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The map label for this gene is pgk

Identifier: 169629862

GI number: 169629862

Start: 2824887

End: 2826125

Strand: Reverse

Name: pgk

Synonym: MAB_2778c

Alternate gene names: 169629862

Gene position: 2826125-2824887 (Counterclockwise)

Preceding gene: 169629863

Following gene: 169629861

Centisome position: 55.77

GC content: 65.29

Gene sequence:

>1239_bases
ATGGCGATCAAGTCCCTCAACGACCTGTTGGCCGAGGGAGTTTCGGGCAAGGGCGTGCTGGTCCGGTCGGACCTGAACGT
CCCGCTCGAGTACCTGGACGGCAACATCGCCCATATCTCTGATCCCGGTCGCATCGTGGCCTCGGTGCCGACCATCCGCG
CGCTTGCGGGTGCCGGGGCCAAGGTCATCGTGACCGCGCACCTGGGCCGCCCCGATGGAAAGCCGGACCCAAAACTGTCC
CTGGCCCCGGTCGGGGCGGCGTTGGGTGAACTGCTGGGGCAGCACGTGCAGGTGGCCGGAGACGTCGTCGGGACCGACGC
GCTGGCACGCGCCGAGGGCCTGACCGACGGCGATGTGCTGCTACTGGAGAACATCCGGTTCGACCCCCGGGAAACCAGCA
AGGACGACGCACAGCGTCTCGCGCTCGCGCGGGAACTGGCCGAGTTGGTCGGCGGCCCAACGGGCACCGGCGGCGCGTTC
GTTTCCGACGGATTCGGGGTAGTGCACCGTAAGCAGGCGTCCGTGTACGACGTGGCGACCTTGCTGCCGCATTACGCCGG
TGGTCTGGTGGCTGCCGAGGTGGAAGTTCTGCGGGTGCTCACGGCCAGTACCGAGCGGCCTTATGCGGTGGTTCTGGGCG
GGTCCAAGGTCTCCGACAAACTGGCGGTCATCGAATCGTTGGCTACCAAGGCCGACAGCCTGGTGATCGGCGGCGGCATG
TGCTTCACATTCCTGGCCGCGCAAGGTCTGCCGGTGGGCAAGTCGTTGGTACAGCTGGAGATGGTTGACACCTGCAAGCG
GCTGCTCGATACCTATGCCGATGTGATTCATCTGCCGATGGATATCGTGGCGGCAGATGAGTTCGCCGCCGATTCACCTT
CGGAAATCGTTGCCGCGGACGCGATTCCGGACAGCAAGATGGGTCTGGACATCGGACCGGAATCGGTGAAGCGGTTCGCC
GCGGTGTTGTCCAACGCCAAGACCATCTTCTGGAACGGCCCCATGGGCGTGTTCGAGTTCCCGGCGTTCGCCGCGGGTAC
CCGGGGTGTCGCCGAGGCGATCATCGCTGCTACGGAGAAGGGTGCGTTCAGCGTGGTCGGTGGCGGCGATTCGGCTGCCG
CGGTGCGCGCCCTGGACCTGCCCGATGATGGTTTCTCGCACATCTCCACCGGTGGTGGTGCATCCCTGGAATACCTTGAA
GGCAAGGTGCTTCCGGGTATCGACGTTCTCGAGGACTAG

Upstream 100 bases:

>100_bases
TCGACGACCAGGCCAAGGTGGTCTCCTGGTACGACAACGAGTGGGGCTACTCGAACCGCCTCGTCGACCTGGTCGGCCTG
GTCGGCAAGTCGCTGTAACG

Downstream 100 bases:

>100_bases
AGGAGTTCCGTATGTCGCGTAAGCCGTTGATCGCCGGCAACTGGAAGATGAACCTCAATCATTTCGAGGCCATCGCTTTG
GTGCAGAAGATCGCGTTCTC

Product: phosphoglycerate kinase (PGK)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MAIKSLNDLLAEGVSGKGVLVRSDLNVPLEYLDGNIAHISDPGRIVASVPTIRALAGAGAKVIVTAHLGRPDGKPDPKLS
LAPVGAALGELLGQHVQVAGDVVGTDALARAEGLTDGDVLLLENIRFDPRETSKDDAQRLALARELAELVGGPTGTGGAF
VSDGFGVVHRKQASVYDVATLLPHYAGGLVAAEVEVLRVLTASTERPYAVVLGGSKVSDKLAVIESLATKADSLVIGGGM
CFTFLAAQGLPVGKSLVQLEMVDTCKRLLDTYADVIHLPMDIVAADEFAADSPSEIVAADAIPDSKMGLDIGPESVKRFA
AVLSNAKTIFWNGPMGVFEFPAFAAGTRGVAEAIIAATEKGAFSVVGGGDSAAAVRALDLPDDGFSHISTGGGASLEYLE
GKVLPGIDVLED

Sequences:

