BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is paaF [H]

Identifier: 169630206

GI number: 169630206

Start: 3164897

End: 3165655

Strand: Direct

Name: paaF [H]

Synonym: MAB_3124

Alternate gene names: 169630206

Gene position: 3164897-3165655 (Clockwise)

Preceding gene: 169630205

Following gene: 169630209

Centisome position: 62.46

GC content: 67.85

Gene sequence:

>759_bases
ATGACCGAACCAATTTTGTTGACCCACACCGAAGACCGCATCTGCACCATCACCCTGAACCGGCCGCAAGCACGTAACGC
CTTGAGCACCGCCCTGGGCGAGGAGATCGTCAAGGCCGTCACCGCGGCCGACGCCGACGAGAACGTCGACGTGATGATCC
TGACCGCGGCCGACCCGGTCTTCTGCGCGGGCGTGGACCTCAAGGAGCTGGGCAGTGGCGATCGTGCCGAGTCCCTGGAC
CCGTGGTGGCCCGAACTGAGCAAGCCGGTCATCGGCGCCATCAACGGCGCTGCGGTGACCGGCGGTCTGGAGTTGGCACT
GGCCTGCGACATTCTCATCGCCTCGGAGAAGGCACGATTCGCCGACACCCATGCGCGCGTTGGCATCTTGCCGACCTGGG
GGCTGACCACCCTGCTGCCGCTCTCGGTGGGGCGGGGCCTGGCCCGGCGGATGAGCCTGACCGGCGACTACCTCTCCGCC
GAGGATGCGCTGCGGGCAGGCCTGGTGACCGAGGTGGTCGCGCACAACGAGCTCCTCCCCGCCGCCAAGCGTCTCGCCGC
GACCATTGCCGGCAACAACCAGCCCGCGGTCCGCGAACTGCTGGCGTCCTACCGCCGTATAGAGTCCGAGCTCGTCGGCA
ACGGCCTGCAGGTGGCTCTCGACGATGCTCACCGGTGGATCGATCAGAACTCGATCGCCGAGGGCGTAGAGGCTCGGCGC
ACCGCGATCATGGAGCGCGGACGCAGCCAGAACGCCTAG

Upstream 100 bases:

>100_bases
ACCCGGCCCATCTGTATTACAAACGCGCCGTGAGTAACTCGGCCCTCTTCGGGGGGCGCGCCGTCCACGCCGAACGTATT
GCCAAGGAGGTAATCGACGC

Downstream 100 bases:

>100_bases
GCGCGATCGGTTCGGTACAACGCGATCCGGCGATCGGGCCGAATATCGAACTCACCCAGGAACGTCCAGCCAATCCGGTC
GAGCGCCTTGCGCATCGGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTEPILLTHTEDRICTITLNRPQARNALSTALGEEIVKAVTAADADENVDVMILTAADPVFCAGVDLKELGSGDRAESLD
PWWPELSKPVIGAINGAAVTGGLELALACDILIASEKARFADTHARVGILPTWGLTTLLPLSVGRGLARRMSLTGDYLSA
EDALRAGLVTEVVAHNELLPAAKRLAATIAGNNQPAVRELLASYRRIESELVGNGLQVALDDAHRWIDQNSIAEGVEARR
TAIMERGRSQNA

Sequences:

>Translated_252_residues
MTEPILLTHTEDRICTITLNRPQARNALSTALGEEIVKAVTAADADENVDVMILTAADPVFCAGVDLKELGSGDRAESLD
PWWPELSKPVIGAINGAAVTGGLELALACDILIASEKARFADTHARVGILPTWGLTTLLPLSVGRGLARRMSLTGDYLSA
EDALRAGLVTEVVAHNELLPAAKRLAATIAGNNQPAVRELLASYRRIESELVGNGLQVALDDAHRWIDQNSIAEGVEARR
TAIMERGRSQNA
>Mature_251_residues
TEPILLTHTEDRICTITLNRPQARNALSTALGEEIVKAVTAADADENVDVMILTAADPVFCAGVDLKELGSGDRAESLDP
WWPELSKPVIGAINGAAVTGGLELALACDILIASEKARFADTHARVGILPTWGLTTLLPLSVGRGLARRMSLTGDYLSAE
DALRAGLVTEVVAHNELLPAAKRLAATIAGNNQPAVRELLASYRRIESELVGNGLQVALDDAHRWIDQNSIAEGVEARRT
AIMERGRSQNA

