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The map label for this gene is ddl [H]

Identifier: 169630367

GI number: 169630367

Start: 3324130

End: 3325275

Strand: Reverse

Name: ddl [H]

Synonym: MAB_3286c

Alternate gene names: 169630367

Gene position: 3325275-3324130 (Counterclockwise)

Preceding gene: 169630368

Following gene: 169630365

Centisome position: 65.62

GC content: 66.93

Gene sequence:

>1146_bases
GTGAATGACTCGGTGGGCCGCTCGCGCGCAGACCAACAGCGGATCAAGGTCGCGCTCGTCTTTGGCGGGCGCAGCAGTGA
GCACGCGATCTCGTGCGTGTCCGCCGGCAGCATTCTGCGCAATCTGGACCAGGCCGTCTATGAGGTAGTCGCCGTGGGGG
TTACTCCCGAGGGCGGTTGGGTGCTGACCGATGGCGACCCCGCGCAGCTGGCGATCAGCGACAGGACGCTGCCTCAGGTC
ACCGACGCATCCGGCACCGAGTTGTTGCTCGCCACCGATCCGCGGCGAGCGGGGCAGCTGGTGAACGTGGACTCGAGCGC
CGCCGGGCAGGTGCTGGCCTCGGTCGATGTGGTGTTCCCGGTGCTGCACGGTCCCTATGGCGAGGATGGCACCATTCAGG
GACTGTTGGAGCTGGCCGGGGTGCCCTACGTGGGTGCCGGTGTGCTCGCGAGTGCGGCCGGTATGGACAAGGAGTTCACC
AAGAAGCTGCTGGCGGCCGAGGGGCTCCCGATTGGCAAGTACGCCGTGATCCGCCCGCGCGACACCACGCTGACGCTGGA
TCAGCGCGAGGACCTGGGGCTGCCGGTATTCGTCAAGCCCGCTCGGGCGGGATCGTCGTTCGGTGTGTCCCGGGTGACCG
CCTGGGAGGAACTACCTGCCGCGATCGCACACGCGCGTCAGTACGACCCGAAGGTGATTGTCGAGGCGGCGATCGTCGGC
CGCGAACTGGAGTGCGGAGTGCTGGAGTTCCCCGACGGGGATACTCGGGCCAGCGTGCTCGGCGAAATCCGGGTCGAGGG
AGTGAATAGGGACCAGACGGCTGCGGGTGCCTTCTACGACTTCGAGACCAAGTACCTGGACGACACCGCCGAATTGGACG
TACCGGCCAAGGTCGACGACGACGTGAGCGATCAGATCCGCGAACTTGCCGTGGCCGCGTTCCGGGCGATCGACTGCCAG
GGGCTGGCCCGGGTCGATTTCTTCCTCGCCGAGGATGGCCCGCTGATCAATGAGATCAACACCATGCCGGGGTTCACCAC
CATCTCCATGTACCCGCGGATGTGGGATGCGACCGGCGTGGACTATCCGACGCTGCTGGACGCGATGGTGCGCACCGCGC
TGGCGCGGGGTACCGGCCTTCGCTGA

Upstream 100 bases:

>100_bases
GTTCCGGACGGTTTCGCACGCATCTCGCTGGGCATCGAGGACACCGACGATCTGCTGGCCGACATTGAGGCTGCGCTCGC
AGCCGGTACGGTCTGACACT

Downstream 100 bases:

>100_bases
TTCACCTGGTGAGCATGCTCAAAAGTGCGTGAAATGGTCCCATTTCCGCACATTTGAGCATGCTCGCGGTGGAGGGTGCG
TCGCGTTGACTCAGCGGGGT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNDSVGRSRADQQRIKVALVFGGRSSEHAISCVSAGSILRNLDQAVYEVVAVGVTPEGGWVLTDGDPAQLAISDRTLPQV
TDASGTELLLATDPRRAGQLVNVDSSAAGQVLASVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAAGMDKEFT
KKLLAAEGLPIGKYAVIRPRDTTLTLDQREDLGLPVFVKPARAGSSFGVSRVTAWEELPAAIAHARQYDPKVIVEAAIVG
RELECGVLEFPDGDTRASVLGEIRVEGVNRDQTAAGAFYDFETKYLDDTAELDVPAKVDDDVSDQIRELAVAAFRAIDCQ
GLARVDFFLAEDGPLINEINTMPGFTTISMYPRMWDATGVDYPTLLDAMVRTALARGTGLR

Sequences:

