Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 169630315

GI number: 169630315

Start: 3269032

End: 3269895

Strand: Direct

Name: dacB [H]

Synonym: MAB_3234

Alternate gene names: 169630315

Gene position: 3269032-3269895 (Clockwise)

Preceding gene: 169630314

Following gene: 169630323

Centisome position: 64.51

GC content: 67.71

Gene sequence:

>864_bases
GTGTCGGATCCGATGTCCACTCTGCGAATCCGTGCCCTGGCGGTGCTCAGCGTCCTGTTCTTGTCCACACTCCCTGTCGG
CGTCGCCCGTGCGGACATGGCTGCCCCGCCGCCCGAGGGACCCGCGCAGGCCTGGTTGGTGGCAGATCTGGACTCGGGCC
AGATCCTGGCCGCCCGCGACCCCTACGCGACCCACGCCCCCGCCAGCACCATCAAGGTGCTGCTGGCACTGGTCGCGCTC
GATGAGGTGCCGATGGACGCCACGGTGGTGGCCGACGCCGCGGACGCGAAGGCCGAATGTAACTGTGTAGGCATCAAGGC
GGGACAGACCTACACCGCGCGGGAGCTGCTGGACGCCGCGCTGTTGGAGTCCGGCAACGACGCGGCCAACACCCTGGCCC
ATCTGCTCGGCGGGCGGCAAGCCACCGTCGACAAGATGAACGCCAAGGCCGCTGCCTTGGGCGCCACCTCCACGCACACC
GATTCGCCGTCCGGCCTGGACGCACCCGGCATGGACATGCGCACCAGCCCTCACGACTTGGCGGTCATCTTCCGGGCCGC
GCTGGCCAATCCGGTGTTCGCCGAGATCACCGCCCAGCCGAGCGCGCCGTTCCCCGGGCGCGGATTGCACAATCAGAATG
AGCTGATGTACCGCTATCCCGGCGTGATCGGCGGCAAGACGGGCTTCACCGATATCGCCCGCAAGACATATGTCGTGGCC
GCCGAGCGCGACGGCAAGCGCCTGGTGGTGTCGATGATGTACGGCCTGGTACATGAGGGGGGCCCGACGTACTGGGATCA
GGCCGCCAGCCTGTTCGACTGGGGCTTTGTCAATGACGGTTCGAGCAGTGTCGGTTCGCTCTAG

Upstream 100 bases:

>100_bases
CGCTGCAAGCTATTCCACATGATCGACTGCGAGAGCTGTATCAACAGCGGCAGTTCGTGGAATCGCTGGACCTGGATCAT
GTGGAAACCGCCCTGCGCTA

Downstream 100 bases:

>100_bases
CTCTTTGAGTTAAGCACCTGGAGCACGGTTTCGCCGGTGCGGACCCGGCACATCGCCTCGGCCAGCGCCACGGCGTCCCT
GGGCGAGATCTGTGCCACCG

Product: D-alanyl-D-alanine carboxypeptidase DacB

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSDPMSTLRIRALAVLSVLFLSTLPVGVARADMAAPPPEGPAQAWLVADLDSGQILAARDPYATHAPASTIKVLLALVAL
DEVPMDATVVADAADAKAECNCVGIKAGQTYTARELLDAALLESGNDAANTLAHLLGGRQATVDKMNAKAAALGATSTHT
DSPSGLDAPGMDMRTSPHDLAVIFRAALANPVFAEITAQPSAPFPGRGLHNQNELMYRYPGVIGGKTGFTDIARKTYVVA
AERDGKRLVVSMMYGLVHEGGPTYWDQAASLFDWGFVNDGSSSVGSL

Sequences:

>Translated_287_residues
MSDPMSTLRIRALAVLSVLFLSTLPVGVARADMAAPPPEGPAQAWLVADLDSGQILAARDPYATHAPASTIKVLLALVAL
DEVPMDATVVADAADAKAECNCVGIKAGQTYTARELLDAALLESGNDAANTLAHLLGGRQATVDKMNAKAAALGATSTHT
DSPSGLDAPGMDMRTSPHDLAVIFRAALANPVFAEITAQPSAPFPGRGLHNQNELMYRYPGVIGGKTGFTDIARKTYVVA
AERDGKRLVVSMMYGLVHEGGPTYWDQAASLFDWGFVNDGSSSVGSL
>Mature_286_residues
SDPMSTLRIRALAVLSVLFLSTLPVGVARADMAAPPPEGPAQAWLVADLDSGQILAARDPYATHAPASTIKVLLALVALD
EVPMDATVVADAADAKAECNCVGIKAGQTYTARELLDAALLESGNDAANTLAHLLGGRQATVDKMNAKAAALGATSTHTD
SPSGLDAPGMDMRTSPHDLAVIFRAALANPVFAEITAQPSAPFPGRGLHNQNELMYRYPGVIGGKTGFTDIARKTYVVAA
ERDGKRLVVSMMYGLVHEGGPTYWDQAASLFDWGFVNDGSSSVGSL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=284, Percent_Identity=32.0422535211268, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786851, Length=253, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082054, Length=250, Percent_Identity=26.4, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787062, Length=282, Percent_Identity=28.0141843971631, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 29932; Mature: 29801

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPMSTLRIRALAVLSVLFLSTLPVGVARADMAAPPPEGPAQAWLVADLDSGQILAARD
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEEEECC
PYATHAPASTIKVLLALVALDEVPMDATVVADAADAKAECNCVGIKAGQTYTARELLDAA
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHH
LLESGNDAANTLAHLLGGRQATVDKMNAKAAALGATSTHTDSPSGLDAPGMDMRTSPHDL
HHHCCCHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AVIFRAALANPVFAEITAQPSAPFPGRGLHNQNELMYRYPGVIGGKTGFTDIARKTYVVA
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCEECCCCCHHHHHHEEEEEE
AERDGKRLVVSMMYGLVHEGGPTYWDQAASLFDWGFVNDGSSSVGSL
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
SDPMSTLRIRALAVLSVLFLSTLPVGVARADMAAPPPEGPAQAWLVADLDSGQILAARD
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEEEECC
PYATHAPASTIKVLLALVALDEVPMDATVVADAADAKAECNCVGIKAGQTYTARELLDAA
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHH
LLESGNDAANTLAHLLGGRQATVDKMNAKAAALGATSTHTDSPSGLDAPGMDMRTSPHDL
HHHCCCHHHHHHHHHHCCCHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AVIFRAALANPVFAEITAQPSAPFPGRGLHNQNELMYRYPGVIGGKTGFTDIARKTYVVA
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCEECCCCCHHHHHHEEEEEE
AERDGKRLVVSMMYGLVHEGGPTYWDQAASLFDWGFVNDGSSSVGSL
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]