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=188, Percent_Identity=40.4255319148936, Blast_Score=127, Evalue=6e-30,
Organism=Homo sapiens, GI31542718, Length=213, Percent_Identity=34.7417840375587, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI70995211, Length=199, Percent_Identity=26.6331658291457, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI25453481, Length=182, Percent_Identity=29.6703296703297, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI51538573, Length=182, Percent_Identity=29.6703296703297, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI4757966, Length=182, Percent_Identity=29.6703296703297, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI51538581, Length=182, Percent_Identity=29.6703296703297, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI4502327, Length=197, Percent_Identity=34.5177664974619, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI37594469, Length=191, Percent_Identity=29.8429319371728, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI37594471, Length=191, Percent_Identity=29.8429319371728, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4757968, Length=182, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI49355787, Length=182, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI116235487, Length=198, Percent_Identity=30.3030303030303, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45643119, Length=181, Percent_Identity=28.7292817679558, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260275230, Length=181, Percent_Identity=28.7292817679558, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=181, Percent_Identity=28.7292817679558, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI221307494, Length=161, Percent_Identity=29.8136645962733, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221136756, Length=182, Percent_Identity=28.021978021978, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI221136753, Length=182, Percent_Identity=28.021978021978, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI20127408, Length=174, Percent_Identity=29.3103448275862, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI68989263, Length=175, Percent_Identity=29.7142857142857, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=192, Percent_Identity=39.5833333333333, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI221142681, Length=238, Percent_Identity=34.0336134453782, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787660, Length=206, Percent_Identity=33.0097087378641, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788597, Length=214, Percent_Identity=32.2429906542056, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788682, Length=214, Percent_Identity=32.7102803738318, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=27.7486910994764, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=187, Percent_Identity=34.2245989304813, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=238, Percent_Identity=33.6134453781513, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=33.8888888888889, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=29.8507462686567, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=200, Percent_Identity=29.5, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=190, Percent_Identity=30, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=221, Percent_Identity=28.9592760180996, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=221, Percent_Identity=28.9592760180996, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=214, Percent_Identity=28.9719626168224, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=190, Percent_Identity=28.4210526315789, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=213, Percent_Identity=27.6995305164319, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129971, Length=180, Percent_Identity=36.6666666666667, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653477, Length=180, Percent_Identity=36.6666666666667, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650670, Length=195, Percent_Identity=33.8461538461538, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653139, Length=188, Percent_Identity=35.1063829787234, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21357171, Length=203, Percent_Identity=30.0492610837438, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=24.0641711229947, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583165, Length=251, Percent_Identity=22.7091633466135, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922422, Length=185, Percent_Identity=26.4864864864865, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921018, Length=178, Percent_Identity=27.5280898876405, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 26860; Mature: 26729

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPILLTHTEDRICTITLNRPQARNALSTALGEEIVKAVTAADADENVDVMILTAADPV
CCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCE
FCAGVDLKELGSGDRAESLDPWWPELSKPVIGAINGAAVTGGLELALACDILIASEKARF
EECCCCHHHHCCCCCCCCCCCCCHHHCCCCEECCCCCEEECCHHHHHHHHHHEECCCHHH
ADTHARVGILPTWGLTTLLPLSVGRGLARRMSLTGDYLSAEDALRAGLVTEVVAHNELLP
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAKRLAATIAGNNQPAVRELLASYRRIESELVGNGLQVALDDAHRWIDQNSIAEGVEARR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHCHHHHHHHHHHHH
TAIMERGRSQNA
HHHHHCCCCCCC
>Mature Secondary Structure 
TEPILLTHTEDRICTITLNRPQARNALSTALGEEIVKAVTAADADENVDVMILTAADPV
CCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCE
FCAGVDLKELGSGDRAESLDPWWPELSKPVIGAINGAAVTGGLELALACDILIASEKARF
EECCCCHHHHCCCCCCCCCCCCCHHHCCCCEECCCCCEEECCHHHHHHHHHHEECCCHHH
ADTHARVGILPTWGLTTLLPLSVGRGLARRMSLTGDYLSAEDALRAGLVTEVVAHNELLP
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAKRLAATIAGNNQPAVRELLASYRRIESELVGNGLQVALDDAHRWIDQNSIAEGVEARR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHCHHHHHHHHHHHH
TAIMERGRSQNA
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]