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The map label for this gene is priA [H]

Identifier: 169629897

GI number: 169629897

Start: 2861242

End: 2863257

Strand: Reverse

Name: priA [H]

Synonym: MAB_2813c

Alternate gene names: 169629897

Gene position: 2863257-2861242 (Counterclockwise)

Preceding gene: 169629899

Following gene: 169629895

Centisome position: 56.51

GC content: 70.88

Gene sequence:

>2016_bases
GTGACTGCGACGCGGGTGGTGGCCGAACACGAGCCCATTGCTCGGGTGCTGCCGTTGCTCACCGTGCCGCATCTGGATCG
GGAGTTCGACTATCTCGTATCGGCCGAGCAATCGGACGATGCCCAGCCCGGTGTGCGGGTGCGGGTGCGTTTCCACGGCC
GCCTGGTGGACGCCATCCTGCTGGAGCGCCGTTCGGAAAGTGACCACTCTGGCAAGCTCGGCTGGCTGGATCGCGTCGTA
TCTCCTACCCGGGTGCTCACCCCGGATGTCCGCAGGCTCGTCGACGCGGTGGCGGCTCGCTACGCCGGGACTCGCGCGGA
TGTGCTGCGCCTCGCGCTGCCGCCGCGTCACGCACGCGTCGAAAAGGAAAACCGGGTGCCAGGCGCGGACGGCTTGCCGC
CGGAAACTCCGGATCGCTCCGGCTGGTCGCGTTATCAGCGCGGAGAACGATTCCTGGACGCGCTGACGCACGGCCGTGCC
GCGCGCGCGGTCTGGCAGGCGCTGCCGGGGGAGGCCTGGTGCCTGCGGCTTGCCGAGGCCGCGCGGGCCACCGCGAGCAC
CGGCAAGGGAGTGTTGGCGATTGTTCCCGACCAGCGGGATATCGACGCGCTGTCCGCTGAATGCGTGAAAAACTTTGGCG
TGCAACGTGTTGTCGCGCTCTCGGCCGAGTTGGGGCCGGCCGAACGATATCGGCGGTGGTTGGCGGTGCTACGCGGCCAA
GCGACTGTGGTGATCGGCACCAGGGCTGCGGTGTTCGCGCCGGTGGTCGATCTGGGACTGGTGATGGTGTGGGACGACGG
CGACGACAATCTTGCCGAGCCGCGTTCTCCCTATCCGCATGCTAGGGACGTGGCGATGCTGCGCGCCCACCAATTGCGCT
GCGCCGCGGTGATCGGGGGGTATGCCCGTACGGCCGAGTCGCAGGCGCTCGTCGAAGGCGGCTGGGCGCACGATCTGGTG
GCCGCCCGGCCCACGGTGCGGAAGTCCTCGGCACGGATCAGTGCGCTCGATGACAGCGGATACGCGCAGGAGCGTGATCC
GGCGGCACGTTCGGCGCGCCTGCCGATGGTCGCGTTGACCGCGGCCCGCGACGCACTGAAGCGCGGTCATTCAGTACTTG
TGCAGGTGCCGCGCCGTGGTTATGTGCCGGCGCTGGCCTGTACGAAATGCCGGACGGTGGCGCGGTGCCGTCACTGCACC
GGGCCGTTGGCATGGGAGGGGCCCGGCGGGGCATCGCACAGTGCGAGCCCCAGCTGTCGATGGTGCGGACGGGTAGACGC
CGACTTGCGTTGTGCGCGCTGCGGCTCCAGTGCGGTACGTGCTGTGGTGGTGGGCGCTCGCAGGACGGCCGAGGAGCTCG
GCAGAGCGCTGCCCGGGGTGCCGGTCGTGACCTCCGGTGGCGGTGAGGTCCATGACACGGTGACCGGGCCGCCGACCCTG
GTGGTGTGCACTCCCGGGGCGGAGCCGGTGACACCGGGCGGGTATGGCGCGGCGCTGCTGCTAGACGCGTGGGCACTGCT
GGGACGTCAGGATCTACGCGCCGCGGAGGACACCCTGCGCAAGTGGATGGCTGCCGCGGCCTTGGTGCGCGGGCGCGATG
AGGGCGGAACGGTGGTGGTGGTCGCGGATTCCTCCCTGTCCACCGTGCAAGCGCTCATTCGGTGGGATCCGGTGGGACAC
GCGGCCACCGAATTGGCGGCGCGCGCGGAGGTGGGGCTGCCGCCGCAGGTGCACATCGCCGCCGTCGACGGGCCCGCCCA
GGCGGTCGCCGGCCTCATTGACACGGCCGACCTGCCGGAGGGGGCCGAGCTGCTGGGGCCCGTCGAGCTGCCGCTTGGGG
CGCGGCGTCCGGCGGGTGTGCCCGACAGCGTCGAGGTGGTGCGCATACTGGTTCGGGTCGAACGCGCCGCCGGACTGGAA
CTCGCCGCGGCTTTGCGCCGCGGCGTCACCACGCTGAGCGCTCGCCGCGACGCCCAGCCCGTCAGGGTTCAGATCGATCC
GCTCCACGTGGGCTGA