>Translated_412_residues
MAIKSLNDLLAEGVSGKGVLVRSDLNVPLEYLDGNIAHISDPGRIVASVPTIRALAGAGAKVIVTAHLGRPDGKPDPKLS
LAPVGAALGELLGQHVQVAGDVVGTDALARAEGLTDGDVLLLENIRFDPRETSKDDAQRLALARELAELVGGPTGTGGAF
VSDGFGVVHRKQASVYDVATLLPHYAGGLVAAEVEVLRVLTASTERPYAVVLGGSKVSDKLAVIESLATKADSLVIGGGM
CFTFLAAQGLPVGKSLVQLEMVDTCKRLLDTYADVIHLPMDIVAADEFAADSPSEIVAADAIPDSKMGLDIGPESVKRFA
AVLSNAKTIFWNGPMGVFEFPAFAAGTRGVAEAIIAATEKGAFSVVGGGDSAAAVRALDLPDDGFSHISTGGGASLEYLE
GKVLPGIDVLED
>Mature_411_residues
AIKSLNDLLAEGVSGKGVLVRSDLNVPLEYLDGNIAHISDPGRIVASVPTIRALAGAGAKVIVTAHLGRPDGKPDPKLSL
APVGAALGELLGQHVQVAGDVVGTDALARAEGLTDGDVLLLENIRFDPRETSKDDAQRLALARELAELVGGPTGTGGAFV
SDGFGVVHRKQASVYDVATLLPHYAGGLVAAEVEVLRVLTASTERPYAVVLGGSKVSDKLAVIESLATKADSLVIGGGMC
FTFLAAQGLPVGKSLVQLEMVDTCKRLLDTYADVIHLPMDIVAADEFAADSPSEIVAADAIPDSKMGLDIGPESVKRFAA
VLSNAKTIFWNGPMGVFEFPAFAAGTRGVAEAIIAATEKGAFSVVGGGDSAAAVRALDLPDDGFSHISTGGGASLEYLEG
KVLPGIDVLED

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=40.632603406326, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=38.9294403892944, Blast_Score=261, Evalue=6e-70,
Organism=Escherichia coli, GI1789294, Length=415, Percent_Identity=41.4457831325301, Blast_Score=270, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17508823, Length=420, Percent_Identity=42.1428571428571, Blast_Score=291, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=407, Percent_Identity=38.5749385749386, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17136394, Length=414, Percent_Identity=39.1304347826087, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=40.2877697841727, Blast_Score=256, Evalue=3e-68,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_MYCA9 (B1MC86)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703511.1
- ProteinModelPortal:   B1MC86
- SMR:   B1MC86
- EnsemblBacteria:   EBMYCT00000006430
- GeneID:   5965286
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2778c
- GeneTree:   EBGT00050000017271
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- BioCyc:   MABS561007:MAB_2778C-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42265; Mature: 42133

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 44-44 BINDING 126-126 BINDING 170-170 BINDING 220-220 BINDING 308-308 BINDING 339-339

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKSLNDLLAEGVSGKGVLVRSDLNVPLEYLDGNIAHISDPGRIVASVPTIRALAGAGA
CCCCHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCEEEECCCCCEEECCCHHHHHHCCCC
KVIVTAHLGRPDGKPDPKLSLAPVGAALGELLGQHVQVAGDVVGTDALARAEGLTDGDVL
EEEEEEECCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEE
LLENIRFDPRETSKDDAQRLALARELAELVGGPTGTGGAFVSDGFGVVHRKQASVYDVAT
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEECCCHHHHHHH
LLPHYAGGLVAAEVEVLRVLTASTERPYAVVLGGSKVSDKLAVIESLATKADSLVIGGGM
HHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCHH
CFTFLAAQGLPVGKSLVQLEMVDTCKRLLDTYADVIHLPMDIVAADEFAADSPSEIVAAD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCHHCCCCCCCEEEEC
AIPDSKMGLDIGPESVKRFAAVLSNAKTIFWNGPMGVFEFPAFAAGTRGVAEAIIAATEK
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHCCCHHCCCHHHHHHHHHHCCC
GAFSVVGGGDSAAAVRALDLPDDGFSHISTGGGASLEYLEGKVLPGIDVLED
CCEEEEECCCCCCEEEEECCCCCCHHHCCCCCCCEEHHHCCCCCCCCHHCCC
>Mature Secondary Structure 
AIKSLNDLLAEGVSGKGVLVRSDLNVPLEYLDGNIAHISDPGRIVASVPTIRALAGAGA
CCCHHHHHHHCCCCCCEEEEECCCCCCHHHCCCCEEEECCCCCEEECCCHHHHHHCCCC
KVIVTAHLGRPDGKPDPKLSLAPVGAALGELLGQHVQVAGDVVGTDALARAEGLTDGDVL
EEEEEEECCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEE
LLENIRFDPRETSKDDAQRLALARELAELVGGPTGTGGAFVSDGFGVVHRKQASVYDVAT
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEECCCHHHHHHH
LLPHYAGGLVAAEVEVLRVLTASTERPYAVVLGGSKVSDKLAVIESLATKADSLVIGGGM
HHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCHH
CFTFLAAQGLPVGKSLVQLEMVDTCKRLLDTYADVIHLPMDIVAADEFAADSPSEIVAAD
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCHHCCCCCCCEEEEC
AIPDSKMGLDIGPESVKRFAAVLSNAKTIFWNGPMGVFEFPAFAAGTRGVAEAIIAATEK
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHCCCHHCCCHHHHHHHHHHCCC
GAFSVVGGGDSAAAVRALDLPDDGFSHISTGGGASLEYLEGKVLPGIDVLED
CCEEEEECCCCCCEEEEECCCCCCHHHCCCCCCCEEHHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA