, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321254, Length=380, Percent_Identity=27.6315789473684, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=341, Percent_Identity=26.6862170087977, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6323333, Length=380, Percent_Identity=25.5263157894737, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321170, Length=288, Percent_Identity=25.6944444444444, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922554, Length=338, Percent_Identity=32.5443786982249, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.1 [H]

Molecular weight: Translated: 38352; Mature: 38221

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEPGISTRAVVAATAEPVAGQPFLAGPVFASAYHLPVPEDDSLDTYGRASNPSWRQWES
CCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCEEECCCCCCCCCCCCCCCCCCCHHHHHH
GLAALEQADAALVFGSGMGAIASTLRALVRPGQTLLVPEDGYYQTRRYASENLAALGINV
HHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCEEEEEEE
ITATSDQIVAAAAQADVVVAETPSNPRLDVVDLALLARETHARGGVLVVDNTTATPMGQR
EEECCCHHEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
PLGLGADLVVASATKALSGHSDLIAGYVAGSRAELVAAVAQERSRAGAILGPFEAWLGAR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHH
SLATAGLRFERQCQNALAVATMLREHPAVTSVRYPGLPSDPSYSVASAQMSRFGSLVSIE
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCEEEEE
LSSAEAVHALTRRSELLASSTSFGGVHTSVDRRARWGDAVPDGFARISLGIEDTDDLLAD
ECCHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHCCCCCCCCCEEEEECCCCHHHHHHH
IEAALAAGTV
HHHHHHCCCC
>Mature Secondary Structure 
PEPGISTRAVVAATAEPVAGQPFLAGPVFASAYHLPVPEDDSLDTYGRASNPSWRQWES
CCCCCCCCEEEEECCCCCCCCCCCCCCHHHCEEECCCCCCCCCCCCCCCCCCCHHHHHH
GLAALEQADAALVFGSGMGAIASTLRALVRPGQTLLVPEDGYYQTRRYASENLAALGINV
HHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCEEEEEEE
ITATSDQIVAAAAQADVVVAETPSNPRLDVVDLALLARETHARGGVLVVDNTTATPMGQR
EEECCCHHEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
PLGLGADLVVASATKALSGHSDLIAGYVAGSRAELVAAVAQERSRAGAILGPFEAWLGAR
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECHHHHHHHHH
SLATAGLRFERQCQNALAVATMLREHPAVTSVRYPGLPSDPSYSVASAQMSRFGSLVSIE
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCEEEEE
LSSAEAVHALTRRSELLASSTSFGGVHTSVDRRARWGDAVPDGFARISLGIEDTDDLLAD
ECCHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHCCCCCCCCCEEEEECCCCHHHHHHH
IEAALAAGTV
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953; 8550407 [H]