Upstream 100 bases:

>100_bases
CGGGGACTTACCCGGGACGGGCCACACTGGGTAGCGCACATCGGCGACCGTGCGGTGGTGGATCCGCGGTTGCGTCGGCT
GCGTCTGCTAGAACGACCAG

Downstream 100 bases:

>100_bases
TCTGGCGAATTTCAGCAATTTGCGGCGACGAGCCGGGACCCCTCACCAATGCCTTCGGCGAAGGTCTCCACGATGTAGCC
GATGAGATCGTCTTGAGTCA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MTATRVVAEHEPIARVLPLLTVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDAILLERRSESDHSGKLGWLDRVV
SPTRVLTPDVRRLVDAVAARYAGTRADVLRLALPPRHARVEKENRVPGADGLPPETPDRSGWSRYQRGERFLDALTHGRA
ARAVWQALPGEAWCLRLAEAARATASTGKGVLAIVPDQRDIDALSAECVKNFGVQRVVALSAELGPAERYRRWLAVLRGQ
ATVVIGTRAAVFAPVVDLGLVMVWDDGDDNLAEPRSPYPHARDVAMLRAHQLRCAAVIGGYARTAESQALVEGGWAHDLV
AARPTVRKSSARISALDDSGYAQERDPAARSARLPMVALTAARDALKRGHSVLVQVPRRGYVPALACTKCRTVARCRHCT
GPLAWEGPGGASHSASPSCRWCGRVDADLRCARCGSSAVRAVVVGARRTAEELGRALPGVPVVTSGGGEVHDTVTGPPTL
VVCTPGAEPVTPGGYGAALLLDAWALLGRQDLRAAEDTLRKWMAAAALVRGRDEGGTVVVVADSSLSTVQALIRWDPVGH
AATELAARAEVGLPPQVHIAAVDGPAQAVAGLIDTADLPEGAELLGPVELPLGARRPAGVPDSVEVVRILVRVERAAGLE
LAAALRRGVTTLSARRDAQPVRVQIDPLHVG

Sequences:

>Translated_671_residues
MTATRVVAEHEPIARVLPLLTVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDAILLERRSESDHSGKLGWLDRVV
SPTRVLTPDVRRLVDAVAARYAGTRADVLRLALPPRHARVEKENRVPGADGLPPETPDRSGWSRYQRGERFLDALTHGRA
ARAVWQALPGEAWCLRLAEAARATASTGKGVLAIVPDQRDIDALSAECVKNFGVQRVVALSAELGPAERYRRWLAVLRGQ
ATVVIGTRAAVFAPVVDLGLVMVWDDGDDNLAEPRSPYPHARDVAMLRAHQLRCAAVIGGYARTAESQALVEGGWAHDLV
AARPTVRKSSARISALDDSGYAQERDPAARSARLPMVALTAARDALKRGHSVLVQVPRRGYVPALACTKCRTVARCRHCT
GPLAWEGPGGASHSASPSCRWCGRVDADLRCARCGSSAVRAVVVGARRTAEELGRALPGVPVVTSGGGEVHDTVTGPPTL
VVCTPGAEPVTPGGYGAALLLDAWALLGRQDLRAAEDTLRKWMAAAALVRGRDEGGTVVVVADSSLSTVQALIRWDPVGH
AATELAARAEVGLPPQVHIAAVDGPAQAVAGLIDTADLPEGAELLGPVELPLGARRPAGVPDSVEVVRILVRVERAAGLE
LAAALRRGVTTLSARRDAQPVRVQIDPLHVG
>Mature_670_residues
TATRVVAEHEPIARVLPLLTVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDAILLERRSESDHSGKLGWLDRVVS
PTRVLTPDVRRLVDAVAARYAGTRADVLRLALPPRHARVEKENRVPGADGLPPETPDRSGWSRYQRGERFLDALTHGRAA
RAVWQALPGEAWCLRLAEAARATASTGKGVLAIVPDQRDIDALSAECVKNFGVQRVVALSAELGPAERYRRWLAVLRGQA
TVVIGTRAAVFAPVVDLGLVMVWDDGDDNLAEPRSPYPHARDVAMLRAHQLRCAAVIGGYARTAESQALVEGGWAHDLVA
ARPTVRKSSARISALDDSGYAQERDPAARSARLPMVALTAARDALKRGHSVLVQVPRRGYVPALACTKCRTVARCRHCTG
PLAWEGPGGASHSASPSCRWCGRVDADLRCARCGSSAVRAVVVGARRTAEELGRALPGVPVVTSGGGEVHDTVTGPPTLV
VCTPGAEPVTPGGYGAALLLDAWALLGRQDLRAAEDTLRKWMAAAALVRGRDEGGTVVVVADSSLSTVQALIRWDPVGHA
ATELAARAEVGLPPQVHIAAVDGPAQAVAGLIDTADLPEGAELLGPVELPLGARRPAGVPDSVEVVRILVRVERAAGLEL
AAALRRGVTTLSARRDAQPVRVQIDPLHVG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=484, Percent_Identity=25.6198347107438, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 71416; Mature: 71285

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRVVAEHEPIARVLPLLTVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDAIL
CCCCCEECCCCCHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCCEEEEEECCHHHHHHH
LERRSESDHSGKLGWLDRVVSPTRVLTPDVRRLVDAVAARYAGTRADVLRLALPPRHARV
HHHCCCCCCCCCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHCCCHHHHHEECCCCCHHCC
EKENRVPGADGLPPETPDRSGWSRYQRGERFLDALTHGRAARAVWQALPGEAWCLRLAEA
CHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
ARATASTGKGVLAIVPDQRDIDALSAECVKNFGVQRVVALSAELGPAERYRRWLAVLRGQ
HHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCHHEEEHHHCCCCHHHHHHHHHHHHCCC
ATVVIGTRAAVFAPVVDLGLVMVWDDGDDNLAEPRSPYPHARDVAMLRAHQLRCAAVIGG
EEEEEECHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
YARTAESQALVEGGWAHDLVAARPTVRKSSARISALDDSGYAQERDPAARSARLPMVALT
HHHHCCCHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCHHHHH
AARDALKRGHSVLVQVPRRGYVPALACTKCRTVARCRHCTGPLAWEGPGGASHSASPSCR
HHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
WCGRVDADLRCARCGSSAVRAVVVGARRTAEELGRALPGVPVVTSGGGEVHDTVTGPPTL
CCCCCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEE
VVCTPGAEPVTPGGYGAALLLDAWALLGRQDLRAAEDTLRKWMAAAALVRGRDEGGTVVV
EEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
VADSSLSTVQALIRWDPVGHAATELAARAEVGLPPQVHIAAVDGPAQAVAGLIDTADLPE
EECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCC
GAELLGPVELPLGARRPAGVPDSVEVVRILVRVERAAGLELAAALRRGVTTLSARRDAQP
CHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCCC
VRVQIDPLHVG
EEEEEEEEECC
>Mature Secondary Structure 
TATRVVAEHEPIARVLPLLTVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDAIL
CCCCEECCCCCHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCCCEEEEEECCHHHHHHH
LERRSESDHSGKLGWLDRVVSPTRVLTPDVRRLVDAVAARYAGTRADVLRLALPPRHARV
HHHCCCCCCCCCCHHHHHHCCCCHHCCHHHHHHHHHHHHHHCCCHHHHHEECCCCCHHCC
EKENRVPGADGLPPETPDRSGWSRYQRGERFLDALTHGRAARAVWQALPGEAWCLRLAEA
CHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
ARATASTGKGVLAIVPDQRDIDALSAECVKNFGVQRVVALSAELGPAERYRRWLAVLRGQ
HHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCHHEEEHHHCCCCHHHHHHHHHHHHCCC
ATVVIGTRAAVFAPVVDLGLVMVWDDGDDNLAEPRSPYPHARDVAMLRAHQLRCAAVIGG
EEEEEECHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
YARTAESQALVEGGWAHDLVAARPTVRKSSARISALDDSGYAQERDPAARSARLPMVALT
HHHHCCCHHHHCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCHHHHH
AARDALKRGHSVLVQVPRRGYVPALACTKCRTVARCRHCTGPLAWEGPGGASHSASPSCR
HHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
WCGRVDADLRCARCGSSAVRAVVVGARRTAEELGRALPGVPVVTSGGGEVHDTVTGPPTL
CCCCCCCCCHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEE
VVCTPGAEPVTPGGYGAALLLDAWALLGRQDLRAAEDTLRKWMAAAALVRGRDEGGTVVV
EEECCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
VADSSLSTVQALIRWDPVGHAATELAARAEVGLPPQVHIAAVDGPAQAVAGLIDTADLPE
EECCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCC
GAELLGPVELPLGARRPAGVPDSVEVVRILVRVERAAGLELAAALRRGVTTLSARRDAQP
CHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCCC
VRVQIDPLHVG
EEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]