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The map label for this gene is pucI [H]

Identifier: 169629899

GI number: 169629899

Start: 2864295

End: 2865740

Strand: Reverse

Name: pucI [H]

Synonym: MAB_2815c

Alternate gene names: 169629899

Gene position: 2865740-2864295 (Counterclockwise)

Preceding gene: 169629900

Following gene: 169629897

Centisome position: 56.56

GC content: 64.87

Gene sequence:

>1446_bases
GTGGTCCTGGAGGCGGCCGGCCCGGCGGCCGGTAGATTGAGTAACGATGACCTGGCGCCCACCACGGCGGCACAGGCCAC
CTGGCGCACGTGGGACATCTTCTGTCTCTGGATGACCTCCGCGCACAGCCTGGGCAGCTACGGATTCGCGGTGGGCATGC
TGGCGCTGGGTCTTACCGGTTGGCAGACGATGCTGGCGCTCTTCACCGGGGTGCTGGTGCTCTGGACGGGGAGCAATCTG
ATCGGGATAGCCGGCCAACGGCTGGGCGTGCCCTTCCCGGTTTTCGCCCGCGCCACGTTCGGGGTGTTCGGCGCCAACAT
CCCGGCGCTGCTGCGCGGCCTGGTCGCTATTTTCTGGTACGGCATCCAGACCTACCTGGCATCCACGGCGGTTATCGTGC
TGCTGCTACGGCTGGACCCGGGACTGCGGTCCTGGACGGAACCGACATTCCTGGGCCTACATGCCTTGGGGTGGGCCGCA
TTCCTTTCCCTGTGGACTGTGCAGCTGCTGATCCTGAACTACGGCATGGAAGTGGTGCGCAAGGCATCGGATGTGGCCGG
CCCGGTGGTCTGGATCGGGATGCTCGCCCTGGCCGTATGGATGTTGGACAGTGTGGGCTGGAGCATCGACTTCGGCGCTC
GTACCGGCGCACCGTTGTCGGCTGGGGCCACGGTGTTGGCGTTCGTCGGGGTGGCTTTCCTGATGGTCGCTTTCATGGCG
GGTCCGCTGGTCAACTATGCCGACTTCGCGCGTTTCGCGCCGAGTAGGCGTGCGATTCTGCGGGGCAACGCACTGGGTAT
CCCGTTCAACGAGACGGCCTTCGTGGTGATCTCGATCGTCATTTCGCTTGCCGCACTGAAGAAGTACGGCAGTGCGATGA
CCGATCCGGTCGAGCTGGTCGCGGATATCGACTCGGATGCCGTCGTGGTGGTCGCCGCGGTTCTTTTCGCGATCGCCACC
GTCGGCATCAACGTGGTGCTCAACTTCGTCTCGCCGTCGTACGACATTTCCAATGTGGCACCGAAGTACATCTCATTTCG
CACCGGGGGCATCATCACCGCGGTGCTGTCGGTCGCGGTGCTGCCCTGGAAAATCTGGGGCAACGAGGCCGCGATCGTGT
ACTTCCTGGGTGGTGTGGGCGCTTTGATGGGACCGGTGTTCGGCATCCTGGTCGTGGACTACTACCTGCTGCGCCGGCAA
CACACCCACACCGCCGACCTGTATTCCGAATCCCCCGGAGGCGCGTATCACTACGCCGGCGGCTTCAATCCGGCCGCGCT
GATCTCGCTGGGTATCGGCGGCATTGTGGCGGTCGTTATCGCGCTGGTGCCGCGAGCCCAGGGCGTGACCGCCCTTTCCT
GGCCGGTCGGCGCCCTGCTGGGCGGCGTACTCATGTACCTGTTGGGGCGTGGCAGGTCCCGGTCTAGCCGTGTAGGGCCT
GCTTGA

Upstream 100 bases:

>100_bases
CCGGCTACCTCCAACACGACAACAAGGAAGAGACGCTGCGGGTCTATGGAGAGACAGTGATACCCGCACTGTCACAACAC
ATCACGGCGAAGAGCGGCTA

Downstream 100 bases:

>100_bases
GCGCGGCCAGGGAAAGGTCGCGCTCCTCGGTGGCGGACGGCACGATATCGGCGTAGCCGAGGTAACCGTGGATCAACGTC
GGCGCGTTGTGTAGCTGTAC

Product: NCS1 nucleoside transporter family protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MVLEAAGPAAGRLSNDDLAPTTAAQATWRTWDIFCLWMTSAHSLGSYGFAVGMLALGLTGWQTMLALFTGVLVLWTGSNL
IGIAGQRLGVPFPVFARATFGVFGANIPALLRGLVAIFWYGIQTYLASTAVIVLLLRLDPGLRSWTEPTFLGLHALGWAA
FLSLWTVQLLILNYGMEVVRKASDVAGPVVWIGMLALAVWMLDSVGWSIDFGARTGAPLSAGATVLAFVGVAFLMVAFMA
GPLVNYADFARFAPSRRAILRGNALGIPFNETAFVVISIVISLAALKKYGSAMTDPVELVADIDSDAVVVVAAVLFAIAT
VGINVVLNFVSPSYDISNVAPKYISFRTGGIITAVLSVAVLPWKIWGNEAAIVYFLGGVGALMGPVFGILVVDYYLLRRQ
HTHTADLYSESPGGAYHYAGGFNPAALISLGIGGIVAVVIALVPRAQGVTALSWPVGALLGGVLMYLLGRGRSRSSRVGP
A

Sequences:

>Translated_481_residues
MVLEAAGPAAGRLSNDDLAPTTAAQATWRTWDIFCLWMTSAHSLGSYGFAVGMLALGLTGWQTMLALFTGVLVLWTGSNL
IGIAGQRLGVPFPVFARATFGVFGANIPALLRGLVAIFWYGIQTYLASTAVIVLLLRLDPGLRSWTEPTFLGLHALGWAA
FLSLWTVQLLILNYGMEVVRKASDVAGPVVWIGMLALAVWMLDSVGWSIDFGARTGAPLSAGATVLAFVGVAFLMVAFMA
GPLVNYADFARFAPSRRAILRGNALGIPFNETAFVVISIVISLAALKKYGSAMTDPVELVADIDSDAVVVVAAVLFAIAT
VGINVVLNFVSPSYDISNVAPKYISFRTGGIITAVLSVAVLPWKIWGNEAAIVYFLGGVGALMGPVFGILVVDYYLLRRQ
HTHTADLYSESPGGAYHYAGGFNPAALISLGIGGIVAVVIALVPRAQGVTALSWPVGALLGGVLMYLLGRGRSRSSRVGP
A
>Mature_481_residues
MVLEAAGPAAGRLSNDDLAPTTAAQATWRTWDIFCLWMTSAHSLGSYGFAVGMLALGLTGWQTMLALFTGVLVLWTGSNL
IGIAGQRLGVPFPVFARATFGVFGANIPALLRGLVAIFWYGIQTYLASTAVIVLLLRLDPGLRSWTEPTFLGLHALGWAA
FLSLWTVQLLILNYGMEVVRKASDVAGPVVWIGMLALAVWMLDSVGWSIDFGARTGAPLSAGATVLAFVGVAFLMVAFMA
GPLVNYADFARFAPSRRAILRGNALGIPFNETAFVVISIVISLAALKKYGSAMTDPVELVADIDSDAVVVVAAVLFAIAT
VGINVVLNFVSPSYDISNVAPKYISFRTGGIITAVLSVAVLPWKIWGNEAAIVYFLGGVGALMGPVFGILVVDYYLLRRQ
HTHTADLYSESPGGAYHYAGGFNPAALISLGIGGIVAVVIALVPRAQGVTALSWPVGALLGGVLMYLLGRGRSRSSRVGP
A

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=471, Percent_Identity=29.9363057324841, Blast_Score=194, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6319495, Length=486, Percent_Identity=24.6913580246914, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322219, Length=482, Percent_Identity=22.6141078838174, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6319429, Length=477, Percent_Identity=22.8511530398323, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324766, Length=461, Percent_Identity=22.5596529284165, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323266, Length=471, Percent_Identity=23.1422505307856, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324645, Length=480, Percent_Identity=20.4166666666667, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 50655; Mature: 50655

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLEAAGPAAGRLSNDDLAPTTAAQATWRTWDIFCLWMTSAHSLGSYGFAVGMLALGLTG
CEECCCCCCCCCCCCCCCCCCHHHHCCCCHHEEEEEEEHHHHCCCHHHHHHHHHHHHHHH
WQTMLALFTGVLVLWTGSNLIGIAGQRLGVPFPVFARATFGVFGANIPALLRGLVAIFWY
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
GIQTYLASTAVIVLLLRLDPGLRSWTEPTFLGLHALGWAAFLSLWTVQLLILNYGMEVVR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KASDVAGPVVWIGMLALAVWMLDSVGWSIDFGARTGAPLSAGATVLAFVGVAFLMVAFMA
HHHHHCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GPLVNYADFARFAPSRRAILRGNALGIPFNETAFVVISIVISLAALKKYGSAMTDPVELV
CCCCCHHHHHHHCCCCHHEEECCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ADIDSDAVVVVAAVLFAIATVGINVVLNFVSPSYDISNVAPKYISFRTGGIITAVLSVAV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHEEECCHHHHHHHHHHH
LPWKIWGNEAAIVYFLGGVGALMGPVFGILVVDYYLLRRQHTHTADLYSESPGGAYHYAG
HHHEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEECCC
GFNPAALISLGIGGIVAVVIALVPRAQGVTALSWPVGALLGGVLMYLLGRGRSRSSRVGP
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCC
A
C
>Mature Secondary Structure
MVLEAAGPAAGRLSNDDLAPTTAAQATWRTWDIFCLWMTSAHSLGSYGFAVGMLALGLTG
CEECCCCCCCCCCCCCCCCCCHHHHCCCCHHEEEEEEEHHHHCCCHHHHHHHHHHHHHHH
WQTMLALFTGVLVLWTGSNLIGIAGQRLGVPFPVFARATFGVFGANIPALLRGLVAIFWY
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
GIQTYLASTAVIVLLLRLDPGLRSWTEPTFLGLHALGWAAFLSLWTVQLLILNYGMEVVR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KASDVAGPVVWIGMLALAVWMLDSVGWSIDFGARTGAPLSAGATVLAFVGVAFLMVAFMA
HHHHHCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
GPLVNYADFARFAPSRRAILRGNALGIPFNETAFVVISIVISLAALKKYGSAMTDPVELV
CCCCCHHHHHHHCCCCHHEEECCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ADIDSDAVVVVAAVLFAIATVGINVVLNFVSPSYDISNVAPKYISFRTGGIITAVLSVAV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHEEECCHHHHHHHHHHH
LPWKIWGNEAAIVYFLGGVGALMGPVFGILVVDYYLLRRQHTHTADLYSESPGGAYHYAG
HHHEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEECCC
GFNPAALISLGIGGIVAVVIALVPRAQGVTALSWPVGALLGGVLMYLLGRGRSRSSRVGP
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]