>Translated_381_residues
MNDSVGRSRADQQRIKVALVFGGRSSEHAISCVSAGSILRNLDQAVYEVVAVGVTPEGGWVLTDGDPAQLAISDRTLPQV
TDASGTELLLATDPRRAGQLVNVDSSAAGQVLASVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAAGMDKEFT
KKLLAAEGLPIGKYAVIRPRDTTLTLDQREDLGLPVFVKPARAGSSFGVSRVTAWEELPAAIAHARQYDPKVIVEAAIVG
RELECGVLEFPDGDTRASVLGEIRVEGVNRDQTAAGAFYDFETKYLDDTAELDVPAKVDDDVSDQIRELAVAAFRAIDCQ
GLARVDFFLAEDGPLINEINTMPGFTTISMYPRMWDATGVDYPTLLDAMVRTALARGTGLR
>Mature_381_residues
MNDSVGRSRADQQRIKVALVFGGRSSEHAISCVSAGSILRNLDQAVYEVVAVGVTPEGGWVLTDGDPAQLAISDRTLPQV
TDASGTELLLATDPRRAGQLVNVDSSAAGQVLASVDVVFPVLHGPYGEDGTIQGLLELAGVPYVGAGVLASAAGMDKEFT
KKLLAAEGLPIGKYAVIRPRDTTLTLDQREDLGLPVFVKPARAGSSFGVSRVTAWEELPAAIAHARQYDPKVIVEAAIVG
RELECGVLEFPDGDTRASVLGEIRVEGVNRDQTAAGAFYDFETKYLDDTAELDVPAKVDDDVSDQIRELAVAAFRAIDCQ
GLARVDFFLAEDGPLINEINTMPGFTTISMYPRMWDATGVDYPTLLDAMVRTALARGTGLR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=377, Percent_Identity=41.6445623342175, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1786280, Length=255, Percent_Identity=36.078431372549, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40549; Mature: 40549

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSVGRSRADQQRIKVALVFGGRSSEHAISCVSAGSILRNLDQAVYEVVAVGVTPEGGW
CCCCCCCCCCCCCEEEEEEEECCCCCCHHEEHHHHHHHHHHHHHHHHHHEEEEECCCCCE
VLTDGDPAQLAISDRTLPQVTDASGTELLLATDPRRAGQLVNVDSSAAGQVLASVDVVFP
EEECCCCCEEEECCCCCCCCCCCCCCEEEEEECCHHCCCEEECCCHHHHHHHHHHHHHHH
VLHGPYGEDGTIQGLLELAGVPYVGAGVLASAAGMDKEFTKKLLAAEGLPIGKYAVIRPR
HHCCCCCCCCHHHHHHHHCCCCEECHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCC
DTTLTLDQREDLGLPVFVKPARAGSSFGVSRVTAWEELPAAIAHARQYDPKVIVEAAIVG
CCEEEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHC
RELECGVLEFPDGDTRASVLGEIRVEGVNRDQTAAGAFYDFETKYLDDTAELDVPAKVDD
CCCCCCEEECCCCCCHHHHEEEEEEECCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCC
DVSDQIRELAVAAFRAIDCQGLARVDFFLAEDGPLINEINTMPGFTTISMYPRMWDATGV
CHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHCCCCCEEEEEECCCCCCCCCC
DYPTLLDAMVRTALARGTGLR
CHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDSVGRSRADQQRIKVALVFGGRSSEHAISCVSAGSILRNLDQAVYEVVAVGVTPEGGW
CCCCCCCCCCCCCEEEEEEEECCCCCCHHEEHHHHHHHHHHHHHHHHHHEEEEECCCCCE
VLTDGDPAQLAISDRTLPQVTDASGTELLLATDPRRAGQLVNVDSSAAGQVLASVDVVFP
EEECCCCCEEEECCCCCCCCCCCCCCEEEEEECCHHCCCEEECCCHHHHHHHHHHHHHHH
VLHGPYGEDGTIQGLLELAGVPYVGAGVLASAAGMDKEFTKKLLAAEGLPIGKYAVIRPR
HHCCCCCCCCHHHHHHHHCCCCEECHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCC
DTTLTLDQREDLGLPVFVKPARAGSSFGVSRVTAWEELPAAIAHARQYDPKVIVEAAIVG
CCEEEECCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHC
RELECGVLEFPDGDTRASVLGEIRVEGVNRDQTAAGAFYDFETKYLDDTAELDVPAKVDD
CCCCCCEEECCCCCCHHHHEEEEEEECCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCC
DVSDQIRELAVAAFRAIDCQGLARVDFFLAEDGPLINEINTMPGFTTISMYPRMWDATGV
CHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHCCCCCEEEEEECCCCCCCCCC
DYPTLLDAMVRTALARGTGLR
CHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA